EBI Dbfetch

ID   J04987; SV 1; linear; genomic DNA; STD; PRO; 2328 BP.
XX
AC   J04987;
XX
DT   19-APR-1990 (Rel. 23, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 7)
XX
DE   A.eutrophus beta-ketothiolase (phbA) and acetoacetyl-CoA reductase (phbB)
DE   genes, complete cds.
XX
KW   acetoacetyl-CoA reductase; acetyl-CoA acetyltransferase; beta-ketothiolase.
XX
OS   Cupriavidus necator
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
XX
RN   [1]
RP   1-2328
RX   PUBMED; 2670935.
RA   Peoples O.P., Sinskey A.J.;
RT   "Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16.
RT   Characterization of the genes encoding beta-ketothiolase and
RT   acetoacetyl-CoA reductase";
RL   J. Biol. Chem. 264(26):15293-15297(1989).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2328
FT                   /organism="Cupriavidus necator"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:106590"
FT   RBS             29..32
FT                   /note="ribosome binding site"
FT   CDS             40..1221
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="phbA"
FT                   /product="beta-ketothiolase"
FT                   /EC_number="2.3.1.16"
FT                   /db_xref="GOA:P14611"
FT                   /db_xref="HSSP:1M3K"
FT                   /db_xref="InterPro:IPR016038"
FT                   /db_xref="UniProtKB/Swiss-Prot:P14611"
FT                   /protein_id="AAA21972.1"
FT                   /translation="MTDVVIVSAARTAVGKFGGSLAKIPAPELGAVVIKAALERAGVKP
FT                   EQVSEVIMGQVLTAGSGQNPARQAAIKAGLPAMVPAMTINKVCGSGLKAVMLAANAIMA
FT                   GDAEIVVAGGQENMSAAPHVLPGSRDGFRMGDAKLVDTMIVDGLWDVYNQYHMGITAEN
FT                   VAKEYGITREAQDEFAVGSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEFVRQ
FT                   GATLDSMSGLKPAFDKAGTVTAANASGLNDGAAAVVVMSAAKAKELGLTPLATIKSYAN
FT                   AGVDPKVMGMGPVPASKRALSRAEWTPQDLDLMEINEAFAAQALAVHQQMGWDTSKVNV
FT                   NGGAIAIGHPIGASGCRILVTLLHEMKRRDAKKGLASLCIGGGMGVALAVERK"
FT   RBS             1286..1289
FT                   /note="ribosome binding site"
FT   CDS             1296..2036
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="phbB"
FT                   /product="acetoacetyl-CoA reductase"
FT                   /EC_number="1.1.1.36"
FT                   /db_xref="GOA:P14697"
FT                   /db_xref="HSSP:1GZ6"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:P14697"
FT                   /protein_id="AAA21973.1"
FT                   /translation="MTQRIAYVTGGMGGIGTAICQRLAKDGFRVVAGCGPNSPRREKWL
FT                   EQQKALGFDFIASEGNVADWDSTKTAFDKVKSEVGEVDVLINNAGITRDVVFRKMTRAD
FT                   WDAVIDTNLTSLFNVTKQVIDGMADRGWGRIVNISSVNGQKGQFGQTNYSTAKAGLHGF
FT                   TMALAQEVATKGVTVNTVSPGYIATDMVKAIRQDVLDKIVATIPVKRLGLPEEIASICA
FT                   WLSSEESGFSTGADFSLNGGLHMG"
FT   misc_signal     2038..2065
FT                   /note="pot. transcription termination signal; putative"
XX
SQ   Sequence 2328 BP; 414 A; 744 C; 797 G; 373 T; 0 other;
     ctgcaggttc cctcccgttt ccattgaaag gactacacaa tgactgacgt tgtcatcgta        60
     tccgccgccc gcaccgcggt cggcaagttt ggcggctcgc tggccaagat cccggcaccg       120
     gaactgggtg ccgtggtcat caaggccgcg ctggagcgcg ccggcgtcaa gccggagcag       180
     gtgagcgaag tcatcatggg ccaggtgctg accgccggtt cgggccagaa ccccgcacgc       240
     caggccgcga tcaaggccgg cctgccggcg atggtgccgg ccatgaccat caacaaggtg       300
     tgcggctcgg gcctgaaggc cgtgatgctg gccgccaacg cgatcatggc gggcgacgcc       360
     gagatcgtgg tggccggcgg ccaggaaaac atgagcgccg ccccgcacgt gctgccgggc       420
     tcgcgcgatg gtttccgcat gggcgatgcc aagctggtcg acaccatgat cgtcgacggc       480
     ctgtgggacg tgtacaacca gtaccacatg ggcatcaccg ccgagaacgt ggccaaggaa       540
     tacggcatca cacgcgaggc gcaggatgag ttcgccgtcg gctcgcagaa caaggccgaa       600
     gccgcgcaga aggccggcaa gtttgacgaa gagatcgtcc cggtgctgat cccgcagcgc       660
     aagggcgacc cggtggcctt caagaccgac gagttcgtgc gccagggcgc cacgctggac       720
     agcatgtccg gcctcaagcc cgccttcgac aaggccggca cggtgaccgc ggccaacgcc       780
     tcgggcctga acgacggcgc cgccgcggtg gtggtgatgt cggcggccaa ggccaaggaa       840
     ctgggcctga ccccgctggc cacgatcaag agctatgcca acgccggtgt cgatcccaag       900
     gtgatgggca tgggcccggt gccggcctcc aagcgcgccc tgtcgcgcgc cgagtggacc       960
     ccgcaagacc tggacctgat ggagatcaac gaggcctttg ccgcgcaggc gctggcggtg      1020
     caccagcaga tgggctggga cacctccaag gtcaatgtga acggcggcgc catcgccatc      1080
     ggccacccga tcggcgcgtc gggctgccgt atcctggtga cgctgctgca cgagatgaag      1140
     cgccgtgacg cgaagaaggg cctggcctcg ctgtgcatcg gcggcggcat gggcgtggcg      1200
     ctggcagtcg agcgcaaata aggaaggggt tttccggggc cgcgcgcggt tggcgcggac      1260
     ccggcgacga taacgaagcc aatcaaggag tggacatgac tcagcgcatt gcgtatgtga      1320
     ccggcggcat gggtggtatc ggaaccgcca tttgccagcg gctggccaag gatggctttc      1380
     gtgtggtggc cggttgcggc cccaactcgc cgcgccgcga aaagtggctg gagcagcaga      1440
     aggccctggg cttcgatttc attgcctcgg aaggcaatgt ggctgactgg gactcgacca      1500
     agaccgcatt cgacaaggtc aagtccgagg tcggcgaggt tgatgtgctg atcaacaacg      1560
     ccggtatcac ccgcgacgtg gtgttccgca agatgacccg cgccgactgg gatgcggtga      1620
     tcgacaccaa cctgacctcg ctgttcaacg tcaccaagca ggtgatcgac ggcatggccg      1680
     accgtggctg gggccgcatc gtcaacatct cgtcggtgaa cgggcagaag ggccagttcg      1740
     gccagaccaa ctactccacc gccaaggccg gcctgcatgg cttcaccatg gcactggcgc      1800
     aggaagtggc gaccaagggc gtgaccgtca acacggtctc tccgggctat atcgccaccg      1860
     acatggtcaa ggcgatccgc caggacgtgc tcgacaagat cgtcgcgacg atcccggtca      1920
     agcgcctggg cctgccggaa gagatcgcct cgatctgcgc ctggttgtcg tcggaggagt      1980
     ccggtttctc gaccggcgcc gacttctcgc tcaacggcgg cctgcatatg ggctgacctg      2040
     ccggcctggt tcaaccagtc ggcagccggc gctggcgccc gcgtattgcg gtgcagccag      2100
     cgcggcgcac aaggcggcgg gcgtttcgtt tcgccgcccg tttcgcgggc cgtcaaggcc      2160
     cgcgaatcgt ttctgcccgc gcggcattcc tcgctttttg cgccaattca ccgggttttc      2220
     cttaagcccc gtcgcttttc ttagtgcctt gttgggcata gaatcagggc agcggcgcag      2280
     ccagcaccat gttcgtgcag cgcggccctc gcgggggcga ggctgcag                   2328
//