EBI Dbfetch

ID   DQ489531; SV 1; linear; mRNA; STD; MAM; 1241 BP.
XX
AC   DQ489531;
XX
DT   13-MAY-2006 (Rel. 87, Created)
DT   13-MAY-2006 (Rel. 87, Last updated, Version 1)
XX
DE   Canis familiaris dual specificity mitogen activated protein kinase kinase 2
DE   mRNA, complete cds.
XX
KW   .
XX
OS   Canis lupus familiaris (dog)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
XX
RN   [1]
RP   1-1241
RA   Guerard K.-P., Noel J.;
RT   "Constitutive activation of MEK1/2 in an invasive variant of
RT   MSV-transformed MDCK cell line";
RL   Unpublished.
XX
RN   [2]
RP   1-1241
RA   Guerard K.-P., Noel J.;
RT   ;
RL   Submitted (12-APR-2006) to the EMBL/GenBank/DDBJ databases.
RL   Physiology, University of Montreal, Edouard-Montpetit, Montreal, Qc H3T
RL   1J4, Canada
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1241
FT                   /organism="Canis lupus familiaris"
FT                   /mol_type="mRNA"
FT                   /cell_line="MDCK (Madin Darby canine kidney cell)"
FT                   /PCR_primers="fwd_seq: gatgctggcccggaggaag, rev_seq:
FT                   cactgtcacacggcggtgc"
FT                   /db_xref="taxon:9615"
FT   CDS             18..1220
FT                   /codon_start=1
FT                   /product="dual specificity mitogen activated protein kinase
FT                   kinase 2"
FT                   /note="ERK activator kinase 2; MAPK/ERK kinase 2; MEK2"
FT                   /db_xref="GOA:Q1HG70"
FT                   /db_xref="InterPro:IPR002290"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q1HG70"
FT                   /protein_id="ABF47220.1"
FT                   /translation="MLARRKPVLPALTINPAIAEGPSPTSEGASEANLVDLQKKLAELE
FT                   LDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMARKLIH
FT                   LEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAK
FT                   RIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDS
FT                   MANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVELSIGRYPIPPPDAKELEAIFGR
FT                   PMVDGIEGEPHSISPRPRPPGRPISGHGTDSRPAMAIFELLDYIVNEPPPKLPNGVFTQ
FT                   DFQEFVNKCLIKNPAERADLKMLMSHTFIKRSEVEEVDFAGWLCKTLRLNQPSTPTRTA
FT                   V"
XX
SQ   Sequence 1241 BP; 287 A; 357 C; 371 G; 226 T; 0 other;
     ttgcaagctt ggatccgatg ctggcccgga ggaagccggt gctgccggcg ctcactatca        60
     accccgccat cgccgagggc ccgtccccca ccagcgaggg cgcctccgaa gcaaacttgg       120
     tggacctcca gaagaagcta gcagagctag aacttgatga gcagcagaag aagcgactgg       180
     aagccttcct cacccagaag gccaaggtcg gggaactcaa ggatgatgac ttcgaacgga       240
     tctcagagct gggagccggg aacggcgggg tggtcaccaa ggtccagcac agaccttctg       300
     gcctcatcat ggcgagaaag ctgatccacc tggaaatcaa gccggccatc cggaaccaga       360
     tcatccgcga gctgcaggtg ctgcacgagt gtaactcccc gtacatcgtg ggcttctacg       420
     gggccttcta cagcgacggc gagatcagca tctgcatgga gcatatggat ggcggctctc       480
     tggaccaggt gttgaaggaa gccaagagaa tccctgagga gatcctgggg aaggtcagca       540
     ttgcggttct ccggggtttg gcgtatctcc gggagaagca ccagatcatg caccgagacg       600
     tgaaaccatc caacatcctc gtcaactccc gaggggagat taagctgtgt gattttgggg       660
     tgagcggcca gctcatcgac tccatggcca actccttcgt gggaactcgg tcctacatgt       720
     ctccagagcg gttgcagggc actcattact cagtgcagtc ggacatctgg agcatgggcc       780
     tgtccctggt ggagctgtcc attggaaggt accccatccc gccaccagat gccaaggagc       840
     tagaggccat cttcggccgg cccatggttg acgggataga aggagagcct cacagcatct       900
     caccacggcc gagaccccct ggacgcccca tcagcggtca tgggacggac agccggccag       960
     ctatggccat ctttgagctg ctggactata ttgtgaacga gccgcctccc aagctgccca      1020
     atggtgtctt cacccaggac ttccaggagt ttgtaaataa atgcctaata aagaacccag      1080
     cagagcgagc agatctgaag atgctcatga gccacacgtt catcaagcgg tccgaggtgg      1140
     aagaggtgga ttttgccggc tggttgtgta aaacgctgcg gctgaatcag cccagtacgc      1200
     ccacgcgcac cgccgtgtga cagtgctccg agtctagaga a                          1241
//