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EBI DbfetchID L06036; SV 1; linear; genomic DNA; STD; PRO; 3366 BP. XX AC L06036; XX DT 17-JUN-1993 (Rel. 36, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Bacillus stearothermophilus ornithine acetyltransferase (argJ) and DE acetylglutamate kinase (argB) genes, complete cds's, argC gene, 3' end, and DE argD gene, 5' end. XX KW acetyl cycle; acetyl-CoA acetyltransferase; acetylglutamate kinase; KW acetylglutamate phosphotransferase; argB gene; argC gene; argD gene; KW arginine biosynthesis; argJ gene; glutamate acetyltransferase; KW ornithine acetyltransferase. XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-3366 RX PUBMED; 8473852. RA Sakanyan V., Charlier D., Legrain C., Kochikyan A., Mett I., Pierard P., RA Glansdorff N.; RT "Primary structure, partial purification and regulation of key enzymes of RT the acetyl cycle of arginine biosynthesis in Bacillus stearothermophilus: RT dual function of ornithine acetyltransferase"; RL J. Gen. Microbiol. 139(3):393-402(1993). XX FH Key Location/Qualifiers FH FT source 1..3366 FT /organism="Geobacillus stearothermophilus" FT /strain="NCIB 8224" FT /mol_type="genomic DNA" FT /db_xref="taxon:1422" FT CDS <1..885 FT /codon_start=1 FT /transl_table=11 FT /gene="argC" FT /db_xref="GOA:Q07906" FT /db_xref="InterPro:IPR012280" FT /db_xref="UniProtKB/Swiss-Prot:Q07906" FT /protein_id="AAA22196.1" FT /translation="VDGAVLHKLEIEALAKYDAVFFATPPGVSGEWAPALVDRGVKVID FT LSGDFRLKDGAVYAQWYGREAAPSAYLERAVYGLTEWNREAVRGAVLLSNPGCYPTATL FT LGLAPLVKEGLIKEDSIIVDAKSGVSGAGRKAGLGTHFSEVNENVKIYKVNAHQHIPEI FT EQALQTWNEAVAPITFSTHLIPMTRGIMATIYAKAKQSISPNDLVDLYKTSYEGSPFVR FT IRQLGQFPATKDVYGSNYCDIGLAYDERTERVTVVSVIDNLMKGAAGQAVQNFNLMMGW FT DEAEGLRSLPIYP" FT CDS 902..2134 FT /codon_start=1 FT /transl_table=11 FT /gene="argJ" FT /standard_name="N2-acetyl-L-ornithine L-glutamate FT N-acetyltransferase" FT /standard_name="glutamate acetyltransferase" FT /product="ornithine acetyltransferase" FT /EC_number="2.3.1.35" FT /note="also bears acetyl-CoA:L-glutamate FT N-acetyltransferase activity" FT /db_xref="GOA:Q07908" FT /db_xref="InterPro:IPR002813" FT /db_xref="UniProtKB/Swiss-Prot:Q07908" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA22197.1" FT /translation="MTITKQTGQVTAVADGTVVTPEGFQAAGVNAGLRYSKNDLGVILC FT DVPASAAAVYTQSHFQAAPLKVTQASLAVEQKLQAVIVNRPCANACTGAQGLKDAYEMR FT ELCAKQFGLALHHVAVASTGVIGEYLPMEKIRAGIKQLVPGVTMADAEAFQTAILTTDT FT VMKRACYQTTIDGKTVTVGGAAKGSGMIHPNMATMLAFITTDANVSSPVLHAALRSITD FT VSFNQITVDGDTSTNDMVVVMASGLAGNDELTPDHPDWENFYEALRKTCEDLAKQIAKD FT GEGATKLIEVRVRGAKTDEEAKKIAKQIVGSNLVKTAVYGADANWGRIIGAIGYSDAEV FT NPDNVDVAIGPMVMLKGSEPQPFSEEEAAAYLQQETVVIEVDLHIGDGVGVAWGCDLTY FT DYVKINASYRT" FT CDS 2147..2923 FT /codon_start=1 FT /transl_table=11 FT /gene="argB" FT /standard_name="N-acetylglutamate 5-phosphotransferase" FT /product="acetylglutamate kinase" FT /EC_number="2.7.2.8" FT /db_xref="GOA:Q07905" FT /db_xref="HSSP:1GS5" FT /db_xref="InterPro:IPR001057" FT /db_xref="UniProtKB/Swiss-Prot:Q07905" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA22198.1" FT /translation="MGKTVVIKCGGSVLDELSPAFFASVNAMRKQGMEVVIVHGGGPEI FT GQMLKTLRVPSEFVNGLRKTTKDVLAVVEMVLSGKVNKQLASMLRQHGLPAVGVSGVDG FT GLLEAEPIDLAKLGYVGRVKTVRSQLLRTLLAAGYIPVISPLGIDQNGQTYNINADTAA FT GAVAAAIGASQLAFVTNVPGILRDGALVAEATAEMIERLIEDGVITGGMIPKVQAALSA FT LSDALPEVMIVSGKTTFYQNGTWHGTTIRKEVGVYL" FT CDS 2920..>3366 FT /codon_start=1 FT /transl_table=11 FT /gene="argD" FT /db_xref="GOA:Q07907" FT /db_xref="HSSP:1QJ3" FT /db_xref="InterPro:IPR015421" FT /db_xref="UniProtKB/Swiss-Prot:Q07907" FT /protein_id="AAA22199.1" FT /translation="MSALFPTYNRWNIAVQSAEGTVVTDVNGKQYLDFVSGIAVCNLGH FT RHPHVQKSIEQQLNQYWHVSNLFTIPIQEEVASLLVAHSAGDYVFFCNSGAEANEAALK FT LARKHTGRHKVITFRQSFHGRTFATMAATGQEKVHSGFGPLLPEF" XX SQ Sequence 3366 BP; 783 A; 833 C; 1087 G; 663 T; 0 other; gtcgatggtg cggtgttgca taaacttgaa atcgaggcgc ttgccaagta tgacgcggtc 60 tttttcgcca cgccgcccgg ggtgtcgggg gagtgggcgc cggcgctcgt ggatcgggga 120 gtgaaagtga tcgatttatc gggcgatttc cggttgaaag acggcgcggt gtacgcacaa 180 tggtatgggc gcgaggcggc gccatctgcg tatttagagc gggcggtgta cggattgacc 240 gagtggaacc gcgaggcggt gcgcggagcc gtgctcctat ccaatccggg ctgctatccg 300 acagcgacgc tgcttggctt ggcgccgctt gtcaaagaag gattaattaa ggaagactcg 360 atcatcgtgg acgccaaatc gggcgtatcg ggcgcgggac ggaaagccgg gctcgggaca 420 catttttccg aagtgaacga aaacgtgaaa atttataaag tgaacgccca ccagcacatt 480 ccggaaattg aacaagcgct gcagacgtgg aatgaagcgg tggcgccgat cacgttcagc 540 actcatttga ttccgatgac aaggggcatt atggcgacga tttatgcgaa ggcgaaacaa 600 tcgatttcgc caaacgactt ggtagatttg tataaaacaa gttatgaagg ttcgccgttt 660 gtccgcatcc gtcaacttgg acagtttcca gcgacaaaag acgtgtacgg ctcaaattat 720 tgcgacatcg gcctcgccta cgatgagcgg acggaacggg tgacggtcgt gtcggtgatc 780 gacaacttga tgaaaggcgc tgccgggcag gcggtgcaaa acttcaactt gatgatgggt 840 tgggatgagg cggagggcct ccgatccttg ccgatctatc cgtgagggaa ggggaaaaac 900 gatgacgatc acaaaacaaa cggggcaagt gacggcggtc gccgatggaa cagtggttac 960 gccggaagga tttcaagcgg ccggggtgaa tgccgggctg cgctattcga aaaacgattt 1020 aggggttatt ctatgcgacg tgcccgcttc ggcggcggcg gtgtatacgc aaagccattt 1080 tcaggcggcg ccgctcaaag tgacgcaggc gagcctcgct gtagaacaaa aattgcaggc 1140 ggtcatcgtc aacaggccgt gcgcgaacgc ctgcaccggt gcgcaagggc tcaaggacgc 1200 ttatgaaatg cgtgagttgt gcgcgaaaca gtttggcctg gcgctgcacc atgtggccgt 1260 cgcttcaacg ggcgtaatcg gggaatattt gccgatggaa aaaattcgcg ccggcatcaa 1320 acagcttgtt ccaggggtga cgatggcgga tgcggaggcg tttcaaacgg cgattttaac 1380 gaccgatacg gtgatgaagc gcgcttgtta ccaaacaacg atcgacggga aaacggtcac 1440 cgtcggcgga gcggcgaaag ggtcggggat gatccatccg aacatggcga cgatgctcgc 1500 attcatcacg acggatgcca atgtttcgtc gccggtgctg cacgcggcgc tgcggtcgat 1560 tacggacgtt tcgtttaacc aaattacggt cgacggcgat acgtcgacaa atgatatggt 1620 cgtcgtgatg gcaagcggtc ttgctggaaa tgatgagttg acgccggatc atccggactg 1680 ggaaaacttt tatgaggcgc tgcggaaaac gtgcgaagat ttggcgaagc aaatcgccaa 1740 agacggcgag ggggcgacga agctcattga agtgcgcgtg cgcggcgcga aaacggatga 1800 ggaagcgaaa aaaatcgcca agcaaatcgt cggctctaac ttagtgaaaa cggccgttta 1860 cggcgcggac gccaactggg ggcggatcat cggcgcgatc ggctattcgg atgccgaagt 1920 gaacccggac aacgtcgatg tcgccatcgg gccgatggtg atgctgaaag gaagcgagcc 1980 gcagccgttc tcggaagaag aagcggcggc gtatttgcaa caagagacgg tcgtcattga 2040 ggtcgatttg catataggcg atggtgtcgg cgttgcgtgg ggctgcgatt tgacatacga 2100 ttatgtgaaa attaacgcca gctatcggac gtaaggagag agaacgatgg ggaaaacggt 2160 cgtcatcaaa tgcggcggca gcgtgcttga tgagctgtct cccgcctttt ttgccagcgt 2220 gaacgcaatg cgaaaacaag ggatggaggt cgtcatcgtc cacggcggcg ggccggaaat 2280 cggacagatg ctcaaaacgc ttcgcgtgcc gagcgagttt gtcaacggct tgcggaagac 2340 gacgaaagac gtgctcgcgg ttgtggaaat ggtgctctcc ggcaaagtga acaaacagct 2400 tgcgtcgatg ctcaggcagc acggtttgcc cgcggtcggt gtttccggcg tggacggggg 2460 gctgcttgaa gcagaaccga tcgacttggc caaactcggc tatgtcggcc gtgtgaaaac 2520 cgttcgttcc cagctgttgc gcacgctgct tgcggcgggc tacatcccgg tcatttcccc 2580 gcttggcatc gaccaaaacg ggcaaacgta caacattaac gccgacacgg ccgccggggc 2640 ggtcgcagcg gccatcggcg ccagccagct cgcgtttgtg acgaacgtgc ccggcatttt 2700 gcgagacggc gcgctcgtgg ccgaggcgac ggcggaaatg attgaacgac tgatcgaaga 2760 cggcgtcatc acgggcggga tgatcccgaa agtgcaagcg gcgctctccg ccctatccga 2820 tgcgctgccg gaagtgatga tcgtcagcgg caaaacgacg ttttaccaga acggaacatg 2880 gcacggcaca acgattcgca aagaagtggg ggtatattta tgagcgcgct gtttccgact 2940 tacaaccgtt ggaatatcgc cgtccagtcg gccgaaggga cggtggtgac cgatgtgaat 3000 ggaaaacaat atctcgactt tgtttccggc attgccgtct gcaatctcgg ccatcgccat 3060 ccgcatgtcc aaaagagcat tgaacaacag ctgaatcaat attggcacgt ctcgaacttg 3120 tttacgatcc cgattcaaga agaggtcgcc tccttgcttg tcgcccacag cgccggcgat 3180 tatgtttttt tctgcaacag cggggcggaa gcgaacgagg cggcgctgaa gctggcgcgc 3240 aaacataccg ggagacacaa agtcattacg ttccgccagt cgtttcacgg ccgcacgttc 3300 gcgacgatgg cggcgaccgg gcaggagaaa gtgcatagcg gttttggtcc gctgcttccg 3360 gaattc 3366 // |
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