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EBI DbfetchID K02124; SV 1; linear; genomic DNA; STD; PHG; 2129 BP. XX AC K02124; X01670; XX DT 31-JUL-1991 (Rel. 28, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 5) XX DE Bacteriophage SPR DNA methyltransferase gene, complete cds. XX KW . XX OS Bacillus phage SPR OC Viruses; dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae; OC Lambda-like viruses; unclassified Lambda-like viruses. XX RN [1] RP 1-2129 RX DOI; 10.1016/0378-1119(84)90165-3 RX PUBMED; 6092231. RA Buhk H.J., Behrens B., Tailor R., Wilke K., Prada J.J., Gunthert U., RA Noyer-Weidner M., Jentsch S., Trautner T.A.; RT "Restriction and modification in Bacillus subtilis: nucleotide sequence, RT functional organization and product of the DNA methyltransferase gene of RT bacteriophage SPR"; RL Gene 29(1-2):51-61(1984). XX RN [2] RP 289-2124 RX DOI; 10.1093/nar/12.23.9039 RX PUBMED; 6096817. RA Posfai G., Baldauf F., Erdei S., Posfai J., Venetianer P., Kiss A.; RT "Structure of the gene coding for the sequence-specific RT DNA-methyltransferase of the B. subtilis phage SPR"; RL Nucleic Acids Res. 12(23):9039-9049(1984). XX RN [3] RP 1-2129 RA Trautner T.A.; RT ; RL Unpublished. XX CC On Aug 4, 2004 this sequence version replaced gi:14873. CC Draft entry and sequence for [1],[Unpublished (1985) CC Max-Planck-Institut fuer Molekulare Genetik,Ihnestrasse 63-73] CC kindly provided by A.Trautner, CC 15-MAR-1985. Sequence shown is from the hybrid phage SPP1. The CC molecular weight of the methyltransferase predicted from the CC nucleotide sequence is in agreement with that established CC experimentally. CC Mutant SPR19 affects the methylation of 'ggcc'. Mutant SPR83 CC affects the methylation of 'ccgg'. Mutant SPR26 affects the CC methylation of both (Trautner et al., Mol. Gen. Genet. 180, 361-367 CC (1980)). XX FH Key Location/Qualifiers FH FT source 1..2129 FT /organism="Bacillus phage SPR" FT /host="Bacillus subtilis" FT /strain="TN106" FT /mol_type="genomic DNA" FT /clone="pBH100; pBH101; pSU21" FT /db_xref="taxon:10725" FT -35_signal 541..546 FT /inference="non-experimental evidence, no additional FT details recorded" FT -35_signal 554..563 FT /inference="non-experimental evidence, no additional FT details recorded" FT -10_signal 565..570 FT /inference="non-experimental evidence, no additional FT details recorded" FT mRNA 578..>1924 FT /product="DNA methyltransferase" FT -10_signal 582..588 FT /inference="non-experimental evidence, no additional FT details recorded" FT mRNA 592..>1924 FT /product="DNA methyltransferase" FT CDS 605..1924 FT /codon_start=1 FT /transl_table=11 FT /product="DNA methyltransferase" FT /note="Mtase" FT /db_xref="GOA:P00476" FT /db_xref="HSSP:6MHT" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P00476" FT /protein_id="AAA32604.1" FT /translation="MGKLRVMSLFSGIGAFEAALRNIGVGYELVGFSEIDKYAVKSFCA FT IHNVDEQLNFGDVSKIDKKKLPEFDLLVGGSPCQSFSVAGHRKGFEDTRGTLFFQYVET FT LKEKQPKFFVFENVKGLINHDKGNTLNVMAEAFSEVGYRIDLELLNSKFFNVPQNRERL FT YIIGIREDLIKNEEWSLDFKRKDILQKGKQRLVELDIKSFNFRWTAQSAATKRLKDLLE FT EYVDEKYYLNEDKTNSLIKELSTSRLNENLTVEQVGNINPSGNGMNGNVYNSSGLSPTI FT TTNKGEGLKIAVEYSRKSGLGRELAVSHTLSASDWRGLNRNQKQNAVVEVRPVLTPERG FT EKRQNGRRFKDDGEPAFTVNTIDRHGVAVGEYPKYRIRRLTPLECFRLQAFDDEDFEKA FT FAAGISNSQLYKQAGNSITVTVLESIFKELIHTYVNKESE" FT variation 878 FT /replace="a" FT /note="a in muntant SPR26" FT variation 1503 FT /replace="a" FT /note="a in mutant SPR83" FT variation 1613 FT /replace="t" FT /note="t in mutant SPR19" XX SQ Sequence 2129 BP; 782 A; 263 C; 472 G; 612 T; 0 other; cttaacaatg tggaaatcaa ctgtaccaat cggatcgcct agttgtttaa cttggtcaat 60 tgatttaaca ggcgtaagag ctgaggcttg tgatgtgaaa gcagggagag ccagagtggt 120 tagagataag gcagaaacaa tcaatccttt gtaaaacttt ttcataagaa ttacctccca 180 ggttttgatt gtgatacaac tctagtctag catggaaaat atttgaaatg tgtgaagtgt 240 ttgtgaaact gattaaaata tctcttttat taggaagggt ggataagggt tgcttaaatt 300 aaaatttaga aatcaaacac tggaattgcg tagaaaaatg tccttacaat tacaccgttt 360 attagaagac atgacagctt ggtcatttaa tgaaaatgaa gctcaaaaat atattgatga 420 atatgaatta gagttagttg agaagagtgt agacaataaa tttaagcttc aaatagtaga 480 taagaataaa aatatttggg gcgaattaaa acccaaagag ccagggtaca ttggatattt 540 tgtacagact gaataaatga tcaattttat ttagagtaaa aataaaatat atggaggttg 600 tttattgggt aaactacgtg taatgagtct ttttagtggg atcggtgcat ttgaagctgc 660 actaagaaac attggggttg gttatgagct ggttggtttt agtgagattg ataaatatgc 720 cgtcaaatct ttttgtgcaa ttcacaacgt tgatgagcaa ttaaattttg gagatgtaag 780 caagattgat aagaaaaaac tacctgaatt tgatctttta gttggaggat ctccttgtca 840 aagctttagt gtagccggcc atcgaaaggg atttgaagat acaagaggga cattgttttt 900 tcaatacgtt gagactctta aggaaaagca accaaagttt tttgtttttg aaaatgttaa 960 agggttgatc aaccatgata aaggaaatac attaaatgtt atggctgaag ctttcagtga 1020 agttgggtac agaattgacc tagagctgct taattcaaaa ttctttaatg ttccacaaaa 1080 tagggagcga ctttacataa ttggaattag agaagattta attaaaaatg aagaatggtc 1140 tttggatttt aaaagaaagg atatacttca aaaagggaaa cagagattgg tagaattaga 1200 tattaaaagc tttaatttta gatggacagc tcaatcggct gctacgaaga ggctaaaaga 1260 tttattagaa gaatacgttg atgaaaagta ctacttgaat gaagataaaa caaacagttt 1320 gatcaaagag ttgtctacaa gtcgacttaa tgaaaatctt actgttgagc aagtaggtaa 1380 cattaatccc tctggtaatg gaatgaatgg aaatgtttat aattcatctg gattaagccc 1440 cacaattacc actaataaag gagagggact gaaaattgca gttgagtact ccagaaaaag 1500 cgggcttgga cgagaacttg ctgtatctca tacgctttct gcttctgact ggagaggatt 1560 gaataggaac caaaaacaaa atgcagttgt tgaggtaagg ccagtattaa ccccagaaag 1620 gggggagaag cgacaaaatg gaagaagatt taaagatgac ggtgaaccag catttacagt 1680 aaacacaatt gacagacacg gggtagcggt tggagagtat ccaaaataca gaattagaag 1740 attaacaccg ttagagtgct ttaggctaca ggcttttgat gacgaagatt ttgaaaaagc 1800 ttttgctgcg ggaataagta actcacaatt atataagcaa gccggtaatt caattactgt 1860 aactgtgctt gagtcaatat tcaaggaatt aatacataca tacgttaata aagaatctga 1920 ataaaatttg tcttttaaac aaatgtaaaa taagaggagt ggattgattg gaaagttacc 1980 ctgagtcctt aaaaagagag acagaggaga ttaaagagcg tgttaggaat ggaaatatca 2040 aagaagacag gattaaagaa attgcagaaa cgacagttga gtttttgaaa tcagaggaga 2100 aaagacataa atacttttct gaagttgct 2129 // |
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