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EBI DbfetchID L01112; SV 1; linear; genomic DNA; STD; PRO; 6977 BP. XX AC L01112; XX DT 17-NOV-1992 (Rel. 34, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 7) XX DE Chromatium vinosum poly(3-hydroxybutyric acid) (phbA, phbB, phbC) genes, DE complete cds. XX KW phbA gene; phbB gene; phbC gene; poly(3-hydroxybutyric acid). XX OS Allochromatium vinosum OC Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; OC Allochromatium. XX RN [1] RP 1-6977 RX DOI; 10.1111/j.1432-1033.1992.tb17270.x RX PUBMED; 1396692. RA Liebergesell M., Steinbuechel A.; RT "Cloning and nucleotide sequences of genes relevant for biosynthesis of RT poly(3-hydroxybutyric acid) in Chromatium vinosum strain D"; RL Eur. J. Biochem. 209(1):135-150(1992). XX FH Key Location/Qualifiers FH FT source 1..6977 FT /organism="Allochromatium vinosum" FT /strain="D" FT /mol_type="genomic DNA" FT /db_xref="taxon:1049" FT terminator complement(777..847) FT /gene="phbC" FT CDS complement(832..1899) FT /codon_start=1 FT /transl_table=11 FT /gene="phbC" FT /product="3-hydroxybutyric acid" FT /EC_number="1.1.1.35" FT /EC_number="2.3.1.9" FT /db_xref="GOA:P45370" FT /db_xref="InterPro:IPR010125" FT /db_xref="UniProtKB/Swiss-Prot:P45370" FT /protein_id="AAA23320.1" FT /translation="MFPIDIRPDKLTQEMLDYSRKLGQGMENLLNAEAIDTGVSPKQAV FT YSEDKLVLYRYDRPEGAPEAQPVPLLIVYALVNRPYMTDIQEDRSTIKGLLATGQDVYL FT IDWGYPDQGDRALTLDDYINGYIDRCVDYLREAHGVDKVNLLGICQGGAFSLMYSALHP FT DKVRNLVTMVTPVDFKTPDNLLSAWVQNVDIDLAVDTMGNIPGELLNWTFLSLKPFSLT FT GQKYVNMVDLLDDPDKVKNFLRMEKWIFDSPDQAGETFRQFIKDFYQNNGFLNGGVVLG FT GQEVDLKDITCPVLNIFALQDHLVPPDASRALKGLTSSPDYTELAFPGGHIGIYVSGKA FT QKEVTPAIGKWLNER" FT RBS complement(1908..1912) FT /gene="phbC" FT mRNA complement(<1957..3064) FT /gene="phbC" FT CDS complement(1957..3030) FT /codon_start=1 FT /transl_table=11 FT /gene="phbC" FT /note="ORF2" FT /db_xref="InterPro:IPR010123" FT /db_xref="UniProtKB/Swiss-Prot:P45372" FT /protein_id="AAA23321.1" FT /translation="MSNTNFFNDDWLELQRKYWDNWTDMSRKAMGLDSASSSANTPWEA FT AIDQWWKAMAPAAPDLSRSFMEKMMEQGKNFFRLADTFAKRADEGNAGNGLELWTKTLE FT DMQKRFSGSLDDGGNTMQRLMSFWELPLDNWQRMMSSMSPMPGDMLRNMPHEQFKDSLD FT RALSAPGLGYTREEQSQYQELMRSAMEYQAALQEYTNVYTKLGMKSVEHMGSYIQGVID FT SGKTIDSARALYDNWVACCEGAYADEVATPEYARIHGRLVNAQMALKKRMSILVDENLG FT ALNMPTRSELRTLQDRLQETRRENKALRHSLRDLERRVAALAGEEPATKPATALRSPAP FT AAKAPARRRTTKTNPAD" FT RBS complement(3040..3045) FT /gene="phbC" FT /note="for ORF2" FT -10_signal complement(3072..3077) FT /gene="phbC" FT -35_signal 3083..3088 FT /gene="phbA" FT -35_signal complement(3093..3098) FT /gene="phbA" FT -10_signal 3106..3111 FT /gene="phbA" FT mRNA 3118..>4365 FT /gene="phbA" FT RBS 3166..3170 FT /gene="phbA" FT CDS 3181..4365 FT /codon_start=1 FT /transl_table=11 FT /gene="phbA" FT /product="3-hydroxybutyric acid" FT /EC_number="1.1.1.35" FT /EC_number="2.3.1.9" FT /db_xref="GOA:P45369" FT /db_xref="HSSP:1M4T" FT /db_xref="InterPro:IPR002155" FT /db_xref="UniProtKB/Swiss-Prot:P45369" FT /protein_id="AAA23322.1" FT /translation="MSENIVIVDAGRSAIGTFGGSLSSLSATEIGTAVLKGLLARTGLA FT PEQIDEVILGQVLTAGVGQNPARQTTLHAGLPHSVPAMTINKVCGSGLKAVHLAMQAIA FT CGDADIVIAGGQESMSQSSHVLPRSRDGQRMGDWSMKDTMIVDGLWDAFNNYHMGTTAE FT NIAQKYGFTREQQDAFAAASQQKTEAAQKAGRFQDEIIPIEIPQRKGDPKVFDADEFPR FT HGTTAESLGKLRPAFSKDGSVTAGNASGINDGAAMVVVMKESKAKELGLKPMARLVAFA FT SAGVDPAIMGTGPIPASTKCLEKAGWTPADLDLIEANEAFAAQAMSVNQDMGWDLSKVN FT VNGGAIAIGHPIGASGARVLVTLLYEMQKRDAKKGLATLCIGGGQGVALAVERM" FT terminator 4370..4461 FT /gene="phbA" FT CDS 4512..4973 FT /codon_start=1 FT /transl_table=11 FT /note="ORF4" FT /db_xref="InterPro:IPR007897" FT /db_xref="UniProtKB/Swiss-Prot:P45373" FT /protein_id="AAA23323.1" FT /translation="MNSERIIKKYPNRRLYDTEVSRYITLADVRDLVMSGQPFRVLDSA FT NDSDITRSILLQIMLEEETGGQPLFSANMLAQIIRFYGGTLQGTFARYLESSLDLFAKQ FT QQEVTKALTDNPFGTVTRLTQKNVEIWADLQDELMRAAGFPVAPRKKKE" FT RBS 5058..5061 FT /note="for ORF5" FT CDS 5070..5432 FT /codon_start=1 FT /transl_table=11 FT /note="ORF5" FT /db_xref="UniProtKB/Swiss-Prot:P45374" FT /protein_id="AAA23324.1" FT /translation="MNTTDSLKTVNEWTNKSVERMTSFGELNVRLFEKLAARQMDAVNL FT YMDHSMRLMKLATESKGYNDLFKGQVDATKELSERVMAESKATMQFFGDARDEYRVWFE FT KSLNDVSEDLRKSVAV" FT terminator 5455..5515 FT /note="for ORF5" FT RBS 5611..5615 FT /gene="phbB" FT CDS 5621..6361 FT /codon_start=1 FT /transl_table=11 FT /gene="phbB" FT /product="3-hydroxybutyric acid" FT /EC_number="1.1.1.35" FT /EC_number="2.3.1.9" FT /db_xref="GOA:P45375" FT /db_xref="HSSP:1FK8" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P45375" FT /protein_id="AAA23325.1" FT /translation="MARIALVTGGIGGIGTSICTRLAKDGCTVVANCHPSEAAAAEEWK FT QARAAEGFDIAVFTADVSSFDDSARMVREITEQVGPIDILVNCAGITRDKTFKKMEQAH FT WEAVINVNLNSVFNVTRQVWDGMLERGFGRIINISSVNGQRGQFGQANYSAAKAGMHGF FT TMALAQEGASKGVTVNTISPGYVETAMTLAMNDDVRNSIISGIPMRRMAQPNEIAAAIA FT FLAGDESGYMTGANLPVNGGLFMH" FT terminator 6370..6486 FT /gene="phbB" FT CDS 6511..6977 FT /partial FT /codon_start=1 FT /transl_table=11 FT /note="ORF7" FT /db_xref="InterPro:IPR002678" FT /db_xref="UniProtKB/Swiss-Prot:P45371" FT /protein_id="AAA23326.1" FT /translation="MNAVMTDVRDLIRYCDDVLDAARFADYAPNGLQVEGERPLQRLVS FT GVTASAALIEAAIAEHADAILVHHGWFWKNENPCLIGIKGQRARTLLSAGVSLIAYHLP FT LDAHPELGNNATLGRRLDFIDMEPTALANGLLWAAIGSAHDACVLHGACLAS" XX SQ Sequence 6977 BP; 1273 A; 2145 C; 2218 G; 1341 T; 0 other; cgaattcatc cagggccact atctccaact gccgagttcg gacttgaact tcgatttcca 60 gaccctgtca catgaaaccc gatagtctgc tcatgagtac ggcgcttcac acgggacggc 120 tgatcctgac tccatccgat ccgcatctcg cgccggatgt cgatttgttg atcgaaggac 180 tgagccggcc tggattcctg agtgctccat tgatcctgga gaatccacag ccgggggcgg 240 gcgaccgagc ctttgcgact ggcgccggct tcctgagtct actgaccttt gccggctgcg 300 ccgtccggat tcgcgatgac gtcagagcgg ggcgtttctc gcatattcgc atcccgccag 360 tcagtcagca tccaaggttg atggttggcc gtaacacgcg cccaccccga tgtgtcgggt 420 gtcgcgcgcc cctgtcgggc tggcgcgcgc tggtggaaca ctgggccacg catccgcatg 480 ccggcgtgca atgtccggcc tgcggcgaga cacgtccgcc ctggctctgg gactggaaac 540 agcaaggagg attcggacgc gccttcgtgc agatcgaaga ggtctttccg ggtgaggcag 600 tgccgacgcc gatgttcttc gagcagctca ttcgggtcag tgggatcggc tggcgacatt 660 tctacatcca ggattgaatc aagcctggta taggggcgcc ttaacgtgaa ggccagctcc 720 aagtccgctc gatgagcgac gacgcccggt gagtcccgag cgtcatcgtg gatgccgttc 780 gacggctgac gacagcgagc cgccggaatt gctccggcgg ctcgggaagc gttatcgctc 840 gttgagccac ttaccgatgg ccggcgtgac ttctttctgc gccttgccgc tgacatagat 900 gccgatgtga ccgccgggga aggccagctc ggtgtagtcc gggctgctgg tcagtccctt 960 gagcgcccta gaggcatccg gcggcaccag gtgatcctgg agcgcgaaga tgttgagcac 1020 cgggcaggtg atgtccttca ggtccacttc ctgtccgccc agcaccacgc cgccattgag 1080 gaagccgttg ttctggtaga agtccttgat gaactgacgg aaggtctcgc cggcctgatc 1140 cgggctgtcg aagatccatt tctccatgcg caggaagttc ttgaccttgt ccgggtcgtc 1200 gagcagatca accatgttga cgtacttctg acccgtcagg ctgaaaggct tgagcgacag 1260 gaaggtccag ttcagcagct caccggggat gttgcccatg gtgtcgaccg ccagatcgat 1320 gtcgacgttc tgcacccagg ccgagagcag gttgtccggg gtcttgaagt cgaccggcgt 1380 gaccatggtc acgaggttgc gcaccttgtc cgggtgcagc gcgctgtaca tcaggctgaa 1440 ggcgccgccc tgacagatac cgagcagatt gaccttgtcg acgccgtggg cttcgcgcag 1500 atagtccacg cagcggtcga tgtagccgtt gatgtagtcg tcgagcgtga gcgcgcggtc 1560 gccctgatcc ggatagcccc agtcgatcag gtagacgtcc tggccggtgg ccagcagtcc 1620 cttgatggtg gagcggtctt cctggatgtc ggtcatgtag ggccggttga ccagggcgta 1680 gacgatcagg agcggcacgg gctgggcttc gggcgcgccc tcgggtcggt catagcgata 1740 gaggaccagc ttgtcctcgc tgtagacggc ctgcttgggg ctgacgccgg tgtcgatggc 1800 ctcggcgttg agcagattct ccatgccctg acccagcttg cggctgtagt cgagcatctc 1860 ctgggtgagt ttgtccggcc ggatgtcgat ggggaacatg atcgctgtcc tctgattctg 1920 tctggtgagc ctgctgttcg atcagccgct tgacgctcaa tcggccggat tggtcttggt 1980 ggtgcgccgg cgtgccggag ccttggcggc cggggcgggc gacctgagcg cagtggcagg 2040 cttggtggcc ggctcctcac cggccagggc tgcgacgcga cgttccagat cgcgcaggct 2100 gtgacgcagg gccttgttct cgcgacgggt ctcctggagc cggtcctgga gggtgcgcag 2160 ctcgctgcgg gtcggcatgt tcaatgcgcc caggttctcg tcgaccagga tcgacatgcg 2220 cttcttgagc gccatctgag cgttgaccag ccggccgtgg atgcgggcgt attccggtgt 2280 ggcgacctcg tcggcatagg cgccttcgca gcaggcgacc cagttgtcgt agagcgcgcg 2340 cgccgagtcg atggttttgc cgctgtcgat gacaccctgg atgtaggagc ccatgtgctc 2400 gaccgacttc atgccgagct tggtgtagac gttggtgtat tcctggagcg cggcctgata 2460 ctccatggcg ctgcgcatca gctcctgata ctggctctgc tcctcgcggg tgtagccgag 2520 accgggcgcc gagagggctc gatcaagact gtccttgaac tgctcgtggg gcatgttgcg 2580 cagcatgtcg cccggcatcg gcgacatcga ggacatcatg cgctgccagt tgtcgagcgg 2640 cagctcccag aacgacatca ggcgctgcat ggtgttgccg ccgtcgtcga gcgagccgct 2700 gaagcgcttc tgcatgtctt cgagcgtctt ggtccagagc tccaagccgt tgccggcgtt 2760 gccctcgtcg gcgcgtttgg cgaaggtgtc ggcgagacgg aagaagttct tgccctgctc 2820 catcatcttt tccatgaacg agcgtgacag atccggggcg gcgggcgcca tggctttcca 2880 ccactgatcg atggccgcct cccagggcgt gttggccgaa ctggaggcgc tgtccagacc 2940 catggccttg cggctcatgt cggtccagtt gtcccagtac ttgcgttgca gttcgagcca 3000 atcgtcattg aagaaattag tgttgctcat ggacgtaccc tcctcgtggc gcgtggtgtt 3060 gggcgaacgg tatcatgtgg atttgtgcac tgcaacaaag atgcgtagaa tccacaggcc 3120 gcgaccccat atcggggacg ctcgtccatt tccaatcaga atccagagga catccacgcc 3180 atgagcgaga acatcgtcat cgtcgacgcc ggccgcagtg ccatcggaac cttcggcggc 3240 agtctgtcgt cactctcggc caccgagatc ggcaccgccg tgctcaaggg gctgctggcg 3300 cgtaccggac tcgcgccgga acagatcgac gaggtgattc tcggccaggt gctgaccgcc 3360 ggcgtgggcc agaaccccgc ccgtcagacc acgctgcacg cggggctacc gcattcggtg 3420 ccggccatga ccatcaacaa ggtctgcggc agcggtctga aggcggtgca tctggcgatg 3480 caggccatcg cctgcgggga tgccgacatc gtcatcgccg gcggtcagga gagcatgagc 3540 cagtcctcgc acgtcctgcc gcgttcgcgc gacggtcagc gcatgggcga ctggtcgatg 3600 aaggacacca tgatcgtcga cggcctctgg gatgccttca acaactatca catgggcacc 3660 accgccgaga acatcgccca gaagtacggc tttacgcgcg agcagcagga cgccttcgcc 3720 gccgcctcgc agcagaagac cgaggccgcg cagaaggctg gccgcttcca ggacgagatc 3780 attccgatcg agattccgca gcgcaagggc gatccgaagg tgtttgatgc cgacgagttc 3840 ccgcgtcacg gcaccacggc cgagagtctg ggcaagctgc gtccggcctt ctcgaaggac 3900 ggcagcgtca cggcgggtaa cgcctccggc atcaacgacg gggcggccat ggtcgtggtg 3960 atgaaggagt ccaaggccaa ggaactgggt ctgaagccga tggcgcgtct ggtggccttc 4020 gccagcgccg gtgtcgatcc ggcgatcatg gggacgggcc cgatcccggc gtcgaccaag 4080 tgcctggaga aggccggctg gaccccggcg gatctggatc tgatcgaggc caacgaagcc 4140 ttcgccgcgc aggccatgtc ggtcaaccag gacatgggct gggatctgtc caaggtcaac 4200 gtcaacggcg gcgccatcgc catcggtcat ccgatcggcg cctccggtgc gcgcgtgctc 4260 gtgaccctgc tctatgagat gcagaagcgc gacgccaaga agggtctggc gacgctgtgc 4320 atcggcggcg gccagggcgt ggcgctggcg gtcgagcgga tgtgagccgt cgtccgccgg 4380 tctgaatcgc cggcggaccg agcctcctga atcgctccag gcactgaacg ccctgccgat 4440 cccggatcgg tggggcgttt gcgcgcttgg ggtagacttg ccgaacgacc agccgaaccg 4500 cccgggtgcc catgaacagc gagcgcatca tcaagaagta tccgaaccgc cgcctctacg 4560 acaccgaggt cagccgctat atcaccctcg ccgatgtgcg cgatctggtg atgagcggac 4620 agcccttccg cgtcctcgac agcgccaatg acagcgatat cacccgttcc atcctgctcc 4680 agatcatgct ggaggaggag accggcggtc agccgctgtt cagcgccaac atgctggccc 4740 agatcatccg cttctacggc ggcacccttc agggcacctt cgcccgctat ctggaatctt 4800 cactcgacct gttcgccaag cagcaacagg aagtgaccaa ggcactcacc gacaatccct 4860 tcgggacggt gacacgcctg actcagaaga acgtcgagat ctgggctgat ctccaggacg 4920 aactcatgcg cgcggctggc tttccggtcg cgccgcgcaa gaaaaaagaa taatgaggat 4980 tgcgaaaatt gcgcttgacg gccgtcggtc acagctttat tgtgcaatgc aacattgctg 5040 cactgcacaa accttacgga gagatgatca tgaacaccac cgacagcctc aagaccgtca 5100 acgagtggac caacaagagc gtcgagcgca tgaccagctt cggtgagctg aacgtgcgtc 5160 tgttcgagaa gctggccgcc cgtcagatgg acgccgtgaa cctgtacatg gatcacagca 5220 tgcgcctgat gaagctggcc accgagtcca agggttacaa tgacctcttc aagggtcagg 5280 tcgacgccac caaggaactg agcgagcgcg tcatggccga gagcaaggcc accatgcagt 5340 tcttcggcga tgcccgcgac gaataccgcg tgtggttcga gaagagcctg aacgacgtca 5400 gcgaagatct gcgcaagagc gtcgccgtct aaagacgccg acctctgggc catcgcgatc 5460 cagggatgga tcgccattgg tcatgcttcc ggatcggccg ggagcacgcc caatggaacc 5520 aacgcttcac cttgcctgcc gcttggtagt aaagtggcct tgaagttcga cgacactgtt 5580 catcgttctc aatagttcca aagattcctg gaggaacccc atggctcgta tcgcactcgt 5640 caccggcggc atcggcggca tcggcacttc gatctgcaca cgcctggcaa aggatggctg 5700 caccgtcgtg gcgaactgcc atccgtccga ggcggccgcc gccgaagagt ggaagcaggc 5760 ccgtgccgcc gaggggttcg acatcgccgt cttcaccgct gacgtgtcct cgttcgacga 5820 cagcgcgcgc atggttcgcg agatcacaga gcaggtcggt cccatcgaca tcctggtcaa 5880 ctgtgccggc atcacccgcg acaagacctt caagaagatg gagcaggcgc actgggaggc 5940 cgtgatcaac gtcaacctca acagcgtctt caacgtcacc cgtcaggtgt gggacgggat 6000 gctggagcgc ggcttcgggc gtatcatcaa catctcgtcg gtcaacggtc agcgcggcca 6060 gttcggtcag gccaactatt ccgccgccaa ggccggtatg cacggcttca ccatggctct 6120 ggctcaggag ggtgcgtcca agggcgtgac cgtcaacacc atctcgcccg gctatgtcga 6180 gacggccatg accctggcga tgaacgacga tgtgcgcaac agcatcatca gcggtattcc 6240 gatgcgtcgc atggctcagc ctaatgagat cgccgccgcc atcgctttcc tggccggcga 6300 cgagagcggt tatatgacgg gcgccaatct gccggtcaac ggcggtctgt tcatgcattg 6360 atttagatca taccgggccg aatacaaaac actgacaatg gcccggtgtt tcaggatctc 6420 accgagtcct cctcgtctct ctcatcatga gacgtttaca gcccggcgcc agccgggctt 6480 ttttttgtgt agaatcgaat cgcccatccg atgaacgctg tgatgaccga cgtacgcgat 6540 ctgatccgct actgcgatga cgtgctcgac gcggcgcgct tcgccgacta tgcgccgaat 6600 ggtttgcaag tggagggcga acggccgctc cagcggctgg tgtcgggcgt gacggccagc 6660 gccgcgttga tcgaggccgc gatcgcggag cacgccgacg ccattctggt ccatcatggc 6720 tggttctgga agaacgagaa tccctgcctg atcggcatca aggggcagcg cgcaaggaca 6780 ttgctcagcg cgggtgtgag tctgatcgcc tatcatctgc cgctcgatgc ccatcccgaa 6840 ctcggcaaca atgccacact cggtcgccgg ctcgatttca tcgacatgga accgaccgca 6900 ctggccaatg gtctgctgtg ggcggcgatt ggctcagccc atgacgcctg cgtccttcac 6960 ggagcatgtc tcgcatc 6977 // |
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