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EBI DbfetchID L01541; SV 1; linear; genomic DNA; STD; PRO; 2892 BP. XX AC L01541; S52585; XX DT 14-SEP-1992 (Rel. 33, Created) DT 12-FEB-2002 (Rel. 70, Last updated, Version 15) XX DE Bacillus subtilis modification methyltransferase (hsmBI) and restriction DE endonuclease (hsrBI) genes, complete cds. XX KW hsmBI gene; hsrBI gene; modification methyltransferase; KW restriction endonuclease. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2892 RX DOI; 10.1093/nar/20.24.6517 RX PUBMED; 1480472. RA Xu G.L., Kapfer W., Walter J., Trautner T.A.; RT "BsuBI--an isospecific restriction and modification system of PstI: RT characterization of the BsuBI genes and enzymes"; RL Nucleic Acids Res. 20(24):6517-6523(1992). XX CC On Feb 8, 2002 this sequence version replaced gi:263379. XX FH Key Location/Qualifiers FH FT source 1..2892 FT /organism="Bacillus subtilis" FT /strain="ISB8" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT -10_signal 185..190 FT /gene="hsmBI" FT mRNA 198..>1733 FT /gene="hsmBI" FT RBS 214..220 FT /gene="hsmBI" FT CDS 228..1733 FT /codon_start=1 FT /transl_table=11 FT /gene="hsmBI" FT /product="modification methyltransferase" FT /db_xref="GOA:P33563" FT /db_xref="InterPro:IPR002052" FT /db_xref="InterPro:IPR002296" FT /db_xref="UniProtKB/Swiss-Prot:P33563" FT /protein_id="AAA18169.1" FT /translation="MTQILETVDKSRLTVNPLLKNKSELGQFFTPSSISIFMACLFSED FT KLNNAKVLDAGAGIGSLTSAFLARLISENIGKADLHLLEIDEMLEPYLSETLALFKDYI FT EINSQIIIDDFIEWAAYSLLDEESLLAKDKQRFTHAILNPPYKKIKSNSKHRKLLRKAG FT IETVNLYSAFVALTVDLMSDGGEIVFIIPRSFCNGPYFRHFRQHLLNKTSIKHMHLFES FT RDKAFKDDEVLQENVISKLEKGTVQEDVKISISTDDSFSVIRSYRYPFEKIVQPNDIEK FT FIHINTTNEETLIEKHPNVCYSLEELNIEVSTGPVVDFRVKENLREMPGEGTVPLFYPN FT HFVGTSLEYPKMMKKPNAIIRNEKVEKWLYPNGHYVVVKRFSSKEEKRRIVAGVLTPES FT VNDPVVGFENGLNVLHYNKSGISKEVAYGLYAYLNSTPVDKYFRIFNGHTQVNATDLRT FT MKFPSRDILISLGKWVIENIENVGQVEIDSKLEELLLNDRGNA" FT RBS 1697..1701 FT /gene="hsmBI" FT CDS 1714..2664 FT /codon_start=1 FT /transl_table=11 FT /gene="hsrBI" FT /product="restriction endonuclease" FT /db_xref="GOA:P33562" FT /db_xref="InterPro:IPR009528" FT /db_xref="UniProtKB/Swiss-Prot:P33562" FT /protein_id="AAA18170.1" FT /translation="MTEGMHSNVKEAIKILKELGLPKGQQNERSALCLLSLMNITQDKT FT WSEAESPLIGITPMMEFCRINYGKEYAPNSRETFRRFTMHQFVDAGIALYNPDKPTRPV FT NSPKAVYQIEAETLELIKCYNTEEWSELLARYLSNRQTLVERYAKERQQNKIPVQIAEG FT KEIYITPGEHSELIKAIIEEFAPRYVPGGRLIYAGDTGEKMGYFDEELLRQLGVVIDSH FT GKMPDVVIYFPEKKWLLLIESVTSHGPVDHKRHEELAKLFNGSTAGIVYVTAFPNRSLM FT ARYLNNISWETEVWVADAPSHLIHFNGVRFLGPYE" XX SQ Sequence 2892 BP; 1032 A; 419 C; 602 G; 839 T; 0 other; cagctgagaa aaaagaattt ttaaaaatat aggcattgaa gagatacgtg taaaatagaa 60 aagtatatcg aatttaaaag cattgaagaa tgaaaaaaga acatgtctag actatctcta 120 attgtgataa accctgctga aaagtaccag cagggtttat tgtgttaatc attacaaaaa 180 atgataaaat aaaaaagcaa gacgtatcaa tattaaggag tgctaccatg actcaaattc 240 tagaaacagt tgataaatca agattaactg ttaacccatt acttaagaat aagtctgagt 300 tgggtcaatt ttttacacct tcaagtattt ctatatttat ggcttgcttg tttagtgaag 360 ataaattaaa taatgccaaa gttttggatg cgggagcagg aataggatct ctaactagtg 420 ccttcttggc tcgattaatt tcagaaaaca ttggtaaagc tgatcttcat ttgttggaaa 480 tcgatgaaat gttagagcct tatttgtctg aaaccctggc tttattcaaa gattatattg 540 aaattaattc acaaattatt attgatgatt ttattgagtg ggcagcgtat agtttactgg 600 atgaagagag tttactcgca aaagataagc agcggtttac tcatgctatc ttaaatccgc 660 catataagaa aataaaaagc aattctaaac atagaaaact tttaagaaaa gcaggaattg 720 aaactgtaaa cttatattca gcatttgtag ctctgacagt tgatttaatg tctgatggtg 780 gtgaaatagt ttttattata ccaaggagct tctgtaatgg cccttatttt cgtcatttta 840 gacaacactt attgaacaaa acctcaatta aacatatgca tttgtttgaa tctagggaca 900 aggcatttaa ggatgatgaa gttttacaag aaaatgtcat aagtaagcta gaaaaaggaa 960 ctgtgcaaga agatgttaaa atatctatct caacagatga tagcttttct gttataagga 1020 gttataggta tccattcgag aaaattgtcc aaccaaatga tattgaaaag tttattcata 1080 taaacacaac caatgaggaa actcttatag agaagcatcc aaatgtttgt tattccttgg 1140 aggaactaaa tattgaggtt tcaacaggac cagttgtaga tttcagagta aaagaaaatc 1200 taagagaaat gccaggagaa ggaactgttc cattgtttta tccaaatcac tttgttggga 1260 caagtttaga gtaccccaag atgatgaaaa agcctaatgc aatcattaga aatgaaaagg 1320 ttgaaaagtg gctttaccct aatgggcatt atgttgttgt taaaaggttt tcttcaaaag 1380 aagaaaaacg tcgaatagta gcaggagtat taacaccaga gagtgtaaac gatccagtag 1440 ttggttttga gaacggactc aatgtacttc actataataa gagtggaatt tctaaagaag 1500 tagcgtatgg tctttatgcc tatcttaact cgaccccagt agataagtat ttcagaattt 1560 ttaatggtca tactcaggta aatgctactg accttagaac aatgaaattt cctagtagag 1620 atatcctaat aagtttaggt aaatgggtaa tagaaaatat agaaaatgta gggcaggttg 1680 aaatagatag taagttggag gagttgttat taaatgaccg agggaatgca tagtaacgtc 1740 aaagaagcaa taaaaattct taaagaatta ggtttgccta aggggcagca aaatgaacgt 1800 tcagctctat gtttattatc tttaatgaat ataactcaag ataagacatg gtcagaagct 1860 gaaagtcctc ttattggtat aacaccaatg atggaatttt gccgaattaa ttatggaaaa 1920 gaatatgcac caaatagtcg agaaactttt agaaggttca ctatgcatca gtttgtagat 1980 gctggaattg ctttgtataa ccctgataaa ccgacaagac ctgttaatag tccgaaggca 2040 gtatatcaaa tagaagcgga aactttagaa cttattaagt gctataatac agaagaatgg 2100 agtgaattac tagcccgata cttatctaat agacaaacat tggtagaaag gtatgctaaa 2160 gagagacagc agaataaaat acctgtacaa atagctgaag gtaaagagat ttatataact 2220 ccaggagagc acagcgagtt aattaaggca attatagaag aatttgcacc ccgttatgta 2280 ccaggaggca gattaattta tgctggtgat actggtgaaa agatgggtta ttttgatgaa 2340 gaattattaa gacagttagg ggtagttata gactcgcatg gaaaaatgcc tgatgtagta 2400 atttattttc cagagaaaaa gtggctccta ctcattgaat cggtaactag tcacggtcca 2460 gtagaccata agcgtcatga agaattagcc aaattattta atggttcaac tgcgggaata 2520 gtttatgtga ctgcattccc taatcgttca ttaatggcaa gatacctaaa taacatttct 2580 tgggagacgg aagtgtgggt agctgatgca ccgtcacatc taattcattt taatggcgtt 2640 cgtttcttag gcccatacga gtaattttaa tttgactctc ttataaataa ttggaaaaaa 2700 gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga 2760 agcgagcagg actggcggcg gccaaagcgg tcggacagtg ctcgagaacg ggtgcgcata 2820 gaaattgcat caagcatata ggcctagacg cagcccatag tgactggcga tgctgtcgga 2880 atggacgata tc 2892 // |
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