 |
|
EBI DbfetchID J02591; SV 1; linear; genomic DNA; STD; UNC; 4347 BP. XX AC J02591; XX DT 19-SEP-1987 (Rel. 13, Created) DT 15-JAN-2007 (Rel. 90, Last updated, Version 7) XX DE Plasmid R773 arsenical resistance operon genes, arsA, arsB and arsC, DE complete cds. XX KW arsenical pump; arsenical resistance; ATPase; membrane protein. XX OS Plasmid R773 OC other sequences; plasmids. XX RN [1] RP 1-4347 RX PUBMED; 3021763. RA Chen C.M., Misra T.K., Silver S., Rosen B.P.; RT "Nucleotide sequence of the structural genes for an anion pump. The RT plasmid-encoded arsenical resistance operon"; RL J. Biol. Chem. 261(32):15030-15038(1986). XX RN [2] RP 1-4347 RA Rosen B.P.; RT ; RL Submitted (10-SEP-1986) to the EMBL/GenBank/DDBJ databases. RL Barry P. Rosen, Department of Biochemistry, Wayne State University School RL of Medicine, Detroit, MI 48201, USA XX FH Key Location/Qualifiers FH FT source 1..4347 FT /organism="Plasmid R773" FT /mol_type="genomic DNA" FT /clone="pUM3" FT /db_xref="taxon:2497" FT RBS 174..179 FT /gene="arsA" FT /note="putative" FT CDS 184..1935 FT /codon_start=1 FT /transl_table=11 FT /gene="arsA" FT /product="ArsA" FT /note="pump-driving ATPase" FT /db_xref="GOA:P08690" FT /db_xref="InterPro:IPR003348" FT /db_xref="InterPro:IPR003593" FT /db_xref="InterPro:IPR016300" FT /db_xref="PDB:1F48" FT /db_xref="PDB:1IHU" FT /db_xref="PDB:1II0" FT /db_xref="PDB:1II9" FT /db_xref="UniProtKB/Swiss-Prot:P08690" FT /protein_id="AAA21094.1" FT /translation="MQFLQNIPPYLFFTGKGGVGKTSISCATAIRLAEQGKRVLLVSTD FT PASNVGQVFSQTIGITIQAIASVPGLSALEIDPQAAAQQYRARIVDPIKGVLPDDVVSS FT INEQLSGACTTEIAAFDEFTGLLTDASLLTRFDHIIFDTAPTGHTIRLLQLPGAWSSFI FT DSNPEGASCLGPMAGLEKQREQYAYAVEALSDPKRTRLVLVARLQKSTLQEVARTHLEL FT AAIGLKNQYLVINGVLPKTEAANDTLAAAIWEREQEALANLPADLAGLPTDTLFLQPVN FT MVGVSALSRLLSTQPVASPSSDEYLQQRPDIPSLSALVDDIARNEHGLIMLMGKGGVGK FT TTMAAAIAVRLADMGFDVHLTTSDPAAHLSMTLNGSLNNLQVSRIDPHEETERYRQHVL FT ETKGKELDEAGKRLLEEDLRSPCTEEIAVFQAFSRVIREAGKRFVVMDTAPTGHTLLLL FT DATGAYHREIAKKMGEKGHFTTPMMLLQDPERTKVLLVTLPETTPVLEAANLQADLERA FT GIHPWGWIINNSLSIADTRSPLLRMRAQQELPQIESVKRQHASRVALVPVLASEPTGID FT KLKQLAG" FT RBS 1974..1979 FT /gene="arsB" FT /note="putative" FT CDS 1984..3273 FT /codon_start=1 FT /transl_table=11 FT /gene="arsB" FT /product="ArsB" FT /note="arsenical pump protein" FT /db_xref="GOA:P08691" FT /db_xref="InterPro:IPR000802" FT /db_xref="UniProtKB/Swiss-Prot:P08691" FT /protein_id="AAA21095.1" FT /translation="MLLAGAIFILTIVLVIWQPKGLGIGWSATLGAVLALASGVIHIAD FT IPVVWNIVWNATATFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTYIVLLGAA FT VAALFANDGAALILTPIVIAMLLALGFSKSTTLAFVMAAGFISDTASLPLIVSNLVNIV FT SADFFKLGFTEYASVMVPVDIAAIIATLVMLHLFFRKDIPPTYELARLKEPAKAIKDPA FT TFRTGWVVLLLLLVGFFVLEPMGIPVSAIAAVGAAVLFAVAKKGHGINTGKVLRGAPWQ FT IVIFSLGMYLVIYGLRNAGLTDYLSDVLNELADKGLWAATLGTGFLTALLSSIMNNMPT FT VLIGALSIDGSTATGVIKEAMIYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTITW FT GYYFRTGIVMTLPVLFVTLAALALRLSVTL" FT RBS 3275..3278 FT /gene="arsC" FT /note="putative" FT CDS 3286..3711 FT /codon_start=1 FT /transl_table=11 FT /gene="arsC" FT /product="ArsC" FT /note="arsenical pump modifier" FT /db_xref="GOA:P08692" FT /db_xref="InterPro:IPR006659" FT /db_xref="InterPro:IPR006660" FT /db_xref="InterPro:IPR012335" FT /db_xref="InterPro:IPR012336" FT /db_xref="PDB:1I9D" FT /db_xref="PDB:1J9B" FT /db_xref="PDB:1JZW" FT /db_xref="PDB:1S3C" FT /db_xref="PDB:1S3D" FT /db_xref="PDB:1SD8" FT /db_xref="PDB:1SD9" FT /db_xref="PDB:1SJZ" FT /db_xref="PDB:1SK0" FT /db_xref="PDB:1SK1" FT /db_xref="PDB:1SK2" FT /db_xref="UniProtKB/Swiss-Prot:P08692" FT /protein_id="AAA21096.1" FT /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIILYLENPPSRDELV FT KLIADMGISVRALLRKNVEPYEQLGLAEDKFTDDQLIDFMLQHPILINRPIVVTPLGTR FT LCRPSEVVLDILQDAQKGAFTKEDGEKVVDEAGKRLK" XX SQ Sequence 4347 BP; 979 A; 1082 C; 1165 G; 1121 T; 0 other; agcttccggg gcagaaggtc tgccattgtt gttactggat ggcgaaacag tgatggccgg 60 gcgttacccg aaacgcgctg agctggctcg ctggtttggc attccactgg ataaagtggg 120 attagcgcct tcaggttgct gtggtggtaa tacttcttgt tgttaagtat atcaggagga 180 catatgcaat tcttacagaa tatcccccct tatctgtttt ttacgggtaa aggaggcgtg 240 ggtaaaacct ctatttcctg cgccacggcg atccgtctgg cagaacaggg gaaacgggtg 300 ctgctggtca gtaccgatcc ggcctcaaac gtaggccagg tgtttagcca gacgattggc 360 attaccattc aggcaatagc ctctgttcct ggattgtcgg ctcttgagat tgatcctcag 420 gctgctgcac aacagtaccg ggccagaatc gttgacccta ttaaaggcgt cctgcctgat 480 gacgttgttt ccagcatcaa cgaacaactg tcaggtgcat gcacaacaga gattgcggct 540 tttgatgaat ttaccggatt actgacagat gcctctctgc tgacgcggtt tgaccatatc 600 atttttgata ccgcgccgac gggtcacacc attcgccttc tgcagttgcc gggcgcctgg 660 agtagcttta ttgacagcaa tcccgagggc gcgtcctgtc tcggcccaat ggcggggctg 720 gaaaagcagc gtgaacagta tgcctatgct gttgaagcgt tgtctgatcc aaagcgtacc 780 cgactggtat tagttgctcg tctgcaaaaa tccacgttgc aggaagtcgc ccggactcat 840 ctggaacttg ccgccatcgg ccttaaaaat cagtacctgg ttattaatgg cgttctgccc 900 aaaactgaag cggcaaacga tacactggct gctgcaatat gggagcgtga acaggaggcg 960 ctggccaatc ttcccgctga tcttgcaggt ttgccaactg acacattatt tctccagccg 1020 gtcaatatgg tcggtgtgtc tgcgctcagc aggcttctct ccactcagcc tgtagcgtcg 1080 ccatcctcgg atgaatacct gcaacagcgg cctgatattc cgtcactttc tgcgttggtt 1140 gatgatattg cccgtaatga acatggcctg attatgctga tgggtaaagg tggcgtgggg 1200 aaaaccacga tggctgctgc cattgctgtc aggctggccg acatgggatt tgatgtccat 1260 ctgacaacat ctgatcctgc ggcgcatctc agcatgaccc tgaacggtag ccttaacaac 1320 ctgcaggtca gcaggatcga tcctcacgag gaaacggaac gctatcgtca gcatgttctt 1380 gaaacaaagg gaaaagaact ggacgaagcg ggaaaacgcc tgctggaaga ggacttacgc 1440 tcaccttgca ctgaggaaat tgctgttttc caggcctttt cacgggtgat tcgtgaagcg 1500 ggtaaacgtt tcgtggtgat ggatacggct ccgaccggac acacgctgtt gctgctggat 1560 gctacaggcg cgtaccaccg cgaaattgcg aagaaaatgg gagaaaaagg ccatttcacc 1620 acaccgatga tgctgcttca ggatccggag cgcactaaag tgttactggt cacgctgccg 1680 gaaaccacgc ccgtgcttga agcggcaaat ttacaggccg accttgagcg tgcaggcatt 1740 cacccctggg gctggattat caataacagc ctttccattg cggatacccg ttcgccactg 1800 cttcgtatgc gggcacaaca ggaacttccg cagatagaga gcgttaaacg ccagcacgcc 1860 agccgtgtcg ctcttgttcc cgtactggcg tcagaaccaa ccggcatcga caaactcaaa 1920 caacttgctg ggtaaattat tgtcatcacg tagggcagca aagctgccct gtcaggaggt 1980 tttatgttac tggcaggagc catttttatc ctgaccatcg ttttggttat ctggcagccg 2040 aaaggattag ggattggctg gagcgccacg ttgggggctg tactggctct ggcgtcgggt 2100 gtgatacaca ttgctgatat tccggtggtg tggaatattg tctggaacgc gacggcaaca 2160 tttatcgccg ttattatcat cagcctgttg ctggatgagt caggcttttt cgaatgggca 2220 gcgttgcacg tttctcgctg ggggaacggt cgtggccgcc tgctgtttac gtatattgtt 2280 ctgctcggcg cggcagtggc ggcactgttt gccaacgatg gtgcggctct tattctgacg 2340 ccgattgtta tcgccatgct gctggcgctt ggcttcagta aaagcaccac actggcattc 2400 gtgatggctg ccgggtttat ttccgatact gctagcttgc cgcttatcgt atcgaacttg 2460 gtcaatatcg tttcagctga tttcttcaaa ctgggattca cggagtatgc ttcggtgatg 2520 gtgccggtgg atatcgccgc cattatcgcc acgctggtga tgctgcatct ttttttccgc 2580 aaagatatcc cccccactta tgagctggcg cgactgaaag aacctgcaaa agccatcaaa 2640 gatcctgcga cgttcagaac cggctgggtt gttctcctcc ttctgctggt cggttttttt 2700 gtccttgagc cgatggggat ccctgtgagt gcgattgcag cagtgggggc ggcagtcctg 2760 tttgcggtgg ccaaaaaagg tcatggtatt aacaccggta aagtgctgcg cggcgcgccc 2820 tggcagatcg tgatcttctc tctggggatg tatctggtga tctacgggtt gcgtaacgcc 2880 ggtctgacag attatctctc tgacgtgctg aacgaactgg cagataaagg actgtgggcc 2940 gccacgctgg ggacgggatt cctgacagca ttactctctt ctattatgaa caatatgccg 3000 acagtattga ttggcgcatt gtcaattgat ggcagcaccg cgaccggtgt tatcaaagag 3060 gcgatgattt atgccaacgt cattggctgc gatctggggc cgaaaatcac ccctattggt 3120 agcctggcaa cactgctctg gctgcatgta ctttcacaga agaacatgac catcacttgg 3180 ggatactatt tccgcaccgg gatcgtcatg actctgcctg tgctgtttgt aacgctggcc 3240 gcgctggcgc tacgtctctc tgtcacattg taatgagatt ctgatatgag caacatcact 3300 atttatcata acccagcctg cggcacctcg cgtaatacgc tggagatgat ccgcaacagc 3360 ggtaccgagc cgaccattat tctttacctt gaaaacccgc cttcgaggga tgagctggtt 3420 aaacttattg ccgatatggg tatttcagta cgagcgctgc tgcgtaaaaa cgttgaacct 3480 tacgagcaac tgggtcttgc agaagataaa tttactgacg atcagctcat cgactttatg 3540 ttgcaacacc caattctgat taaccgtccg atcgtggtta cgccgctggg aaccagactg 3600 tgccgtcctt ctgaagtggt tctggatatc ctacaggatg cgcagaaagg ggctttcact 3660 aaggaagacg gtgaaaaagt cgttgatgaa gcaggaaaac ggctgaaata aataataagg 3720 gggcgaatgc ccccttattc gtacctcaga gcatcatgcc tgaatccgat ggtaacgtgc 3780 tgttcccttc gcccagattt cgttgcagca acactgtgtc aagccaacga ccatgcttga 3840 agccaatatt tttcagcgta ccaatctcag taaatccaac ctgctgatgg actttcagcg 3900 aaccttgatt attgctatcg ccgacaatag caatcatctg ccgatagccg tgagtttttg 3960 cccaggaaat gacgtagcgc agcaacgctt ttcctgtccc ccgacgctgt gcgtccgggt 4020 gaatataaat cgaatcttcg agagtgtggc ggtaggcgta gcgttcacgg taacgagtga 4080 gataacaata cccgataacc ttttcttcct ctaacgtgac aacccacggt agctcctgat 4140 tgcggatctt tttcaggcgg gcaagcatct cgtgggtatc cggaggttca gtttcaaagc 4200 tggcggtacc gtgaagaaca tgatgggcgt agatatcgcg tatagcgggg atgtgctttt 4260 cttctgcgtt aacgattttc attgttctgt tcccgttggc atctttttca aggttacttg 4320 aaaatccaga ggaaaggtaa atacaaa 4347 // |
|