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EBI DbfetchID L05611; SV 1; linear; genomic DNA; STD; PRO; 2964 BP. XX AC L05611; XX DT 12-NOV-1992 (Rel. 34, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 8) XX DE Bacillus stearothermophilus class I Fe-S fumarase (fumA) gene, complete DE cds. XX KW class I Fe-S fumarase; fumA gene; fumarase. XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-2964 RX PUBMED; 8473853. RA Reaney S.K., Begg C., Bungard S., Guest J.R.; RT "Molecular and enzymological evidence for two classes of fumarase in RT Bacillus stearothermophilus (var. non-diastaticus)"; RL J. Gen. Microbiol. 139(3):403-416(1993). XX FH Key Location/Qualifiers FH FT source 1..2964 FT /organism="Geobacillus stearothermophilus" FT /mol_type="genomic DNA" FT /tissue_lib="DSM22334" FT /db_xref="taxon:1422" FT TATA_signal 115..121 FT /gene="fumA(Bst)" FT gene 115..1761 FT /gene="fumA(Bst)" FT RBS 156..164 FT /gene="fumA(Bst)" FT CDS 174..1718 FT /codon_start=1 FT /transl_table=11 FT /gene="fumA(Bst)" FT /product="fumarase" FT /EC_number="4.2.1.2" FT /db_xref="GOA:Q04718" FT /db_xref="InterPro:IPR004647" FT /db_xref="UniProtKB/Swiss-Prot:Q04718" FT /protein_id="AAA72317.1" FT /translation="MEKLYESMYQLIVETSTKLPKDVRRAILKAKQRENAGTRAAMSLA FT TITENIKMADENVSPICQDTGLPTFKIKVPVGINQIQIKETIKKAIAQATKDGKLRPNS FT VDSLTGENSGDNLGEGLPVVKFEQWEKDYMDVRLILKGGGCENKNIQYSLPCELEGLGR FT AGRDLDGIRKCILHAVYQAQGQGCSAGFIGVGIGGDRSAGYDLAKEQLFREVDDVNPNE FT ELRQLEEYIMENANKLGIGTMGFGGETTLLGCKVGAMHRIPASFFVSVAYNCWAFRRLG FT VHIDPNTGEIIKWLYQDGEDVDFQENAAQEEHLASTDSERRVITLQAPITEEQIRELKV FT GDVVRINGIIYTGRDAIHKYLMDHDAPVDLNGQIIYHCGPVMLKDENGNWEVKAAGPTT FT SIREEPYQGDIMKKFGIRAVMGKGGMGQKTLQALKEHGGVYLNAIGGAAQYYADCIEAV FT EGVDLLEFGIPEAMWHLRVKDFTAVVTMDSHGNSLHEDIEKSSLEKLSQFKEPVFS" FT terminator 1729..1761 FT /gene="fumA(Bst)" FT RBS 1827..1832 FT CDS 1842..2639 FT /codon_start=1 FT /transl_table=11 FT /label=ORF2 FT /db_xref="GOA:Q04729" FT /db_xref="HSSP:1NY1" FT /db_xref="InterPro:IPR014235" FT /db_xref="UniProtKB/Swiss-Prot:Q04729" FT /protein_id="AAA72318.1" FT /translation="MNYFRILYCSVLLFFSFFSCTSAAVSNHPIHWGFQKNNNHQPPDA FT GKEWNELLNKYGAFYLGDPSKKEIYLTFDNGYENGYTSKILDVLKKHDVHATFFVTGHY FT LKTAPDLVKRMVKEGHIVGNHSWSHPDMTTISADKIKKELDAVSDKVKELTGQEGTVYV FT RPPRGIFSERTLALSEKYGYRNIFWSLAFVDWKVNEQKGWRYAYDNIINQIHPGAIILL FT HTVSKDNADALDEAIKTLKQQGYTFKSLDDLVMEKMFESPFLY" FT CDS 2699..>2964 FT /codon_start=1 FT /transl_table=11 FT /label=ORF3 FT /db_xref="UniProtKB/Swiss-Prot:Q04730" FT /protein_id="AAA72319.1" FT /translation="MWNVTIKAAPPYDFDRVLERLSLDPLNKVDVHKRTVLVPLYSEKE FT EPFVAVVKAIGSKENPIFEISGEQDEQKERAIHELTRIFQWKNS" XX SQ Sequence 2964 BP; 977 A; 533 C; 705 G; 749 T; 0 other; gaattcgcaa gctgaatctt ttttaactgc tgcaatgaat gctgaaacac tttccctatc 60 cccctaaaaa aatgatcagc acgtgaatgc ctttccaatc aaaattaaaa gaagtataat 120 aaaaattgtg ttaagtggga gttttttgac tgaatgtaag ggggataagc gggatggaaa 180 aattatatga gagtatgtat caattaattg tggaaacgtc tacaaaattg ccgaaggatg 240 tccgccgcgc tattttgaag gcgaagcagc gtgaaaatgc tggtacaaga gctgcgatgt 300 cgcttgccac gataaccgaa aatattaaaa tggcagatga aaatgtatcg ccgatctgcc 360 aagataccgg attgccgaca tttaaaatta aagtgccagt cggtatcaat caaattcaaa 420 taaaggaaac aataaaaaaa gcgatcgctc aagcgacgaa ggacgggaag cttcgcccga 480 actcggtcga ttcactcact ggagaaaatt ccggcgataa cttgggcgaa gggcttccag 540 tcgtaaaatt tgaacaatgg gaaaaagatt atatggatgt tcgcttaata ttaaaaggcg 600 gcggctgcga aaacaaaaat attcaataca gcctgccgtg cgaactagaa ggattgggaa 660 gagccggcag agatttggat ggaatccgca aatgtatttt gcatgcggta tatcaagcac 720 agggccaagg atgcagcgca ggattcattg gagttggaat cggtggagac cgttcagcgg 780 ggtacgattt agcgaaagaa caattattcc gcgaagtcga tgacgtaaat ccgaacgaag 840 aattgcggca attggaagaa tatattatgg aaaatgcaaa caagcttggc attggaacga 900 tgggatttgg cggggaaacc actttattag gctgcaaagt cggcgcaatg cacagaattc 960 ctgcgagctt ttttgtttct gtcgcataca actgctgggc attccgccgt ttaggtgtgc 1020 atatcgatcc aaatacgggt gaaatcataa aatggcttta tcaagatgga gaagatgttg 1080 attttcaaga aaatgctgca caagaagaac atttagcatc aacggactct gaaagacgtg 1140 tcatcacgct gcaagcgcca attacagaag aacaaatccg cgagctgaaa gtcggcgacg 1200 ttgttcgcat caacggaatc atttacacag gaagggatgc catccataaa tatttaatgg 1260 atcatgatgc gccggttgat ttaaacggtc aaattattta ccactgcgga cctgtcatgt 1320 taaaagatga aaacggcaat tgggaagtga aagcggccgg gccgaccact tccattcgcg 1380 aagagcctta tcaaggggat attatgaaaa aatttggcat ccgtgcggta atgggcaaag 1440 gcggcatggg gcaaaaaacg ctgcaagctt taaaagagca tggcggagta tacttgaatg 1500 cgattggcgg tgctgctcaa tattatgcgg attgcatcga agcggttgaa ggtgttgact 1560 tgctggagtt tggaattcca gaagctatgt ggcatttgcg cgtaaaagac ttcacggccg 1620 ttgtcacaat ggattcacac ggcaacagcc tgcatgaaga cattgaaaaa tcgtcattag 1680 aaaagctttc tcaatttaaa gagccggtat ttagctaaat attaaactga atgccatagc 1740 cagatgattg gctatggtat ttttttgaaa tcaagatgga gcataattcc taaaaacgga 1800 taaagactaa tagtaaaaat aatggaaagg agattcacag cttgaattat ttccggattt 1860 tatattgctc tgtcttgtta tttttttctt tttttagctg cacctctgct gctgtcagca 1920 atcacccgat tcattggggt tttcaaaaaa acaataatca tcagcctcct gatgccggga 1980 aagaatggaa tgaactttta aataaatatg gagcgtttta tttaggagat cccagcaaaa 2040 aagagattta tttaacgttt gataatggct atgaaaacgg ctatacatcg aaaattttgg 2100 acgtactgaa aaagcacgat gtacatgcga cgttttttgt aacaggccat tatttaaaaa 2160 cagcccctga tcttgtaaag agaatggtga aggaagggca tattgtcggc aatcattcat 2220 ggagccaccc tgatatgacg acaataagtg ctgataaaat aaagaaggag cttgatgccg 2280 tcagcgacaa agtaaaggag cttacaggcc aagaaggaac agtatatgtg agaccgccaa 2340 ggggcatttt tagcgaacgc acacttgcat tgtccgaaaa atacggttat cgcaatattt 2400 tttggtcgct tgcatttgtc gactggaagg tgaatgaaca aaaaggctgg cgatatgcat 2460 acgacaacat tatcaatcaa attcatccgg gcgcgatcat tttgctgcac accgtatcaa 2520 aagacaacgc tgatgcgctt gatgaagcga ttaaaacttt aaaacagcaa ggctatactt 2580 ttaaaagttt agacgatcta gtgatggaga aaatgtttga aagtccattt ctttattaat 2640 agaagcggat tatgtgatat aatagctccg aaaaaatacg ataaaggtcg gtaaaaacat 2700 gtggaatgtg acgataaaag cggcgccgcc gtatgacttc gaccgcgtgt tagagcgcct 2760 ttcacttgat ccgttgaaca aggtggacgt gcacaagcga accgtccttg ttcctcttta 2820 tagtgaaaaa gaagaacctt ttgttgccgt tgtaaaagca atcggatcga aggaaaaccc 2880 aatatttgaa atatcagggg aacaggatga acaaaaagaa cgagccatcc atgagctgac 2940 acgcatattt caatggaaga attc 2964 // |
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