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EBI DbfetchID L03181; SV 1; linear; genomic DNA; STD; PRO; 4281 BP. XX AC L03181; XX DT 30-SEP-1992 (Rel. 33, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Bacillus subtilis ilvB, ilvN and ilvC genes, complete ilv-leu operon. XX KW attenuator; ilv-leu operon; ilvB gene; ilvC gene; ilvN gene; KW promoter region; ribosome binding site; transcription control. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-4281 RA Vandeyar M.A., Vander Horn P.B., Rafael J.A., Grandoni J.A., Zahler S.A.; RT "The ilv-leu operon of Bacillus subtilis: Sequences of the ilvB, ilvN and RT ilvC genes, and the control of transcription"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..4281 FT /organism="Bacillus subtilis" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT gene 207..650 FT /gene="ilv-leu operon" FT -35_signal 207..212 FT /gene="ilv-leu operon" FT -10_signal 230..235 FT /gene="ilv-leu operon" FT misc_feature 558..575 FT /gene="ilv-leu operon" FT /standard_name="T-box" FT attenuator 578..650 FT /gene="ilv-leu operon" FT gene 711..2441 FT /gene="ilvB" FT RBS 711..715 FT /gene="ilvB" FT CDS 723..2441 FT /codon_start=1 FT /transl_table=11 FT /gene="ilvB" FT /product="acetolactate synthase" FT /db_xref="GOA:P37251" FT /db_xref="HSSP:1N0H" FT /db_xref="InterPro:IPR012000" FT /db_xref="UniProtKB/Swiss-Prot:P37251" FT /protein_id="AAA22546.1" FT /translation="MGTNVQVDSASAECTQTMSGRLMLIESLKKEKVEMIFGYPGGAVL FT PIYDKLYIQVGTYPSRHEQGAIHAAEGYARVSGNRCRHCHVRPGATNLVTGLADAMIDS FT LPLVVFTGQVATSVIGSDAFQEADILGITMPVTKHSYQVRQPEDLPRIIKEAFHIATTG FT RPGPVLIDIPKDVATIEGEFSYDHEMNLPGYQPTTEPNYLQIRKLVEAVSSAKKPVILA FT GAGVLHGKASEELKNYAEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALHE FT CDLLISIGARFDDRVTGNLKHFARNAKIAHIDIDPAEIGKIMKTQIPVVGDSKIVLQEL FT IKQDGKQSDSSEWKKQLAEWKEEYPLWYVDNEEEGFKPQKLIEYIHQFTKGEAIVATDV FT GQHQMWSAQFYPFQKADKWVTSGGLGTMGFGLPAAIGAQLAEKDATVVAVVGDGGFQMT FT LQELDVIRELNLPVKVVILNNACLGMVRQWQEIFYEERYSESKFASQPDFVKLSEAYGI FT KGIRISSEAEAKEKLEEALTSREPVVIDVRVASEEKVFPMVAPGKGLHEMVGVKP" FT gene 2426..2962 FT /gene="ilvN" FT RBS 2426..2430 FT /gene="ilvN" FT CDS 2438..2962 FT /codon_start=1 FT /transl_table=11 FT /gene="ilvN" FT /product="acetolactate synthase small subunit" FT /db_xref="GOA:P37252" FT /db_xref="InterPro:IPR002912" FT /db_xref="UniProtKB/Swiss-Prot:P37252" FT /protein_id="AAA22547.1" FT /translation="MKRIITLTVVNRSGVLNRITGLFTKRHYNIESITVGHTETAGVSR FT ITFVVHVEGENDVEQLTKQLNKQIDVLKVTDITNQSIVQRELALIKVVSAPSTRTEING FT IIEPFRASVVDVSRDSIVVQVTGESNKIEALIELLKPYGIKEIARTGTTAFARGTSKRR FT HPIKQYLLYKT" FT gene 2967..4007 FT /gene="ilvC" FT RBS 2967..2971 FT /gene="ilvC" FT CDS 2979..4007 FT /codon_start=1 FT /transl_table=11 FT /gene="ilvC" FT /product="ketol-acid reductoisomerase" FT /db_xref="GOA:P37253" FT /db_xref="HSSP:1NP3" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P37253" FT /protein_id="AAA22548.1" FT /translation="MVKVYYNGDIKENVLAGKTVAVIGYGSQGHAHALNLKESGVDVIV FT GVRQGKSFTQAQEDGHKVFSVKEAAAQAEIIMVLLPDEQQQKVYEAEIKDELTAGKSLV FT FAHGFNVHFHQIVPPADVDVFLVAPKGPGHLVRRTYEQGAGVPALFAIYQDVTGEARDK FT ALAYAKGIGGARAGVLETTFKEETETDLFGEQAVLCGGLSALVKAGFETLTEAGYQPEL FT AYFECLHELKLIVDLMYEEGLAGMRYSISDTAQWGDFVSGPRVVDAKVKESMKEVLKDI FT QNGTFAKEWIVENQVNRPRFNAINASENEHQIEVVGRKLREMMPFVKQGKKKEAVVSVA FT QN" XX SQ Sequence 4281 BP; 1385 A; 845 C; 1046 G; 1005 T; 0 other; tcaggactca atccctatac gaaccatatc gtgttataaa atcttccatg tttatataaa 60 attaaataat tctggtgtat tcttgataga ttaataaaaa aattataaaa acttttaaca 120 tttgcaattc cttttgtacc aataatgaaa gcgtatacaa tatagattga ttaatcaaaa 180 ttgtctaata attttaaaaa atgctgttga cactgcgtcc aaagcggcgt aatatgagtt 240 caacaaaaga taaatgcaag cttcacaagc gaaaatcatc gcagtatgat tctaaaaaaa 300 tgaaaaacaa acgaccttct tgaacagctg gaaagccgtt ccgaaggctg aatatgaaaa 360 gcgcagatga ggataagtag ccttgataaa gttttccaca gagaaccggg ttagctgaga 420 accggcgaag ctttacaagg tgaactcgcc tcagagtgcc agtctgaaat gacagtagga 480 cttggccggg tgaacttgat tcactcgtta ctaaagcgga tagaaatatc catgagacgg 540 ccgattaaca ggccgtaaac aagggtggta ccgcggaaag aaaagccttt tcgccccttt 600 tagctatcgc agttactgcg cggctgattg tgggcggaag ggcttttttt attgaataat 660 cagctatcta gctaatgaaa agatgatctt taaaggatga aaatccaaaa ggaggaacta 720 aaatggggac taatgtacag gtggattcag catctgccga atgtacacag acgatgagcg 780 gacgattaat gctgattgaa tcattaaaaa aagagaaagt agaaatgatc ttcggttatc 840 cgggcggggc tgtgcttccg atttacgata agctatacat tcaggttggt acatatcctt 900 cccgtcacga acaaggagca attcatgcag cggagggata cgcaagggtc tccggaaacc 960 ggtgtcgtca ttgccacgtc aggccgggag cgacaaacct tgttacaggc cttgctgatg 1020 ccatgattga ttcattgccg ttagtcgtct ttacagggca ggtagcaacc tctgtaatcg 1080 ggagcgatgc atttcaggaa gcagacattt tagggattac gatgccagta acaaaacaca 1140 gctaccaggt tcgccagccg gaagatctgc cgcgcatcat taaagaagcg ttccatattg 1200 caacaactgg aagacccgga cctgtattga ttgatattcc gaaagatgta gcaacaattg 1260 aaggagaatt cagctacgat catgagatga atctcccggg ataccagccg acaacagagc 1320 cgaattattt gcagatccgc aagcttgtgg aagccgtgag cagtgcgaaa aaaccggtga 1380 tcctggcggg tgcgggcgta ctgcacggaa aagcgtcaga agaattaaaa aattatgctg 1440 aacagcagca aatccctgtg gcacacaccc ttttggggct cggaggcttc ccggctgacc 1500 atccgctttt cctagggatg gcgggaatgc acggtactta tacagccaat atggcccttc 1560 atgaatgtga tctattaatc agtatcggcg cccgttttga tgaccgtgtc acaggaaacc 1620 tgaaacactt tgccagaaac gcaaagatag cccacatcga tattgatcca gctgaaatcg 1680 gaaaaatcat gaaaacacag attcctgtag tcggagacag caaaattgtc ctgcaggagc 1740 tgatcaaaca agacggcaaa caaagcgatt caagcgaatg gaaaaaacag ctcgcagaat 1800 ggaaagaaga gtatccgctc tggtatgtag ataatgaaga agaaggtttt aaacctcaga 1860 aattgattga atatattcat caatttacaa aaggagaggc cattgtcgca acggatgtag 1920 gccagcatca aatgtggtca gcgcaatttt atccgttcca aaaagcagat aaatgggtca 1980 cgtcaggcgg acttggaacg atgggattcg gtcttccggc ggcgatcggc gcacagctgg 2040 ccgaaaaaga tgctactgtt gtcgcggttg tcggagacgg cggattccaa atgacgcttc 2100 aagaactcga tgttattcgc gaattaaatc ttccggtcaa ggtagtgatt ttaaataacg 2160 cttgtctcgg aatggtcaga cagtggcagg aaattttcta tgaagaacgt tattcagaat 2220 ctaaattcgc ttctcagcct gacttcgtca aattgtccga agcatacggc attaaaggca 2280 tcagaatttc atcagaagcg gaagcaaagg aaaagctgga agaggcatta acatcaagag 2340 aacctgttgt cattgacgtg cgggttgcca gcgaagaaaa agtattcccg atggtggctc 2400 cggggaaagg gctgcatgaa atggtggggg tgaaaccttg aaaagaatta tcacattgac 2460 tgtggtgaac cgctccgggg tgttaaaccg gatcaccggt ctattcacaa aaaggcatta 2520 caacattgaa agcattacag ttggacacac agaaacagcc ggcgtttcca gaatcacctt 2580 cgtcgttcat gttgaaggtg aaaatgatgt tgaacagtta acgaaacagc tcaacaaaca 2640 gattgatgtg ctgaaagtca cagacatcac aaatcaatcg attgtccaga gggagctggc 2700 cttaatcaag gttgtctccg caccttcaac aagaacagag attaatggaa tcatagaacc 2760 gtttagagcc tctgtcgttg atgtcagcag agacagcatc gttgttcagg tgacaggtga 2820 atctaacaaa attgaagcgc ttattgagtt attaaaacct tatggcatta aagaaatcgc 2880 gagaacaggt acaacggctt ttgcgagggg aaccagcaaa aggcgtcatc caataaaaca 2940 atatctattg tataaaacat aacaagggag agattgaaat ggtaaaagta tattataacg 3000 gtgatatcaa agagaacgta ttggctggaa aaacagtagc ggttatcggg tacggttcgc 3060 aaggccacgc acatgccctg aaccttaaag aaagcggagt agacgtgatc gtcggtgtta 3120 gacaaggaaa atctttcact caagcccaag aagacggaca taaagtattt tcagtaaaag 3180 aagcggcagc ccaagccgaa atcatcatgg ttctgcttcc ggatgagcag cagcaaaaag 3240 tatacgaagc tgaaatcaaa gatgaattga cagcaggaaa atcattagta ttcgctcatg 3300 gatttaacgt gcatttccat caaattgttc ctccggcgga tgtagatgta ttcttagtgg 3360 cccctaaagg cccgggacac ttggtaagaa gaacatatga gcaaggagct ggcgtacctg 3420 cattgttcgc aatctatcaa gatgtgactg gagaagcaag agacaaagcc ctcgcttatg 3480 ctaaaggaat cggcggcgca agagcgggcg tattagaaac gacatttaaa gaagaaacag 3540 aaacagattt gttcggtgag caagcagttc tttgcggcgg attaagcgcg cttgtcaaag 3600 ccggatttga aaccttaact gaagcaggtt atcagcctga acttgcatac ttcgagtgtc 3660 ttcatgagct gaaattaatc gtagacctta tgtacgaaga aggacttgca ggaatgagat 3720 attcaatctc tgacacagca cagtggggag atttcgtatc aggccctcgc gttgtggacg 3780 ctaaagtaaa agaatctatg aaagaagtat taaaagatat ccaaaacggt acattcgcaa 3840 aagagtggat cgtcgaaaac caagtaaacc gtcctcgttt caacgctatc aatgcaagcg 3900 agaacgaaca tcaaatcgaa gtagtgggaa gaaagcttcg tgaaatgatg ccgtttgtga 3960 aacaaggcaa gaagaaggaa gcggtggtct ccgttgcgca aaattaattt tttcgatacg 4020 acgcttcgtg atggtgaaca gtcccctgga gtgaacttga atacacagga gaaacttgcc 4080 atagctaagc agctcgaaag actcggggca gacatcattg aagcgggatt tcccgttcgt 4140 cccgaggtga ctttttagct gttcaggaaa tcgcaagaac cattaaaaat tgttcagtaa 4200 ctggtctggc ccgttgtgta aaaggtgata ttgatgctgc ttgggaagcg ttaaaggatg 4260 gcgctcaacc aagaagaatt c 4281 // |
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