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EBI DbfetchID K02081; SV 1; linear; genomic DNA; STD; PRO; 3889 BP. XX AC K02081; XX DT 13-JUN-1985 (Rel. 06, Created) DT 02-SEP-1999 (Rel. 60, Last updated, Version 3) XX DE P.stuartii PstI restriction and modification genes, complete. XX KW endonuclease; methylase; modification enzyme; restriction endonuclease. XX OS Providencia stuartii OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Providencia. XX RN [1] RP 1-3889 RX PUBMED; 6330092. RA Walder R.Y., Walder J.A., Donelson J.E.; RT "The organization and complete nucleotide sequence of the PstI RT restriction-modification system"; RL J. Biol. Chem. 259(12):8015-8026(1984). XX CC The two genes for the PstI restriction-modification system are CC encoded on opposite strands and are transcribed from separate CC promoters. The restriction endonuclease contains 326 amino acids. CC The modification enzyme contains 507 amino acids. The close CC proximity of the transcription initiation points of the two genes CC (70 bp) suggests that the promoters overlap. The -10 regions for CC the two genes are found at bp 2307-2302 (MME) and 2352-2357 (PstI). CC Bases 2267-2340 are protected from DNaseI digestion. The PstI CC restriction-modification system is a type II system. The two CC enzymes both recognize the hexanucleotide sequence 'ctgcag'. The CC modification enzyme protects this sequence from cleavage by PstI by CC methylation of its adenosine residue. PstI cleaves the DNA between CC the adenosine and guanine residues, leaving 'sticky ends' of 4 bp. XX FH Key Location/Qualifiers FH FT source 1..3889 FT /organism="Providencia stuartii" FT /mol_type="genomic DNA" FT /db_xref="taxon:588" FT mRNA complement(<742..2292) FT /note="MME mRNA" FT CDS complement(742..2265) FT /codon_start=1 FT /transl_table=11 FT /note="methylase modification enzyme (MME)" FT /db_xref="GOA:P00474" FT /db_xref="InterPro:IPR002052" FT /db_xref="UniProtKB/Swiss-Prot:P00474" FT /protein_id="AAA25672.1" FT /translation="MTEAATQLPISLNILVDSIREAANSTLDETLRSKLGQFMSSSAVS FT ELMANLFESYVGEHEILDAGAGVGSLTAAFVQNATLNGAKSISSTCYEISEVMVYNLIQ FT VLDLCKIRAMEFEVNWQQKIIESDFIQASVEQLLIENYSPKYNKAILNPPYLKIAAKGR FT ERALLQKVGIEASNLYSAFVALAIKQLKSGGELVAITPRSFCNGPYFNDFRKQMLDECS FT LNKIHVFNSRKSAFKADNVLQENIIYHLTKGETQRKVVTVYSSTCANDINPTIFEVPFD FT EIVKSNNPDLFIHIVTNEQERELANKAGGLPCSLSDLGIQVSTGKVVDFRTRENLSMEY FT ISNSVPLIFPQHLQRCSIVWPITKAKKPNALIVNEATNNLMVPNGIYVLTRRLTAKEEK FT RRIVASIYYPDIANVDTVGFDNKINYFHANGKPLDISLAKGLWVFLNSTLIDKYFRQMN FT GHTQVNATDLRALRYPTREQLEDIANQVDFGEFEQTKIDEIINQSLQLM" FT mRNA 2363..>3376 FT /note="PstI mRNA" FT CDS 2396..3376 FT /codon_start=1 FT /transl_table=11 FT /note="PstI restriction endonuclease (PstI)" FT /db_xref="GOA:P00640" FT /db_xref="InterPro:IPR009528" FT /db_xref="UniProtKB/Swiss-Prot:P00640" FT /protein_id="AAA25673.1" FT /translation="MKELKLKEAKEILKALGLPPQQYNDRSGWVLLALANIKPEDSWKE FT AKAPLLPTVSIMEFIRTEYGKDYKPNSRETIRRQTLHQFEQARIVDRNRDLPSRATNSK FT DNNYSLNQVIIDILHNYPNGNWKELIQQFLTHVPSLQELYERALARDRIPIKLLDGTQI FT SLSPGEHNQLHADIVHEFCPRFVGDMGKILYIGDTASSRNEGGKLMVLDSEYLKKLGVP FT PMSHDKLPDVVVYDEKRKWLFLIEAVTSHGPISPKRWLELEAALSSCTVGKVYVTAFPT FT RTEFRKNAANIAWETEVWIADNPDHMVHFNGDRFLGPHDKKPELS" XX SQ Sequence 3889 BP; 1231 A; 701 C; 676 G; 1281 T; 0 other; aagcttgaaa gacatcaaat ttacccttaa gaaagatttc aataaccctt gaatcatttg 60 catgattaat caagacgata acatgtgata actatttgtt tggttaaata ataattaata 120 cattatttat cacataaaac atatatatag aggttattac tattcgtgac aaatctttct 180 tagcgactca gaggatttac agtaaattga aagggaatca gtccatgaac tgcataaatt 240 tgcaataatt cagcataaaa aaggcactcg cccccgagtg cctttgggta caacctactg 300 aattggtatg atttacacct cttcagcggt tctggtactt gtacggatga ggtaatcaaa 360 ggcgctaagt gcggcgttct aaaagtgagt taaacaattg atttaaatgg atttagtcaa 420 tggttgatgt ccacatattg accatactta taaagcctct tttgacgaga ggctttattt 480 ttgcttattt ttcaatgggt atgaggagtt ttttagcttg ctcaatcaaa gtttcaatta 540 cctcaagagg aatgttcggc tcaaaacaag ttaatgtaaa ttggtgggta acatccgagt 600 aaaagacttc agcaagttgt aacactgggt cagtatttgc catagttagt tcaagataca 660 taccgtctct aactatgtcg ctgcctcttc caaattcgta tttgatgcca ttatgagtca 720 tgtttacctc gttaaatgac gttacattag ctgcaaactc tgattgatta tttcatcaat 780 ttttgtttgt tcaaactcac caaaatcaac ttggttagca atatcttcta attgctctct 840 ggttgggtaa cgtaatgctc gtaaatctgt agcatttact tgagtatgcc cgttcatttg 900 cctgaaatat ttatctatta aagtactgtt tagaaatacc cacaaacctt tagctaagct 960 aatatcaagc ggttttccat tagcatgaaa gtaattaatt ttattatcaa aaccaactgt 1020 atcaacatta gctatatcag gataataaat agaagcaact atccttcttt tctcttcttt 1080 cgctgttaat cgacgtgtaa gaacatatat gccatttggc accattagat tattagtagc 1140 ttcattaacg attaatgcat ttggtttttt agctttggtt attggccata caatagaaca 1200 tctttgtaaa tgttgaggaa atatcaatgg aacagaatta gatatatatt ccatagaaag 1260 gttttcacga gttctaaaat caactacttt gccagtactc acttgaatac ctaaatccga 1320 taatgagcaa ggcaatcctc ctgctttatt tgctaattcc ctttcctgtt cattagtaac 1380 tatatgaata aataagtcgg gattatttga ttttacaatt tcatcaaaag gaacttcaaa 1440 aattgtcgga tttatatcgt tagcacatgt acttgaatat acagtaacaa ccttacgttg 1500 agtttcacct ttagtaaggt gataaattat attttcttgc aatacattat cggctttaaa 1560 agcagacttt cgactgttaa aaacatgaat tttattcaat gaacactcat ctaacatctg 1620 ttttcgaaag tcattaaaat atggtccatt acagaatgaa cgaggagtaa tagccactaa 1680 ctcaccgcct gatttaagct gtttaatagc aagagcaaca aaagcagaat aaagattact 1740 tgcttctata ccaacttttt gtagtaaggc tcgttcacga cctttagccg ctattttaag 1800 gtatggaggg tttaaaatag ccttattata tttcggagag taattttcta taagtaattg 1860 ttcaacagat gcctgaatga aatcactttc tataattttc tgttgccaat taacttcaaa 1920 ctccatagct ctgattttac aaagatcaag aacttgaatt aaattgtaga ccataacttc 1980 tgagatttca tagcatgtcg aacttatgct tttcgcaccg tttagagtcg cattttgtac 2040 aaatgctgct gtcaatgaac caacaccagc tccagcgtct aaaatctcat gctccccaac 2100 ataagattca aataaatttg ccattaattc agacaccgct gatgacgaca tgaactgccc 2160 taattttgag cgaagggttt catctagagt gctattcgca gcctctctta ttgagtctac 2220 taatatatta agagatatag gtaattgtgt tgccgcttca gtcatgcaat gtacatttat 2280 acagtgatat ttaatatgat gagtatctca aaaaagcctc acttaataaa gcaaaatgcc 2340 ttcatatagt gtagagttga ccaactttgt atcacaattt actgaattta aagaaatgaa 2400 agaactaaaa ttaaaagaag ctaaagagat actaaaagcg cttggtttgc caccccaaca 2460 gtataatgat agatctggtt gggttctatt agctttggct aatattaagc ctgaagatag 2520 ctggaaagaa gccaaagccc ctttattacc gacagtaagc atcatggagt ttatccgaac 2580 tgaatacggt aaggattata aaccaaattc gcgtgaaaca atcaggcgtc aaactttaca 2640 tcagtttgaa caagctcgaa ttgttgatag aaatagagat cttccctctc gagccacaaa 2700 cagcaaagat aataattatt ctttaaatca agtaattatt gatattcttc ataattatcc 2760 caatggcaat tggaaagagt taattcaaca gtttttaacc catgttccga gtcttcaaga 2820 gttatatgaa cgagcactcg ctagagatag aatcccaata aaattattag atggtacaca 2880 aatatctctt tcaccgggag agcataacca acttcatgct gatatcgttc atgagttttg 2940 ccctcgtttc gttggtgata tgggtaaaat cttatatatt ggtgatactg caagtagtcg 3000 taatgagggt ggaaaattaa tggttttaga tagcgaatat ttaaaaaaat taggtgtgcc 3060 ccctatgtca cacgacaaat tgccagatgt tgttgtgtac gatgaaaagc gtaaatggtt 3120 atttttaata gaagcggtta catcccatgg cccaattagt cctaaacgtt ggctagaact 3180 tgaagctgcc ttgtcatcat gtacagtcgg taaagtgtat gttactgctt tcccaacacg 3240 tacagagttc agaaaaaatg cggcaaacat tgcatgggaa accgaggttt ggattgctga 3300 taatccagat catatggtcc atttcaatgg cgaccgtttc ttaggcccac atgataaaaa 3360 accagaatta agttaattaa gatggaatag ttcaaaacat ctccatctgt ttctctcccc 3420 gatgtgatct aggtagtttt tccatcggga tctcaaaacc tatcggtaat tcttgcgggg 3480 ttggtatagt taaaaagatt tccactgagg ttattgtcgt aaaagaacag ccacacaaaa 3540 tattctgaca ttgatggtaa cttctacggg gctcactact catggattta ctagttttgg 3600 tataagtcac agcaccacgc tttggataat tgaacgccat tttataacct tgggttatgg 3660 gggtgcctga cgttattata atcaattatg tttcgttttg ctcgtcatcc gtcatatcaa 3720 tatttttgat ttttatttcc agctccaaat gggttacaaa cccgttccat caatggtgtg 3780 tatcactcgg ctaatcaccc ttttttgctc gatttctctt ttttaggctt ttgcttgcgt 3840 ttaagattgg ttttcttttg ttgcccatgg ttcaaatcaa gccaataag 3889 // |
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