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EBI DbfetchID J01675; SV 1; linear; genomic DNA; STD; UNC; 2234 BP. XX AC J01675; XX DT 13-JUN-1985 (Rel. 06, Created) DT 15-JAN-2007 (Rel. 90, Last updated, Version 4) XX DE Plasmid RI13 from E.coli, EcoRI endonuclease and methylase genes, complete DE cds. XX KW EcoRI endonuclease; endonuclease; methylase; modification enzyme; KW restriction endonuclease. XX OS Plasmid RI13 OC other sequences; plasmids. OG Plasmid RI13 XX RN [1] RP 20-2233 RX PUBMED; 6257701. RA Newman A.K., Rubin R.A., Kim S.-H., Modrich P.; RT "DNA sequences of structural genes for Eco RI DNA restriction and RT modification enzymes"; RL J. Biol. Chem. 256(5):2131-2139(1981). XX RN [2] RP 1-2234 RX PUBMED; 6257703. RA Greene P.J., Gupta M., Boyer H.W., Brown W.E., Rosenberg J.M.; RT "Sequence analysis of the DNA encoding the Eco RI endonuclease and RT methylase"; RL J. Biol. Chem. 256(5):2143-2153(1981). XX CC [2] reports 61 bases 5' to base 1 that originate in the cloning CC vector. XX FH Key Location/Qualifiers FH FT source 1..2234 FT /organism="Plasmid RI13" FT /plasmid="RI13" FT /mol_type="genomic DNA" FT /db_xref="taxon:2500" FT CDS 342..1175 FT /codon_start=1 FT /transl_table=11 FT /note="EcoRI endonuclease" FT /db_xref="GOA:P00642" FT /db_xref="InterPro:IPR004221" FT /db_xref="InterPro:IPR011335" FT /db_xref="InterPro:IPR011336" FT /db_xref="InterPro:IPR018131" FT /db_xref="PDB:1CKQ" FT /db_xref="PDB:1CL8" FT /db_xref="PDB:1ERI" FT /db_xref="PDB:1QC9" FT /db_xref="PDB:1QPS" FT /db_xref="PDB:1QRH" FT /db_xref="PDB:1QRI" FT /db_xref="PDB:2OXV" FT /db_xref="UniProtKB/Swiss-Prot:P00642" FT /protein_id="AAA26371.1" FT /translation="MSNKKQSNRLTEQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKKA FT LSNEYPQLSFRYRDSIKKTEINEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYGEW FT RVVLVAEAKHQGKDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSESHF FT PYVLFLEGSNFLTENISITRPDGRVVNLEYNSGILNRLDRLTAANYGMPINSNLCINKF FT VNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVLGRDLFEQLTSK" FT CDS 1205..2185 FT /codon_start=1 FT /transl_table=11 FT /note="EcoRI methylase" FT /db_xref="GOA:P00472" FT /db_xref="InterPro:IPR002052" FT /db_xref="UniProtKB/Swiss-Prot:P00472" FT /protein_id="AAA26372.1" FT /translation="MARNATNKLLHKAKKSKSDEFYTQYCDIENELQYYREHFSDKVVY FT CNCDDPRVSNFFKYFAVNFDNLGLKKLIASCYVENKEGFSSSEAAKNGFYYEYHKENGK FT KLVFDDISVSSFCGDGDFRSSESIDLLKKSDIVVTNPPFSLFREYLDQLIKYDKKFLII FT ANVNSITYKEVFNLIKENKIWLGVHLGRGVSGFIVPEHYELYGTEARIDSNGNRIISPN FT NCLWLTNLDVFIRHKDLPLTRKYFGNESSYPKYDNYDAINVNKTKDIPLDYNGVMGVPI FT TFLHKFNPEQFELIKFRKGVDEKDLSINGKCPYFRILIKNKRLQK" XX SQ Sequence 2234 BP; 745 A; 307 C; 466 G; 716 T; 0 other; cctggagcgg gaacgccagc tggaaataca ggaacgcaca ctggatggtc cttcgttgtc 60 gctgtgatgg cgaaactatg aaaaatggca ggttcggtgg attttgacgg gctaatgtgg 120 tctgcaccat ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgatcgcgg 180 cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgatgaacg 240 cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc aaacgtatgt taatctatcg 300 acatatgtaa ctttataaaa taacagtgga aacatggatt catgtctaat aaaaaacagt 360 caaataggct aactgaacaa cataagttat ctcaaggtgt aattgggatt tttggggatt 420 atgcaaaagc tcatgatctc gctgttggtg aggtttcaaa attagtaaag aaagctctta 480 gcaacgaata ccctcaatta tcatttcgat atagagatag tataaagaaa acagaaataa 540 atgaagcttt aaaaaaaatt gaccctgatc ttggcggtac tttatttgtt tcaaattcca 600 gcatcaaacc tgatggtgga attgtagagg tcaaagatga ttatggtgaa tggagagttg 660 tacttgttgc tgaagccaaa caccaaggta aagatattat aaatataagg aatggtttgt 720 tagttgggaa aagaggagat caagatttaa tggctgctgg taatgctatc gaaagatctc 780 ataagaatat atcagagata gcgaatttta tgctctctga gagccacttt ccttacgtcc 840 ttttcttaga ggggtctaac tttttaacag aaaatatctc aataacaaga ccagatggaa 900 gggttgttaa tcttgagtat aattctggta tattaaatag gttagatcga ctaactgcag 960 ctaattatgg aatgcctata aatagtaatc tatgtattaa caaatttgta aatcataaag 1020 acaaaagcat tatgctacaa gcagcatcta tatatactca aggagatggg agggagtggg 1080 attcgaaaat catgtttgaa ataatgtttg atatatcaac gacttcgctc agagtgttgg 1140 ggcgtgactt gtttgaacag cttacatcta agtgatattt tttattttaa taaggtttta 1200 attaatggct agaaatgcaa caaacaagtt actgcacaaa gctaaaaaat cgaaaagcga 1260 cgaattttac actcagtatt gtgatattga gaacgaactg caatactaca gagagcactt 1320 ctctgataag gttgtttatt gcaattgtga tgatcctaga gtaagcaatt tctttaaata 1380 ttttgcagtg aattttgata atcttggctt gaaaaagtta atagcatctt gctatgtaga 1440 gaataaagaa ggtttttcta gtagcgaagc cgcgaagaac ggattttact atgaatatca 1500 taaagaaaat ggaaagaaat tagtttttga tgatattagt gtttcttctt tctgtggcga 1560 tggcgatttt cgcagttcgg agagcattga tctgctaaaa aaatcagata ttgttgttac 1620 gaatcctcca ttctcgttat ttagagagta tcttgatcaa ctaattaagt atgataagaa 1680 attccttata attgctaatg ttaattcaat aacatataaa gaggtgttta atctaattaa 1740 ggaaaataag atttggcttg gggttcatct cgggagaggt gtttctggat ttattgttcc 1800 agagcattat gaattatatg gtactgaggc gagaattgat tctaatggta atagaattat 1860 ctcgccaaac aactgcttat ggctaactaa cctagatgtc tttattaggc ataaagactt 1920 gcctcttaca agaaaatatt ttgggaatga aagttcatat ccaaaatatg ataattatga 1980 tgctataaat gtaaacaaaa caaaggatat tccattagat tacaatgggg ttatgggggt 2040 tcctatcaca ttcttgcata agtttaaccc tgagcaattt gagttaataa aatttagaaa 2100 gggtgttgat gaaaaagatt tgtctataaa tggtaaatgc ccttatttca gaattttgat 2160 aaaaaacaaa cgattacaaa agtaattgat gtttgttagt tttttcttga gatcattagc 2220 ttcgtcgtaa gctt 2234 // |
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