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EBI DbfetchID L04507; SV 1; linear; genomic DNA; STD; PRO; 3574 BP. XX AC L04507; XX DT 07-JAN-1993 (Rel. 34, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 11) XX DE Klebsiella terrigena budABC operon, including alpha-acetolactate DE decarboxylase (budA), alpha-acetolactate synthase (budB), and DE acetoin(diacetyl)reductase (budC) genes, complete coding regions. XX KW acetoin(diacetyl)reductase; acetolactate decarboxylase; KW acetolactate synthase; budA gene; budABC operon; budB gene; budC gene. XX OS Raoultella terrigena OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Raoultella. XX RN [1] RP 1-3574 RX PUBMED; 8444801. RA Blomqvist K., Nikkola M., Lehtovaara P., Suihko M.-L., Airaksinen U., RA Straby K.B., Knowles J.K.C., Penttilae M.E.; RT "Characterization of the genes of the 2,3-butanediol operons from RT Klebsiella terrigena and Enterobacter aerogenes"; RL J. Bacteriol. 175(5):1392-1404(1993). XX FH Key Location/Qualifiers FH FT source 1..3574 FT /organism="Raoultella terrigena" FT /mol_type="genomic DNA" FT /tissue_lib="VTT-E-74023" FT /db_xref="taxon:577" FT mRNA 145..>3396 FT /note="budABC operon" FT RBS 166..170 FT /note="budABC operon" FT CDS 179..958 FT /codon_start=1 FT /transl_table=11 FT /gene="budA" FT /product="alpha-acetolactate decarboxylase" FT /EC_number="4.1.1.5" FT /db_xref="GOA:Q04518" FT /db_xref="InterPro:IPR005128" FT /db_xref="UniProtKB/Swiss-Prot:Q04518" FT /protein_id="AAA25054.1" FT /translation="MNHYPECTCQESLCETVRGFSAHHPDSVIYQTSLMSALLSGVYEG FT STTIADLLTHGDFGLGTFNELDGELIAFSSEVYQLRADGSARKARADQKTPFAVMTWFR FT PQYRKTFDHPVSRQQLHDVIDQQIPSDNLFCALHIDGHFRHAHTRTVPRQTPPYRAMTD FT VLDDQPVFRFNQRKGTLVGFRTPQHMQGLNVAGYHEHFITDDRQGGGHLLDYQLDSGVL FT TFGEIHKLMIDLPADSAFLQADLHPDNLDAAIRAVEN" FT CDS 969..2648 FT /codon_start=1 FT /transl_table=11 FT /gene="budB" FT /product="alpha-acetolactate synthase" FT /EC_number="4.1.3.18" FT /db_xref="GOA:Q04524" FT /db_xref="InterPro:IPR000399" FT /db_xref="InterPro:IPR011766" FT /db_xref="InterPro:IPR012000" FT /db_xref="InterPro:IPR012001" FT /db_xref="InterPro:IPR012782" FT /db_xref="UniProtKB/Swiss-Prot:Q04524" FT /protein_id="AAA25055.1" FT /translation="MDKPRHERQWAHGADLIVSQLEAQGVRQVFGIPGAKIDKVFDSLL FT DSSIRIIPVRHEANAAFMAAAVGRITGKAGVALVTSGPGCSNLITGMATANSEGDPVVA FT LGGAVKRADKAKLVHQSMDTVAMFSPVTKYAVEVTASDALAEVVSNAFRAAEQGRPGSA FT FVSLPQDIVDGPASGSTLPASRAPQMGAAPDGAVDSVAQAIAAAKNPIFLLGLMASQPE FT NSRALHRHAGKKPYSGHQHLSGAGAVNQDNFARFAGRVGLFNNQAGDRLLRQADLIICI FT GYSPVEYEPAMWNSGTATLVHIDVLPAYEERNYVPDIELVGDIAATLEKLAQRIEHRLV FT LTPQAADILADRQRQRELLDRRGAQLNQFALHPLRIVRAMQDIVNSDVTLTVDMGSFHI FT WIARYLYSFRARQVMISNGQQTMGVALPWAIGAWLVNPQRKVVSVSGDGGFLQSSMELE FT TAVRLHANILHIIWVDNGYNMVAIQEQKKYQRLSGVEFGPVDFKVYAEAFGACGFAVES FT AEALEPTLRAAMDVDGPAVVAIPVDYRDNPLLMGQLHLSQIL" FT CDS 2671..3396 FT /codon_start=1 FT /transl_table=11 FT /gene="budC" FT /product="acetoin(diacetyl)reductase" FT /db_xref="GOA:Q04520" FT /db_xref="InterPro:IPR002198" FT /db_xref="InterPro:IPR002347" FT /db_xref="InterPro:IPR014007" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:Q04520" FT /protein_id="AAA25056.1" FT /translation="MQKVALVTGAGQGIGKAIALRLVKDGFAVAIADYNDATATAVAAE FT INQAGGRAVAIKVDVSRRDQVFAAVEQARKALGGFNVIVNNAGIAPSTPIESITEEIVD FT RVYNINVKGVIWGMQAAVEAFKKEGHGGKIVNACSQAGHVGNPELAVYSSSKFAVRGLT FT QTAARDLAPLGITVNGFCPGIVKTPMWAEIDRQCRKRRANRWATARLNLPNASPLAACR FT SLKTSPPACRSSPARIPTI" XX SQ Sequence 3574 BP; 725 A; 1118 C; 1061 G; 670 T; 0 other; atcgtgggcc gcccgagtga agttccgcgc ctcggcaacg gcgacaaaat aacgtaaata 60 gcgaagttcc atatcgtaaa cgtctcaaac cagcatggat tctatattgg aactgagagc 120 tgaatcgggt caatatttat ttaatctttc ttatatttgt tgaacgagga agtggattgt 180 gaatcattat cctgaatgca cctgccagga gagcctgtgc gaaaccgtac gcggcttctc 240 cgcccaccac cctgatagcg ttatctatca gacctctctg atgagcgcgc tgctgagcgg 300 ggtctatgag ggtagcacca ccatcgccga cctgctgacc cacggcgact tcggtctcgg 360 cacctttaac gaactcgatg gcgaactgat tgcctttagc agcgaggtct accagctgcg 420 cgctgacggc agcgcgcgta aagcccgggc ggatcaaaaa acgcccttcg cggtgatgac 480 ctggttcaga ccgcagtacc gtaaaacctt tgaccacccg gtcagccgcc agcagctgca 540 cgacgttatc gaccagcaaa tcccctccga taacctgttc tgcgccctgc atattgatgg 600 tcactttcgc cacgcccaca cccgcaccgt gccgcggcag acgccgccct atcgggcgat 660 gaccgacgtg ctcgatgacc agccggtttt ccgcttcaac cagcgcaagg ggacgctggt 720 cggctttcgc accccgcagc atatgcaggg ccttaacgtt gccggctacc acgagcactt 780 tattaccgac gatcgccagg gcggcggcca tctgctggac taccagctcg atagcggcgt 840 gctgaccttc ggcgagatcc acaagctgat gattgacctc ccggccgaca gcgctttcct 900 gcaggccgac ctgcatcctg acaatctcga tgccgctatt cgtgcggtag aaaactaagg 960 agcttcagat ggacaaaccg cgtcacgaac gtcaatgggc ccacggtgcc gacttaatcg 1020 tcagccagct tgaggcccag ggcgtacgcc aggtcttcgg catccccggt gccaaaatcg 1080 acaaggtgtt tgattccctc ctcgactcct caatccgcat tattccggtg cgccacgagg 1140 ctaacgccgc ctttatggcc gcggcggtcg ggcggattac cggtaaagcg ggcgtcgcgc 1200 tggtgacctc cggtcccggc tgctcaaacc tgattaccgg catggccacc gccaatagcg 1260 aaggcgaccc ggtggtggcg ctgggcggcg cggtgaagcg cgcggataag gccaagctgg 1320 ttcaccaaag catggacacc gtggcgatgt tcagcccggt caccaaatac gccgtcgagg 1380 tgaccgcctc cgacgcgctg gccgaggtgg tctccaacgc ctttcgcgcc gccgaacagg 1440 ggcgtccggg gagcgcgttt gtcagcctgc cgcaggatat cgttgacggc cccgccagcg 1500 gcagcacgct gcccgccagc agagcgccgc agatgggcgc cgcgccggat ggcgccgttg 1560 acagcgtggc gcaggcgatc gccgcggcga agaaccctat cttcctgctc gggctgatgg 1620 ccagccagcc ggaaaacagc cgcgccctgc accgccatgc tggaaaaaag ccatattccg 1680 gtcaccagca cctatcaggc gccggggcgg taaatcagga taacttcgcc cgcttcgccg 1740 gccgggtagg cctgtttaat aaccaggcgg gcgatcgcct gctgcgtcag gcggacctga 1800 tcatctgcat cggctatagc ccggttgagt acgaaccggc gatgtggaac agcggcacgg 1860 caaccctggt gcatatcgac gtgctgccgg cctatgaaga gcggaactac gtcccggata 1920 tcgagctggt gggcgacatc gccgccaccc tcgagaagct ggcccagcgc attgaacatc 1980 ggctggtgtt aactccgcag gcggcggaca tcctcgccga ccgccagcgc cagcgggagc 2040 tgcttgaccg ccgcggggcg cagctgaatc agtttgcgct ccacccgctg cgcatcgtgc 2100 gggcgatgca ggatatcgtc aatagcgacg tcaccttgac cgtcgatatg ggcagtttcc 2160 atatctggat tgcccgctac ctctacagct tccgcgcccg ccaggtgatg atctccaacg 2220 gtcagcaaac gatgggcgtc gcgctgccgt gggcaatcgg cgcgtggctg gtcaatccgc 2280 agcgcaaggt ggtctcggta tccggcgatg gcggcttcct gcagtcgagc atggagctgg 2340 agaccgccgt gcgcctgcac gccaatattc tgcacatcat ctgggtcgat aacggctaca 2400 acatggtggc gattcaggaa cagaagaaat atcagcgcct ctccggcgtg gagttcggcc 2460 cggtcgattt caaagtctac gccgaagcgt tcggggcctg cgggtttgcg gtagagagcg 2520 ccgaggccct ggagccgacc ctgcgcgcgg cgatggatgt cgacggcccg gcggtggtcg 2580 ccattccggt cgattaccgc gataaccctc tgctgatggg ccagctccat ctcagccaaa 2640 tactgtgagt cactacaaaa ggaaatgaaa atgcaaaaag tcgcacttgt caccggcgcc 2700 ggtcagggca tcggtaaagc tatcgccctg cgtctggtga aggatggatt tgccgtggca 2760 atcgccgatt acaacgacgc tacggccaca gcggtagccg ctgaaatcaa ccaggccggc 2820 ggccgcgcgg tggccattaa ggtcgacgtc tcgcgccggg accaggtttt cgccgccgtt 2880 gagcaggcgc gtaaagccct gggcggattc aacgttatcg tcaacaacgc cggcatcgcg 2940 ccgtcaacgc cgatcgagtc catcaccgag gagatcgtcg accgggtcta taacatcaac 3000 gttaagggcg tcatctgggg gatgcaggcg gcggtggagg ccttcaaaaa agaggggcac 3060 ggcgggaaga tcgtcaacgc ctgctcccag gccggccacg tcggcaaccc ggagctggcg 3120 gtctacagtt cgagtaaatt cgccgtgcgc ggcctgacgc aaaccgccgc ccgcgatctg 3180 gcgccgctgg gcatcaccgt taacggcttc tgcccaggga tcgttaagac gccaatgtgg 3240 gcggagattg accgtcagtg tcggaagcgg cgggcaaacc gctgggctac ggcacggctg 3300 aatttgccaa acgcatcacc cttggccgcc tgtcggagcc tgaagacgtc gccgcctgcg 3360 tgtcgttcct cgccagcccg gattccgact atatgaccgg ccaatcgctg ctgatcgacg 3420 gcggcatggt attcaactaa tcgtagtatt taattaatcg tggtattcaa ctaataatat 3480 taagctctga catgatttgc cctgatctaa cgcaggggcc tttttatgcc ctatcccacc 3540 gccagtgcaa acaaatcagc ccttcacaat ctcc 3574 // |
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