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EBI DbfetchID J03479; SV 1; linear; genomic DNA; STD; PRO; 3712 BP. XX AC J03479; M17729; XX DT 16-JUL-1988 (Rel. 16, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE S.aureus enzyme III-lac (lacF), enzyme II-lac (lacE), and DE phospho-beta-galactosidase (lacG) genes, complete cds. XX KW enzyme II-lac; enzyme III-lac; phospho-beta-galactosidase; KW phosphotransferase system. XX OS Staphylococcus aureus OC Bacteria; Firmicutes; Bacillales; Staphylococcus. XX RN [1] RP 1920-3712 RX PUBMED; 3111370. RA Breidt F.Jr., Stewart G.C.; RT "Nucleotide and deduced amino acid sequences of the Staphylococcus aureus RT phospho-beta-galactosidase gene"; RL Appl. Environ. Microbiol. 53(5):969-973(1987). XX RN [2] RP 1-2152 RX PUBMED; 2824493. RA Breidt F.Jr., Hengstenberg W., Finkeldei U., Stewart G.C.; RT "Identification of the genes for the lactose-specific components of the RT phosphotransferase system in the lac operon of Staphylococcus aureus"; RL J. Biol. Chem. 262(34):16444-16449(1987). XX CC Draft entry and computer-readable copy for [1],[2] kindly provided CC by G.C.Stewart, 09-SEP-1987. XX FH Key Location/Qualifiers FH FT source 1..3712 FT /organism="Staphylococcus aureus" FT /mol_type="genomic DNA" FT /db_xref="taxon:1280" FT CDS 102..413 FT /codon_start=1 FT /transl_table=11 FT /note="enzyme III-lac (lacF)" FT /db_xref="GOA:P0A0D6" FT /db_xref="HSSP:2E2A" FT /db_xref="InterPro:IPR003188" FT /db_xref="UniProtKB/Swiss-Prot:P0A0D6" FT /protein_id="AAA26648.1" FT /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIEE FT GNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKRG" FT CDS 413..2131 FT /codon_start=1 FT /transl_table=11 FT /note="enzyme II-lac (lacE)" FT /db_xref="GOA:P11162" FT /db_xref="InterPro:IPR004801" FT /db_xref="UniProtKB/Swiss-Prot:P11162" FT /protein_id="AAA26649.1" FT /translation="MTMMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSS FT IFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN FT QINFISTMQAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKRN FT ITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTA FT ADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITS FT GTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAPLVLN FT PVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAI FT TLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSD FT DAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEY FT QLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQQFEN" FT CDS 2149..3561 FT /codon_start=1 FT /transl_table=11 FT /note="phospho-beta-galactosidase (lacG)" FT /db_xref="GOA:P11175" FT /db_xref="HSSP:1PBG" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P11175" FT /protein_id="AAA26650.1" FT /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYT FT AEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAECH FT KRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNEIGP FT IGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHALPTKY FT PFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDY FT AILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQREFDVDV FT PRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDY FT VRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAY FT WYKELAETKEIK" XX SQ Sequence 3712 BP; 1269 A; 548 C; 749 G; 1146 T; 0 other; gtacaactgg tttcaaaaat attgatgact taaataaagt attaaaagat actgcgacat 60 cttggaaaca aagaaaataa gtttaaaagg ggattaatgc tatgaataga gaagaagtcc 120 aattattagg ttttgaaatt gttgcatttg caggggatgc acgttctaag tttttagaag 180 cattaacagc agctcaagct ggagattttg caaaagcaga tgcactgatt gaagaaggaa 240 acaattgcat tgcggaagcg catagagcac aaacaagcct gttggctaaa gaagcgcaag 300 gtgatgatat tgcatacagt gtaacgatga tgcatggtca agatcattta atgacaacaa 360 ttttactgaa agatttaatg aagcatttat tagaatttta taaaagaggg tgatgacaat 420 gatgcaaaaa ttgatcgcac aaattgaaaa agggaagcca tttttcgaga aattatcacg 480 taacatatat ttaagagcca tccgtgatgg atttatttct gcaatgcctg tcatcttatt 540 ctcaagtatc tttctattaa ttgcatatgt gccaaatata tttggtttta aatgggataa 600 aggcatggaa gccattttaa tgaaaccata taactatacg atggggcttg tagcattttt 660 agtagcagga acgacagcga aatctttaac agattcattt aaccgtaagc tagaaagtac 720 aaatcaaatt aactttattt ctacaatgca agcagcgatg tgtggattct tgttcttagc 780 ttctgatcca gccaaagatg gtggattctt aagtgcattt atgggaacta aaggtttatt 840 aacagcattc ttatcagcat ttgtaactgt tattgtttat aacttctgtg tgaagcgcaa 900 tattacaatt aaaatgccga aagaagtacc gccgaatatt tcacaagtat ttaaggactt 960 aattccattt tcagcggtaa tcatcattct ttatgcatta gatttagtca ttcgcaacag 1020 ctttaaatca aatgtagcgg aaggtatttt aaaattattc gaaccattat ttacagcagc 1080 agatggatgg attggtgtca caattatctt tggtgccttt gcattattct ggtttgtagg 1140 tattcatggt ccgtcaattg tagagccagc aattgcagcc attacatatg cgaatatcga 1200 agcgaacttc aagttgcttc aagctggaga acacgcagat aaaattatta catcaggtac 1260 acaaatgttt atcgttacat ttggaggtac gggtgcaaca ttggtcgtcc cgttcatgtt 1320 tatgtggatg acgaaatcta aacgaaacaa agcgattgga cgtgcatcag ttgtaccaac 1380 attctttggt gtaaacgaac caattttatt tggtgcacca ctagtattga atcctgtgtt 1440 ctttattcca tttgtattag caccaattgt taacgtatgg attttcaaac ttttcgttga 1500 agtgttagga atgaatagtt ttagtgtgaa tttaccttgg acaacaccag gtccattggg 1560 cattatcatg ggtacaggtt ttggtttatg gtcattcgta ctagctatta ctttgattgt 1620 tgtagatatt attatttact acccattcct aaaagtttat gatagtgaaa ttcttgatga 1680 agaagaagga cgtaaagaaa gtaattcaga tttaaaagaa aaagttgcag caaactttga 1740 tacgaaaaaa gctgattcaa ttttagcggc aagtggtgta tcagacgatg cagctaaagc 1800 ttcaaatatc acagaacaaa caaatgtctt agtactttgt gccggtggtg gtacaagtgg 1860 attattagcc aatgcactta ataaagcagc tgaagaatat catgtgccag tgaaagcggc 1920 agctggcgga tatggcgcac atatggacat tatgaaagag taccaattaa tcattttagc 1980 gccacaagtt gcgtctaact atgaagatat taagcaagat acagatagat taggtattaa 2040 attagcgaag actcaaggtg ctgaatatat caaattgaca cgagatggcc aagcagccct 2100 tgatttcgtt caacaacaat ttgaaaatta agaataggag tttttcatat gactaaaaca 2160 ttacctgaag attttatttt tggtggagca acagcagcat atcaagcaga aggtgcgaca 2220 aatacggatg gtaaaggtcg cgtagcatgg gatacgtatt tagaagaaaa ctattggtac 2280 actgcagaac cagcgagtga tttttataac agatatccag tggatttaga attaagtgaa 2340 aaattcggtg ttaatggtat ccgaatttca attgcatggt ctcgtatctt cccaaatgga 2400 tatggcgaag ttaaccctaa aggtgttgag tattaccata agttatttgc agagtgtcat 2460 aaacgtcatg ttgaaccatt tgtaacattg catcattttg atacaccaga agtgttacac 2520 aaagatggcg attttctaaa tcgtaaaacg attgactact ttgtagatta tgctgaatat 2580 tgttttaaag aattcccaga agtaaaatat tggactacat tcaatgaaat tggacctatt 2640 ggtgatggtc aatatttagt aggtaaattc cctccaggta ttaaatatga ttttgaaaaa 2700 gtattccaat cacatcataa catgatggtg gctcatgcta gagcagtaaa attatttaaa 2760 gatggcggat acaaaggaga aattggtgtt gtacacgcat tgccaacaaa atatccgttc 2820 gatccatcaa atcctgaaga tgttcgtgca gcagaattag aagatatcat ccataataaa 2880 ttcatcttag atgcaacata tttaggtaag tattctcgtg aaacgatgga aggtgtgcaa 2940 catatcttat ctgttaatgg cggtaaatta aacattacag atgaagatta tgccatttta 3000 gatgcagcca aagatttaaa cgacttctta ggtatcaatt actacatgag tgattggatg 3060 agaggttacg atggagaatc tgaaatcacg cataatgcga caggtgataa aggcggttct 3120 aaataccaac ttaaaggtgt cggacaacgt gaatttgatg tcgacgtacc tcgcacagac 3180 tgggactgga tgatctatcc tcaaggttta tatgatcaaa tcatgcgtgt tgttaaagat 3240 tatcctaact atcataagat ttatatcact gaaaatggtt taggatataa agatgaattt 3300 attgaatctg aaaaaacagt tcatgatgat gcacgtattg attatgtaag acaacatttg 3360 aacgttattg cagatgctat tatagatggt gcaaatgtta aaggttactt tatttggtca 3420 ttaatggatg tgttctcttg gtcaaatggt tatgaaaaac gatatggttt attctatgtc 3480 gattttgaga ctcaagaacg ttatcctaag aaaagtgctt attggtacaa agagttagcc 3540 gaaacaaaag aaattaaata attatagata ttaaaaggcc tatcagtcga gtgctggtag 3600 gtctttattt atagaaagag ataaattaaa catgcatata catatttata tattaatcaa 3660 tgggtgacac accattgaaa gctaaagaag caatcaatta acaaacgtta ac 3712 // |
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