EBI Dbfetch

ID   L02632; SV 1; linear; mRNA; STD; VRT; 2219 BP.
XX
AC   L02632;
XX
DT   03-NOV-1992 (Rel. 33, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 6)
XX
DE   Opsanus tau erythroid 5-aminolevulinate synthase gene, complete cds.
XX
KW   5-aminolevulinate synthase; binding site; erythroid protein;
KW   heme regulatory motif.
XX
OS   Opsanus tau (oyster toadfish)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Euteleostei; Neoteleostei;
OC   Acanthomorpha; Paracanthopterygii; Batrachoididae; Opsanus.
XX
RN   [1]
RP   1-2219
RA   Hellmich H.L., Cornell N.W.;
RT   "5-Aminolevulinate synthase in the marine fish, Opsanus tau";
RL   Unpublished.
XX
CC   On Sep 11, 1994 this sequence version replaced gi:213490.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2219
FT                   /organism="Opsanus tau"
FT                   /mol_type="mRNA"
FT                   /tissue_lib="N.W. Cornell"
FT                   /db_xref="taxon:8068"
FT   protein_bind    3..25
FT                   /function="translational regulator"
FT                   /bound_moiety="iron-responsive element binding protein"
FT                   /note="putative"
FT   stem_loop       3..25
FT   CDS             49..1797
FT                   /codon_start=1
FT                   /product="5-aminolevulinate synthase"
FT                   /function="first reaction in heme biosynthesis"
FT                   /EC_number="2.3.1.37"
FT                   /note="142..156 pentapeptide: RCPVL; putative heme
FT                   regulatory motif; 307..321 pentapeptide: GCPFV; putative
FT                   heme regulatory motif; 67..81 pentapeptide: HCPFL; putative
FT                   heme regulatory motif; erythroid form"
FT                   /db_xref="GOA:P43090"
FT                   /db_xref="InterPro:IPR001917"
FT                   /db_xref="InterPro:IPR004839"
FT                   /db_xref="InterPro:IPR010961"
FT                   /db_xref="InterPro:IPR015421"
FT                   /db_xref="InterPro:IPR015424"
FT                   /db_xref="UniProtKB/Swiss-Prot:P43090"
FT                   /protein_id="AAA60729.1"
FT                   /translation="MAAFLHHCPFLKSMPKPALRRRVPALLSLADRCPVIVHQVCISRL
FT                   HILETKLDVSPTQPKRQRLSLLDQKRLFAQTATQVAVSVSKGCPFVSSQIGMVRASPEV
FT                   QEDVQADLKSPVLPTPPQTGITQLLKDNMVGPSFDYDNFFNEKIAEKKRDHTYRVFKTV
FT                   NRNAVVFPFAEDYSVSDRQGSQVSVWCSNDYLGMSRHPRVLEAIREVLERHGAGAGGTR
FT                   NISGTSKYHVTLEKELAHLHQKDAALVFSSCFVANDSTLFTLAKMLPGCHIYSDAGNHA
FT                   SMIQGIRNSGAKRFIFRHNDSRHLEELLQQSDPKTPKIVAFETVHSMDGAICPLEELCD
FT                   VAHRHGALTFVDEVHAVGLYGAHGAGVGERDNVMHKIDIVSGTLGKAFGCVGGYVASSA
FT                   ALVDTVRSFAAGFIFTTSLPPMILAGALESVRVLKSPEGQLLRRAHQRNVKYMRQLLMD
FT                   KGLPVVNCPSHIIPIRVGNAELNTKVCDSLLEKHNIYVQAINYPTVPRGQELLRLAPSP
FT                   HHHPAMMEYFVDKLVEVWQEAGLLLNGPATVSCTFCDRPLHFDLMSEWEKSYFGNMEPQ
FT                   YITMSA"
FT   polyA_signal    2200..2205
FT   polyA_site      2219
XX
SQ   Sequence 2219 BP; 546 A; 602 C; 557 G; 514 T; 0 other;
     acgttcgtcc tcagtgcagg tcaacagaat agaaacagct ggaccattat ggctgccttc        60
     ctgcaccact gccccttcct gaagtcaatg cctaaaccgg ctttaaggag aagagtacct       120
     gcattactgt ctctggctga ccgatgccct gtcattgttc accaagtctg tattagccgt       180
     ctacacattc tggaaaccaa gctggatgtc tcaccaaccc aacccaaaag acagcgactc       240
     tccttactgg accaaaagag actgtttgct caaacggcca ctcaggtggc cgtgtctgta       300
     tcaaagggct gtcctttcgt cagctctcag attgggatgg tccgagccag ccctgaagtc       360
     caggaagatg tccaagcaga tctgaagagt ccagtcctgc caacaccgcc tcaaaccggc       420
     atcacccaac tcctcaaaga caacatggtt ggccccagct ttgactatga caacttcttc       480
     aacgagaaga ttgcagagaa aaagagggac cacacatacc gagtgtttaa gacagtaaac       540
     cggaacgctg tggtcttccc gtttgctgag gattactccg tctctgaccg acaaggctcc       600
     caggtgtccg tctggtgcag caacgactat ctggggatga gccggcaccc gcgggtcctg       660
     gaggccatca gagaggtcct ggagaggcac ggtgccggag ccggtggaac caggaacatc       720
     tcgggcacca gtaaatacca cgtgactctg gagaaggagt tggctcatct ccaccaaaaa       780
     gatgcagctc tggtcttctc ctcgtgcttt gtggctaatg actccaccct gttcaccctc       840
     gctaagatgc tgcccgggtg tcacatctac tctgatgcgg gaaaccacgc ctcaatgatt       900
     cagggcatca ggaacagcgg cgccaaacgc ttcatcttcc gccacaatga cagccggcac       960
     ctggaggagc tgctgcagca gtcagacccc aaaaccccca aaattgtggc gttcgagacg      1020
     gtgcactcta tggacggtgc catctgtcct ctggaggagc tgtgtgacgt cgctcaccga      1080
     cacggagctc tgacctttgt tgacgaggtt cacgccgtgg gactgtacgg agcccacgga      1140
     gccggcgtgg gagaaagaga caacgttatg cacaagattg acatcgtctc tgggacctta      1200
     ggaaaagctt ttggctgtgt ggggggctac gtcgccagca gcgccgccct ggtggacaca      1260
     gtgcgctcct tcgcagcggg cttcatcttc accacctctt tgcctccgat gatcctggcc      1320
     ggagcgctgg agtccgtgcg ggtgctgaag agtccggagg gacagctgct tcgcagggcc      1380
     caccagagga acgtcaaata catgaggcag ctgctgatgg ataagggcct gccggtggtc      1440
     aactgcccca gccacatcat ccccatccgg gtgggcaacg ccgagctcaa cactaaagtg      1500
     tgtgacagcc tgctggaaaa acacaacatc tacgtccagg ccatcaatta ccccacagtg      1560
     cctcggggcc aggagctgct gcgcctggct ccgtctcctc atcaccaccc tgccatgatg      1620
     gagtacttcg tggacaaact ggtggaggtg tggcaggagg cggggctcct gctgaatgga      1680
     ccggccacgg tctcctgcac cttctgcgac cgcccgctgc actttgacct gatgagcgag      1740
     tgggagaaat cctactttgg caacatggaa ccacaataca ttactatgtc tgcctaaggt      1800
     caaagcacat ccaaacacac agcacgcttt aaaataacaa taataatgat ctgtttgagc      1860
     tcatttctca attttaaaga ttgacttcta atgcgaatgg gttctgatgt gtgcacatct      1920
     tgcttggctt ttaaaaaaac atgattccac atgaatgatc tgcagtaaaa cagtatttac      1980
     tgatgcattt atgtatgtta tgagtctgac ttttaatttg cggccgtgga tataatcagg      2040
     gcagtatttc tggtaaacat gtttaaaatg cttattccaa gtacaaaata gtaatttgta      2100
     tttgacggag gtgttaaaac tgcattgtaa tgaccttgat gttctgtatt tttgcatttt      2160
     aaaactactg aaaatacttt cccccctgtc agtcccttca ttaaaaagtg ggcaattat       2219
//