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EBI DbfetchID DQ393658; SV 1; linear; genomic DNA; STD; PRO; 14688 BP. XX AC DQ393658; XX DT 02-APR-2006 (Rel. 87, Created) DT 05-APR-2006 (Rel. 87, Last updated, Version 2) XX DE Streptococcus thermophilus strain LY03 eps gene cluster, complete sequence. XX KW . XX OS Streptococcus thermophilus OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus. XX RN [1] RP 1-14688 RA de Vin F., Herman L., De Vuyst L.; RT "Biodiversity of eps gene clusters and their glycosyltransferases in RT Streptococcus thermophilus"; RL Unpublished. XX RN [2] RP 1-14688 RA de Vin F., Herman L., De Vuyst L.; RT ; RL Submitted (07-FEB-2006) to the EMBL/GenBank/DDBJ databases. RL Department of Animal Product Quality and transformation Technology, RL Agricultural Research Centre, Brusselsesteenweg 370, Melle 9090, Belgium XX FH Key Location/Qualifiers FH FT source 1..14688 FT /organism="Streptococcus thermophilus" FT /strain="LY03" FT /mol_type="genomic DNA" FT /db_xref="taxon:1308" FT gene <1..748 FT /gene="deoD" FT CDS <1..499 FT /codon_start=2 FT /transl_table=11 FT /gene="deoD" FT /product="DeoD" FT /function="purine nucleoside phosphorylase" FT /note="COG0813" FT /db_xref="GOA:Q1WCT9" FT /db_xref="InterPro:IPR018017" FT /db_xref="UniProtKB/TrEMBL:Q1WCT9" FT /protein_id="ABD96520.1" FT /translation="YARELIVDYGVKKLIRVGTAGSLNENVHVRELVLAQAAATNSNII FT RNDWPQYDFPQIASFNLLDKAYHIAKNFGMTTHVGNVLSSDVFYSNYFEKNIELGKWGV FT KAVEMEAAALYYLAAQHQVDALAIMTISDSLVNPDEDTTAEERQNTFTDMMKVGLETLI FT AD" FT terminator 738..748 FT /gene="deoD" FT misc_feature 770..14150 FT /note="eps gene cluster" FT gene 770..2302 FT /gene="epsA" FT -35_signal 770..775 FT /gene="epsA" FT -10_signal 793..798 FT /gene="epsA" FT CDS 848..2302 FT /codon_start=1 FT /transl_table=11 FT /gene="epsA" FT /product="EpsA" FT /function="exopolysaccharide regulation" FT /note="COG1316; LytR transcriptional regulator" FT /db_xref="GOA:Q1WCT8" FT /db_xref="InterPro:IPR004474" FT /db_xref="UniProtKB/TrEMBL:Q1WCT8" FT /protein_id="ABD96519.1" FT /translation="MSSRTNRKQKHTSNGSWGMVNVGLTILYAILALVLLFTMFNYNFL FT SFRFLNIIITIGLLVVLAISIFLQKTKKLPLVTTVVLVIFSLVSLVGIFGFKQMIDITN FT RMNQTAAFSEVEMSIVVPKESDIKDVSQLTSVQAPTKVDKNNIEILMSALKKDKKVDVK FT VDDVASYQEAYDNLKSGKSKAMVLSGSYASLLESVDSNYASNLKTIYTYKIKKKNSNSA FT NQVDSRVFNIYISGIDTYGPISTVSRSDVNIIMTVNMNTHKILLTTTPRDAYVKIPGGG FT ADQYDKLTHAGICGVETSEQTLEDLYGIKLDYYARINFTSFLKLIDQLGGVTVHNDQAF FT TQEKFDFPVGDIQMNSEQALGFVRERYNLDGGDNDRGKNQEKVISAILNKLASLKSVSN FT FTSIVNNLQDSVQTNMSLNTINALANTQLESGSKFTVTSQAVTGTGSTGQLISYAMPNS FT SLYMMKLDNSSVESASQAIKKLMEEK" FT gene 2303..3034 FT /gene="epsB" FT CDS 2303..3034 FT /codon_start=1 FT /transl_table=11 FT /gene="epsB" FT /product="EpsB" FT /function="exopolysaccharide synthesis" FT /note="COG4464; CapC capsular polysaccharide biosynthesis FT protein" FT /db_xref="GOA:Q56039" FT /db_xref="InterPro:IPR016667" FT /db_xref="UniProtKB/TrEMBL:Q56039" FT /protein_id="ABD96521.1" FT /translation="MIDVHSHIVFDVDDGPETLEESLDLIGESYAQGVRKIVSTSHRRK FT GMFETPEDKIFANFKKVKAEAEALYPDLTIYYGGELYYTSDIVEKLEKNLIPRMHNTQF FT ALIEFSARTSWKEIHSGLSNVLRAGVTPIVAHIERYDALEENADRVREIINMGCYTQVN FT SSHVLKPKLFGDKDKVRKKRVRFFLEKNLVHMVASDMHNLGPRPPFMKDAYEIVKKNYG FT SKRAKNLFIENPKTLLENQYL" FT gene 3043..3735 FT /gene="epsC" FT CDS 3043..3735 FT /codon_start=1 FT /transl_table=11 FT /gene="epsC" FT /product="EpsC" FT /function="exopolysaccharide chain length determination" FT /note="COG3944; capsular polysaccharide biosynthesis FT protein" FT /db_xref="GOA:Q1WCT6" FT /db_xref="InterPro:IPR005701" FT /db_xref="UniProtKB/TrEMBL:Q1WCT6" FT /protein_id="ABD96522.1" FT /translation="MNQDNTKSDEIDVLALLHKLWTKKLLILFTAFYFAVFSFLGTYFF FT IQPTYTSTTRIYVVNQATDNKNLSAQDLQAGTYLANDYKEIIASNDVLSEVIKDEKLNL FT SEAELSKMVSVNIPTDTRLISISVNAKTGQDAQTLANKVREVASKKIKKVTKVEDVTTL FT EEAKLPESPSSPNIKLNVLLGAVLGGFLAVVGVLVREILDDRARRPEDVEDALGMTLLG FT IVPDTDKI" FT gene 3745..4494 FT /gene="epsD" FT CDS 3745..4494 FT /codon_start=1 FT /transl_table=11 FT /gene="epsD" FT /product="EpsD" FT /function="membrane translocation of the exopolysaccharide" FT /note="COG0489; Mrp ATPases involved in chromosome FT partitioning" FT /db_xref="GOA:Q56041" FT /db_xref="InterPro:IPR005702" FT /db_xref="UniProtKB/TrEMBL:Q56041" FT /protein_id="ABD96523.1" FT /translation="MPLLKLVKSKVDFAKKTEEYYNAIRTNIQFSGAQMKVIAISSVEA FT GEGKSMISVNLAISFASVGLRTLLIDAETRNSVLSGTFKSNEPYKGLSNFLSGNADLNE FT TICQTDISGLDVIASGPVPPNPTSLLQNDNFRHLMEVARSCYDYVIIDTPPVGLVIDAV FT IIAHQADASLLVTEAGKIKRRFVTKAVEQLVESGSQFLGVVLNKVDMTVDKYGFYGSYG FT SYGEYGKKSDQKEGHSRAHRRRKVGWN" FT gene 4547..5230 FT /gene="epsE" FT CDS 4547..5230 FT /codon_start=1 FT /transl_table=11 FT /gene="epsE" FT /product="EpsE" FT /function="phosphogalactosyltransferase" FT /note="Pfam02397; sugar transferase" FT /db_xref="InterPro:IPR003362" FT /db_xref="UniProtKB/TrEMBL:Q1WCT4" FT /protein_id="ABD96524.1" FT /translation="MSQAIEEISDVMTYSELTSHKPKIIYSLIKRIGDILVSSIGLIIL FT IPLFLIVALIMKCSEPTAPIFFSHIRNGKNGKKFKMYKFRTMCQDAESILMKDTELFAK FT FKANGYKLETHEDPRITKIGGILRKTSIDELPQLINVFLGQMSLVGPRPLPDREIIEYG FT DNQEKFLSVKPGMTGWWQVSGRSTIGYPERCHLELYYVEKCCFTFDVLILLKTIGIVLK FT RVGAR" FT gene 5394..6353 FT /gene="epsF" FT CDS 5394..6353 FT /codon_start=1 FT /transl_table=11 FT /gene="epsF" FT /product="EpsF" FT /function="glycosyltransferase" FT /note="Pfam00534; glycosyl transferases group 1" FT /db_xref="GOA:Q56043" FT /db_xref="InterPro:IPR001296" FT /db_xref="UniProtKB/TrEMBL:Q56043" FT /protein_id="ABD96525.1" FT /translation="MNEQVTFILCDFLVREIKPKYDLLAYQFISKKIKEIKPDIVHCHS FT SKAGVIGRLAAKRRGVKKIFYTPHAYSFLAPEFSGKKKFLFVQIEKFLSRFATTKIFCV FT SIAEMQAALEVNLDKTDKFQVIYNGLPEIDLPSKETIRAQLGLEKAAVVIGNNAKMSEQ FT KNPMFFMEIARKMIRQNANWHFVWVGDGQLMPLFQSFIKQNGLEGNIHLLGERPDSEIV FT VTAYDIFLTTSQYEGLPYAPIEAMRAGVPILATKVVGNSELVIEGKNGYLIDLEWSKSV FT EEKLYKAAKIDAQMIKADFRQRFAIDQILKQIETIYLA" FT gene 6921..8039 FT /gene="epsG" FT CDS 6921..8039 FT /codon_start=1 FT /transl_table=11 FT /gene="epsG" FT /product="EpsG" FT /function="glycosyltransferase" FT /note="COG0438; RfaG glycosyltransferase" FT /db_xref="GOA:Q56044" FT /db_xref="InterPro:IPR001296" FT /db_xref="UniProtKB/TrEMBL:Q56044" FT /protein_id="ABD96526.1" FT /translation="MKKISILHFSQVSGGGVEKYIKLFLKYSDVTKFNNYLVAPNLENY FT DEFNGYLKMSVNFNMEQTFSPLKIFKNVFFIRSVLKKINPDIVYLHSTFAGVVGRIASI FT GLPTKVVYNPHGWSFKMDNSYLKKLIFKLIEFSLSFLTDKFILISESEYILANHISFNK FT SKFSLINNGVEVITGDSRNEIEEIFPNEDFIIGMVGRLSPPKEFFFFIDFAKKILQIRN FT DTNFIIVGDGELRSEIERMILDNGLGDKIYITGWVDNPRNYIEKFDQAILFSRWEGLSL FT TIAEYMSQKKTILATNIGGINDLITDGETGMLIEVGDLNSAVSKSFELRNNKEVSNQLA FT NNAYNKVVEQFSIEKQMAEIESLFIEMCNNEK" FT gene 8232..8711 FT /gene="epsH" FT CDS 8232..8711 FT /codon_start=1 FT /transl_table=11 FT /gene="epsH" FT /product="EpsH" FT /function="sugar biosynthesis" FT /note="COG0110; WbbJ acetyltransferase'" FT /db_xref="GOA:Q56045" FT /db_xref="HSSP:1KRR" FT /db_xref="InterPro:IPR018357" FT /db_xref="UniProtKB/TrEMBL:Q56045" FT /protein_id="ABD96527.1" FT /translation="MLILKLKFHLKSLFLKWIYRLLYLKKFQFGARLTFRDGFHLLIEK FT SGKVIIGNHVFFNNFCSINAMLSVTIGDDCIFGENVKIYDHNHCYQNKSQPISKQGFST FT AAIQIGRNCWIGSQVTILKGVTIGDNSIIGAGVVVYQDVPENSIVLSNGEIRKRG" FT gene 8717..9691 FT /gene="epsI" FT CDS 8717..9691 FT /codon_start=1 FT /transl_table=11 FT /gene="epsI" FT /product="EpsI" FT /function="glycosyltransferase" FT /note="Pfam00535; glycosyl transferase group 2" FT /db_xref="GOA:Q56046" FT /db_xref="InterPro:IPR001173" FT /db_xref="UniProtKB/TrEMBL:Q56046" FT /protein_id="ABD96528.1" FT /translation="MYLKSLISIVIPVYNVEKYLEKCLQSVQNQTYNNFEVILVNDGST FT DSSLSICEKFVNQDKRFSVFSKENGGMSSARNFGIKKAKGSFITFVDSDDYIVKDYLSH FT LVAGIKSETSIVCSKFFLVDEKGSLLTKKEAPKKKSEVVSIEESIKILLLQQNGYDLAV FT WGKLYPVSFFETISFPEGKLYEDMGTTYKLLKLASEVVFLDAYDYAYVQRPNSIMNSSF FT NLKKLDIIEMVHEMENDILAQFPNLALYVKNRAFAAEVKIFLEIPKEKEFEQAQKQLWH FT DIKKNRKAPFMTKGARLKNRLGASLSFLGKSLFLTIGKQLVDR" FT gene 9781..10863 FT /gene="epsJ" FT CDS 9781..10863 FT /codon_start=1 FT /transl_table=11 FT /gene="epsJ" FT /product="EpsJ" FT /function="exopolysaccharide polymerization" FT /db_xref="UniProtKB/TrEMBL:Q1WCS9" FT /protein_id="ABD96529.1" FT /translation="MVIYFLLFPMIAMIYLMTLLLRQKAQIQKTIFCVLTFGTLGFISA FT SRASSAGTDVTLYENIFKSINYGISAESNWGYVIYNKLIGSVFGYTGHEITAANSVLIT FT ILIGIFIWKVAEHYFVATFLYISLFYYATSFNISRQFIAMGLVLVAISFALDKKVMPWF FT ILTVLATLFHATAIVAFPVYWLTKVHWDVKKTLSIFPITIFASFIFDAILNIFVRFFPH FT YEMYITGTQFNISDQGQGRVVLVKIFILLILFTLFLFYKKSYALISECHQSLIALTTVG FT LSIGIVFYNNILLNRIEMFYSILSIVFIPIAIDYISLKFKQKDAVRLMLTIGILLITLV FT PYYIQVSGNYSGILPYVIQQ" FT gene 10888..11838 FT /gene="epsK" FT CDS 10888..11838 FT /codon_start=1 FT /transl_table=11 FT /gene="epsK" FT /product="EpsK" FT /function="exopolysaccharide membrane translocation" FT /note="Pfam02485; core-2/I-branching enzyme" FT /db_xref="GOA:Q8KHW5" FT /db_xref="InterPro:IPR003406" FT /db_xref="UniProtKB/TrEMBL:Q8KHW5" FT /protein_id="ABD96530.1" FT /translation="MEDRKKQAILILSHRNTLALKSTIELLDSQYFDFFLHIDKKSRIQ FT DFFYLKKITKFSTIHFSERKNVHWGGFSMVEAMFALLECARDTGEYSYFHFLSGDDMPI FT KDNEIVFNFFENSYPKNFIDILDFENVNKNSYFYEPPEMIEERVKYYYPHMDILNRKGT FT NFIGKKLIYLQKLLKVNRLKNREIEIFKGHQWCSLTNQFVDILLDKEERRVGKSYFSSS FT LIPDECYFQTFAMIKKVEIYQQKNMSARLIDWTRGKPYIWRQDDFFEIMNDKDSMFSRK FT FDENVDRKIIEEIYIKIRGRSTDEANKIKDKRFTK" FT -35_signal 11732..11737 FT /gene="epsK" FT gene 11751..12721 FT /gene="epsL" FT -10_signal 11751..11756 FT /gene="epsK" FT CDS 11798..12721 FT /codon_start=1 FT /transl_table=11 FT /gene="epsL" FT /product="EpsL" FT /function="exopolysaccharide synthesis" FT /note="COG5039; exopolysaccharide biosynthesis protein" FT /db_xref="GOA:Q56049" FT /db_xref="InterPro:IPR007345" FT /db_xref="UniProtKB/TrEMBL:Q56049" FT /protein_id="ABD96531.1" FT /translation="MKQIKSKIRDLQNNFTYVFGKKTFLGRGEAIIIDEPEHGNLGDQA FT IAFAENQFLVNHVSVRDVEHLIESKTISEIKSIKKNIGKKELVFFHGGGNFGTLYLKYE FT RIRRLAVSKLPFNKMILFPQSISFEDSRFGQKQLNKSKKIYSQNTNFILTAREPKSYGL FT MKKCFPYNKVILTPDIVLSFKFEVTISDTHIGKEKDSVITYENRQHYLEIKWDEIAQHE FT VALTDRLHGMIFSYITGTPCVVLANNNHKIEGTYKHWLNEVNYIRFIENPTVENILDAI FT NDLKQIEPHYIDLSDKFQPLIDAIKG" FT gene 12729..14194 FT /gene="epsM" FT CDS 12729..14150 FT /codon_start=1 FT /transl_table=11 FT /gene="epsM" FT /product="EpsM" FT /function="exopolysaccharide synthesis" FT /note="COG2244; RfbX membrane protein involved in the FT export of O-antigen and techoic acid" FT /db_xref="GOA:Q1WCS6" FT /db_xref="InterPro:IPR002797" FT /db_xref="UniProtKB/TrEMBL:Q1WCS6" FT /protein_id="ABD96532.1" FT /translation="MNKYKKLLSNSLVFTIGNLGSKLLVFLLVPLYTYAMTPQEYGMAD FT LYQTTANLLLPLITMNVFDATLRFAMEKSMTKESVLTNSLVVWCFSAVFTCLGACIIYA FT LNLSNKWYLALLLTFNLFQGGQSILSQYARGIGKSKIFAAGGVILTFLTGALNILFLVY FT LPLGITGYLMSLVLANVGTILFFAGTLSIWKEISFKIIDKKLIWQMLYYALPLIPSSIL FT WWLLNASSRYFVLFFLGAGANGLLAVATKIPSIISIFNTIFPQAWQISAIEEYDSHQKS FT KYYSDVFHYLATFLLLGTSAFMIVLKPIVEKVVSSDYASSWQYVPFFMLSMLFSSFSDF FT FGTNYIAAKQTKGVFMTSIYGTIVCVLLQVVLLPIIGLDGAGLSAMLGFLTTFLLRVKD FT TQKFVVIQIKWRIFISNLLIVLAQILCLFYLPSEFLYFGLALLFCGMLVVNQRTILYII FT MALKIKNKTFGMKSS" FT CDS complement(14226..>14688) FT /codon_start=2 FT /transl_table=11 FT /product="hypothetical protein" FT /note="Orf14.9; Pfam04892, VanZ like family" FT /db_xref="InterPro:IPR006976" FT /db_xref="UniProtKB/TrEMBL:Q1WCS5" FT /protein_id="ABD96533.1" FT /translation="LINHFDYTTLTLILLLTLIIWLFGIQYHLKYFSVIYLYLAFSVYL FT LLLFMVLFTKITDFQAMSLNPFDFIKADPRTIQEAVLNIIYFIPLGGLYCINTDFKQFV FT IVSLVTLLVIETVQFIFYLGTFAISDIILNFLGCLIGYYCCWEIKRRLS" XX SQ Sequence 14688 BP; 4956 A; 2099 C; 2807 G; 4826 T; 0 other; ctatgcacgt gagctcattg ttgactacgg tgtcaaaaaa ttaattcgcg taggaactgc 60 cggttctttg aatgagaatg ttcacgttcg tgagttggtt ctagcacaag cagcagcaac 120 taactctaat attattcgta atgactggcc tcaatacgac tttcctcaaa ttgcaagttt 180 caatctcttg gataaggctt atcacatcgc caaaaatttt ggtatgacaa cacatgttgg 240 taatgttcta tcttctgatg ttttctactc aaattatttt gaaaaaaata ttgaacttgg 300 gaaatggggc gtgaaggctg tcgaaatgga agcagcagca ctttactacc ttgcagccca 360 acaccaagta gatgctctgg ctattatgac tatttctgat agtttggtaa atccagatga 420 ggacacaaca gctgaggaac gtcaaaacac tttcactgat atgatgaaag ttggtcttga 480 aacactaatt gcagactagt ttgtaaaagg acgccatttg gtcgtccttt tgtgttgtag 540 ctaatatctg ttcgaagtga taataagtta aaatttttca aactactaga aaaaataaaa 600 atatttggaa gaagaagact tataataaat aggtaaatat ctgacaattt aaagtttaac 660 tactaaaaat gtaaaagata gttcacaata taatggaaaa tgatataaat taaatgattg 720 atatcataat gaaaaacgtt ttcttatttt tttgaaaaaa gaatgacaat tgagatgagg 780 ttgtattaat gttataataa taataataat ggggaatacc taattttaat ttttaggagc 840 aatttatatg agttcgcgta cgaatcgtaa gcaaaagcat acgagtaatg gatcgtgggg 900 gatggtcaac gttgggttga ccatcctgta tgctatttta gcattggtct tattattcac 960 catgttcaat tataatttcc tatcctttag gtttttgaac atcattatca ccattggttt 1020 gttggtagtt cttgctatta gcatcttcct tcagaagact aagaaattac cactagtgac 1080 aacggttgta ctggttatct tctcgctagt ttctctggtt ggtatttttg gttttaaaca 1140 aatgattgac atcactaacc gtatgaatca gacagcagca ttttctgaag tagaaatgag 1200 catcgtggtt cctaaggaaa gtgacatcaa agatgtgagc cagcttacta gcgtacaggc 1260 acctactaag gttgataaga acaatatcga gatcttgatg tcagctctca aaaaagataa 1320 aaaagttgat gttaaagttg atgatgttgc ctcatatcaa gaagcttatg ataatctcaa 1380 gtctggcaaa tctaaagcta tggtcttgag tggctcttat gctagcctat tagagtctgt 1440 cgatagtaat tatgcttcaa atctaaaaac aatttatact tataaaatta aaaagaagaa 1500 tagcaactct gcaaaccaag tagattcaag agtcttcaat atttatatta gtggtattga 1560 tacctacggt ccgatttcaa cagtgtcacg ttcagatgtc aatatcatta tgacagtaaa 1620 catgaataca cataagattc tcttgacgac tactccacgt gatgcatacg ttaagattcc 1680 tggtggtggg gcagaccagt atgataaatt aacccacgca ggtatttgtg gcgttgaaac 1740 atctgaacaa actctagaag atctttatgg tattaagctt gattactatg cacgaattaa 1800 cttcacatct ttccttaagt tgattgacca acttggtggt gtgacagtcc ataatgatca 1860 agctttcaca caagagaagt ttgatttccc ggttggagat atccaaatga attcagagca 1920 agcacttgga tttgttcgtg aacgctataa tttagatggc ggagataatg accgtggtaa 1980 aaaccaggag aaagttattt ctgcgatttt aaacaagttg gcttctctaa aatctgtatc 2040 aaactttact tcaatcgtta ataatctcca agactctgtc caaacgaata tgtctttgaa 2100 taccattaac gctttggcta atacacaact tgaatcaggt tctaaattta cggtgacttc 2160 tcaagcagta acaggtacag gttcaaccgg acaattgatc tcttatgcga tgccaaattc 2220 tagtctttac atgatgaaac tagataattc gagtgtggaa agtgcctctc aagctatcaa 2280 aaaattgatg gaggaaaaat aagtgattga cgttcactca catattgttt ttgatgttga 2340 tgatggtcct gaaactttag aagaaagttt agacctcatt ggtgaaagtt acgcccaggg 2400 ggtacgtaag attgtttcaa catcccatcg tcgtaagggg atgtttgaga ctccagagga 2460 taaaattttt gccaacttta aaaaagtaaa agcagaagca gaagcacttt atccagactt 2520 aactatttat tatggaggtg aactttatta cacctcagac attgtggaga aacttgaaaa 2580 gaatctcatt ccgcgcatgc acaacactca atttgcttta attgagttta gtgctcgcac 2640 atcttggaaa gaaattcata gtgggcttag taatgttttg agagcggggg taacgcctat 2700 tgttgctcat attgagcgct atgatgccct cgaagaaaat gctgaccgtg ttcgagaaat 2760 catcaatatg ggctgctata ctcaagtcaa tagctcacat gtcctcaaac caaagctctt 2820 tggagataaa gataaagtaa gaaagaaacg tgttcgcttt ttcttggaga aaaatttggt 2880 tcatatggtt gctagcgaca tgcataatct tgggccgaga ccaccattta tgaaagatgc 2940 ttatgaaatt gttaaaaaga actacggctc caaacgtgct aagaatcttt ttattgaaaa 3000 tcccaaaaca ttactagaaa atcaatattt ataggagata ttatgaatca agataacact 3060 aaaagtgatg aaatcgacgt actagcattg ctacataaac tttggacgaa gaagcttttg 3120 attcttttca cagcttttta tttcgctgtt ttcagtttct taggtactta tttctttatc 3180 caaccaacat atacatcaac aacgcgtatc tatgttgtta atcaggcaac agataataag 3240 aatctttctg ctcaagattt gcaagctggt acctatttgg caaatgacta taaagagatt 3300 attgcatcaa atgatgtatt atcagaagtt attaaagatg aaaaattgaa tttgagtgag 3360 gcagaactgt ctaaaatggt ttcagttaat attcctactg atactcgtct tatttcaatt 3420 tctgttaatg ctaaaactgg tcaagatgcg caaacacttg ccaataaggt tcgtgaagtt 3480 gcttcaaaaa aaatcaagaa ggtgacaaaa gttgaagatg tcacaacgct cgaagaagct 3540 aaattgccag agtcaccatc ttcaccaaat atcaaactta atgtgcttct cggggcagtg 3600 cttggaggat tccttgcagt ggttggtgta ttggtacgtg aaatcctaga tgatcgtgct 3660 cgccgtccag aagatgtgga agatgccctt ggaatgacac ttcttggaat tgtccctgat 3720 acagataaaa tttaaggaga agaaatgcct ttattaaagt tagttaaatc aaaagtagac 3780 tttgctaaaa agacggaaga gtattataac gctattcgca caaatattca attttctggt 3840 gctcagatga aagtgattgc gattagctct gttgaagctg gtgaaggaaa atcaatgata 3900 tctgttaact tggcgatttc atttgctagt gttgggctcc gaacacttct gattgatgcg 3960 gaaacgcgta attctgtttt gtcaggtaca tttaaatcaa atgagcctta taaaggtctt 4020 tcaaatttcc tttcaggaaa tgccgatcta aatgaaacga tttgccaaac tgatatttct 4080 ggtttagatg ttattgcatc tggtcctgtt ccacctaatc caacaagtct tttgcaaaat 4140 gataatttta gacatttgat ggaagttgct cgtagttgtt atgattatgt catcatcgat 4200 acaccaccag ttggtctggt tattgatgca gttattattg cccatcaggc tgatgccagt 4260 cttttggtta cagaagctgg gaaaattaaa cgtcgtttcg taactaaggc cgttgaacaa 4320 ttggtagaaa gtggttctca gttcttaggg gtcgtcctta ataaagttga catgacagtt 4380 gataaatatg gattttatgg ttcttacgga tcatatggcg agtatggaaa aaaatctgac 4440 caaaaagaag gtcattcaag agcacatcgt cgtagaaaag tcggttggaa ttaacgcgtt 4500 agtgtgtttt aagatgtcgt tgggaacgac aagtggaggg aatgagatgt cacaagctat 4560 agaggaaatt tcagatgtta tgacttattc agagctaaca agtcataagc ccaaaattat 4620 ttatagcttg attaagcgga ttggtgatat tttggttagt tctattggtt taattatttt 4680 gataccgcta tttttgatag ttgctttgat catgaaatgc tctgaaccaa cagcacctat 4740 atttttctca catattagaa atggtaaaaa tggcaaaaag ttcaaaatgt ataaatttag 4800 aaccatgtgt caggacgcag aatcgatttt gatgaaagat acggaacttt ttgcaaaatt 4860 taaggcaaat ggttataaac ttgaaacgca tgaagatcct agaattacaa aaatcggtgg 4920 catattaagg aaaacaagta ttgatgaatt gccacaactg attaatgttt ttttaggaca 4980 aatgtcatta gtgggtccac gtccactacc agatagagaa atcattgaat acggtgataa 5040 ccaagaaaaa tttttaagcg ttaaaccagg catgacagga tggtggcaag tttcagggag 5100 aagtactatt gggtatcctg agcggtgtca tcttgagctt tattatgtag aaaagtgttg 5160 ttttactttc gatgttctta tattacttaa gacaattggg attgttttga agagagttgg 5220 agcgcgttag tactgatgaa acaaaaatta ttattgataa tagaagcgat gagtggtgga 5280 gccggtcgtc atgtacaaga cttgattagt catctacctc aagaaaaatt tgatatttat 5340 gtgatttatt caaatcatag aacaaatcct gttttttgga aaaaatagta acgatgaatg 5400 agcaagtaac ttttatttta tgtgattttc tcgtaagaga aattaaaccg aaatatgatt 5460 tgcttgctta tcaatttatt tctaaaaaga ttaaagaaat caaaccagat attgtacatt 5520 gtcacagttc aaaagctggt gttattggtc gtttagctgc caaaagacga ggtgttaaaa 5580 aaatatttta tacgccacat gcttattcgt ttttggcacc tgaatttagt gggaagaaaa 5640 agtttctatt tgttcaaatt gaaaagtttt taagccgatt tgcgacaact aagatatttt 5700 gtgtgtcaat agcggaaatg caagctgctc ttgaagtaaa tctagataaa accgataagt 5760 ttcaggtaat ttataatggt ttgccagaaa ttgatttacc aagcaaagaa acgattcggg 5820 cgcaattagg actggaaaag gcagcagttg ttataggcaa taatgcaaaa atgtcggaac 5880 agaaaaatcc tatgtttttt atggaaattg cccgaaaaat gattagacaa aacgcaaatt 5940 ggcattttgt gtgggtaggt gatggtcagc tgatgccact ttttcaatca tttattaagc 6000 aaaatggact agagggaaat atccatttgc ttggcgagcg tcctgatagt gaaatagttg 6060 tgacagccta tgacatcttc ttgacgactt cccaatatga aggtttacct tatgcaccaa 6120 ttgaagcgat gcgagctggt gtcccgattc ttgcgacaaa agttgttggc aatagtgagc 6180 ttgtgataga gggcaaaaat ggttatttga ttgacttaga gtggtcaaaa tctgtcgaag 6240 aaaaattata taaggcagcg aaaatagatg cacaaatgat taaagcagat tttaggcaaa 6300 ggtttgcgat tgatcagata ttaaagcaaa ttgaaacaat ttatttagct tgaatgaaga 6360 atgaggaggc ataaatgctg attttgaaat taaaatttca tcttaattgg tacacaaacg 6420 aaaaccatta ttacacgtga gtattcgaag acctggaaac gaggcgatga gccgtattat 6480 ccagtgaaca atgatcgtaa caacaaactc tatactgcct ataagcgtct tgccgagcaa 6540 caagagaatg tcattttcgg tggacgtcta ggtcactacc gttactacga tatgcaccag 6600 gtaattggag ctgccttgca gtgtgtcaga aatgaagtga agtaaatctt gatgaagttg 6660 aataacttta agtaatttta tacttaatcc aattgatgaa aatatttttg tatcgattta 6720 tcttctgtaa gaagagtcct aatcgtttaa aaaatgtaca attgagtttt tatattttta 6780 aataaagtta cttttaagtc gtgttataga atatacatga ataggtgtat tagaaaattt 6840 attaatctaa tcctcgaaaa taactgactg taaggaatca agttgtggag tgtaagttgt 6900 caaatggaga ggaaaataat atgaaaaaaa tttcaatttt acacttttcc caagtatcag 6960 gcgggggagt tgaaaagtac ataaaattat ttttaaagta ttctgatgtg acaaaattta 7020 ataattattt agttgcacct aatcttgaaa attatgacga atttaatgga tatttaaaga 7080 tgtctgtcaa ttttaatatg gaacaaactt tttctccgct aaaaatattc aaaaatgtct 7140 tttttattcg tagtgtactc aaaaaaataa acccagatat agtataccta catagtacat 7200 ttgcaggtgt cgtaggtcgt attgcttcaa taggtttgcc aacaaaagta gtatacaatc 7260 ctcacggatg gtccttcaaa atggacaaca gctatttgaa aaagcttatt tttaaattaa 7320 tcgaattttc tttatctttt ttaactgata agtttatttt aatttcggaa tctgagtata 7380 ttttggctaa ccatatttca tttaataaaa gcaagttttc actaattaat aatggtgttg 7440 aagtgattac aggggattca agaaatgaga tagaagagat atttccaaat gaggatttta 7500 taattggcat ggttggcaga ctaagcccac ccaaagagtt tttctttttt attgattttg 7560 caaaaaaaat attacaaatt cgaaacgata ccaactttat tatcgtgggt gatggagagt 7620 tacgaagtga aatagaaaga atgatactag ataatgggtt aggagataaa atctatatta 7680 ctgggtgggt tgataatccg agaaactata tagagaagtt tgatcaagct attctgtttt 7740 ctagatggga gggtcttagc ctaacgattg cggaatatat gtctcagaag aaaacaattt 7800 tagcaacaaa tattggtggc attaatgatt taatcactga tggtgaaaca ggaatgctga 7860 ttgaagttgg agacttgaat tcagcagtat ctaaatcttt cgagctaaga aataataaag 7920 aggtttcgaa tcaattagcg aataacgctt ataataaagt tgttgaacag ttttcgattg 7980 aaaaacagat ggctgagata gaaagtttat ttatagagat gtgtaacaat gagaaataga 8040 gacttaaaga aaatacaggt tatttgattg acttagagtg gtcaaaatct gtcgaagaaa 8100 aattatataa ggcagcgaaa atggatgcac aaatgattaa agcagatttt aggcaaaggt 8160 ttgcgattga tcagatgtta aagcaaatta aaacaattta tttagcttga atgaagaaag 8220 aggaggcata aatgctgatt ttgaaattaa aatttcatct taaatcgtta ttccttaaat 8280 ggatttatcg attactttat ctaaaaaagt ttcagtttgg tgcacgcttg acgtttcgag 8340 atgggtttca tttgttaatt gaaaaatctg ggaaagttat catcgggaat catgtttttt 8400 ttaataactt ttgttcaatt aatgccatgt tatcagtaac gattggtgat gactgtattt 8460 ttggtgaaaa cgttaaaatt tatgatcaca atcattgtta tcaaaataaa agtcaaccta 8520 tttcaaaaca aggtttttca actgctgcta tccagattgg tcgtaactgt tggataggta 8580 gtcaagtgac gattttaaaa ggtgtaacca taggtgataa tagtatcatt ggtgctggtg 8640 tggtagttta tcaagatgtg ccagaaaatt cgattgtttt atctaatgga gaaattagaa 8700 agcgtggcta attaaaatgt atcttaaaag tctaatctct attgttattc cagtatataa 8760 tgtagagaaa tatttagaaa aatgtttgca atctgttcaa aatcagactt acaataattt 8820 tgaagtgatt ttagtgaatg atggctcaac cgattcatca ctttcaatat gcgaaaaatt 8880 tgttaatcag gataaaagat tttctgtttt ttctaaagaa aatggtggta tgtcatctgc 8940 acgaaatttt ggaattaaaa aggctaaagg atcgtttatc acatttgtag atagtgatga 9000 ctacatagta aaagattatc tttctcattt ggtagctggg ataaaaagtg agacctctat 9060 agtttgttca aagttttttc ttgtagatga aaaaggaagt ttattgacta aaaaagaggc 9120 acctaaaaag aaatcagaag tcgtttcaat tgaggaaagt attaaaattc ttctgttgca 9180 acaaaatggc tatgatctcg ctgtctgggg aaaattatac cccgtttctt tctttgaaac 9240 aatttctttc ccagaaggaa aactttacga agatatggga acaacttaca aattactaaa 9300 attggcaagt gaagtggtct tcttggatgc gtatgattat gcctacgtac agcgacctaa 9360 tagtatcatg aatagttctt ttaatttgaa aaagttggat ataatagaaa tggttcatga 9420 aatggaaaac gatatattag cacagtttcc aaatttagca ttatatgtta agaatcgagc 9480 atttgccgcg gaagtgaaaa tctttttaga gattccaaaa gaaaaagaat ttgagcaagc 9540 gcaaaagcaa ctttggcatg atatcaaaaa gaatagaaaa gcaccattta tgacaaaagg 9600 tgctagattg aagaataggc tcggagctag tctgtcgttt ttaggtaaat ctttattttt 9660 gactattggg aagcagttag tagatagata atgatattga aagcgatacg atacaatcgt 9720 aaacttcttt tggtgttgac taggagttag cttgaaattt gaatataaag gaagcaacac 9780 atggtaattt attttttact tttcccgatg atcgcaatga tttatttaat gacattgctc 9840 ttacggcaaa aagcacaaat ccaaaaaacg attttttgtg ttcttacgtt tggtacacta 9900 ggctttattt cagcaagtcg tgcatcaagt gctgggacgg acgttacttt atacgaaaat 9960 atttttaaat ctataaatta cgggataagt gctgagagta attggggata cgtcatctat 10020 aacaagctga ttggtagtgt atttggctat acaggacatg aaatcacggc cgctaattca 10080 gttttgatta caatacttat tggtattttt atttggaaag tagcggaaca ttattttgtt 10140 gcgacgtttt tatacattag cttgttttat tatgctacaa gttttaatat ttcaagacaa 10200 tttattgcca tggggcttgt attggtagca atttcttttg ctttagataa aaaggttatg 10260 ccttggttta tcttgacagt tttggctacc ttatttcatg cgacagcaat cgttgctttt 10320 cctgtctatt ggcttacaaa agtacattgg gatgtgaaaa agacattaag tatttttcca 10380 atcacgattt ttgcaagttt tatttttgat gctattttaa acatttttgt acgttttttc 10440 ccacattatg agatgtatat cactggaaca caatttaata tttcagatca ggggcaggga 10500 cgtgtggttt tggtcaaaat atttatcttg ctcattttgt ttactttatt cttgttttat 10560 aaaaaaagct atgctttgat ttctgaatgt catcaaagtt tgatagcttt gacaaccgtt 10620 ggattaagta tcggtattgt attttataat aatattttac tcaatagaat agaaatgttt 10680 tattcaattt taagcatcgt atttattcca attgctatag attacattag tttgaaattt 10740 aaacaaaaag atgctgtgcg actaatgctg acgataggta ttttgttaat tacacttgtg 10800 ccttactata tacaggttag cggtaattat tcaggaatat tgccttatgt tattcaacaa 10860 taaaaaataa agtttagaga ggaaataatg gaggatagaa agaaacaagc aattttgata 10920 ctatcccaca gaaatactct cgctctaaaa tcaacaatag agcttttgga ttctcaatac 10980 tttgatttct ttcttcatat agataaaaaa agtagaattc aagatttttt ttatttaaaa 11040 aaaattacaa aattctccac tattcatttt tcagaaagaa aaaatgtaca ttggggaggt 11100 ttttctatgg tagaagcaat gtttgcgcta ttagaatgtg cacgtgatac aggagaatat 11160 tcttattttc attttttatc tggagatgat atgccaatca aagataatga aatagtattt 11220 aatttttttg aaaatagtta tcctaaaaat tttattgata ttctagattt tgaaaatgtc 11280 aataaaaatt catatttcta cgaaccccct gagatgatag aggagagagt gaagtactac 11340 tatcctcata tggatattct aaacagaaaa ggaacaaatt tcatagggaa aaaactaatt 11400 tatctacaaa aattgttgaa agttaatcgc ttgaaaaata gagagataga aattttcaag 11460 ggtcatcaat ggtgtagttt gacaaatcaa tttgtagata ttttattgga taaagaggaa 11520 agaagagtag gtaagtctta tttttcatct agtttaatac cagatgaatg ttattttcaa 11580 acgtttgcta tgataaaaaa agttgaaatt tatcaacaga aaaatatgtc agcacgctta 11640 attgattgga caagagggaa accatatatt tggcgacagg atgatttttt tgaaattatg 11700 aatgataaag attcaatgtt ttctaggaag tttgatgaaa atgtagatcg taaaataatt 11760 gaagaaattt atataaaaat aagaggaaga agtactgatg aagcaaataa aatcaaagat 11820 aagagattta caaaataatt ttacctatgt ttttggaaag aaaacttttc ttggaagggg 11880 agaagcgatt atcatagatg aacctgagca tggaaatttg ggagatcaag caattgcttt 11940 tgcagaaaat caatttttag taaatcatgt atcagtacga gatgtagaac atcttataga 12000 aagcaaaact atttcagaaa taaaatctat aaaaaaaaat attggaaaaa aagaattagt 12060 tttttttcat gggggaggaa atttcgggac actttatcta aagtatgagc gcattagaag 12120 attggcagta tcaaagcttc cctttaataa aatgattcta tttcctcagt caatttcatt 12180 tgaagatagt aggtttggtc agaagcagct gaataaaagt aaaaaaatat acagtcaaaa 12240 tacaaatttt attttgactg caagagaacc aaaatcttat ggtttaatga agaaatgttt 12300 tccatataac aaagtaatct tgacaccgga tatcgtgctc tcatttaaat ttgaagtcac 12360 catttctgat acgcatattg ggaaagaaaa ggatagtgtt ataacttatg aaaatcgtca 12420 acactatctt gagataaagt gggatgaaat tgcgcagcat gaggtcgcct taactgatag 12480 attacatggt atgatttttt catatatcac aggcacacca tgtgttgttt tggctaataa 12540 taatcataaa attgaaggaa catacaaaca ttggttgaat gaagtcaact atattcgttt 12600 tattgaaaat ccgactgttg aaaatatttt agatgcaatc aatgacttaa agcaaatcga 12660 acctcactat attgatttat ctgataaatt tcaaccacta attgatgcga taaaagggta 12720 aaggtttaat gaataaatat aaaaaactac tatccaactc tcttgttttc acgataggaa 12780 acttaggcag caaactgtta gtctttttac tcgtaccgct ctacacctat gcgatgacac 12840 cgcaagagta tggtatggca gacttatatc aaacaacagc aaatctactt ttgccattaa 12900 ttacaatgaa tgtatttgat gcaactttac gttttgctat ggaaaagtca atgacaaaag 12960 agagtgtgtt aacaaattct cttgtggttt ggtgttttag cgcggtgttc acttgtttgg 13020 gcgcttgtat tatctatgcg ttgaacttga gtaataaatg gtatttagct ttacttttaa 13080 ccttcaactt atttcaaggt ggacaaagta tattaagcca gtatgctaga ggtataggaa 13140 agtcgaaaat atttgcagct ggcggagtta ttttaacctt tttgacaggc gctttaaata 13200 ttcttttttt ggtatattta ccgcttggga ttacgggcta tttaatgtcc ctggttttag 13260 cgaatgtagg tacgattcta ttttttgctg gcacactttc catttggaag gaaattagtt 13320 ttaaaataat tgataaaaaa ctgatttggc aaatgctcta ttatgcctta cctttgattc 13380 ctagttccat cctgtggtgg ttactgaatg cttctagtcg ctatttcgtt ttattctttt 13440 taggagcagg tgctaatggt cttttggcgg tcgctaccaa aattccaagt attatttcca 13500 tttttaatac gatttttcca caggcgtggc aaatttcagc catagaagaa tatgattctc 13560 atcaaaaatc aaaatattat tcggatgttt ttcactactt agcaactttt ctattgttag 13620 ggacatcagc ttttatgatt gtgcttaaac caattgtcga aaaagtcgtt tcaagtgact 13680 atgcaagttc atggcaatat gttcctttct ttatgttgtc gatgctattt tcctcgtttt 13740 ctgatttttt tgggactaat tatattgcgg ctaaacaaac aaaaggcgta tttatgacat 13800 ctatctatgg taccattgtt tgtgtcttac tccaagtggt gctgctaccc atcatcggct 13860 tggatggcgc aggtttatca gccatgcttg gatttttaac aacgttttta ttgcgtgtca 13920 aagatacgca aaaatttgtg gtgattcaga ttaagtggcg gatttttatc agtaatttat 13980 tgatcgtttt ggcacaaatt ttatgtttgt tttatctacc gagtgaattt ttgtattttg 14040 gtcttgccct attattttgt ggcatgttag tggttaatca gcgtacaatt ttatacatta 14100 tcatggcgct aaaaataaaa aataagacat ttggaatgaa atcctcataa aaatagacag 14160 gaggtgtatc tcgaatggta tcgagatata tctcctgtct atttttatga tacttttgtg 14220 ttagctcaac tcaaccgcct tttaatctcc caacaacaat aatacccaat caaacaaccc 14280 aaaaaattca agataatatc actaatggca aatgtgccca aataaaagat aaattgaacg 14340 gtttcaatta ctaaaagagt gaccaaactg acaatgacaa actgtttgaa atcagtattg 14400 atacagtaaa ggccacctaa aggaatgaag tagataatat ttagcacagc ctcttgaatc 14460 gttctgggat ccgcttttat aaagtcaaaa ggattcagtg acatcgcctg aaaatccgtt 14520 attttagtaa aaagtaccat gaataacagt aataaataca cactgaaagc aagatagaga 14580 taaataactg aaaaatattt gaggtgatac tggataccaa acaaccagat aatcagcgtt 14640 aataagagta ttaaagtcaa tgtggtatag tcaaagtggt taatcaac 14688 // |
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