spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   K02118; SV 1; linear; genomic DNA; STD; UNC; 293 BP.
XX
AC   K02118; M27532;
XX
DT   13-JUN-1985 (Rel. 06, Created)
DT   15-JAN-2007 (Rel. 90, Last updated, Version 4)
XX
DE   Plasmid R67 from E.coli type II dihydrofolate-reductase (DHFR) gene,
DE   complete cds.
XX
KW   dihydrofolate reductase; methotrexate-resistant; plasmid.
XX
OS   Plasmid R67
OC   other sequences; plasmids.
OG   Plasmid R67
XX
RN   [1]
RP   1-293
RX   DOI; 10.1016/0378-1119(84)90266-X
RX   PUBMED; 6735180.
RA   Brisson N., Hohn T.;
RT   "Nucleotide sequence of the dihydrofolate-reductase gene borne by the
RT   plasmid R67 and conferring methotrexate resistance";
RL   Gene 28(2):271-274(1984).
XX
CC   The type II dihydrofolate reductase (DHFR) enzymes are insensitive
CC   to methotrexate and trimethoprim and appear to have an origin
CC   distinct from other DHFRs.
CC   [1] compared sequences with another type II-DHFR enzyme, R388 (see
CC   separate entry), and found sequence homology of 75%, with most of
CC   the changes clustered in the 5' and 3' regions. Also reported in
CC   [1] was a modified gene sequence, designated R67' (see separate
CC   entry). After modification of the 3' end of the R67 gene,
CC   trimethoprim resistance in E.coli was observed to the same degree
CC   as in the unmodified gene. These findings support the idea that a
CC   conserved region of the gene may encode the active site of the
CC   protein.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..293
FT                   /organism="Plasmid R67"
FT                   /plasmid="R67"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:2494"
FT   CDS             40..276
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="dihydrofolate-reductase"
FT                   /db_xref="GOA:P00383"
FT                   /db_xref="InterPro:IPR008990"
FT                   /db_xref="InterPro:IPR009159"
FT                   /db_xref="PDB:1VIE"
FT                   /db_xref="PDB:1VIF"
FT                   /db_xref="PDB:2GQV"
FT                   /db_xref="PDB:2P4T"
FT                   /db_xref="PDB:2RH2"
FT                   /db_xref="PDB:2RK1"
FT                   /db_xref="PDB:2RK2"
FT                   /db_xref="UniProtKB/Swiss-Prot:P00383"
FT                   /protein_id="AAA26083.1"
FT                   /translation="MERSSNEVSNPVAGNFVFPSNATFGMGDRVRKKSGAAWQGQIVGW
FT                   YCTNLTPEGYAVESEAHPGSVQIYPVAALERIN"
XX
SQ   Sequence 293 BP; 78 A; 71 C; 80 G; 64 T; 0 other;
     ccacatagaa cacctagaag ttcacaagaa aggtcggaaa tggaacgaag tagcaatgaa        60
     gtcagtaatc cagttgctgg caattttgta ttcccatcga acgccacgtt tggtatggga       120
     gatcgcgtgc gcaagaaatc cggcgccgcc tggcaaggtc agattgtcgg gtggtactgc       180
     acaaatttga cccccgaagg ctacgccgtc gagtctgagg ctcacccagg ctcagtacag       240
     atttatcctg ttgcggcgct tgaacgcatc aactgagttc aaggttgaag tgc              293
//
spacer
spacer