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EBI DbfetchID J02584; SV 1; linear; genomic DNA; STD; PRO; 5482 BP. XX AC J02584; M24505; XX DT 20-NOV-1990 (Rel. 25, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 11) XX DE B.subtilis (gluconate operon) gntR, gntK and gntP genes encoding gnt DE repressor, gluconate kinase and permease, and gntZ gene. XX KW gluconate kinase; gluconate operon; gnt repressor; gntZ gene product; KW permease. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-742 RX DOI; 10.1093/nar/14.3.1237 RX PUBMED; 2419835. RA Fujita Y., Fujita T.; RT "Identification and nucleotide sequence of the promoter region of the RT Bacillus subtilis gluconate operon"; RL Nucleic Acids Res. 14(3):1237-1252(1986). XX RN [2] RP 1-5482 RX PUBMED; 3020045. RA Fujita Y., Fujita T., Miwa Y., Nihashi J., Aratani Y.; RT "Organization and transcription of the gluconate operon, gnt, of Bacillus RT subtilis"; RL J. Biol. Chem. 261(29):13744-13753(1986). XX RN [3] RX PUBMED; 2492998. RA Fujita Y., Miwa Y.; RT "Identification of an operator sequence for the Bacillus subtilis gnt RT operon"; RL J. Biol. Chem. 264(7):4201-4206(1989). XX DR GOA; P42100. DR InterPro; IPR004381; Glycerate_kinase. DR InterPro; IPR018193; Glyc_kinase_flavodox-like-dom. DR UniProtKB/Swiss-Prot; P42100; GLXK_BACSU. XX CC On Feb 8, 2002 this sequence version replaced gi:341178. CC Draft entry and clean copy sequence for [2] kindly provided by CC Y.Fujita, 10-SEP-1986. CC The gnt ribosome binding sites are located at positions 218-231 CC (gntR), 940-951 (gntK), 2514-2521 (gntP) and 3883-3889 (gntZ). CC Three potential stem-loop structures are found at positions 116-132 CC [2], 192-209 [2], and 5314-5348 (potential transcription CC terminator) [1]. XX FH Key Location/Qualifiers FH FT source 1..5482 FT /organism="Bacillus subtilis" FT /strain="Marburg 168" FT /mol_type="genomic DNA" FT /clone="phi-105gnt" FT /db_xref="taxon:1423" FT -35_signal 165..170 FT /gene="gnt" FT /citation=[3] FT -10_signal 188..193 FT /gene="gnt" FT /citation=[3] FT mRNA 202..5344 FT /note="gnt major mRNA (alt.)" FT CDS 236..967 FT /codon_start=1 FT /transl_table=11 FT /product="gnt repressor" FT /db_xref="GOA:P10585" FT /db_xref="InterPro:IPR000524" FT /db_xref="InterPro:IPR011711" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/Swiss-Prot:P10585" FT /protein_id="AAA56924.1" FT /translation="MLDSKDLLYPAKWLSKASTGVRVAYELRMRIVSGLIESGTILSEN FT TIAAEFSVSRSPVREALKILASEKIIRLERMGAVVIGLTEKKIAEIYDVRLLLETFVFE FT RLVKIDIEPLVKDLSKILEMMKVSIKYEDADEFSFQDVLFHETIIRAIDHSYIQMIWNN FT LKPVMESFILLSMRVRLKEKYEDFTRILDNHELYIQAIKTKDRALMIQSLHQNFDDVQD FT KVEDLWLSQQMLAKGAEYNND" FT CDS 960..2501 FT /codon_start=1 FT /transl_table=11 FT /product="gluconate kinase" FT /db_xref="GOA:P12011" FT /db_xref="InterPro:IPR000577" FT /db_xref="InterPro:IPR006002" FT /db_xref="InterPro:IPR018483" FT /db_xref="InterPro:IPR018484" FT /db_xref="InterPro:IPR018485" FT /db_xref="UniProtKB/Swiss-Prot:P12011" FT /protein_id="AAA56925.1" FT /translation="MTSYMLGIDIGTTSTKAVLFSENGDVVQKESIGYPLYTPDISTAE FT QNPEEIFQAVIHTTARITKQHPEKRISFISFSSAMHSVIAIDENDKPLTPCITWADNRS FT EGWAHKIKEELNGHEVYKRTGTPIHPMAPLSKIAWITNERKEIASKAKKYIGIKEYIFK FT QLFNEYVIDYSLASATGMMNLKGLDWDEEALRIAGITPDHLSKLVPTTEIFQHCSPEIA FT IQMGIDPETPFVIGASDGVLSNLGVNAIKKGEIAVTIGTSGAIRTIIDKPQTDEKGRIF FT CYALTDKHWVIGGPVNNGGIVLRWIRDEFASSEIETATRLGIDPYDVLTKIAQRVRPGS FT DGLLFHPYLAGERAPLWNPDVRGSFFGLTMSHKKEHMIRAALEGVIYNLYTVFLALTEC FT MDGPVTRIQATGGFARSEVWRQMMSDIFESEVVVPESYESSCLGACILGLYATGKIDSF FT DAVSDMIGSTYRHTPIEDSAKEYRTLMPIFINLSRLLENQYTQIADYQRGLITHK" FT CDS 2530..3876 FT /codon_start=1 FT /transl_table=11 FT /product="permease" FT /db_xref="GOA:P12012" FT /db_xref="InterPro:IPR003474" FT /db_xref="UniProtKB/Swiss-Prot:P12012" FT /protein_id="AAA56926.1" FT /translation="MPLIIVALGILALLFLIMGLKLNTFISLLVVSFGVALALGMPFDK FT VVSSIEAGIGGTLGHIALIFGLGAMLGKLIADSGGAQRIAMTLVNKFGEKNIQWAVVIA FT SFIIGIALFFEVGLVLLIPIVFAISRELKISILFLGIPMVAALSVTHGFLPPHPGPTAI FT AGEYGANIGEVLLYGFIVAVPTVLIAGPLFTKFAKKIVPASFAKNGNIASLGTQKTFNL FT EETPGFGISVFTAMLPIIIMSVATIIDLLQETIGFADNGVLAFIRLIGNASTAMIISLL FT VAVYTMGIKRNIPVKTVMDSCSTAISQIGMMLLIIGGGGAFKQVLINGGVGDYVADLFK FT GTALSPIILAWLIAAILRISLGSATVAALSTTGLVIPLLGHSDVNLALVVLATGAGSVI FT ASHVNDAGFWMFKEYFGLSMKETFATWTLLETIISVAGLGFILLLSLVV" FT mRNA 3873..5344 FT /note="gnt minor mRNA (alt.)" FT CDS 3899..5305 FT /codon_start=1 FT /transl_table=11 FT /gene="gntZ" FT /note="putative" FT /db_xref="GOA:P12013" FT /db_xref="InterPro:IPR006113" FT /db_xref="InterPro:IPR006114" FT /db_xref="InterPro:IPR006115" FT /db_xref="InterPro:IPR006183" FT /db_xref="InterPro:IPR006184" FT /db_xref="InterPro:IPR008927" FT /db_xref="InterPro:IPR012284" FT /db_xref="InterPro:IPR013328" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P12013" FT /protein_id="AAA56927.1" FT /translation="MFNSIGVIGLGVMGSNIALNMANKGENVAVYNYTRDLTDQLIQKL FT DGQSLSPYYELEDFVQSLEKPRKIFLMVTAGKPVDSVIQSLKPLLEEGDVIMDGGNSHY FT EDTERRYDELKEKGIGYLGVGISGGEVGALTGPSIMPGGDRDVYEKAAPILTKIAAQVG FT DDPCCVYIGPKGAGHFTKMVHNGIEYADMQLIAEAYTFLRETLRLPLDEIASIFETWNQ FT GELKSYLIEITAEILRKKDEKTGQPLIDVILDKTGQKGTGKWTSMQAIDNGIPSTIITE FT SLFARYLSSLKEERMAAQDVLAGPEAEEKHLDKDTWIEYVRQALYMGKVCAYAQGFAQY FT KMSSELYGWNLPLKDIALIFRGGCIIRADFLNVISEAFSEQPNLANLLIAPYFTDKLHA FT YQTGLRKVVCEGISTGISFPCLTTALSYYDGYRTGRSNANLLQAQRDYFGAHTYERTDM FT DGVFHTNWSE" XX SQ Sequence 5482 BP; 1603 A; 1227 C; 1279 G; 1373 T; 0 other; agctttcctt aaaagaatca ggtgcaatga tgattttcat ccaaatcgcc tctttcaaaa 60 catgattcac ttaacctatt gatctccaat gtaccataat tgatctggaa atacatacca 120 tgcaatatgg taaaaattta aataaaaatt agaaatgaaa gtgtttgcat aaaagaaata 180 ttcacgttat catacttgta tacaagtata ctccttgagt gaggaaggtg agtgtatgct 240 agactccaaa gacctgttgt atcccgcaaa atggctctca aaagcgtcaa ccggagttcg 300 tgtcgcatac gagctgagaa tgcggatcgt ttcaggtctg attgaaagcg gtaccatttt 360 atcagaaaat acaatcgccg ccgagttttc agtaagccgt tcgccggttc gcgaagcgct 420 aaaaatactc gcatccgaaa aaatcatccg cttagaacga atgggagcgg tcgtaattgg 480 tttaactgag aagaaaatcg cggaaattta tgatgtgcgg ttactattag aaacatttgt 540 ctttgaacgg cttgtcaaaa tagacattga gcctttagtt aaggatctca gcaaaattct 600 tgaaatgatg aaagtctcaa tcaaatatga ggatgctgac gaattttcat ttcaagatgt 660 gctgttccat gaaacgatta tccgagcgat tgatcattca tacattcaga tgatctggaa 720 caatctaaaa cccgtcatgg aaagctttat tcttttatcg atgcgggtac ggttaaagga 780 aaagtatgaa gacttcacaa ggattttaga taaccacgag ctttatattc aagccatcaa 840 aacaaaggat agggcgctga tgattcagtc tcttcaccaa aactttgatg atgtgcagga 900 taaggtagaa gacctatggc tctcacaaca aatgctggca aaaggagctg aatacaacaa 960 tgactagtta tatgttagga atcgatatcg gcacgacaag cacaaaagct gtgctgttca 1020 gtgaaaacgg agatgtggta cagaaagaaa gcatcggcta cccgctctat acaccggaca 1080 tctcaacagc tgaacaaaat ccggaagaga tttttcaggc agtcatccat acaacagcga 1140 gaattacaaa acagcatccc gaaaaacgga tctctttcat ttcgttcagc agcgccatgc 1200 acagcgtcat tgcaatcgac gaaaatgaca agccgctgac gccatgcatt acatgggccg 1260 acaacagaag tgaaggctgg gcgcataaga tcaaagagga attgaacgga catgaggtgt 1320 acaaacggac gggaacaccg attcatccga tggcaccgtt aagtaaaatt gcctggatca 1380 cgaatgaaag aaaagaaatc gcttccaagg caaaaaaata tatcggcatc aaagaataca 1440 tttttaaaca gctgttcaat gagtatgtca tcgattactc attggcatca gcgacaggca 1500 tgatgaacct gaagggcttg gattgggacg aagaggcact gcgcatcgca ggcatcacgc 1560 cggatcactt atccaaactc gtgccgacaa ccgagatctt tcagcactgc agcccggaaa 1620 tagcgatcca aatgggaatc gatccggaaa cgccttttgt catcggcgcc agcgacggcg 1680 tgctgtccaa cctcggcgtc aacgccatta aaaagggtga aatcgccgtc accatcggta 1740 caagcggtgc cattcggacg attattgaca agccgcaaac cgatgaaaaa gggagaattt 1800 tttgctatgc cttgacggac aagcattggg ttattggggg acctgtgaac aatgggggca 1860 tcgtccttcg ctggatcaga gacgagtttg cctcctccga aatcgaaaca gcgacacggc 1920 tgggcattga tccatatgac gtgctgacga aaattgccca acgcgtcaga cccggttctg 1980 acggtctgct gttccaccca tatctcgccg gagaacgcgc tccgttgtgg aatccggatg 2040 tacgcggctc atttttcggc ttgaccatgt cgcataagaa agagcatatg atacgcgcgg 2100 cattggaagg cgtcatttac aacctgtaca cggtgttctt ggcgttaact gaatgcatgg 2160 atggcccggt aacccgcatc caagcgacag ggggttttgc aaggtcggag gtttggcgcc 2220 aaatgatgtc ggatatcttc gaatcagagg ttgtcgttcc ggaaagctac gaaagctcat 2280 gtctcggcgc ctgcatttta ggcctgtatg caacaggaaa aatcgattct ttcgacgccg 2340 tgtccgatat gatcggcagc acatacagac atacgccgat cgaagactca gccaaggaat 2400 acagaacatt aatgccgatt ttcatcaatc tatcaagatt attagaaaat caatatacac 2460 aaattgcaga ttatcagagg ggcttaatca cacacaaata gtacatgaca tgaaggggag 2520 ggcattacca tgccgttaat catcgttgca cttgggatct tagcattact atttctgatt 2580 atgggcttaa aattaaacac atttatttcc ctgctggtcg tatcgttcgg cgtggcactg 2640 gcactcggga tgccgttcga taaagttgtc agctccatcg aagcaggaat aggggggact 2700 cttggccaca tcgcgctcat cttcggactc ggtgcgatgc tgggcaaact gatcgcggat 2760 tcaggaggcg cacagcgcat tgcgatgacg ctcgtcaaca aattcggtga gaaaaatatt 2820 caatgggccg ttgtcattgc ctcattcatt attgggattg cgttattttt tgaagtagga 2880 ctggttctat taattccgat tgtctttgcg atttcaagag aattgaagat ttctatttta 2940 tttctcggaa tcccgatggt cgcggcacta tccgtcacac acggtttcct gccgccgcac 3000 ccgggaccta cggcgatcgc cggtgagtat ggcgcaaaca ttggagaagt gctgctgtac 3060 ggctttatcg ttgctgttcc gacagtgctc attgcagggc cgttatttac aaagttcgcg 3120 aaaaaaatcg ttcctgcatc atttgcgaaa aacggcaata ttgcatcact cggcacacaa 3180 aaaacattta accttgaaga aacacccggc tttggaatca gtgtctttac tgcaatgctc 3240 ccgattatca tcatgtcggt cgcgaccatt atcgacctgc ttcaagaaac aatcgggttt 3300 gcagataacg gagttttagc ttttatcaga ttgattggaa acgcatcgac tgctatgatt 3360 atttcgttat tggtcgcagt ctatacaatg ggcatcaagc gcaacattcc agtcaaaacc 3420 gtgatggatt cttgttcaac tgccatttca caaatcggca tgatgctttt gatcatcgga 3480 ggaggcggcg ccttcaaaca agtgctgatc aacggcggtg tcggcgatta cgtagcagac 3540 ttattcaaag gaacggcatt atcgccaatc attctggcat ggctcatcgc agcgatcctg 3600 cgcatttctc taggatcggc gactgttgcc gcactaagca caacaggtct ggtcattccg 3660 ttattgggcc attctgatgt taacctggcg ttagtcgtac tcgcaacagg agccggaagt 3720 gtcatcgctt cacacgtcaa tgacgccggc ttctggatgt tcaaggaata cttcggatta 3780 agcatgaaag aaacattcgc cacatggaca ttgctggaaa ccattatttc cgttgctgga 3840 ctgggattca ttttattgtt aagtttagtt gtatgaaatt aagaaggagc tgtaacacat 3900 gttcaattcg attggtgtca taggcttagg cgtaatggga agcaacatcg ccttaaacat 3960 ggcaaataaa ggcgaaaacg tcgctgtcta taattacacc agagatttaa cggaccagct 4020 tatccaaaag ctggatggac aatctctcag cccgtattac gagcttgagg actttgttca 4080 atcgttagaa aaaccaagaa aaatcttttt gatggtcaca gcgggaaaac ccgtagattc 4140 cgtcatccaa tcattaaagc ctttgcttga agaaggcgac gtcatcatgg acggaggaaa 4200 ctcccactat gaagacacag aaagaagata tgacgagctg aaggaaaaag ggatcggcta 4260 cctgggagtc ggcatttccg gcggtgaagt cggtgcgtta acagggcctt ccatcatgcc 4320 gggcggagat cgcgacgtct atgagaaagc cgctcctatc ctgacgaaaa tcgcagccca 4380 agtcggagat gacccttgct gtgtctatat cggaccaaaa ggggcagggc actttacaaa 4440 aatggtgcac aacggcattg aatatgccga catgcagctg attgcagaag cttatacgtt 4500 tctaagagaa acgcttcgtc tgccgctaga tgaaattgca tctatttttg aaacatggaa 4560 tcaaggtgag ctgaaaagct atttaataga gattacagct gagattttac gcaaaaaaga 4620 cgaaaaaaca ggacagcctc tgatcgatgt catccttgat aaaaccggcc aaaaaggcac 4680 cggaaaatgg acgagcatgc aggcgattga taacggcatc ccgtccacga tcatcacaga 4740 gtccttgttc gcccgctact tgtcatcttt aaaagaagaa cggatggcag ctcaagatgt 4800 attagcaggc ccggaagccg aagaaaaaca cttggataaa gacacttgga ttgaatacgt 4860 cagacaggct ctttacatgg ggaaagtatg cgcctatgca caaggctttg cccaatataa 4920 gatgtcatct gagctttacg gctggaatct gccgctcaaa gacatcgcct tgattttccg 4980 aggcggctgc atcatccgcg ctgatttcct aaacgtgatc agcgaagcat tcagtgagca 5040 gccaaatctg gctaacctgc tgatcgcgcc ttatttcaca gataagctcc atgcctatca 5100 aacaggcctg cgaaaagtcg tttgcgaggg tatcagcacc ggaatctctt tcccatgctt 5160 aactaccgcg ctctcttatt acgacggcta ccgcacaggg cgttccaatg cgaacctctt 5220 gcaggcacag cgcgattact ttggcgctca cacgtacgaa cggactgata tggacggcgt 5280 cttccatacg aattggtctg aataacctgt attaaaaaca cggtcagttt caactgaacc 5340 gtgttttttt cttctatccc aaacaacaga agacataagt tatgttttaa atatcataaa 5400 cagtgataca ataatagcta gaaacaatcc ccaagatcat catgtgacag cagattgctg 5460 ttccgttcta ttctgctttt gg 5482 // |
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