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EBI DbfetchID J02914; SV 1; linear; genomic DNA; STD; PRO; 3903 BP. XX AC J02914; XX DT 24-JUL-1990 (Rel. 24, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE M.thermoautotrophicum hydroxy-5-deazaflavin-reducing hydrogenase (frhADGB) DE gene, complete cds. XX KW 8-hydroxy-5-deazaflavin-reducing hydrogenase. XX OS Methanothermobacter thermautotrophicus OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; OC Methanobacteriaceae; Methanothermobacter. XX RN [1] RP 1-3903 RX DOI; 10.1021/bi00483a011 RX PUBMED; 2207102. RA Alex L.A., Reeve J.N., Orme-Johnson W.H., Walsh C.T.; RT "Cloning, sequence determination, and expression of the genes encoding the RT subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing RT hydrogenase from Methanobacterium thermoautotrophicum delta H"; RL Biochemistry 29(31):7237-7244(1990). XX CC Draft entry and computer-readable sequence for [Biochemistry (1990) CC In press] kindly submitted CC by L.A.Alex, 25-MAY-1990. XX FH Key Location/Qualifiers FH FT source 1..3903 FT /organism="Methanothermobacter thermautotrophicus" FT /mol_type="genomic DNA" FT /db_xref="taxon:145262" FT RBS 284..293 FT /note="ribosomal binding site (put.); putative" FT CDS 298..1515 FT /codon_start=1 FT /transl_table=11 FT /note="F420 hydrogenase alpha-subunit (frhA)" FT /db_xref="GOA:P19496" FT /db_xref="HSSP:1CC1" FT /db_xref="InterPro:IPR018194" FT /db_xref="UniProtKB/Swiss-Prot:P19496" FT /protein_id="AAA72187.1" FT /translation="MSERIVISPTSRQEGHAELVMEVDDEGIVTKGRYFSITPVRGLEK FT IVTGKAPETAPVIVQRICGVCPIPHTLASVEAIDDSLDIEVPKAGRLLRELTLAAHHVN FT SHAIHHFLIAPDFVPENLMADAINSVSEIRKNAQYVVDMVAGEGIHPSDVRIGGMADNI FT TELARKRLYARLKQLKPKVDEHVELMIGLIEDKGLPKGLGVHNQPTLASHQIYGDRTKF FT DLDRFTEVMPESWYDDPEIAKRACSTIPLYDGRNVEVGPRARMVEFQGFKERGVVAQHV FT ARALEMKTALARAIEILDELDTSAPVRADFDERGTGKLGVGAIEGPRGLDVHMAQVENG FT KIQFYSALVPTTWNIPTMGPATEGFHHEYGPHVIRAYDPCLSCATHVMVVDDEDRSVIR FT DEMVRL" FT RBS 1514..1520 FT /note="ribosomal binding site (put.); putative" FT CDS 1524..2000 FT /codon_start=1 FT /transl_table=11 FT /note="F420 hydrogenase delta-subunit (frhD)" FT /db_xref="GOA:P19497" FT /db_xref="InterPro:IPR000671" FT /db_xref="UniProtKB/Swiss-Prot:P19497" FT /protein_id="AAA72188.1" FT /translation="MPYDAEILVVGCGNILFKDDGFGPEVIKALEEYFKDREKPDNVMF FT IDAGTGGPHFVFSLPHEEWKKMIVVDVVEFNAEPGTLRKFDVTEIPKGSYENMHTWPVS FT QPLHELSEKIDVVVIGCKPKEISAPNVEMGLTPPVKKAIPRAIQMILDEIGVSK" FT RBS 2100..2106 FT /note="ribosomal binding site (put.); putative" FT CDS 2114..2824 FT /codon_start=1 FT /transl_table=11 FT /note="F420 hydrogenase gamma-subuint (frhG)" FT /db_xref="GOA:P19498" FT /db_xref="InterPro:IPR006137" FT /db_xref="UniProtKB/Swiss-Prot:P19498" FT /protein_id="AAA72189.1" FT /translation="MAEENAKPRIGYIHLSGCTGDAMSLTENYDILAELLTNMVDIVYG FT QTLVDLWEMPEMDLALVEGSVCLQDEHSLHELKELREKAKLVCAFGSCAQTGCFTRYSR FT GGQQAQPSHESFVPIADLIDVDLAIPGCPPSPEIIAKAVVALLNNDMEYLQPMLDLAGY FT TEACGCDLQTKVVNQGLCTGCGTCAMACQTRALDMTNGRPELNSDRCIKCGICYVQCPR FT SWWPEEQIKKELGL" FT RBS 2823..2832 FT /note="ribosomal binding site (put.); putative" FT CDS 2837..3682 FT /codon_start=1 FT /transl_table=11 FT /note="F420 hydrogenase beta-subunit (frhB)" FT /db_xref="GOA:P19499" FT /db_xref="InterPro:IPR007525" FT /db_xref="UniProtKB/Swiss-Prot:P19499" FT /protein_id="AAA72190.1" FT /translation="MVLGTYKEIVSARSTDREIQKLAQDGGIVTGLLAYALDEGIIEGA FT VVAGPGKEFWKPEPMVAMTSDELKAAAGTKYTFSPNVLMLKKAVRQYGIEKLGTVAIPC FT QTMGIRKAQTYPFGVRFVADKIKLLVGIYCMENFPYTSLQTFICEKLGLNMELVEKMDI FT GKGKFWVYTQDDVYTLPLKETHGYEQAGCKICKDYVAELADVSTGSVGSPDGWSTVITR FT TDSGDSIFKQAVEAGIFETKPIEEVKPGLGLLEKLSAQKKEKAEKNIAARKEMGLPTPY FT " XX SQ Sequence 3903 BP; 1201 A; 878 C; 965 G; 859 T; 0 other; ctgcagcaac agctccaatt atcaggacta tcgccaccga tgctagaacc tttgagttta 60 tcatcaactc accttcctgg tgttaacaac acatttagta tgtgggtcaa gatatataac 120 actcagtatt actgaacaaa aaattaatat tataaactct gttttctact aataataagt 180 gatctataaa atgatataaa tagttttcac tttttttata attgaaaaac ttataacatt 240 taaaaatagt tataatattg aaggtttttt gaactttaaa aacaagaggt gatacatttg 300 agcgaaagga ttgttatatc gccgacatca cgacaagaag gacatgcaga acttgtcatg 360 gaagtcgatg atgagggaat cgtgacaaag gggcgatact tcagtattac tcctgtcagg 420 ggccttgaga aaatagtgac aggtaaagca cctgaaacag cacctgtcat cgtccagagg 480 atatgtggag tatgccctat accacacacc ctggcttccg ttgaggcaat agacgactcc 540 cttgacattg aggttccaaa ggccggaaga cttctccgtg aacttacact tgcagcacac 600 cacgtaaaca gccacgcaat acatcacttc ctcatagcac cggacttcgt gcctgaaaac 660 ctcatggcag acgccataaa ctcagtctcc gagataagga aaaacgcaca gtacgtagtt 720 gacatggttg caggtgaggg tatacaccca tccgatgtca ggataggtgg aatggccgac 780 aacataacgg aactcgcaag gaaaaggctg tatgcgaggt taaagcagct caaaccaaaa 840 gtcgacgaac acgttgaact catgatagga ttaatcgagg ataagggcct tccaaagggc 900 cttggagtcc acaaccagcc aacacttgca agccaccaga tctacggtga caggacaaaa 960 ttcgatctgg acaggttcac cgaggtaatg cctgagagct ggtacgatga ccctgaaata 1020 gccaagaggg cctgctcaac aataccactc tacgatggaa gaaacgtgga ggtcggtcca 1080 agagcaagga tggttgaatt ccagggattc aaggagaggg gtgttgttgc acagcacgtt 1140 gcaagggcac ttgagatgaa aacggcactc gcaagggcaa ttgaaatcct tgatgaactg 1200 gacacatccg caccggtaag ggcagacttc gatgagagag gcacaggtaa actgggtgta 1260 ggtgcaattg aaggtccaag gggactggac gttcacatgg cccaggttga aaatggtaag 1320 atacagttct acagtgcact cgtcccaaca acctggaaca tcccaacaat gggtcctgca 1380 actgaaggat tccaccatga atacgggcca cacgtcatac gcgcttacga cccctgtctc 1440 tcatgcgcaa cacacgtgat ggttgttgat gatgaggaca gatcagtaat cagggatgaa 1500 atggtcagac tctagggaat aacatgccat acgacgctga gattctagtg gtgggctgtg 1560 gaaacatcct tttcaaggac gatggattcg gcccagaggt tatcaaggcc cttgaagagt 1620 acttcaagga tagggaaaaa ccagataatg ttatgttcat tgatgctggg actggcggtc 1680 cgcactttgt cttctccctc ccccatgagg agtggaagaa gatgatagtt gtggatgttg 1740 ttgaattcaa cgcagaaccc ggaacactca ggaaattcga tgtcactgag attccaaagg 1800 gatcctatga aaatatgcat acatggccag tgagccagcc cctccatgaa ctcagtgaaa 1860 agatcgatgt tgtggtaata gggtgtaaac ccaaggagat atcagctccc aatgtggaaa 1920 tgggcctcac acccccagta aaaaaggcta ttcccagagc cattcagatg atcttagatg 1980 agattggggt ttctaaatga gcttaattgc ccgcatcaaa agatttttag gattggaggc 2040 tgaagctaag agggaagaac ccgaaaaaga aaaatcggaa cctgttggag cttcaaaaga 2100 ggaggttgaa aaagtggctg aagaaaatgc aaaaccaaga ataggttaca ttcacctcag 2160 tggatgtacc ggagatgcca tgtcgttaac tgaaaattac gacattctag cagaattact 2220 caccaacatg gtggacatag tatacggaca gaccctggtg gatctctggg agatgccaga 2280 gatggatctg gcccttgttg agggatctgt ctgtctgcag gacgaacaca gcctgcacga 2340 actcaaagaa ctgagggaga aggcaaaact cgtctgcgcc ttcggttcat gcgcacagac 2400 aggctgcttc acaaggtact caaggggcgg acagcaggca cagccatcac acgagtcctt 2460 tgtaccaata gcagacctca tagatgtgga cctcgccatt ccagggtgcc caccatcacc 2520 tgagataata gcaaaggcag tcgttgcact cctcaacaat gacatggagt acctccagcc 2580 aatgctggac cttgcaggct acacagaggc atgcggatgc gacctccaga caaaggttgt 2640 aaaccagggt ctctgcactg gatgtggaac atgtgcaatg gcctgccaga caagggccct 2700 tgacatgacc aacggaagac ccgaactcaa cagcgaccgc tgtataaaat gtggaatctg 2760 ctatgtgcag tgcccaagaa gctggtggcc agaagaacag atcaaaaagg agttagggct 2820 ataggaggct ggaaaaatgg ttttaggtac ttacaaggaa atagtttccg ccagatcaac 2880 tgacagagag attcagaaat tagcccagga tggaggaata gttacaggtc ttctagcata 2940 tgcccttgac gagggcataa tcgaaggcgc agttgttgca ggacccggaa aggagttctg 3000 gaagccagaa ccaatggtcg ccatgacctc agatgaactc aaggcggctg caggtaccaa 3060 gtacacattc tcaccaaacg tcctgatgct caagaaggca gtgaggcagt acggtataga 3120 gaaactgggt acagttgcaa taccctgcca gaccatgggt ataaggaagg cccagaccta 3180 cccattcggt gtcaggtttg ttgcagataa gataaaactc ctggtcggta tctactgcat 3240 ggagaacttc ccatacacat cactgcagac cttcatctgt gaaaaactcg gattaaacat 3300 ggagctcgtt gagaagatgg acataggtaa aggaaaattc tgggtctaca cccaggacga 3360 tgtctacaca ctccccctca aggagaccca tggatacgaa caggcaggat gcaagatctg 3420 caaggactat gtggctgagc tggcagacgt atcaacaggt tcagtgggat caccggatgg 3480 ctggtcaaca gtcataacaa ggaccgattc aggggactca atattcaagc aggctgttga 3540 ggcaggtata ttcgagacca aaccaataga ggaagtcaaa cctggcctgg gacttcttga 3600 aaaactctct gcacagaaga aggaaaaggc agagaagaac atcgctgcaa ggaaagagat 3660 gggattacca acaccatact gatcccatcc ctaaattttc tatttttcag aacattcagt 3720 ccattcaact gcatcctcaa gttaactatt ctgttataac cgtgcagccg tcctcctcaa 3780 caatgactgt atgttcccac tgggcgacca ttgcaccgct cttttccctc agcacatggt 3840 aggggtatat ggcccttgac tgtatcagca ttctcatgga ggcattcagt ctctttgcat 3900 cga 3903 // |
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