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EBI DbfetchID J02732; SV 1; linear; genomic DNA; STD; PRO; 13339 BP. XX AC J02732; K00047; XX DT 19-APR-1990 (Rel. 23, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE B.subtilis pur operon encoding purine biosynthesis enzymes, 12 genes. XX KW adenylosuccinate lyase; inosine monophosphate cyclohydrolase; KW phosphoribosyl aminoimidazole carboxylase; KW phosphoribosyl aminoimidazole succinocarboxamide synthetase; KW phosphoribosyl aminoimidazole synthetase; KW phosphoribosyl glycinamide carboxymide formyltransferase; KW phosphoribosylformyl glycinamidine synthetase; KW phosphoribosylpyrophosphate amidotransferase. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 7176-8826 RX PUBMED; 6411717. RA Makaroff C.A., Zalkin H., Switzer R.L., Vollmer S.J.; RT "Cloning of the Bacillus subtilis glutamine phosphoribosylpyrophosphate RT amidotransferase gene in Escherichia coli. Nucleotide sequence RT determination and properties of the plasmid-encoded enzyme"; RL J. Biol. Chem. 258(17):10586-10593(1983). XX RN [2] RP 1-13339 RX PUBMED; 3036807. RA Ebbole D.J., Zalkin H.; RT "Cloning and characterization of a 12-gene cluster from Bacillus subtilis RT encoding nine enzymes for de novo purine nucleotide synthesis"; RL J. Biol. Chem. 262(17):8274-8287(1987). XX RN [3] RP 146-471 RX PUBMED; 3134353. RA Ebbole D.J., Zalkin H.; RT "Detection of pur operon-attenuated mRNA and accumulated degradation RT intermediates in Bacillus subtilis"; RL J. Biol. Chem. 263(22):10894-10902(1988). XX DR GOA; P12049. DR InterPro; IPR003850; FGAM_PurS. DR PDB; 1T4A; X-ray. DR RFAM; RF00167; Purine. DR UniProtKB/Swiss-Prot; P12049; YEXA_BACSU. XX CC Draft entry and computer-readable sequence for [2] kindly provided CC by D.J.Ebbole, 13-APR-1987. CC The -10 and -35 regions are located at positions 164-169 and CC 187-192. An antiterminator is found at 276-373 and terminators at CC 347-394, 8711-8737 and 13246-13339. Dyad symmetries are positioned CC at 3346-3378 and 3365-3419. XX FH Key Location/Qualifiers FH FT source 1..13339 FT /organism="Bacillus subtilis" FT /strain="W168" FT /sub_strain="PY79" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT mRNA 197..>927 FT /note="PUR mRNA" FT misc_feature 276..373 FT /note="antiterminator dyad" FT misc_feature 377..396 FT /note="terminator dyad" FT CDS 439..927 FT /codon_start=1 FT /transl_table=11 FT /note="phosphoribosyl aminoimidazole carboxylase I (PUR-E)" FT /db_xref="GOA:P12044" FT /db_xref="HSSP:1O4V" FT /db_xref="InterPro:IPR000031" FT /db_xref="UniProtKB/Swiss-Prot:P12044" FT /protein_id="AAA22674.1" FT /translation="MQPLVGIIMGSTSDWETMKHACDILDELNVPYEKKVVSAHRTPDF FT MFEYAETARERGIKVIIAGAGGAAHLPGMTAAKTTLPVIGVPVQSKALNGMDSLLSIVQ FT MPGGVPVATTSIGKAGAVNAGLLAAQILSAFDEDLARKLDERRENTKQTVLESSDQLV" FT CDS 920..2059 FT /codon_start=1 FT /transl_table=11 FT /note="phosphoribosyl aminoimidazole carboxylase II (PUR-K; FT ttg start codon)" FT /db_xref="GOA:P12045" FT /db_xref="HSSP:1B6R" FT /db_xref="InterPro:IPR013817" FT /db_xref="UniProtKB/Swiss-Prot:P12045" FT /protein_id="AAA22675.1" FT /translation="MSKQIIYPGAVIGIIGGGQLGKMMAVSAKQMGYKVAVVDPVKDSP FT CGQVADVEITAHYNDREAIRKLAEISDIITYEFENIDYDALHWLKDHAYLPQGSELLLI FT TQNRETEKKAIQSAGCEVAPYSIVKTKNELKQAVQELRLPAVLKTCRGGYDGKGQFVIK FT EEAQMEQAAALLEHGTCILESWVSFKMELSVIVVRSVNGEISTFPTAENIHHNNILFQS FT IVPAVEKGIQQKAADLAVKLADELNLVGPLAVEMFLTEDGELLVNELAPRPHNSGHYTL FT DLCETSQFEQHIRAVCGLPLGKTDLLKPGMMVNLLGDEVKLVEEDPELLKEAKLYIYGK FT HEIKKGRKMGHITFMKQPEDEWIQEITNKWMNRDGGQAE" FT CDS 2056..3351 FT /codon_start=1 FT /transl_table=11 FT /note="adenylosuccinate lyase (PUR-B)" FT /db_xref="GOA:P12047" FT /db_xref="InterPro:IPR004769" FT /db_xref="PDB:1F1O" FT /db_xref="UniProtKB/Swiss-Prot:P12047" FT /protein_id="AAA22676.1" FT /translation="MIERYSRPEMSAIWTDENRFQAWLEVEILACEAWAELGVIPKEDV FT KVMRENASFDINRILEIEKDTRHDVVAFTRAVSESLGEERKWVHYGLTSTDVVDTALSY FT LLKQANDILLKDLERFVDIIKEKAKEHKYTVMMGRTHGVHAEPTTFGLKLALWHEEMKR FT NLERFKQAKAGIEVGKISGAVGTYANIDPFVEQYVCEKLGLKAAPISTQTLQRDRHADY FT MATLALIATSIEKFAVEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSENMTGMA FT RVIRGYMMTAYENVPLWHERDISHSSAERIILPDATIALNYMLNRFSNIVKNLTVFPEN FT MKRNMDRTLGLIYSQRVLLALIDTGLTREEAYDTVQPKAMEAWEKQVPFRELVEAEEKI FT TSRLSPEKIADCFDYNYHLKNVDLIFERLGLA" FT CDS 3424..4149 FT /codon_start=1 FT /transl_table=11 FT /note="phosphoribosyl aminoidazole succinocarboxamide FT synthetase (PUR-C; gtg start codon)" FT /db_xref="GOA:P12046" FT /db_xref="HSSP:1KUT" FT /db_xref="InterPro:IPR018236" FT /db_xref="UniProtKB/Swiss-Prot:P12046" FT /protein_id="AAA22677.1" FT /translation="MNIVKNELLYEGKAKKIYKTDDENTLYVVYKDSATAFNGEKKAEI FT SGKGRLNNEISSLIFKHLHAKGINNHFIERISETEQLIKKVTIVPLEVVVRNVVAGSMS FT KRLGIPEGTELEQPIIEFYYKDDALGDPLITEDHIWLLKAATPEQVETIKSITTIVNEE FT LQSIFDDCHVRLIDFKLEFGLDAEGQVLLADEISPDTCRLWDKETNEKLDKDLFRRNLG FT SLTDAYEEIFNRLGGIHHV" FT CDS 4393..5076 FT /codon_start=1 FT /transl_table=11 FT /note="phosphoribosylformyl glycinamidine synthetase I FT (PUR-L; gtg start codon)" FT /db_xref="GOA:P12041" FT /db_xref="InterPro:IPR010075" FT /db_xref="UniProtKB/Swiss-Prot:P12041" FT /protein_id="AAA22678.1" FT /translation="MKFAVIVLPGSNCDIDMYHAVKDELGHEVEYVWHEETSLDGFDGV FT LIPGGFSYGDYLRCGAIARFANIMPAVKQAAAEGKPVLGVCNGFQILQELGLLPGAMRR FT NKDLKFICRPVELIVQNDETLFTASYEKGESITIPVAHGEGNFYCDDETLATLKENNQI FT AFTYGSNINGSVSDIAGVVNEKGNVLGMMPHPERAVDELLGSADGLKLFQSIVKNWRET FT HVTTA" FT CDS 5060..7288 FT /codon_start=1 FT /transl_table=11 FT /note="phosphoribosylformyl glycinamidine synthetase II FT (PUR-Q)" FT /db_xref="GOA:P12042" FT /db_xref="InterPro:IPR010074" FT /db_xref="UniProtKB/Swiss-Prot:P12042" FT /protein_id="AAA22679.1" FT /translation="MSLLLEPSKEQIKEEKLYQQMGVSDDEFALIESILGRLPNYTEIG FT IFSVMWSEHCSYKNSKPILRKFPTSGERVLQGPGEGAGIVDIGDNQAVVFKIESHNHPS FT ALEPYQGAATGVGGIIRDVFSMGARPIAVLNSLRFGELTSPRVKYLFEEVVAGIAGYGN FT CIGIPTVGGEVQFDSSYEGNPLVNAMCVGLINHEDIKKGQAKGVGNTVMYVGAKTGRDG FT IHGATFASEEMSDSSEEKRSAVQVGDPFMEKLLLEACLEVIQCDALVGIQDMGAAGLTS FT SSAEMASKAGSGIEMNLDLIPQRETGMTAYEMMLSESQERMLLVIERGREQEIIDIFDK FT YDLEAVSVGHVTDDKMLRLTHKGEVVCELPVDALAEEAPVYHKPSQEPAYYREFLETDV FT PAPQIEDANEMLKALLQQPTIASKEWVYDQYDYMVRTNTVVAPGSDAGVLRIRGTKKAL FT AMTTDCNARYLYLDPEVGGKIAVAEAARNIICSGAEPLAVTDNLNFGNPEKPEILWQIE FT KAADGISEACNVLSTPVIGGNVSLYNESNGTAIYPTPVIGMVGLIEDTAHITTQHFKQA FT GDLVYVIGETKPEFAGSELQKMTEGRIYGKAPQIDLDVELSRQKALLDAIKKGFVQSAH FT DVSEGGLGVAIAESVMTTENLGANVTVEGEAALLFSESQSRFVVSVKKEHQAAFEATVK FT DAVHIGEVTADGILAIQNQDGQQMIHAQTKELERVWKGAIPCLLKSKA" FT CDS 7264..8694 FT /codon_start=1 FT /transl_table=11 FT /note="phosphoribosylpyrophosphate amidotransferase (PUR-F; FT EC 2.4.2.14)" FT /db_xref="GOA:P00497" FT /db_xref="InterPro:IPR000836" FT /db_xref="PDB:1AO0" FT /db_xref="UniProtKB/Swiss-Prot:P00497" FT /protein_id="AAA22680.1" FT /translation="MLAEIKGLNEECGVFGIWGHEEAPQITYYGLHSLQHRGQEGAGIV FT ATDGEKLTAHKGQGLITEVFQNGELSKVKGKGAIGHVRYATAGGGGYENVQPLLFRSQN FT NGSLALAHNGNLVNATQLKQQLENQGSIFQTSSDTEVLAHLIKRSGHFTLKDQIKNSLS FT MLKGAYAFLIMTETEMIVALDPNGLRPLSIGMMGDAYVVASETCAFDVVGATYLREVEP FT GEMLIINDEGMKSERFSMNINRSICSMEYIYFSRPDSNIDGINVHSARKNLGKMLAQES FT AVEADVVTGVPDSSISAAIGYAEATGIPYELGLIKNRYVGRTFIQPSQALREQGVRMKL FT SAVRGVVEGKRVVMVDDSIVRGTTSRRIVTMLREAGATEVHVKISSPPIAHPCFYGIDT FT STHEELIASSHSVGEIRQEIGADTLSFLSVEGLLKGIGRKYDDSNCGQCLACFTGKYPT FT EIYQDTVLPHVKEAVLTK" FT CDS 8796..9836 FT /codon_start=1 FT /transl_table=11 FT /note="phosphoribosyl aminoimidazole synthetase (PUR-M)" FT /db_xref="GOA:P12043" FT /db_xref="HSSP:1CLI" FT /db_xref="InterPro:IPR004733" FT /db_xref="UniProtKB/Swiss-Prot:P12043" FT /protein_id="AAA22681.1" FT /translation="MSEAYKNAGVDIEAGYEAVKRMKKHVERTKRLGVMGSLGGFGGMF FT DLSELSYQKPVLISGTDGVGTKLKLAFSMDKHDTIGVDAVAMCVNDVLAQGAEPLFFLD FT YLAVGKADPVKIEQIVQGVAEGCEQSGSALVGGETAEMPGLYTADEYDIAGFSVGVAEK FT DEIVTGEKIEEGHLLIGLSSSGLHSNGFSLVRKVLLDDAAWDLDTTYEPFERPLGQELV FT EPTRIYVKPVLAAVKSGKIDGMAHVTGGGFIENIPRMLPEGLSAEIDHGSWPIPPIFSF FT LQEYGKLKEEDMFNVFNMGIGFVLAVKEEHLTDVIGTLESHGEKAYLIGRVKKGEGVTF FT GGAALS" FT CDS 9833..10420 FT /codon_start=1 FT /transl_table=11 FT /note="phosphoribosyl glycinamide formyltransferase FT (PUR-N)" FT /db_xref="GOA:P12040" FT /db_xref="HSSP:1MEN" FT /db_xref="InterPro:IPR004607" FT /db_xref="UniProtKB/Swiss-Prot:P12040" FT /protein_id="AAA22682.1" FT /translation="MKKFAVFASGNGSNFEAIVTRLKEENWDASRALLVCDKPQAKVIE FT RAERFHIPSFAFEPKSYENKAAFEQAIIEQLRLHEVELIALAGYMRLIGDTLLQAYGGK FT IINIHPSLLPAFPGIDAVGQAFRAGVKVAGITVHYVDEGMDTGPIIAQKAIEIDEHDTL FT ETIEQRIHKLEHKWYPSVIKQLLGLNNRGEKA" FT CDS 10417..11955 FT /codon_start=1 FT /transl_table=11 FT /note="phosphoribosyl aminoimidazole carboxy formyl FT formyltransferase/inosine monophosphate cyclohydrolase FT (PUR-H(J))" FT /db_xref="GOA:P12048" FT /db_xref="HSSP:1G8M" FT /db_xref="InterPro:IPR011607" FT /db_xref="UniProtKB/Swiss-Prot:P12048" FT /protein_id="AAA22683.1" FT /translation="MTIKRALISVSDKTNLVPFVKELTELGVEVISTGGTKKLLQENGV FT DVIGISEVTGFPEIMDGRLKTLHPNIHGGLLAVRGNEEHMAQINEHGIQPIDLVVVNLY FT PFKETISKEDVTYEEAIENIDIGGPGMLRAASKNHQDVTVIVDPADYSPVLNQIKEEGS FT VSLQKKRELAAKVFRHTAAYDALIADYLTNVVGEKEPEQFTVTFEKKQSLRYGENPHQE FT ATFYQTALPVKGSIAQAEQLHGKELSYNNIKDADAAVQIVREFTEPAAVAVKHMNPCGV FT GTGKTIAEAFDRAFEADKTSIFGGIIALNREVDKATAEALHNIFLEIIIAPSFSQEALD FT VLTAKKNLRLVTLDVSAAVQKEKQLTSVQGGLLIQDLDMHGFDDAEISIPTKREPNEQE FT WEDLKLAWKVVKHVKSNAIVLAKDNMTVGVGAGQMNRVGSAKIAIEQAGEKAKGSALGS FT DAYFPMPDTVEEAAKAGVTAIIQPGGSIRDEDSIKKADEYGIAMVFTGIRHFKH" FT CDS 11971..13239 FT /codon_start=1 FT /transl_table=11 FT /note="phosphoribosyl glycinamide synthetase (PUR-D; gtg FT start codon)" FT /db_xref="GOA:P12039" FT /db_xref="HSSP:1GSO" FT /db_xref="InterPro:IPR013817" FT /db_xref="UniProtKB/Swiss-Prot:P12039" FT /protein_id="AAA22684.1" FT /translation="MNVLIIGKGGREHTLAWKAAQSSLVENVFAAPGNDGMAASAQLVN FT IEESDHAGLVSFAKQNQVGLTIVGPEVPLIEGLVDEFEKAGLHVFGPSKAAAIIEGSKQ FT FAKDLMKKYDIPTAEYETFTSFDEAKAYVQEKGAPIVIKADGLAAGKGVTVAMTEEEAI FT ACLHDFLEDEKFGDASASVVIEEYLSGEEFSLMAFVKGEKVYPMVIAQDHKRAFDGDKG FT PNTGGMGAYSPVPQISEETVRHAVETIVKPAAKAMVQEGRSFTGVLYAGLMLTENGSKV FT IEFNARFGDPETQVVLPRMESDLVQVLLDLLDDKEVDLRWKDTAAVSVVLASEGYPESY FT AKGTPIGSLAAETEQVVVFHAGTKAEGGEFVTNGGRVANVTAFDETFEAARDRVYKAVD FT EIFKPGLFFRKDIGARALKAAQK" XX SQ Sequence 13339 BP; 3897 A; 2831 C; 3507 G; 3104 T; 0 other; gatatctgcc tgtaaggcga atggaagagc ctatttttat ggaaaaaaag gaaaatgggt 60 caattcagat cgttccgtgc gggaaaaaaa tcgtatttga agggaaattg atctaaaaca 120 cgaacattag tagaatgaat ttttgtatcg ttcgataata tcgttgacat tatccatgtc 180 cgttgttaag ataaacatga aatcaaaaca cgacctcata taatcttggg aatatggccc 240 ataagtttct acccggcaac cgtaaattgc cggactatgc aggaaagtga tcgataaaac 300 tgacatggat atatcgcaga agcgaacgac tgacgataca tgtaccatgc ccggtttgta 360 ttgcttcctc ataagtgcaa tgcagagcgg gtatttttta ttttctgaaa acaaaagcat 420 tagaaggtgg ggaacagaat gcagccgcta gtaggaatca tcatgggaag cacttccgat 480 tgggagacaa tgaaacacgc atgcgacata cttgacgaac tcaatgttcc gtacgaaaaa 540 aaggtcgttt ccgctcaccg gacgcctgat ttcatgtttg aatatgctga aactgctaga 600 gaaagaggca tcaaggtgat tattgccggt gccggaggag cggcgcatct gccagggatg 660 acggctgcga aaacaacact gccagtcatt ggagttccgg ttcagtccaa ggcgctgaac 720 ggaatggatt cacttctttc catcgtccaa atgcctggag gcgtgcctgt tgcgacaaca 780 tccatcggca aagcgggtgc tgtgaacgca ggcctgttag cggcgcaaat tttgtcagca 840 tttgacgaag accttgcccg taagctggat gagagaagag aaaatacaaa acagacagtg 900 ttagaaagca gtgatcagct tgtctaaaca aatcatctat ccgggagctg taatcggcat 960 tatcggcggc ggccagcttg ggaaaatgat ggctgtgtcc gccaaacaaa tggggtataa 1020 agtcgcggtc gttgatccgg tgaaagattc gccgtgcggg caggttgcgg atgtcgaaat 1080 taccgctcat tataatgacc gtgaagcgat tcgaaaattg gctgaaatca gcgatatcat 1140 cacatatgag tttgaaaaca tcgactatga tgcgctgcat tggctaaaag atcatgcgta 1200 tctcccgcaa ggaagtgagc tgctgcttat tacccaaaac cgtgaaacag agaaaaaagc 1260 aattcaatcc gcgggctgtg aagtcgcacc gtacagcatc gtcaaaacaa agaatgaatt 1320 gaaacaggca gtacaggagc tcaggcttcc ggcagtgctg aaaacatgcc gcggcgggta 1380 cgacggcaaa ggccaatttg tgataaaaga agaggcgcaa atggagcagg cagccgctct 1440 gttagaacac ggaacttgca ttcttgaaag ctgggtttct tttaaaatgg aactgtcggt 1500 gatcgtcgtt agatcggtaa acggtgaaat ttcaacattt ccgacagctg aaaacattca 1560 ccacaacaat attcttttcc aaagcatcgt gcccgcagta gagaaaggga ttcagcagaa 1620 ggctgctgat ctcgcagtta agcttgcaga tgagcttaac cttgtcggac cgcttgctgt 1680 tgagatgttc ctgacagagg acggagagct tttggtcaat gaactggcgc caagaccgca 1740 caattcaggg cattatacgc tggacctttg cgagacgagc cagtttgagc agcatatcag 1800 agcggtatgc gggcttccgc ttgggaagac agatttgctg aagccgggca tgatggtgaa 1860 tcttctcggc gatgaagtga agcttgttga ggaagacccg gagcttttaa aagaggcaaa 1920 gctatatata tacggaaaac atgaaatcaa aaaaggccgc aaaatggggc atattacatt 1980 tatgaagcag cctgaagacg aatggattca ggagatcaca aataaatgga tgaatagaga 2040 cggaggacaa gcagaatgat cgaacgttat tcaagacctg aaatgtccgc gatttggacg 2100 gatgaaaaca gatttcaagc atggttagag gttgaaattc ttgcctgtga agcgtgggcg 2160 gagcttggcg tcattccgaa agaagatgta aaggttatgc gcgaaaacgc gtcatttgac 2220 atcaaccgca ttttagaaat cgaaaaggac acgcgccatg acgttgtcgc ttttacgcgc 2280 gctgtatccg aatcactggg cgaagaaaga aaatgggtgc attacggctt aacgtcaact 2340 gacgttgttg atacggctct ttcctactta ttaaaacagg caaacgatat tttgctcaag 2400 gatcttgaga gatttgttga cattataaaa gaaaaagcga aagaacataa atacacagtc 2460 atgatggggc gcacacacgg cgtacacgct gagcctacaa cattcggctt aaaacttgcg 2520 ctttggcatg aagaaatgaa acgtaatctt gagcgcttca aacaagcgaa agcaggcatc 2580 gaggttggaa agatttccgg tgctgtcggc acatatgcga acattgatcc atttgttgaa 2640 caatatgtct gtgagaagct cggattgaaa gcagcaccga tttccactca aacccttcag 2700 cgtgaccgcc atgctgacta tatggcaaca cttgctttga tcgcgacaag catcgagaaa 2760 ttcgctgtgg aaatccgcgg actgcaaaag agtgaaacac gtgaagtaga ggaatttttc 2820 gcgaaagggc aaaagggttc atctgcaatg ccgcataaac gaaatccgat tggctctgaa 2880 aacatgacag gcatggcgcg cgtgatccgc ggctacatga tgacagctta cgaaaatgtt 2940 ccattatggc atgagcgcga tatttctcat tcttcagcag aacgaattat tcttccggat 3000 gcgacaattg cgctgaacta catgctgaac cgcttctcca acatcgtgaa gaacttaacg 3060 gtcttcccgg aaaacatgaa gcgcaacatg gaccgcactc tcggccttat ctattctcag 3120 cgtgtgctcc ttgctttgat tgacacaggc ctgactcgtg aagaagccta tgatacagtt 3180 cagccgaaag caatggaagc gtgggaaaaa caagtgccgt tccgcgagct tgtggaagcg 3240 gaagagaaaa tcacgtcacg tctttctcca gaaaaaattg ctgactgctt tgattacaat 3300 taccatctga aaaatgttga tctgatcttt gaacgtttag gtttagcata gaagaagctt 3360 ttacggcttc ttctaagccg ccgcagtttg aaaattccca acattcgggt taggaggcct 3420 tccgtgaata ttgtgaagaa tgaactttta tacgaaggaa aagcaaaaaa gatctacaaa 3480 accgatgacg aaaacacgct gtatgtcgtg tataaagact ccgccactgc ctttaacggc 3540 gagaaaaaag cagaaatcag cggaaaaggg cgcttaaata atgaaatttc aagcctcatt 3600 ttcaaacacc ttcatgctaa gggcattaac aatcatttta tcgagcgcat ttcggaaacg 3660 gagcagctca ttaaaaaggt aacgattgtg ccgcttgaag tcgtggtaag aaatgttgtg 3720 gcaggaagca tgtccaaacg tctcggcatt ccagaaggca cggagcttga gcagccgatt 3780 atcgagtttt actacaagga tgacgcgctg ggtgatccgc tcatcacaga agatcatatt 3840 tggcttttga aggcggcgac tcctgagcag gtagaaacca ttaagtccat tacaacaata 3900 gtaaatgaag agcttcaaag catcttcgac gattgtcatg tcagattaat agatttcaag 3960 cttgaattcg gtttagatgc agaagggcaa gtgcttttgg cggatgaaat atctcctgac 4020 acatgccgct tgtgggataa agaaacgaac gaaaagctgg acaaagattt attcagacgc 4080 aatctgggaa gcttaaccga cgcatacgaa gagattttca atagactggg aggcattcat 4140 catgtataaa gtaaaagttt atgtcagctt aaaagaaagt gtactagatc cacaagggag 4200 cgctgtccag catgccttgc acagtatgac ttacaacgaa gttcaagatg tgcgcatcgg 4260 gaaatacatg gagcttacca ttgaaaaatc tgaccgtgat cttgacgtgc tagtgaaaga 4320 aatgtgcgaa aaacttcttg cgaacacagt gattgaagat tatagatatg aggttgagga 4380 ggtagtcgca cagtgaaatt tgcggtgatt gtgttacccg gctccaactg tgatatcgat 4440 atgtatcatg ctgtaaagga tgagctcggc catgaagtgg aatacgtctg gcatgaggaa 4500 acaagccttg acggcttcga cggcgtgtta attccgggag gattttctta cggcgattac 4560 ttaagatgcg gcgccatcgc ccgatttgcg aatattatgc cagctgtcaa acaagcagcg 4620 gctgaaggaa aacctgttct tggcgtctgt aacggattcc agattttaca ggagcttggg 4680 ctgctgccag gcgcaatgag acgcaacaaa gatctgaagt tcatttgccg tccggttgaa 4740 ttgattgttc agaacgacga aaccttattc acagcttcct acgaaaaggg agaatcgatt 4800 acaatcccgg ttgcccatgg tgaagggaat ttctactgtg atgacgagac gcttgctaca 4860 ttaaaggaaa acaatcaaat tgctttcaca tacggctcta atattaatgg aagtgtcagc 4920 gacattgccg gtgtcgtgaa tgagaaaggc aatgtattag gcatgatgcc tcaccctgag 4980 cgcgcggtcg atgaactgct tggaagcgcc gacggtctta aattgttcca gtctatcgtg 5040 aaaaattgga gggaaactca tgtcactact gcttgaacca agtaaagaac aaataaaaga 5100 agagaaactg tatcagcaaa tgggtgtcag tgatgatgag tttgcattga tagaatccat 5160 tcttggaaga ttgccgaact acacagaaat cggaattttt tctgtcatgt ggtctgagca 5220 ttgcagctat aaaaactcaa agccgattct gcgtaaattc ccgacaagcg gcgagcgtgt 5280 gctgcagggg ccgggggaag gcgccggaat cgttgatatc ggtgataacc aagcggttgt 5340 gttcaaaatt gaatcacata accacccatc agctctcgag ccttaccaag gcgctgcgac 5400 tggcgtaggc ggaattatcc gtgatgtatt ctcaatgggt gcacgcccaa tcgctgtatt 5460 gaactctctt cgatttggtg aactgacttc accccgcgtg aagtacttgt ttgaagaagt 5520 agtagcgggt atcgccggat acggcaactg tatcggcatc ccgacagtcg gcggagaagt 5580 gcagtttgac agcagctatg aaggaaatcc gctcgtcaac gcaatgtgcg tcggtttaat 5640 caaccatgaa gacatcaaaa aaggccaggc aaagggtgtc ggcaacacag taatgtacgt 5700 aggagcgaaa acagggcgtg acggcatcca cggcgctacg tttgcttctg aagaaatgtc 5760 agactcgtct gaagaaaagc gttctgctgt ccaagtcggc gatccgttta tggagaagct 5820 tttgcttgaa gcatgtctgg aagtcatcca atgcgacgcc ttagtcggca ttcaggatat 5880 gggagctgcc ggtttaacaa gctcaagtgc agaaatggca agtaaagccg gttctggcat 5940 tgaaatgaat cttgacctga ttcctcagcg cgaaacaggc atgaccgcgt atgaaatgat 6000 gctttctgaa tcacaagaac ggatgctttt ggttattgag cgcggacgtg agcaggaaat 6060 catcgatatt tttgacaagt atgatcttga agcggtttct gtcggacatg tgacagatga 6120 taaaatgctt cgcctgacac ataaaggaga ggttgtgtgc gagctgcctg ttgatgcctt 6180 ggcagaagaa gcaccggttt accataagcc ttctcaagag cctgcttact atcgcgagtt 6240 tttggaaaca gacgttccgg ctccgcaaat tgaagatgcg aatgaaatgc tgaaggccct 6300 tcttcagcag ccgacgattg cgagtaaaga gtgggtttat gatcagtatg actacatggt 6360 gcgcacgaat acagttgtcg ctcctgggtc tgatgctggt gttctcagaa tccgcggaac 6420 gaaaaaggcg ctggcgatga cgacagactg taacgcgcgt tatctctatc ttgatcctga 6480 agtcggaggg aaaattgctg tcgctgaagc agcgcgcaac atcatttgct caggcgcaga 6540 accgcttgcg gtgacagata accttaactt cggaaaccct gagaagccgg aaatcctctg 6600 gcagatcgaa aaagcggcag acggcataag cgaagcgtgc aatgttctca gcactccggt 6660 tatcggcggt aacgtatcgc tttataacga atcaaacggc acggcgatct atccgacacc 6720 agttatcggc atggtcggcc taattgaaga tacagcgcac attacaacac agcatttcaa 6780 acaagcagga gatctcgtat acgtgatcgg cgaaacaaaa ccagagtttg ccggaagcga 6840 gctgcaaaaa atgacagaag gccgtattta cggcaaagcg ccgcaaatcg atcttgatgt 6900 agagctgtct cgtcaaaaag cactgcttga cgcgattaaa aaaggcttcg ttcaatctgc 6960 gcatgatgtg tctgaaggcg gcttaggcgt agcgattgcg gaaagtgtca tgacgacgga 7020 aaaccttggc gctaatgtga ctgtagaagg ggaagcggcg ttattattct ctgaatctca 7080 atctcgcttc gtcgtttcag tgaaaaaaga acatcaagct gcgtttgaag caactgtcaa 7140 agatgcagtt catattggtg aggtaacagc tgacggaatt ctggcgattc aaaaccaaga 7200 cggacaacaa atgattcatg cgcaaacgaa agagcttgaa cgcgtatgga aaggagctat 7260 cccatgcttg ctgaaatcaa aggcttaaat gaagaatgcg gcgtttttgg gatttgggga 7320 catgaagaag ccccgcaaat cacgtattac ggtctccaca gccttcagca ccgaggacag 7380 gagggtgctg gcatcgtagc gactgacggt gaaaagctga cggctcacaa aggccaaggt 7440 ctgatcactg aagtatttca aaacggcgaa ctcagcaaag taaagggaaa aggcgctatc 7500 gggcacgttc ggtacgcaac ggctggaggc ggcggatacg aaaatgttca gccgctcctc 7560 ttccgttccc aaaacaacgg cagcctggcg cttgctcata acggaaatct tgtcaacgcc 7620 actcagctga agcagcagct cgaaaatcaa gggagcatct ttcaaacctc ttcggataca 7680 gaggttttgg ctcacctgat caaaagaagc ggacacttca cgctgaagga tcaaattaaa 7740 aactcgcttt ctatgctgaa aggcgcctac gcgttcctga tcatgaccga aacagaaatg 7800 attgtcgcac ttgatccaaa cgggctgaga ccgctatcca tcggcatgat gggcgacgct 7860 tatgtggtcg catcagaaac atgcgcattt gacgtcgtcg gcgcaacgta ccttcgcgag 7920 gtagagccgg gagaaatgct gatcattaat gatgaaggca tgaaatcaga gcgtttttcc 7980 atgaatatca atcgttccat ttgcagcatg gagtacattt atttctccag accagacagc 8040 aatattgacg gtattaatgt gcacagtgcc cgtaaaaacc ttgggaaaat gctggctcag 8100 gaatccgcag ttgaagctga cgtcgtaacc ggggttccgg attccagtat ttcagcggcg 8160 atcggctatg cagaggcaac aggcattccg tatgagcttg gcttaatcaa aaaccgttat 8220 gttggcagaa cgtttattca gccgtcccag gctctgcgtg agcaaggcgt cagaatgaag 8280 ctgtctgcgg tgcgcggggt tgtagaaggc aaacgcgtcg tgatggtgga tgactctatc 8340 gtgcgaggaa caactagccg ccggattgtc acgatgctaa gagaggcggg tgcgacagag 8400 gtgcatgtga aaatcagttc accgccgatc gctcatccgt gcttttacgg cattgacact 8460 tccacacatg aagaactgat cgcgtcttcg cattctgtcg gagaaatccg tcaggaaatc 8520 ggagccgata ccctctcatt tttgagtgtg gaagggctgc tgaaaggcat cggcagaaaa 8580 tacgatgact cgaattgcgg acagtgtctc gcttgcttta caggaaaata tccgactgaa 8640 atttaccagg atacagtgct tcctcacgta aaagaagcag tattaaccaa ataaaacttg 8700 aaaaatgaca taaaggcagc gcagttcggc tgcctttctc tttctgccct cgttcgggga 8760 gatattttga aaagcgcctt aaaggagtga ataggatgtc tgaagcatat aaaaacgcag 8820 gagttgacat cgaagccgga tatgaagctg taaaacgaat gaaaaaacac gtggagcgca 8880 cgaaacggct tggcgttatg ggcagccttg gcggttttgg cggcatgttt gacctgtctg 8940 agctttctta tcaaaaacca gttctcattt cgggaacgga cggtgtcggc acaaaattaa 9000 agctcgcttt ttccatggat aagcatgaca cgattggcgt ggatgctgtt gcaatgtgtg 9060 tcaatgacgt gctggcacaa ggtgcagagc cgctgttttt cctcgattat ttagcggttg 9120 gcaaagcgga cccagtgaaa attgaacaga ttgtacaagg tgttgcggaa ggctgtgagc 9180 agtcaggttc agccttagtc ggcggtgaaa cggctgaaat gccggggctc tatacagctg 9240 atgaatacga tattgccggt ttctcagtcg gagtggcaga aaaggacgaa atcgtgactg 9300 gagaaaaaat tgaagagggc catctgttga tcggcctcag ctccagcggc cttcacagca 9360 acggcttttc ccttgtcaga aaagtgcttc tggatgatgc ggcgtgggac ctcgatacaa 9420 catatgagcc gtttgaacgc ccgcttgggc aggaattggt tgaaccgaca aggatttacg 9480 tgaagcctgt gcttgccgcg gtcaaaagcg gaaaaatcga cggcatggcg cacgtgacag 9540 gcggaggatt tattgaaaat attccgcgta tgcttccgga aggcttaagt gcggaaattg 9600 atcatggctc atggccgatc ccgccgattt tttctttctt gcaagagtac ggcaagctaa 9660 aggaagaaga catgttcaac gtctttaata tgggtatcgg ttttgttttg gcagtcaaag 9720 aagagcatct gacagatgtg atcggaacgc ttgaaagcca tggcgaaaaa gcctatttaa 9780 tcgggcgtgt gaaaaaaggt gaaggcgtca cattcggcgg tgcggcactt tcatgaaaaa 9840 gtttgcggta tttgcatcag gaaacggttc aaacttcgaa gccatcgtca cgcgtttgaa 9900 ggaggagaac tgggatgcgt cacgagcgct cctcgtttgc gacaaaccgc aggcgaaagt 9960 catcgaacgg gcggaacgat tccacattcc atccttcgca tttgagccga agtcttatga 10020 aaacaaggct gcatttgaac aagccatcat tgaacagctt cgtcttcacg aggttgaatt 10080 gattgctctt gccggctata tgaggctgat cggtgatacg ctccttcaag catatggggg 10140 aaaaatcatt aacattcacc catcgcttct tccggcgttt cctggaatcg acgcagtcgg 10200 acaggcgttc cgggcgggtg tgaaggtggc cggaatcacc gtgcattatg tcgatgaagg 10260 aatggataca ggtccgatca tcgctcaaaa ggcaatcgaa attgatgaac atgatacatt 10320 ggaaacaatc gaacagcgaa ttcacaagct tgagcataaa tggtatccga gtgtgattaa 10380 acagctatta ggattaaata acagaggtga aaaggcatga ccattaaacg tgcattaatc 10440 agtgtttcag ataaaacaaa tctcgttcct ttcgtaaaag aactgacaga gcttggcgtt 10500 gaagtcatct ccacaggcgg aacgaaaaag cttcttcaag aaaacggtgt ggatgtgatc 10560 ggaatttctg aagtgacagg ctttcctgaa attatggacg gccggttaaa aaccctacac 10620 ccgaatattc acggcggcct tctggcggtt cgcggcaatg aagagcatat ggcgcagatc 10680 aatgaacacg ggattcagcc gattgacctc gttgtcgtca acctctatcc atttaaagaa 10740 acgatttcta aagaagacgt cacgtatgaa gaagcgatcg aaaatatcga catcggcgga 10800 cctggcatgc tgcgcgcggc atcaaagaac catcaggatg tcacggttat cgtcgatccg 10860 gccgattaca gcccagtgct aaatcaaatc aaagaagaag gcagcgtatc tcttcagaaa 10920 aaacgcgagc tcgcggcaaa agtattccgt catactgcgg catatgatgc actgatcgct 10980 gactatctga caaatgttgt cggtgaaaaa gaaccagagc aattcactgt gacatttgag 11040 aaaaaacaat cgcttcgcta tggagaaaac ccgcatcagg aagcaacttt ctatcaaaca 11100 gctcttcctg tcaaaggctc cattgcgcaa gcagaacagc ttcacggaaa agagctttct 11160 tacaacaaca ttaaagacgc ggatgctgca gttcaaatcg ttcgtgaatt cactgaaccg 11220 gctgctgttg ctgtgaagca tatgaacccg tgcggcgtgg gaacaggaaa aacgatcgca 11280 gaagcgtttg acagagcgtt tgaagcggat aaaacatcta tcttcggcgg cattatcgcg 11340 ctgaaccgtg aagtggacaa ggcaactgcc gaagcgcttc acaacatttt cttagaaatc 11400 atcattgcgc cttcattcag ccaagaagcg ctcgacgtcc tgactgcgaa gaaaaatctc 11460 cgtctcgtga cgcttgacgt atccgccgct gttcaaaagg aaaaacagct gacatccgtt 11520 caaggcgggc tgctgattca agatttagat atgcacggct tcgatgatgc tgagattagc 11580 attccgacaa aaagagagcc gaacgagcaa gagtgggaag acttgaagct tgcttggaaa 11640 gtcgtgaagc atgtgaaatc aaatgcgatt gttctcgcga aggacaacat gacagtcggc 11700 gtgggagcag gccaaatgaa ccgcgtcgga tcggcaaaaa tcgcaatcga gcaagcaggg 11760 gaaaaagcga agggcagcgc gctcggttcg gatgcatatt tcccaatgcc agatactgtc 11820 gaagaagcgg caaaagcggg cgttacagcc atcattcagc caggcggatc gatccgagat 11880 gaggattcca tcaaaaaagc ggatgaatac ggcattgcca tggtattcac cggcatcaga 11940 cacttcaaac attaagggga tgaaaacgac gtgaatgtat taattatcgg taaaggcgga 12000 agagaacata cgctggcgtg gaaggcagcg caaagcagcc tcgtcgagaa tgtatttgcc 12060 gctcccggaa atgacggcat ggcagcttcc gctcagcttg taaacattga ggaaagcgac 12120 cacgcagggc ttgtctcatt tgcaaaacaa aatcaggtcg gcctgaccat tgtcggccct 12180 gaggttcctt taattgaagg tctggtggat gaattcgaaa aagcgggctt acatgtgttc 12240 ggtccgtcaa aagctgcggc gatcatcgaa ggaagcaaac agttcgctaa ggatttaatg 12300 aagaaatacg acattccgac cgcagaatac gagacgttta catcctttga tgaggcgaag 12360 gcatatgtgc aggaaaaagg tgctccgatt gtgataaaag cagatggact tgcagctgga 12420 aaaggcgtta ctgtggctat gacagaggaa gaagcaattg cgtgcttgca tgactttctt 12480 gaagatgaaa agttcggtga tgcgagcgcg tccgttgtca ttgaagaata tctttctggt 12540 gaagaatttt ctctaatggc ctttgtcaaa ggggaaaagg tgtatccgat ggtgattgcc 12600 caggatcaca agcgggcgtt tgacggagac aaaggcccga atacaggcgg catgggcgcc 12660 tactcgccag ttccgcaaat ttcggaagaa acggtccgcc atgctgtaga aacgatcgtt 12720 aagccggctg caaaagcaat ggtacaagaa ggccgttcct tcactggcgt tttgtacgct 12780 ggattgatgc ttactgaaaa cggctcgaag gtcattgaat ttaatgcccg tttcggcgat 12840 ccggaaacac aggtcgtgct tccgcgcatg gaatctgatc tggtacaggt gcttcttgat 12900 cttttagatg ataaggaagt tgacttaaga tggaaggata ccgcggcagt gagtgttgtg 12960 cttgcatcag aagggtatcc ggaaagctat gcaaaaggca cgccgatcgg cagccttgca 13020 gcagaaactg agcaggtcgt ggtcttccat gccggaacga aagcagaagg tggagagttc 13080 gttacaaacg gggggcgcgt cgccaatgtg acggcttttg atgaaacgtt tgaagcggct 13140 agagaccgag tgtacaaagc ggttgatgaa attttcaaac cgggactctt tttcagaaaa 13200 gacattgggg cacgcgcttt aaaggctgcc caaaaataag tgaggaaaac ccgcagaata 13260 gctgcgggtt ttttgttatc aaaaagcggg atggcagcta gaagaagaaa gcacaacgag 13320 cgtggttggt tttccatgg 13339 // |
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