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EBI DbfetchID L04735; SV 1; linear; genomic DNA; STD; PRO; 3048 BP. XX AC L04735; XX DT 29-OCT-1992 (Rel. 33, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Clostridium thermocellum (clone RV 1.6) endo-1, 3-beta-glucanase (CMC) DE gene, complete cds. XX KW cellulase; endo-beta-1,3-glucanase. XX OS Clostridium thermocellum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-3048 RX PUBMED; 8436949. RA Hazlewood G.P., Davidson K., Laurie J.I., Huskisson N.S., Gilbert H.J.; RT "Gene sequence and properties of CelI, a family E endoglucanase from RT Clostridium thermocellum"; RL J. Gen. Microbiol. 139(2):307-316(1993). XX FH Key Location/Qualifiers FH FT source 1..3048 FT /organism="Clostridium thermocellum" FT /mol_type="genomic DNA" FT /clone="RV 1.6" FT /tissue_lib="pBR322; NCIB 10682" FT /db_xref="taxon:1515" FT RBS 245..251 FT /gene="CMC" FT /note="putative" FT sig_peptide 256..417 FT /gene="CMC" FT /experiment="experimental evidence, no additional details FT recorded" FT CDS 256..2895 FT /codon_start=1 FT /transl_table=11 FT /gene="CMC" FT /product="endo-1,3-beta-glucanase" FT /function="cellulase" FT /EC_number="3.2.1.4" FT /db_xref="GOA:Q02934" FT /db_xref="InterPro:IPR001701" FT /db_xref="InterPro:IPR001956" FT /db_xref="InterPro:IPR008928" FT /db_xref="InterPro:IPR008965" FT /db_xref="InterPro:IPR012341" FT /db_xref="InterPro:IPR018221" FT /db_xref="UniProtKB/Swiss-Prot:Q02934" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA20892.1" FT /translation="MRLVNSLGRRKILLILAVIVAFSTVLLFAKLWGRKTSSTLDEVGS FT KTHGDLTAENKNGGYLPEEEIPDQPPATGAFNYGEALQKAIFFYECQRSGKLDPSTLRL FT NWRGDSGLDDGKDAGIDLTGGWYDAGDHVKFNLPMSYSAAMLGWAVYEYEDAFKQSGQY FT NHILNNIKWACDYFIKCHPEKDVYYYQVGDGHADHAWWGPAEVMPMERPSYKVDRSSPG FT STVVAETSAALAIASIIFKKVDGEYSKECLKHAKELFEFADTTKSDDGYTAANGFYNSW FT SGFYDELSWAAVWLYLATNDSSYLDKAESYSDKWGYEPQTNIPKYKWAQCWDDVTYGTY FT LLLARIKNDNGKYKEAIERHLDWWTTGYNGERITYTPKGLAWLDQWGSLRYATTTAFLA FT CVYSDWENGDKEKAKTYLEFARSQADYALGSTGRSFVVGFGENPPKRPHHRTAHGSWAD FT SQMEPPEHRHVLYGALVGGPDSTDNYTDDISNYTCNEVACDYNAGFVGLLAKMYKLYGG FT SPDPKFNGIEEVPEDEIFVEAGVNASGNNFIEIKAIVNNKSGWPARVCENLSFRYFINI FT EEIVNAGKSASDLQVSSSYNQGAKLSDVKHYKDNIYYVEVDLSGTKIYPGGQSAYKKEV FT QFRISAPEGTVFNPENDYSYQGLSAGTVVKSEYIPVYDAGVLVFGREPGSASKSTSKDN FT GLSKATPTVKTESQPTAKHTQNPASDFKTPANQNSVKKDQGIKGEVVLQYANGNAGATS FT NSINPRFKIINNGTKAINLSDVKIRYYYTKEGGASQNFWCDWSSAGNSNVTGNFFNLSS FT PKEGADTCLEVGFGSGAGTLDPGGSVEVQIRFSKEDWSNYNQSNDYSFKQACLRQRTLI FT YLYATWLR" FT mat_peptide 418..2892 FT /gene="CMC" FT /product="endo-1,3-beta-glucanase" XX SQ Sequence 3048 BP; 957 A; 529 C; 757 G; 805 T; 0 other; ggtaaatact aggaaaacgt aaaataaaaa tacgtaaagc agagtgaatt ttgacattta 60 ctctgctttt ttaaaatacc aaaaaaggca tgtccgaaaa atttttgaaa tctatacggc 120 aatttttctg aaatttggtt ttatcaaatt tgacgaagca tatatgatat aataaactaa 180 taaagcggat aaatatagat ttacttaaaa ttaaagttgt aaattgattt ttattaattg 240 aggcaggagg atgctatgag gctggttaac agtttgggaa gaagaaaaat tcttttgata 300 cttgctgtta ttgtagcttt cagcactgtt ctgttgtttg caaagctatg ggggcgaaag 360 acttcaagta ctttggatga ggttggttca aaaactcatg gtgatttgac ggcagaaaat 420 aaaaacggcg gatatttacc agaggaagag attccagatc agcctccggc aaccggggcc 480 ttcaactacg gtgaagcgtt gcaaaaagca atttttttct atgagtgtca aagatccgga 540 aagctcgatc cctcaactct tcgcctaaat tggcggggag attcgggact ggatgacgga 600 aaagatgcag gaattgatct taccggcgga tggtatgatg cgggagatca cgtaaaattt 660 aatttgccca tgtcttattc ggcggctatg ctggggtggg cggtgtatga atatgaagat 720 gcgtttaaac agagtggaca gtataaccac atattgaata acataaaatg ggcttgtgat 780 tattttataa aatgtcatcc ggaaaaggat gtgtactatt accaggtggg cgacggtcat 840 gctgaccatg cgtggtgggg tcctgccgaa gtaatgccta tggaaaggcc gtcgtacaaa 900 gttgacaggt catcgccggg ttccacggtt gtggcagaga cgtcggcagc tttagctata 960 gcatcgataa tatttaagaa agtggatggt gaatactcga aagaatgttt aaagcatgca 1020 aaagaactgt ttgaatttgc ggacaccaca aaaagcgatg atgggtacac tgcagccaat 1080 ggtttttata attcatggag cggattttat gatgagcttt cctgggcagc tgtatggctt 1140 tatcttgcta ccaatgattc ttcatatttg gataaagcgg aaagttattc tgacaaatgg 1200 ggttatgagc cacagacgaa cataccgaag tataagtggg ctcaatgctg ggatgatgtg 1260 acttatggca cttatcttct tttggccagg attaaaaatg acaacggaaa atataaagaa 1320 gcgatagaaa ggcatcttga ttggtggaca accggataca acggtgaaag aattacatat 1380 actccgaagg gacttgcatg gctcgaccag tggggatcat tgaggtatgc aaccacaacg 1440 gcatttctgg catgtgttta ttccgattgg gagaacggtg ataaggaaaa agcaaaaact 1500 tatctggagt ttgcaagaag ccaggcggat tatgctttgg gaagcacggg aagaagcttt 1560 gttgtgggtt ttggagaaaa tccaccgaaa aggccccatc acagaactgc tcacggttca 1620 tgggcggaca gtcagatgga gcctcccgaa cacaggcatg ttctttatgg tgcccttgtg 1680 ggaggacctg acagcacgga caactacacc gacgacatca gtaattacac ctgcaatgaa 1740 gttgcctgtg actataatgc aggttttgtg ggactgcttg caaaaatgta caagctttat 1800 ggcggaagtc ccgatcccaa atttaacggt atagaagaag ttccggagga tgaaatattc 1860 gttgaagccg gtgtgaatgc atcgggaaac aatttcattg aaataaaagc gatagttaat 1920 aataaatcgg gctggcctgc aagagtatgt gagaatttat cctttagata ttttatcaac 1980 attgaagaga ttgtgaatgc gggaaaaagt gcaagcgacc tgcaagtgag ctccagctac 2040 aatcaggggg caaaactgtc cgatgtaaag cactacaagg acaatattta ttatgtggaa 2100 gtggatttgt cggggacaaa aatatatccc gggggacaat cggcatacaa gaaggaagtg 2160 cagtttagaa tttccgcgcc ggagggcacg gtgtttaatc cggaaaacga ctattcctat 2220 cagggacttt cggcaggtac ggttgtaaag tctgagtata ttccggtata tgatgccggg 2280 gtgctggtat ttggaaggga accgggctca gcatcgaaaa gcacgtctaa agacaatggt 2340 ttgtccaagg caactcccac ggtgaaaact gaatctcagc cgacagcaaa acacactcaa 2400 aatcctgcct cagactttaa aactccagcc aatcagaaca gtgtaaaaaa agaccaaggc 2460 ataaaaggag aagtggtatt acagtacgca aacgggaatg caggtgctac gtcaaacagt 2520 attaatccga ggtttaaaat aattaacaac ggtacaaaag ccataaattt gtccgatgtc 2580 aagattagat attattacac aaaagaaggg ggcgcatctc aaaacttctg gtgtgattgg 2640 agcagtgccg gcaattcaaa tgttacagga aacttcttta atctttcttc accgaaagaa 2700 ggagcggaca cctgtcttga agttggtttc ggaagtgggg ccggaaccct tgatcctggt 2760 ggaagcgttg aagtacagat aaggttttca aaggaagact ggtcaaacta taaccagtca 2820 aacgattatt ctttcaaaca agcttgttta cggcaaagaa ccttgattta tctgtacgca 2880 acatggctaa gataggcttt tgcaataccg atgtaatatt cggattctct tacaatgtga 2940 ttaattacaa cagaggctgt caaattgttt gccactgcgg cactctcttt tgccatgcag 3000 tttaggaagt ttacgaaggt ttgactttga cgtagtgtta tttcgaat 3048 // |
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