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EBI DbfetchID L01113; SV 1; linear; genomic DNA; STD; PRO; 7339 BP. XX AC L01113; XX DT 02-OCT-1993 (Rel. 37, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 9) XX DE Thiocystis violacea beta-ketothiolase and PHA synthase (phbA and phbC) DE genes, complete cds. XX KW beta-ketothiolase; PHA-biosynthetic gene; PHA-synthase; phbA gene; KW phbC gene. XX OS Thiocystis violacea OC Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; OC Thiocystis. XX RN [1] RP 1-7339 RX DOI; 10.1007/BF00242944 RX PUBMED; 7763384. RA Liebergesell M., Steinbuchel A.; RT "Cloning and molecular analysis of the poly(3-hydroxybutyric acid) RT biosynthetic genes of Thiocystis violacea"; RL Appl. Microbiol. Biotechnol. 38(4):493-501(1993). XX DR RFAM; RF00059; TPP. XX CC On Jun 26, 1996 this sequence version replaced gi:310998. XX FH Key Location/Qualifiers FH FT source 1..7339 FT /organism="Thiocystis violacea" FT /strain="2311" FT /mol_type="genomic DNA" FT /db_xref="taxon:13725" FT CDS complement(<1..414) FT /codon_start=1 FT /transl_table=11 FT /standard_name="ORF4" FT /product="unknown protein" FT /db_xref="InterPro:IPR007897" FT /db_xref="UniProtKB/Swiss-Prot:P45368" FT /protein_id="AAB02859.1" FT /translation="MNSDRIIKKYPNRRLYDTEVSRYITLADVRNLVMDCTSFKVVDTA FT NESDITRSILLQIMLEEETGGEPLFSASMLAQIIRFYGGTLQGMFARYLESSLDLFAKQ FT QQDMTKTLGDNPFEAMTRMTQKNVEIWADMQEEF" FT RBS complement(420..423) FT /standard_name="Shine-Dalgarno sequence" FT terminator complement(470..541) FT gene complement(548..1732) FT /gene="phbA" FT CDS complement(548..1732) FT /codon_start=1 FT /transl_table=11 FT /gene="phbA" FT /product="beta-ketothiolase" FT /EC_number="2.3.1.9" FT /db_xref="GOA:P45363" FT /db_xref="HSSP:1M4T" FT /db_xref="InterPro:IPR016038" FT /db_xref="UniProtKB/Swiss-Prot:P45363" FT /protein_id="AAB02860.1" FT /translation="MSDTIVIVDAGRTAIGTFGGALSALQATDIGTTVLKALIERTGIA FT PEQVSEVILGQVLTAGCGQNPARQTTLMAGLPHTVPAMTINKVCGSGLKAVHLAMQAVA FT CGDAEIVIAGGQESMSQSSHVLPRSREGQRMGDWPMKDTMIVDGLWDAFNQCHMGVTAE FT NIAKKYAFTREAQDAFAAASQQKAEAAIQSGRFADEIIPVSIPQRKGDPLVFDTDEFPR FT PGTTAETLGRLRPAFDKQGTVTAGNASGINDGAAMVVVMKESKAKELGLTPMARLVAFS FT SAGVDPAIMGTGPIPASTDCLKKAGWAPADLDLVEANEAFAAQAMSVNQEMGWDLSKVN FT VNGGAIAIGHPIGASGARVLVTLLYEMQKRDAKKGLATLCIGGGQGVALAVERL" FT RBS complement(1743..1746) FT /standard_name="Shine-Dalgarno sequence" FT -10_signal complement(1794..1799) FT -35_signal 1807..1812 FT -35_signal complement(1817..1822) FT -10_signal 1833..1838 FT RBS 1857..1862 FT /standard_name="Shine-Dalgarno sequence" FT CDS 1870..2964 FT /codon_start=1 FT /transl_table=11 FT /standard_name="ORF2" FT /db_xref="InterPro:IPR010123" FT /db_xref="UniProtKB/Swiss-Prot:P45367" FT /protein_id="AAB02861.1" FT /translation="MSNDSFFNNDWLELQRKYWDSWSEMGRKAMGLENQQTLTTPWEGA FT LDHWWKAMSPATPDFSKTFMEKMMEQGKNFFRMAETFANTPEDTTATNGLTWWTKALED FT MQKQFSGSLDDGGNSMQRMMSFWELPIDNWQRMMSSMSPMPGDMLRNMPHEQLKDRSGR FT ALSAPGLGYTREEQSQYHQLTRTAMDYQAALQEYTGFYSQLGMKSVERMGDFIQGVIDS FT GKSIDSARTLYDNWISCCETVYAAEVATPEYAQIHGRLVNAQMALKRRMAIMVDENLGA FT MNMPTRSELRTLQDRLQETRRDNKQLHRALHALEKQVAALSGKTPTTALKAPAPATKAT FT EKPATRATTRRKTAAKPTGGTADD" FT RBS 3015..3018 FT /standard_name="Shine-Dalgarno sequence" FT gene 3028..4095 FT /gene="phbC" FT CDS 3028..4095 FT /codon_start=1 FT /transl_table=11 FT /gene="phbC" FT /product="PHA synthase" FT /db_xref="GOA:P45366" FT /db_xref="InterPro:IPR010125" FT /db_xref="UniProtKB/Swiss-Prot:P45366" FT /protein_id="AAB02862.1" FT /translation="MRPIDIRPDKLAQEMLDYTSKLGQGMENLLNADAIDTGVSPKEAV FT YSEDKLVLYRYDTPAGVTPQKTPLLIVYALVNRPYMTDIQEDRSTIKGLLATGQDVYLI FT DWGYPDQADSALTLDDYINGYIDSCVDYLCETHEVDQVNILGICQGGAFSLMYASLHPD FT KVKNLVTMVTPVDFKTPGNLLSAWVQNVDIDLAVDTMGNIPGELLNWTFLSLKPFSLTG FT QKYVNMVDMLDDPDKVKNFLRMEKWIFDSPDQAGETFRQFTKDFYQKNGFINGGVKLGG FT KEIDLKNVDCPVLNIYALQDHLVPPDASKALNPWSAARTYTELAFPGGHIGIYVSGKAQ FT KEVTPAIGKWLNERS" FT terminator 4094..4262 FT CDS complement(4765..6843) FT /codon_start=1 FT /transl_table=11 FT /standard_name="OFR5" FT /db_xref="GOA:P45365" FT /db_xref="HSSP:1MB3" FT /db_xref="InterPro:IPR001789" FT /db_xref="UniProtKB/Swiss-Prot:P45365" FT /protein_id="AAB02863.1" FT /translation="MSNAATGSRVLYLGSDQGLMRDLTALVEPKSVQLVQLGSASALAQ FT ELRRLAPEVVILDAAAVSVEGGLAAFLGTLYGGEAFHPRAICIARAGSGEDRMEQRLAA FT LRAGAASYLVPPISVKRLASRVLRMCGIVETVRYRILILEQDASHAKKIASLLATIGME FT TLVVDDPMKILARMQAFRPNLVLMDLYLPGATGSELTTIIRDHDDFYGIPILFLSQEAD FT LDKQLAALKAGGDGFIKKPVSREALIAAVEYRMRMSRWLQDRRTLVNRRETAGGFLPRD FT VFMRHLEQITRAREAQGGVHGLVIIDVDGSQGILNALGLTATEKLLRELESLLSKTMTP FT EESATRMDDFRYGLLAKRETLSKLEALASTLCQQLSGLKPQDRDIKLEVSVSVGVGLFD FT PPADDAFAMVSRAEKAAAGAKSAGGNQAHVWSAASRQNGAPEAESVIKRLVSTALAQDG FT FLLFFQPILSLNQQEDELYEAQIRMKTLDGEQMPPAEFLSVAERAEMMPRIDRWVLRRA FT FEVMHAERVAHPRLRLLVHQNVMTLAAPEWFPWFRDQIIKRNLTQIYPVLELQMADVRQ FT NRAEAKVFIERLRKYGIQVCVANVTGIREEISLLARIGATLAKLAFQTIRNADRIQLTE FT IVQALQARGVAVIAAGIDDQATVSRVWTCRPDFIQGNYLQLPQPELSFDFQHMSHDG" FT RBS complement(6854..6857) FT /standard_name="Shine-Dalgarno sequence" XX SQ Sequence 7339 BP; 1420 A; 2438 C; 2236 G; 1245 T; 0 other; gaattcctcc tgcatatccg cccagatctc aacgttcttt tgcgtcatcc gcgtcatggc 60 ctcgaagggg ttgtcgccaa gcgtcttggt catatcctgc tgctgcttgg cgaagagatc 120 gagcgaggac tccagatagc gagcgaacat cccttgcagg gttccgccgt agaagcgaat 180 gatttgggcg agcatcgagg cactgaagag cggctctcca ccggtttcct cttcaagcat 240 gatctggagc aggatggagc gggtgatatc gctttcattg gccgtgtcga cgaccttgaa 300 gctggtgcaa tccatcacga ggttgcggac gtccgcgagg gtgatgtaac ggctcacctc 360 tgtgtcatag aggcggcgat tggggtattt cttgatgatg cggtcgctgt tcattcagca 420 cctgagctac cgacgttgtc gtccggccag tgtacccgac actcgccgcc gcgcctcgcc 480 gacttcgctc ggcgaggcgc gacctgaccc aaggatccgg gcgggctccg cgcctcgccc 540 gagggcctca gagacgctcg accgccaacg ccacgccctg gccgccgccg atgcagaggg 600 ttgcgagacc cttcttcgca tcgcgcttct gcatctcata gaggagggtg accagcacac 660 gcgcaccaga ggcaccgatc gggtggccga tggcgatagc tccgccattg acgtttacct 720 tgctgagatc ccagcccatc tcctgattga cggacatcgc ctgagcggca aaggcctcat 780 tggcctcgac aaggtccaga tcagccgggg cccagccggc cttcttcaga caatcggtcg 840 aagccgggat aggtccggtg cccatgatgg ccggatccac gcccgcgctc gagaacgcga 900 ccaggcgcgc catgggcgtc aaacccagtt ccttggcctt ggattccttc atcaccacga 960 ccatggccgc gccgtcgttg atcccggagg cgttgccggc ggtcacagtg ccttgcttgt 1020 cgaaggcggg gcgcagcctg ccgagcgttt cggccgtggt gcccggacgc ggaaactcgt 1080 cggtgtcgaa caccagggga tcgcccttgc gctgcgggat gctcaccggg atgatctcgt 1140 cggcaaagcg tccggattgg atggcggcct ccgccttctg ctgggacgcc gcggcgaacg 1200 catcctgcgc ctcgcgggtg aaggcgtact tcttcgcgat gttctccgcc gtgacaccca 1260 tgtggcactg attgaaggca tcccagaggc cgtcgacgat catggtgtcc ttcatcggcc 1320 agtcgcccat gcgctgcccc tcgcgcgagc gcggcaggac gtgcgacgac tgactcatgc 1380 tctcttgacc accggcgatc acgatctccg catcgccaca ggcaaccgcc tgcatggcca 1440 gatggaccgc cttgagccca ctgccgcaga ccttgttgat ggtcatcgcc gggaccgtgt 1500 gcggcagccc cgccatcaag gtggtctgac gcgccggatt ctgaccgcag cccgcggtca 1560 gcacctgccc cagaataact tcgctgacct gctcgggggc gattccggtc cgctcgatca 1620 gggccttgag cacggtcgtg ccgatatcgg tcgcctggag ggccgagagt gcgccgccga 1680 aggttccgat cgcggtccga cccgcatcga cgataacgat agtgtcgctc atggtgtgga 1740 tgtcctctct tagacgatgg aatcggcctt tgcacagggc ttgttgtgtg cggattctac 1800 gcacctttgt tgcagtgcac aaatccgcgt gctaactttt gccaaatatc atcccgagga 1860 ggcaaatgca tgagcaacga tagctttttc aacaacgact ggctggaact tcaacgcaag 1920 tactgggaca gctggtccga gatgggccgc aaggcgatgg ggctcgagaa ccagcagacg 1980 ctgaccacgc cctgggaggg cgcgctcgac cattggtgga aggccatgtc accggccacg 2040 cccgacttct ccaagacctt catggaaaag atgatggagc agggcaagaa cttcttccgc 2100 atggcggaga ccttcgccaa cacacccgag gatacgaccg ccaccaatgg tctcacctgg 2160 tggaccaagg ccctcgagga catgcagaag cagttcagcg gctcgctcga cgacggcggc 2220 aacagcatgc aacgcatgat gtccttctgg gaactgccga tcgacaactg gcagcgcatg 2280 atgtcctcca tgtcccccat gccgggcgac atgctgcgca acatgccgca cgagcagctc 2340 aaggaccgtt ccggccgcgc cctctccgcc cctggcctgg gctacacgcg cgaggagcag 2400 agccagtacc accagctgac gcgcaccgcc atggactatc aggcggcctt gcaggagtac 2460 accggcttct acagccagct gggcatgaag tcggtcgagc gcatgggcga cttcattcaa 2520 ggcgtgatcg acagcggcaa gagcatcgac tcggcacgca ccctctatga caactggatc 2580 agctgctgcg agaccgtcta cgccgccgag gtcgcgaccc cggagtacgc ccagatccac 2640 gggcgcctgg tcaacgcgca gatggcgctg aaacggcgca tggccatcat ggtggacgaa 2700 aacctcggcg ccatgaacat gcccacccgc agcgagctgc gcaccttgca ggaccgactc 2760 caagagacgc gccgcgacaa caaacaactc caccgcgccc tccatgcgct ggaaaagcag 2820 gtcgccgcac tgagcggcaa gaccccaacg accgcactca aggcaccggc acccgccacc 2880 aaagcgaccg agaagccggc gacacgcgcg accaccaggc gcaagaccgc cgccaagccg 2940 accggcggca ccgcggatga ctgagcgcct tccgacgcct aaaaccatgt gcagcgccac 3000 gaaaagacta ctgcgaggat agagaacatg cggcccatcg acattcgccc ggacaagctc 3060 gcgcaggaaa tgctcgacta caccagcaaa ctgggacagg gcatggagaa cctgctcaat 3120 gcggacgcca tcgacaccgg agtcagcccc aaggaggccg tctacagcga ggacaagctc 3180 gtcctctacc gctacgacac cccggccggc gtcacccccc agaagacgcc gctcctgatc 3240 gtctacgccc tggtgaaccg gccctatatg accgacatcc aggaagaccg ctccaccatc 3300 aagggcctgc tcgcaaccgg ccaggatgtc tatctgatcg actggggcta tccggaccag 3360 gccgatagcg ccctcacgct ggacgactac atcaacggct acatcgacag ctgcgtcgac 3420 tatctttgcg agacccatga agtcgaccag gtgaacatcc tcggcatctg ccagggcggc 3480 gccttcagcc tcatgtatgc ctccctgcac ccggacaagg tcaagaacct ggtcaccatg 3540 gtcacccccg tggacttcaa gaccccgggc aatctgctgt cggcctgggt gcagaacgtc 3600 gacatcgact tggccgtgga caccatgggc aacatccccg gcgagttgct gaactggacc 3660 ttcctctcgc tcaaaccctt cagcctcacc ggccagaagt acgtcaacat ggttgacatg 3720 ctggacgacc cggacaaggt caagaacttc ctgcgcatgg aaaagtggat cttcgacagc 3780 cctgaccagg caggcgaaac cttccgccag ttcaccaagg acttctacca gaagaacggc 3840 ttcatcaacg gcggcgtcaa gcttggcgga aaagagatcg acctcaagaa cgtcgactgc 3900 ccggtgctga acatctacgc cctgcaggac cacctggtgc caccggacgc ctccaaggcc 3960 ctcaacccct ggtcggcagc aaggacttat acggagctgg cgttccccgg cggccacatc 4020 ggcatctacg tcagcggcaa ggcccagaaa gaagtcacgc cggcgatcgg caagtggctg 4080 aacgagcggt cgtgatctag cggccagcgg ccagcttgat ggcggctggc cgctggaacg 4140 ctagtcctgg atgtagaaat gacgccaccc ggtcccagtc gctcggatca gcagatcgaa 4200 gagcgcgggg gtcggcgtgg cttcgtccgg aaagatctct tcgatctgaa tgaagaggcg 4260 gccgaagccg gcctgttgct tccagtccca cagccacggc ggccgagtct cgcggcacgt 4320 cggacaggtc acccccgcgt gcggatgcga ggcccagtga gcccgacgct cgcgccaatc 4380 aagtaagcgc gcacggcaac cgacgcagcg tggcgcacgg gtgttgcggc ccgcgagcag 4440 gtagggatgc tctcgcaccg gcggaacctg gatatggcaa aagggcgccc ccgcatccgg 4500 ctccgagcga atcctgaccg cgcatccggc aaaggtcagc aagctgagaa agcctggccc 4560 aatggcgtag gctgctacct ccccctgcgc cagacgctca ccgatgaacc ccttttccgc 4620 cagcatctcg accagcagca ccctttgggg gctggcatga ggatcgagcg tggtgagcac 4680 cagccggccc gtgtgattgg gagcgacgcg gcgggcgaga tccagcccgc ccgaggtcgc 4740 ggacacttcg gcagcaacgg gggctcagcc gtcatgggac atgtgctgga aatcgaagct 4800 cagctcgggc tgaggcagct gcagatagtt gccttgtatg aagtccgggc ggcaggtcca 4860 gacacgcgac accgtggcct ggtcgtcgat cccggccgcg atgaccgcca cacccctcgc 4920 ctgcagggcc tgaacgatct cggtcagctg gatccgatcc gcgttgcgaa tcgtctggaa 4980 ggcaagtttc gcgagggtcg cgccgatgcg cgcaagcaag gagatctctt cccggatgcc 5040 ggtgacattg gcgacacaga cctggatccc gtatttgcgc agacgctcga tgaacacctt 5100 tgcctcggcg cggttctgtc ggacatcggc catctgcagc tcgagcacgg ggtagatctg 5160 cgtcagattg cgtttgatga tctggtccct gaaccagggg aaccactcgg gcgccgccag 5220 cgtcatgacg ttctggtgga ccaggaggcg caaacgtgga tgcgcgacgc gttcggcatg 5280 catgacctcg aaggcgcgcc tgagcaccca gcggtcgatc cttggcatca tctcggcgcg 5340 ctcggcgacg gacaggaatt ccgcgggtgg catctgctcg ccatccagcg tcttcatgcg 5400 aatctgcgcc tcgtagagct cgtcctcctg ctgattgagc gagaggatcg gctggaagaa 5460 cagcagaaag ccgtcttgcg ccaacgcggt gctcaccaga cgcttgatga ccgactccgc 5520 ttccggggca ccattctgcc ggctggccgc agaccagacg tgcgcctggt ttccgcccgc 5580 cgatttggcg cccgcggcgg ccttttccgc gcgcgacacc atggcgaagg cgtcgtcggc 5640 cggcgggtcg aagagaccga cgccgacgct gacgctgacc tccaacttga tgtctctgtc 5700 ctgcggcttg aggccggaga gctgttgaca gagggtgctg gcgagcgcct cgagcttgga 5760 cagggtctcc cgcttggcca gcagaccgta gcggaaatcg tccatgcggg tcgcgctctc 5820 ctcgggtgtc atcgtcttgc tgagcagcga ctccagctca cgcagcaact tctcggtcgc 5880 cgtcaaaccc aaggcgttca ggatgccctg cgagccgtcg acgtcgatga tcaccagccc 5940 atgaacaccg ccctgagcct cgcgggcacg cgtgatctgc tcgaggtggc gcatgaagac 6000 gtcgcgcgga aggaagccgc cggccgtctc gcgccgattg accagggtgc ggcggtcctg 6060 cagccagcgc gacatgcgca tgcggtattc gacggcggcg atgagcgcct cccggctcac 6120 cggcttcttg atgaaaccgt cgccgcccgc cttgagcgcc gccagctgct tgtcgaggtc 6180 ggcttcctgg gacaggaaga ggatcggaat cccgtagaag tcgtcgtgat cgcgaatgat 6240 cgtggtcagc tccgaccccg tcgccccggg caaatagaga tccatcagca ccaggttcgg 6300 gcggaaggcc tgcatacgcg ccaggatctt catggggtca tcgaccacca aggtctccat 6360 cccgatcgtc gcgagcagcg aggcgatctt cttcgcgtgc gacgcatcct gctcaagaat 6420 caggatacgg tagcgcaccg tctcgacgat gccacacatc ctgaggacgc ggctcgccaa 6480 tcgcttcacg gaaatgggcg ggaccagata gctggcggcc ccggcgcgca gggcggcaag 6540 ccgttgctcc atgcggtctt cgccgctgcc tgcgcgcgcg atgcagatcg cacgcggatg 6600 gaacgcctcg ccaccgtaca gcgtacccag gaacgccgcg agcccgccct ccaccgagac 6660 cgccgccgca tcgaggatga cgacctctgg cgccaggcgg cgcagctcct gagcgagcgc 6720 ggacgcactg ccgagttgaa cgagctggac ggatttcggc tcaacgagcg ccgtcagatc 6780 cctcatcaac ccctgatctg atccgagata aaggacgcga ctgcccgttg ctgcattgct 6840 cataggttcc atgtcctgtt aagcctggtc tggttcggaa cgtcgcgcgc ggtcgttgtg 6900 aaacgcccgc tccggtctcg taattctgtc acgccgggtt ttctattgaa atcgccgcgt 6960 cctttcgcta gcttcgagcc tagccatact gctgggggtg tccgtgacga cccgctcggc 7020 ttggattttc ggcgctgggc gctcagacgt ccagccgcta tccctgccga gagtttcgcg 7080 tctcgggctg agaaaagacc cttcgaacct gatccggatc atgctggcgc aggaaagcag 7140 gcaagacaga ggccatgccg tcgcgcaacc gctcgtcgga aagcccgcgg cattcgccgt 7200 cgtcccacct gcccccatcc gcccgcgccc gccaggccat cgaggcgccc cgcagccgta 7260 tccaagctga gtccatcagg cgcgtgcagc gcccccaaca gcctccagga gcacgatgag 7320 cgccatccca cacgaattc 7339 // |
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