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EBI DbfetchID J04114; SV 1; linear; genomic DNA; STD; PRO; 3523 BP. XX AC J04114; XX DT 22-APR-1989 (Rel. 19, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE B.japonicum uptake hydrogenase 34.5 and 65.9 kd subunit (hupS and hupL) DE genes, complete cds. XX KW uptake hydrogenase. XX OS Bradyrhizobium japonicum OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Bradyrhizobiaceae; Bradyrhizobium. XX RN [1] RP 1-3523 RX DOI; 10.1073/pnas.85.22.8395 RX PUBMED; 3054886. RA Sayavedra-Soto L.A., Powell G.K., Evans H.J., Morris R.O.; RT "Nucleotide sequence of the genetic loci encoding subunits of RT Bradyrhizobium japonicum uptake hydrogenase"; RL Proc. Natl. Acad. Sci. U.S.A. 85(22):8395-8399(1988). XX DR GOA; P21960. DR InterPro; IPR000516; Ni-dep_Hydgase_cyt-B. DR UniProtKB/Swiss-Prot; P21960; CYBH_BRAJA. XX CC Draft entry and printed copy of sequence for [1] kindly provided by CC H.J.Evans, 15-NOV-1988. XX FH Key Location/Qualifiers FH FT source 1..3523 FT /organism="Bradyrhizobium japonicum" FT /mol_type="genomic DNA" FT /db_xref="taxon:375" FT mRNA 256..>1360 FT /note="hup mRNA" FT CDS 269..1360 FT /codon_start=1 FT /transl_table=11 FT /note="uptake hydrogenase (hupS) precursor" FT /db_xref="GOA:P12635" FT /db_xref="HSSP:1FRF" FT /db_xref="InterPro:IPR017909" FT /db_xref="UniProtKB/Swiss-Prot:P12635" FT /protein_id="AAA26218.1" FT /translation="MGAATETFYSVIRRQGITRRSFHKFCSLTATSLGLGPLAASRIAN FT ALETKPRVPVIWMHGLECTCCSESFIRSAHPLVKDAVLSMISLDYDDTIMAAAGHQAEA FT ILEETRAKHKGQYILAVEGNPPLNEGGMFCIDGGKPFVEKLKMMAEDAMAIIAWGACAS FT WGCVQAAKPNPTQATPIDKVITNKPIIKVPGCPPIAEVMTGVVTFITTFGKLPELDRQG FT RPKMFYSQRIHDKCYRRPHFDAGQFVEEWDDEAARKGYCLYKMGCKGPTTYNACSTVRW FT NGGVSFPIQSGHGCIGCSEDGFWDKGSFYDRLTNIKQFGIEKNADQIGMVAAGAVGAAV FT AAHAAVTAVKRLATKREDADHNS" FT sig_peptide 269..406 FT /note="uptake hydrogenase (hupS) signal peptide" FT mat_peptide 407..1357 FT /note="uptake hydrogenase (hupS)" FT CDS 1390..3180 FT /codon_start=1 FT /transl_table=11 FT /note="uptake hydrogenase (hupL)" FT /db_xref="GOA:P12636" FT /db_xref="HSSP:1H2R" FT /db_xref="InterPro:IPR018194" FT /db_xref="UniProtKB/Swiss-Prot:P12636" FT /protein_id="AAA26219.1" FT /translation="MGIQTPNGFNLDNSGKRIVVDPVTRIEGHMRVEVNVDADNVIRNA FT VSTGTMWRGIEVILKNRDPRDAWAFTERICGVCTGTHALTSVRAVENALGITIPENANS FT IRNLMQLALQVHDHVVHFYHLHALDWVDVVSALSADPRATSTLAQSISNWPLSSPGYFK FT DLQTRLKKFVESGQLGPFKNGYWGSKAYKLPPEANLMAVAHYLEALDFQKEIVKIHTIF FT GGKNPHPNWLVGGVPCPINVDGTGAVGAINMERLNLISSIIDRLIEFNEMVYLPDVAAI FT GSFYKDWLYGGGLSGQSVLAYGDVPEHANDYSAKSLKLPRGAIINGNLSEVFPVDHANP FT DEIQEFVVHSWYKYPDETKGLHPWDGVTEPNYVLGPNAKGTKTAIEQLDEGGKYSWIKA FT PRWKGHAMEVGPLARWVVGYAQNKSEFKDPVDKFLRDLNLPTSALFSTLGRTAARALES FT VWAGRQMRYFQDKLVANIKAGDSSTANVDKWKPESWPKEAKGVGFTEAPRGALAHWIKI FT KDTKIDNYQCVVPTTWNGSPRDPKGNIGAFEASLMNTPMVNPEQPLEILRTIHSFDPCL FT ACSTHVMSPDGQELAKVKVR" FT misc_signal 3211..3247 FT /note="pot. transcription termination signal; putative" FT misc_signal 3255..3287 FT /note="pot. transcription termination signal; putative" XX SQ Sequence 3523 BP; 685 A; 1142 C; 1088 G; 608 T; 0 other; cggcccacgt gcggtcgcga tccagcacat cgtgcgctcg ttcgatccct gcatggtgtg 60 accgcgcact gaccggaaac ttcgttgccg gctgcaaatc gaattgtttc cgcttctcga 120 tctccagcat cctcgtgtac cgctcctgcg agcaattcaa acacccaacg ttctggaatc 180 tctccacttt tgaatcgctc caggctgttg gcctgcttct tgctgtcctt ggcgtcatcg 240 aaaagtcaaa gcgaaggcgg gaggatacat gggcgcggcg acggaaacgt tttacagcgt 300 gatcaggcgg caaggcatca cgcgtcggag ctttcacaaa ttttgcagcc tgacggcgac 360 gagcctcggg ctcgggccgc tggcggcgag ccgcatcgcg aacgcgctcg agaccaagcc 420 gcgcgtgccc gtgatctgga tgcacggcct cgaatgcacc tgctgctccg agagcttcat 480 ccggtccgcg cacccgctgg tcaaggatgc cgtgctgtcg atgatctcgc tcgactatga 540 cgacaccatc atggcggccg cgggtcacca ggcggaggcc atcctcgagg agacccgcgc 600 caagcacaaa ggccagtaca tcctcgcggt ggaaggcaat ccgccgctga acgaaggcgg 660 catgttctgc atcgacggcg gcaagccgtt cgtcgagaag ctcaagatga tggccgagga 720 cgcgatggcg atcatcgcct ggggcgcctg cgcgtcctgg ggctgcgtgc aggcggcaaa 780 gcccaatccg acccaggcga cgccgatcga caaggtcatc accaacaagc ccatcatcaa 840 ggtgccggga tgccccccga tcgccgaagt gatgaccggc gtcgtcacct tcatcaccac 900 cttcggcaaa ttgcccgagc tcgaccgcca gggccggccg aagatgttct actcgcagcg 960 cattcacgac aaatgctacc gccgtccgca tttcgacgcc ggccagttcg tcgaggagtg 1020 ggacgacgag gccgcccgca agggctactg cctctacaag atgggctgca agggcccgac 1080 cacttacaac gcctgttcga ccgtgcgctg gaacggcggc gtgtccttcc caatccagtc 1140 gggccacggc tgcatcggct gctccgagga cggcttctgg gacaagggct cgttctacga 1200 ccgtctcacc aacatcaagc agttcggcat cgagaagaac gccgatcaga tcggcatggt 1260 ggcggcgggc gccgtcggcg ccgcggtggc cgcgcatgcg gccgtgaccg cagtgaagcg 1320 gctcgcgacc aagcgcgaag acgccgacca caacagctga gcgaagagaa acactagggt 1380 aggaacacaa tgggtatcca gactcccaac ggcttcaatc tcgacaattc cggcaagcgc 1440 atcgtcgtcg atccggtgac ccgtatcgaa ggtcacatgc gggtcgaggt caatgtcgac 1500 gccgacaacg tgatccgcaa tgcggtctcg accggcacga tgtggcgtgg gatcgaagtg 1560 atcctgaaga accgcgatcc ccgcgacgcc tgggcgttca ccgagcggat ctgcggcgtc 1620 tgcaccggca cccacgcact gacgtcggtg cgcgcggtcg agaacgcgct cgggatcacc 1680 attccggaga acgccaattc gatccgcaat ctgatgcagc ttgcattgca ggtgcacgac 1740 cacgtcgtgc atttctatca ccttcacgcg ctggactggg tcgacgtcgt ctccgcgctc 1800 tcggccgatc cgcgggcgac ctcgacgctg gcgcagtcga tctcgaactg gccgctgtcg 1860 tcgcccggct atttcaagga tctgcagaca aggctgaaga agttcgtcga atccgggcag 1920 ctcggtccgt tcaagaacgg ttattggggc agcaaggcct acaagctgcc gcccgaggcc 1980 aatctgatgg ccgtggcgca ttatctggag gcgctcgact tccagaagga gatcgtcaag 2040 atccacacca tcttcggcgg caagaacccg catccgaact ggctggtcgg cggcgtgccc 2100 tgtccgatca acgtcgacgg cacgggtgcc gtcggcgcca tcaacatgga gcggttgaac 2160 ctgatctcct cgatcatcga ccggctgatc gagttcaacg aaatggtcta tctgcccgac 2220 gtggcggcga tcgggtcgtt ctacaaggac tggctctatg gcggcggcct ctcgggccag 2280 agcgtgctcg cctatggcga cgtgcccgaa catgccaacg actattccgc caagagcctg 2340 aagctgccgc ggggcgccat catcaacggc aatctgtccg aagtgtttcc ggtcgatcac 2400 gccaaccccg acgagatcca ggagttcgtg gtccactcct ggtacaaata ccctgacgag 2460 accaagggcc tgcatccctg ggacggcgtc accgagccga actacgtgct ggggcccaat 2520 gcgaagggca ccaagaccgc gatcgagcag ctcgacgagg gcggcaagta ttcgtggatc 2580 aaggcacctc gctggaaggg ccacgccatg gaggtcggcc cgctggcgcg ctgggtggtc 2640 ggctacgcgc agaacaaatc cgagttcaag gatcccgtcg acaagttcct gagggatttg 2700 aacctgccga cgtcggcgct gttctcgacg ctcggccgca ccgccgcgcg cgcgctcgaa 2760 tccgtctggg ccggacggca gatgcgctac ttccaggaca agctcgtcgc caacatcaag 2820 gccggggaca gctcgaccgc caatgtcgac aagtggaagc cggagagctg gccgaaggaa 2880 gccaagggcg tcggcttcac cgaggcgccg cgcggcgcgc tcgcgcactg gatcaagatc 2940 aaggacacca agatcgacaa ctaccagtgc gtcgtgccga ccacctggaa cggctcgccg 3000 cgcgatccca agggcaacat cggcgccttc gaggcgtcgc tgatgaacac gccgatggtc 3060 aatcccgagc agccgctcga gattctgcgc acgatccatt ccttcgatcc gtgcctggcc 3120 tgttcgacgc acgtcatgag cccggatggc caggagctcg ccaaggtcaa ggtcaggtag 3180 gagggcgcca tgatggatgc agttgctccc gcctccgacg cgaggcccga cctcgcatcg 3240 cgcgacgctt ccggagaacg tgcggtgggg cggcccaccg tctatgtcta cgaagcgccg 3300 gtgcggatct gccactgggt gaacgcgttc tcgatcatcg tgctgatggt gaccggctat 3360 ctgatcggaa cgccgctgcc aacggtcgcg ggcgaggcgt ccgacaactt cgtgatgggc 3420 tacattcgct tcgcccattt cgctgcggga caggtgctcg ccgtgttctt cctcacgcgt 3480 atcctgtggg ccttcgtcgg caaccatcac tcccggcaga tct 3523 // |
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