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EBI DbfetchID D90243; SV 1; linear; genomic DNA; STD; PRO; 3594 BP. XX AC D90243; XX DT 24-JAN-1992 (Rel. 30, Created) DT 07-DEC-2007 (Rel. 94, Last updated, Version 8) XX DE Bacillus ohbensis CGT gene for cyclodextrin glucanotransferase, complete DE cds. XX KW . XX OS Bacillus ohbensis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3594 RX DOI; 10.1007/BF00169623 RX PUBMED; 1368710. RA Sin K.A., Nakamura A., Kobayashi K., Masaki H., Uozumi T.; RT "Cloning and sequencing of a cyclodextrin glucanotransferase gene from RT Bacillus ohbensis and its expression in Escherichia coli"; RL Appl. Microbiol. Biotechnol. 35(5):600-605(1991). XX CC These data kindly submitted in computer readable form by: CC Kyeong-A Sin CC Department of Biotechnology CC Faculty of Agriculture CC The University of Tokyo CC 1-1-1 Yayoi, Bunkyo-ku CC Tokyo 113 CC Japan XX FH Key Location/Qualifiers FH FT source 1..3594 FT /organism="Bacillus ohbensis" FT /strain="C-1400" FT /mol_type="genomic DNA" FT /db_xref="taxon:1481" FT misc_feature 1000..1041 FT /note="secondary structure" FT repeat_region 1000..1018 FT /rpt_type=INVERTED FT /note="inverted repeat A" FT repeat_region 1022..1041 FT /rpt_type=INVERTED FT /note="inverted repeat A'" FT RBS 1095..1100 FT /note="rRNA binding site" FT CDS 1108..3222 FT /codon_start=1 FT /transl_table=11 FT /gene="CGT" FT /product="cyclodextrin glucanotransferase" FT /EC_number="2.4.1.19" FT /note="CGTase precursor" FT /db_xref="GOA:P27036" FT /db_xref="InterPro:IPR002044" FT /db_xref="InterPro:IPR002909" FT /db_xref="InterPro:IPR006046" FT /db_xref="InterPro:IPR006047" FT /db_xref="InterPro:IPR006048" FT /db_xref="InterPro:IPR006589" FT /db_xref="InterPro:IPR013780" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR013783" FT /db_xref="InterPro:IPR013784" FT /db_xref="InterPro:IPR014756" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/Swiss-Prot:P27036" FT /protein_id="BAA14289.2" FT /translation="MKNLTVLLKTIPLALLLFILLSLPTAAQADVTNKVNYTRDVIYQI FT VTDRFSDGDPSNNPTGAIYSQDCSDLHKYCGGDWQGIIDKINDGYLTDLGITAIWISQP FT VENVYALHPSGYTSYHGYWARDYKRTNPFYGDFSDFDRLMDTAHSNGIKVIMDFTPNHS FT SPALETDPSYAENGAVYNDGVLIGNYSNDPNNLFHHNGGTDFSSYEDSIYRNLYDLADY FT DLNNTVMDQYLKESIKLWLDKGIDGIRVDAVKHMSEGWQTSLMSDIYAHEPVFTFGEWF FT LGSGEVDPQNHHFANESGMSLLDFQFGQTIRDVLMDGSSNWYDFNEMIASTEEDYDEVI FT DQVTFIDNHDMSRFSFEQSSNRHTDIALAVLLTSRGVPTIYYGTEQYLTGGNDPENRKP FT MSDFDRTTNSYQIISTLASLRQNNPALGYGNTSERWINSDVYIYERSFGDSVVLTAVNS FT GDTSYTINNLNTSLPQGQYTDELQQLLDGNEITVNSNGAVDSFQLSANGVSVWQITEEH FT ASPLIGHVGPMMGKHGNTVTITGEGFGDNEGSVLFDSDFSDVLSWSDTKIEVSVPDVTA FT GHYDISVVNAGDSQSPTYDKFEVLTGDQVSIRFAVNNATTSLGTNLYMVGNVNELGNWD FT PDQAIGPMFNQVMYQYPTWYYDISVPAEENLEYKFIKKDSSGNVVWESGNNHTYTTPAT FT GTDTVLVDWQ" FT sig_peptide 1108..1194 FT /gene="CGT" FT mat_peptide 1195..3219 FT /gene="CGT" FT terminator 3269..3306 FT /note="transcriptional terminator" FT repeat_region 3269..3286 FT /rpt_type=INVERTED FT /note="inverted repeat B" FT repeat_region 3289..3306 FT /rpt_type=INVERTED FT /note="inverted repeat B'" XX SQ Sequence 3594 BP; 1088 A; 703 C; 709 G; 1094 T; 0 other; gagctcgccg ccttcgtaag cgccttcatt atttaaacca acatcctccg tatcatattc 60 ccctacatca ccgccaaaaa tataggcacc gtaatttttg aggaaaggaa aagcaaaata 120 gagatcatta ggtctcatca gaaatccata ttgatcagct gatgcatccg tattttcttc 180 aagaatcgtt cttaaatctt cgatcgtctc tggtgcttct ggtacaattt ctttattgta 240 gaatattcca tatgtttcga taacagctgg aactccataa atatccgttt ctccttcgaa 300 ctcataggtg accgcatcta tagaggaact agcataattg cttaactcgt catctgatag 360 agtaagagga tcagctaatc cttgtgccac aatatttccg atctggtcat gtggctggaa 420 aaataaatcg ggaccattcc cttctggacc tgctaatgac aattcctgca attggtccat 480 catcggcttt gtctcaacct tcacgttaat tcccgtttgc tctgtataat cgttgctatt 540 ttttcaatcg cctctaattg ctcttctcta tcatttgccc agatgggtta attcttcagg 600 ttgatccgct tccccaccat ttgtatccgt ggttactgcc tcttcttccc gttcaggtgc 660 acatgccgct aagagaataa tcattaaaaa catggtaatc agtgataata atgaaaaacc 720 ttttttcatt tcatttcccc cctgaaaaaa ttatattaat ctagtaaatg ttgagctacc 780 tttacataaa ggaaagtaac gcaaacgttt gcactagatt agatacaaca atcatacatg 840 gcaattcaat tttttgcaat cgtttacagt aaagaatttt ttgaattttt atttagttac 900 aattacctat ttacaaatga aatgtctatg agataaaaat aaattaacaa ctatgaatgt 960 tcatattctc aatacgaggg caatgctttt gttttttaca aacaaccccg tcttttattc 1020 cattaaaagg cggggttatt ttattggata aggagggttg tattgcttgt tcatacgatt 1080 catttttaat gtataggggg agtatttttg aaaaatttaa cggttttatt gaaaacgatt 1140 ccattagctc tgttactttt catccttctt tctttaccaa cagcggctca ggctgacgtt 1200 acaaataagg ttaattatac aagagatgtt atttatcaaa ttgtgaccga ccgtttttct 1260 gatggagatc catccaacaa cccaacgggg gcgatttata gtcaggattg tagcgacctt 1320 cataaatatt gtgggggcga ctggcaagga attatcgaca aaatcaatga cgggtattta 1380 accgatttag gaattacagc gatttggatt tcacagcctg tagaaaatgt ctatgctctt 1440 catccgagtg gttatacctc ttatcacggg tattgggcaa gagattataa aagaacgaac 1500 cctttttatg gcgatttctc tgactttgac cggttaatgg atactgcaca tagtaatggc 1560 attaaggtaa tcatggactt tacgcccaac cattcatcac cagctcttga aaccgatcca 1620 agctatgccg agaacggagc ggtttataat gatggcgtat taataggcaa ttattctaac 1680 gaccctaaca acctctttca ccataatggt gggacagatt tttcatccta tgaagatagc 1740 atttatcgaa atctatatga tttagccgac tatgatttaa acaatactgt aatggaccaa 1800 tatttaaaag aaagtataaa gctttggtta gataaaggaa ttgatgggat tcgagtcgat 1860 gcggtcaaac atatgtccga gggctggcaa acctccttaa tgagtgatat ttatgcacac 1920 gagcctgtct ttacatttgg cgaatggttt ttaggatcag gggaagtcga cccacaaaat 1980 catcactttg ccaatgaaag tggtatgagc ttactagact ttcagtttgg tcaaacgatt 2040 agagatgtat taatggacgg tagcagcaat tggtatgact ttaatgagat gattgcaagc 2100 accgaagagg attatgacga agtgattgat caggttacgt ttattgataa tcatgacatg 2160 agccgttttt cctttgaaca atcttcaaac cgccatacag acattgcttt agctgtcctg 2220 ttaacctctc gtggagttcc gactatatat tacggtacag agcaatattt aacaggaggc 2280 aacgaccctg aaaaccgcaa gccgatgagc gattttgacc gtacgacaaa ttcttatcaa 2340 attattagta cgcttgcttc cttaagacaa aacaacccgg cattaggcta tggaaataca 2400 agcgaacgat ggattaactc cgacgtttat atttatgaac gatccttcgg agacagtgtc 2460 gtgcttactg ctgttaacag tggcgatact agttacacga ttaataactt aaatacctcg 2520 ttacctcaag gtcaatatac agatgagctt cagcagcttt tggatggaaa tgagattaca 2580 gtcaatagca atggagctgt agactcgttc cagctaagtg caaatggagt atccgtgtgg 2640 caaataaccg aagagcatgc ttctcctcta atcggccatg taggaccaat gatgggtaaa 2700 cacggaaata ctgtcactat aacgggagaa ggctttggtg acaatgaggg aagtgttctc 2760 tttgattctg atttctctga tgtcctttct tggtctgaca cgaaaataga agtaagcgtt 2820 ccggatgtaa cagctggtca ctatgatatt agcgttgtaa atgctggaga cagccaaagt 2880 ccaacgtatg ataagtttga agtattaacc ggtgaccaag ttagtatccg ttttgctgtt 2940 aataatgcga ccactagcct tggtaccaat ctgtatatgg ttggaaatgt gaatgagctt 3000 ggaaattggg accctgatca agcgattggg ccgatgttta atcaagtgat gtatcaatac 3060 ccaacctggt actatgatat aagtgttcct gccgaggaaa atcttgaata taaatttatt 3120 aaaaaggaca gcagcggaaa cgtcgtttgg gaaagtggaa ataaccatac gtatacaaca 3180 cctgcaactg gaaccgatac agtcctcgtc gactggcaat aacacaaaat acacatcaac 3240 ttaagggcta tgcattgtga tcatacacaa caaccccatt ttccaagatt ggaaataggg 3300 gttgttttat aatgaaaggg gaattttcct atgttaaaag aagcgattta tcaccgacca 3360 aaaaataatt atgcgtacgc ctattcaaag gatacgctac acattcgtct acgcaccaaa 3420 aagaatgatc tcacgcaagt cgagcttctc tatgctgatc cttacaattg gaatgaagat 3480 ggttggctct atgagcaaaa agcgatgcgt ttagaggctt ctgaccactt atttgactat 3540 tggatgattg atgttaccgt cccataccgt cgactccgct atggctttaa gctt 3594 // |
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