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EBI DbfetchID L04500; SV 1; linear; genomic DNA; STD; PRO; 6736 BP. XX AC L04500; XX DT 28-NOV-1993 (Rel. 37, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 11) XX DE Eubacterium acidaminophilum selenoprotein B of glycine-reductase (grdB) DE gene, partial cds; thioredoxin reductase (trxB), thioredoxin (trxA), DE selenoprotein A of glycine-reductase (grdA), glycine reductase (grdC), and DE glycine reductase (grdD) genes, complete cds. XX KW . XX OS Eubacterium acidaminophilum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Eubacteriaceae; OC Eubacterium. XX RN [1] RC Erratum:[[corrected] [published erratum appears in J Bacteriol 1992 RC Feb;174(4):1432]] RP 1-6736 RX PUBMED; 1917832. RA Dietrichs D., Meyer M., Rieth M., Andreesen J.R.; RT "Interaction of selenoprotein PA and the thioredoxin system, components of RT the NADPH-dependent reduction of glycine in Eubacterium acidaminophilum and RT Clostridium litorale [corrected"; RL J. Bacteriol. 173(19):5983-5991(1991). XX RN [2] RP 1-6736 RX DOI; 10.1111/j.1432-1033.1992.tb16903.x RX PUBMED; 1587286. RA Schrader T., Andreesen J.R.; RT "Purification and characterization of protein PC, a component of glycine RT reductase from Eubacterium acidaminophilum"; RL Eur. J. Biochem. 206(1):79-85(1992). XX RN [3] RP 1-861, 3216-5331 RA Lubbers M.; RT "Eubacterium acidaminophilum selenoprotein B"; RL Thesis (1993), University of Goettingen, Germany XX RN [4] RP 862-3215 RX DOI; 10.1111/j.1432-1033.1993.tb18307.x RX PUBMED; 8223622. RA Lubbers M., Andreesen J.R.; RT "Components of glycine reductase from Eubacterium acidaminophilum. Cloning, RT sequencing and identification of the genes for thioredoxin reductase, RT thioredoxin and selenoprotein PA"; RL Eur. J. Biochem. 217(2):791-798(1993). XX RN [5] RP 862-3215 RA Andreesen J.R.; RT ; RL Submitted (26-NOV-1993) to the EMBL/GenBank/DDBJ databases. RL Jan Remmer Andreesen, Institut fuer Mikrobiologie, Georg-August-Universitat RL Gottigen, D-06099 Halle, Germany XX RN [6] RP 1-6736 RA Andreesen J.R.; RT ; RL Submitted (14-MAY-1996) to the EMBL/GenBank/DDBJ databases. RL Jan Remmer Andreesen, Institut fuer Mikrobiologie, Georg-August-Universitat RL Gottigen, D-06099 Halle, Germany XX RN [7] RP 1-6736 RA Andreesen J.R.; RT ; RL Submitted (24-DEC-1997) to the EMBL/GenBank/DDBJ databases. RL Jan Remmer Andreesen, Institut fuer Mikrobiologie, Georg-August-Universitat RL Gottigen, D-06099 Halle, Germany XX DR CABRI; DSM 3953. XX CC On Dec 24, 1997 this sequence version replaced gi:1353195. XX FH Key Location/Qualifiers FH FT source 1..6736 FT /organism="Eubacterium acidaminophilum" FT /mol_type="genomic DNA" FT /tissue_lib="DSM3953" FT /db_xref="taxon:1731" FT CDS <1..725 FT /codon_start=3 FT /transl_table=11 FT /transl_except=(pos:456..458,aa:OTHER) FT /gene="grdB" FT /product="selenoprotein B of glycine-reductase" FT /note="substrate-binding subunit; selenocysteine" FT /db_xref="GOA:Q9R4G8" FT /db_xref="InterPro:IPR010187" FT /db_xref="UniProtKB/Swiss-Prot:Q9R4G8" FT /protein_id="AAB93302.2" FT /translation="LKGEEYETEYPMPSFDKVEPGKAIKDMSKAKIAIVTSGGIVPKGN FT PDRIESSSASKYGKYDIQGIDDLTSEGWETAHGGHDPIYANEDADRVIPVDVLRDMEKE FT GVIGELHRYFYSTTGNGTAVASSKKFAEEFTKELVADGVDAVILTSTXGTCTRCGASMV FT KEIERSGIPVVHIATVTPISLTVGANRIVPAIAIPHPLGNPALSHEEEKALRRKIVEKA FT LEALQTEVEEQTVFERNY" FT stem_loop 771..819 FT RBS 918..923 FT CDS 932..1879 FT /codon_start=1 FT /transl_table=11 FT /gene="trxB" FT /product="thioredoxin reductase" FT /note="GTG start codon" FT /db_xref="GOA:P50971" FT /db_xref="HSSP:1TDE" FT /db_xref="InterPro:IPR005982" FT /db_xref="UniProtKB/Swiss-Prot:P50971" FT /protein_id="AAB93303.1" FT /translation="MENVYDLAIIGSGPAGLAAALYGARAKMKTIMIEGQKVGGQIVIT FT HEVANYPGSVREATGPSLIERMEEQANEFGAEKVMDKIVDVDLDGKIKVIKGEKAEYKA FT KSVILATGAAPRLAGCPGEQELTGKGVSYCATCDADFFEDMEVFVVGGGDTAVEEAMYL FT AKFARKVTIVHRRDELRAAKSIQEKAFKNPKLDFMWNSAIEEIKGDGIVESAVFKNLVT FT GETTEYFANEEDGTFGIFVFIGYIPKSDVFKGKITLDDAGYIITDDNMKTNVEGVFAAG FT DIRVKSLRQVVTACADGAIAATQAEKYVEANFEE" FT RBS 1950..1955 FT CDS 1965..2297 FT /codon_start=1 FT /transl_table=11 FT /gene="trxA" FT /product="thioredoxin" FT /db_xref="GOA:P21610" FT /db_xref="HSSP:1FAA" FT /db_xref="InterPro:IPR012335" FT /db_xref="UniProtKB/Swiss-Prot:P21610" FT /protein_id="AAB93304.1" FT /translation="MSALLVEIDKDQFQAEVLEAEGYVLVDYFSDGCVPCKALMPDVEE FT LAAKYEGKVAFRKFNTSSARRLAISQKILGLPTITLYKGGQKVEEVTKDDATRENIDAM FT IAKHVG" FT stem_loop 2319..2352 FT RBS 2374..2379 FT CDS 2389..2865 FT /codon_start=1 FT /transl_table=11 FT /transl_except=(pos:2518..2520,aa:OTHER) FT /gene="grdA" FT /product="selenoprotein A of glycine-reductase" FT /note="glycine reductase protein PA; electron-accepting FT protein reduced by the thioredoxin system; selenocysteine" FT /db_xref="GOA:P50972" FT /db_xref="InterPro:IPR006812" FT /db_xref="UniProtKB/Swiss-Prot:P50972" FT /protein_id="AAB93305.2" FT /translation="MSLFDGKKVIIIGDRDGIPGPAIAECLKGTAAEVVYSATECFVXT FT AAGAMDLENQNRVKGFADQFGAENLVVLVGAAEAESAGLAAETVTAGDPTFAGPLAGVQ FT LGLRVFHAVEPEFKDAVDSAVYDEQIGMMEMVLDVDSIIAEMKSIREQFGKFND" FT stem_loop 2933..2965 FT RBS 3133..3137 FT CDS 3147..4688 FT /codon_start=1 FT /transl_table=11 FT /gene="grdC" FT /product="glycine reductase" FT /function="catalyzes reductive conversion of a protein FT A-bound" FT /note="54 kDa subunit of Protein C" FT /db_xref="GOA:P54935" FT /db_xref="InterPro:IPR017236" FT /db_xref="UniProtKB/Swiss-Prot:P54935" FT /protein_id="AAB93306.1" FT /translation="MNFPVLKGAGYVLVHTPDMIMHNGTTQTTEKIVNPESEYLKKLPE FT HLRSFEDVVAYAPNQTYIGSMTPEALGEIAMPWWTEDKKVAGADRYGKLGEIMPQDEFL FT ALMSASDVFDLVLFEKEFIEGAKAKLAAHPVVGNLAESVNAGVELAEIEKQLSEFHAEG FT LYNNGKLVGCVKRAHDVDVNLNSHTMLENLAVKASGVLALANLIAKNNVNPAEVDYIIE FT CSEEACGDMNQRGGGNFAKALAEMTGCVNATGSDMRGFCAGPTHALIAAAALVKSGVYK FT NVIIAAGGATAKLGMNGKDHVKKEMPILEDCLGGFAVLVSENDGVNPILRTDLVGRHTV FT ATGSAPQAVIGSLVLSPLKAGGLKITDVDKYSVEMQNPDITKPAGAGDVPEANYKMIAA FT LAVMGKEIERADIAAFVEKHGMVGWAPTQGHIPSGVPYIGFAISDLTEGSVNRTMIVGK FT GSLFLGRMTNLFDGVSIVAERNTGKVESGSSVSTEEIRKMIAESMKDFAAHLLAE" FT RBS 4687..4694 FT CDS 4699..5856 FT /codon_start=1 FT /transl_table=11 FT /gene="grdD" FT /product="glycine reductase" FT /function="catalyzes reductive conversion of a protein FT A-bound" FT /note="40 kDa subunit of Protein C" FT /db_xref="GOA:Q47878" FT /db_xref="InterPro:IPR012116" FT /db_xref="UniProtKB/Swiss-Prot:Q47878" FT /protein_id="AAB93307.1" FT /translation="MSDIKQMIGKTFMEIADAIETGSFAGKVKVGITTLGSEHGVENLV FT KGAELAAKDAAGFDIVLIGPKVETSLEVVEVATEEEAHKKMEELLDSGYIHSCVTVHYN FT FPIGVSTVGRVVTPGMGKEMFIATTTGTSAAQRVEAMVRNALYGIITAKSMGIENPTVG FT ILNLDGARAVERALKELAGNGYPITFAESLRADGGSVMRGNDLLGGAADVMVTDSLTGN FT IMMKVFSSYTTGGSYEGLGYGYGPGIGDGYNRTILILSRASGVPVAANAIKYAAKLAQN FT NVKAIAAAEFKAAKAAGLESILAGLSKDTKKASTEEEVKMPPKEVVTGTISGVDVMDLE FT DAQKVLWKAGIYAESGMGCTGPIVMVNEAKVEEAAKILKDAGIVA" FT stem_loop 5904..5931 FT RBS 6220..6224 FT CDS 6231..>6736 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="orf1" FT /db_xref="GOA:O53035" FT /db_xref="HSSP:1EP3" FT /db_xref="InterPro:IPR017927" FT /db_xref="UniProtKB/TrEMBL:O53035" FT /protein_id="AAB93308.1" FT /translation="MFKIVEKKVLAPSIFLMKIDAPRVAKSAKPGQFVIIRMDEKGERI FT PLTICDYDKEAGTVTIVVQSMGCSTRRMAEFNEGDYFSDFVGPLGRESEFVHESIDALK FT NKKILFVAGGVGTAPVYPQVKWLHENGIDADVIIGAKNKEMMIMEEELKSVSKNLYPAT FT DDGSS" XX SQ Sequence 6736 BP; 2384 A; 1232 C; 1638 G; 1482 T; 0 other; agcttaaagg cgaagagtat gaaactgagt atccaatgcc ttcatttgac aaggttgagc 60 caggaaaagc tataaaagac atgtctaaag ctaaaatagc tatagtaact tctggtggta 120 tagttccaaa aggaaaccca gacagaatag agtcttcatc agcttctaag tatggcaagt 180 atgacataca aggaatagat gatcttactt cagaaggctg ggagacagct cacggcggac 240 acgacccaat ctacgctaac gaagatgcag acagggttat accagtagac gtgcttagag 300 acatggagaa agaaggcgta ataggcgagc ttcacagata cttctactca acaactggta 360 acggtactgc tgttgcaagc tctaagaaat tcgctgaaga attcactaag gaactagtag 420 cagacggcgt tgatgctgtt atactaactt ctacatgagg cacttgtact agatgcggtg 480 catctatggt taaagaaata gaaagatctg gaataccagt agttcacata gctacagtta 540 ctccaatatc tctaacagtt ggagccaaca gaatagttcc agctatagca atacctcacc 600 cacttggaaa cccagctctt tctcatgaag aggaaaaagc tcttagaaga aagatagttg 660 agaaggctct tgaagccctt caaactgagg ttgaagagca aacggtattc gaaagaaact 720 attaatatca ataaatgcag gcaaggcttt tgccctgcct gcaatatata agtattttca 780 aggtatcttt tattggatta aaagatattt tgaaaatgtc tttgaatagt cacattagag 840 atgaaaacgt caaaggctgg taagatacat aattcataac ggatttttta aaaaatttaa 900 taatgctaag tatcttaagg aggaagtcac agtggaaaat gtatacgatt tggccataat 960 aggttcaggt cctgcagggc tggcagcagc tctatacgga gccagagcca agatgaagac 1020 aatcatgatt gaaggtcaga aagtcggagg acaaatagta ataactcacg aggttgcaaa 1080 ctacccaggc tcagtaagag aagctacggg tccatcactt atagagagaa tggaagagca 1140 ggctaacgag ttcggcgccg agaaggtaat ggacaagata gtagacgttg accttgacgg 1200 caagataaaa gtaataaaag gcgaaaaagc cgagtacaag gcaaaatcag taatacttgc 1260 aacaggagcg gctccaagac ttgcaggctg cccgggagag caggagctta caggcaaggg 1320 agtatcatac tgcgcaactt gcgacgctga cttcttcgag gacatggaag ttttcgtagt 1380 aggcggagga gacactgctg ttgaagaggc tatgtacctt gcgaagtttg caagaaaggt 1440 tacaatagtt cacagaagag acgagctaag agctgccaag tctatacagg agaaggcatt 1500 caagaatcct aagctagact tcatgtggaa ctctgcaata gaagaaataa agggtgacgg 1560 catagttgaa tctgcagtat tcaagaacct tgttactgga gagactacag agtactttgc 1620 aaacgaagaa gacggcacat tcggaatatt cgtattcata ggctacatac ctaagagcga 1680 cgtattcaag ggcaagataa cgcttgacga tgccggatat ataataacag acgacaacat 1740 gaagacaaat gttgaaggcg tatttgcagc aggagacata agagtcaagt ctctaagaca 1800 agtagttact gcatgcgcag acggagctat agctgcaact caggctgaaa aatatgttga 1860 ggctaacttc gaagagtaat attaagcaaa aaaatgttta gtgatttcaa atgatggcaa 1920 taaagttaat aatataataa aactttaaaa ggagatgact tttaatgagc gcattactag 1980 ttgaaataga caaggatcaa ttccaagcag aggtactaga ggcagaaggc tatgtactag 2040 ttgattactt cagcgacggc tgtgtaccat gcaaggctct tatgcctgac gtggaagagc 2100 tggctgctaa atacgaaggc aaggttgcat tccgcaaatt caacacaagc tcagcaagaa 2160 gacttgcaat aagccaaaaa atacttggac ttccaacaat aacactttac aagggtggac 2220 aaaaggtaga ggaagttact aaagacgacg ctactagaga aaacatagat gcaatgatag 2280 ctaagcacgt tggctaatat ttactatctt aattaagtaa gtagactttg gccgtttggc 2340 gggagtttat tttatagatt taaaattttt tataggagga tggtaaccat gagtttgttt 2400 gacggcaaaa aagtcatcat catcggtgac agagacggta taccaggtcc tgctatcgca 2460 gaatgtttaa aaggcactgc agcagaagta gtttactctg ctacagaatg ctttgtctga 2520 acagctgctg gtgcaatgga cctagagaat cagaataggg ttaaaggctt tgctgatcag 2580 tttggagcag agaatcttgt agttcttgtt ggtgcagcag aagctgaatc agcaggacta 2640 gctgctgaaa ctgttacagc aggagaccct acattcgcag gaccacttgc aggagtccag 2700 ttgggactaa gagtattcca cgcagttgag ccagaattca aagatgccgt agactcagca 2760 gtttacgatg agcaaatagg tatgatggaa atggttcttg acgttgactc gataatagct 2820 gaaatgaagt caataagaga gcagtttggt aagttcaacg actaatagca gtatatacca 2880 aaaaataaaa gcatgtgccg gatagtttta tccggcacag cttagcaaaa gtataaatgg 2940 gtgattttcc aaatcaccca tttattaaaa tgcaggaatt ttaacaaaaa aattataatc 3000 aagaaaacta cgataagatt attttataga tttagatata agagagatat atggtggaag 3060 gctgtctgat gacaaatttc tacattatat ataatatgct taataataag caaatatgat 3120 ttcaaaaatt ttggaggtgt caatatatga attttccagt tcttaaaggt gcgggatacg 3180 ttctcgtgca cacaccagac atgataatgc acaacggaac aactcagaca acagaaaaga 3240 ttgtaaaccc agaatcagaa tacctaaaaa agctgcctga gcatttaaga tcatttgaag 3300 acgtagtagc atacgctcca aaccagacat acattggaag catgactcca gaggcacttg 3360 gagaaatagc aatgccatgg tggacagaag acaagaaagt ggcgggagcc gacagatacg 3420 gaaagcttgg agagataatg cctcaggacg agttccttgc gcttatgtct gcatcagacg 3480 tattcgacct tgtgcttttc gaaaaagagt tcatagaggg agcaaaggct aagcttgcag 3540 ctcaccctgt agtaggcaac cttgcagaga gcgtaaacgc aggagtagag ctagctgaga 3600 tagaaaagca gctaagcgag ttccacgcag aaggactata caataacggc aagcttgtag 3660 gctgcgtaaa aagagcccac gacgtagacg tgaacctaaa ctctcataca atgcttgaga 3720 accttgcggt taaggcatca ggagtgcttg ctcttgcaaa ccttatagct aaaaacaacg 3780 taaacccagc tgaagtggac tacatcatag agtgctctga agaggcttgc ggagacatga 3840 accaaagagg aggcggaaac ttcgccaagg ctcttgcaga aatgactggc tgcgttaacg 3900 cgacaggctc agacatgaga ggcttctgcg caggaccaac gcacgcactt atagcggcag 3960 cggcgctagt taaatcaggc gtatacaaga acgtaataat agcagcgggt ggagcgacag 4020 caaagcttgg aatgaacgga aaagaccacg ttaagaaaga aatgccaata cttgaggact 4080 gccttggcgg cttcgcggtt ctagttagcg aaaacgacgg agtaaaccca attctaagaa 4140 cagacctagt gggaagacac acagttgcaa ctggatcagc tccacaggct gtaataggct 4200 ctctagttct aagcccacta aaagcaggcg gacttaaaat aacagacgta gacaagtact 4260 cggtagaaat gcaaaaccca gacataacta agccggcagg agcaggagac gttccagagg 4320 ccaactacaa gatgatagcc gcacttgctg tcatgggaaa agaaatagaa agagcagaca 4380 tagcagcttt cgttgaaaag cacggaatgg ttggttgggc tccaacacaa ggccacatac 4440 catcaggagt accatacatc ggctttgcaa taagcgacct tacagaaggc tcagtaaaca 4500 gaactatgat agtaggaaag ggaagcctct tccttggaag aatgacaaac cttttcgatg 4560 gtgtttcaat agtagcagag agaaacacag gcaaagtgga gtcaggaagc tcagtatcaa 4620 cagaagagat aagaaaaatg atagcagaat ctatgaagga tttcgcagca catctattag 4680 ctgaataggg gtgaaaaaat gtcagatata aaacaaatga taggcaagac tttcatggag 4740 atagcagacg ctatcgaaac aggaagcttc gctggaaaag taaaagtagg aataacaacc 4800 ctgggcagcg agcacggagt agagaacctg gtaaagggag cagagcttgc agctaaagac 4860 gcagccggct ttgacatagt gcttatagga ccaaaggttg aaacaagcct tgaagtagta 4920 gaggtagcaa cagaagaaga agcgcacaag aaaatggaag agcttctaga cagcggctat 4980 atccactctt gcgtaacagt gcactataac ttcccaatag gcgtatcgac agtaggaagg 5040 gtagtaacac ctggaatggg caaggagatg ttcatagcaa caacaacagg aacatctgcg 5100 gctcaaagag tcgaggccat ggtaagaaac gcgctttacg gaataataac agcaaagtca 5160 atgggaatag aaaacccgac agttggaata ctaaaccttg acggagcaag agcagtagaa 5220 agagcgctta aggagcttgc aggaaacggc taccctataa cattcgcaga gtcgctaaga 5280 gctgacggcg gaagcgtaat gagaggaaac gaccttcttg gcggagctgc agacgtaatg 5340 gtaacagact cgctaacagg caacataatg atgaaggtgt tctcgtcata cacaacaggc 5400 ggaagctacg agggactagg ctacggctac ggccctggaa taggcgacgg ctacaacagg 5460 acaatactga tactctcaag agcgtcagga gttccagtag ctgcaaacgc aataaaatac 5520 gcagccaagc ttgcgcaaaa caacgtgaag gcgatagcag cggcagagtt caaggcggcc 5580 aaggcggcag gcctcgagag catacttgcg ggactaagca aggacactaa gaaagcctct 5640 acagaggaag aagtgaagat gcctcctaag gaagtagtaa caggaacaat ctcaggcgta 5700 gacgtaatgg accttgaaga cgcacagaaa gtgctttgga aagccggaat atacgcagag 5760 agcggaatgg gctgcacagg acctatagta atggtaaacg aagccaaggt tgaagaagcg 5820 gcaaagatac ttaaggatgc aggcatagta gcttaatagc tatgcagcca gatcttaaca 5880 ataaataaac aatgtcagaa tatgaaaggc aggaataatt tcctgccttt tttttaatat 5940 aatgaatatt cacaataatc tatacaattt cttgttaaaa tagtgaaaaa attagcaaaa 6000 aatcaatgaa acctagactg acacgctaat gtaagtctgt cttcttaata tggaaaaaat 6060 aacatcaaaa agagctaatc aacttgataa ttaataaata aactgatata atcaatattg 6120 tagagacata aagcaaaagg gctgccagat taaaccaatc tgaaagtcct ttttccggta 6180 gcacggtaaa tgttgtgaat attcaaaaaa ttaaaccatg gaggcgtgaa atgttcaaaa 6240 tagttgaaaa gaaagtactt gcgccatcta tattccttat gaaaatagat gcgccaaggg 6300 tagcaaaatc agctaagcca ggacagtttg taataatcag aatggatgaa aagggtgaaa 6360 gaataccgct gacaatatgc gactatgaca aagaggcggg aacggttaca atagtagttc 6420 agtctatggg atgctcaaca agaagaatgg cagagttcaa cgaaggcgac tacttctcgg 6480 acttcgtagg acctcttgga agagagtctg agtttgttca tgagagcatt gatgcactaa 6540 aaaacaagaa gatactattt gttgcaggcg gagttggaac agctcctgtt tacccgcagg 6600 taaagtggct tcatgaaaat ggaatagatg cagatgtaat aataggtgca aagaacaaag 6660 aaatgatgat aatggaagaa gagctaaaga gcgtgagcaa gaacctttat cctgcaactg 6720 acgacggatc ctctag 6736 // |
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