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EBI DbfetchID D90213; SV 1; linear; genomic DNA; STD; PRO; 5100 BP. XX AC D90213; XX DT 12-MAR-1991 (Rel. 27, Created) DT 07-DEC-2007 (Rel. 94, Last updated, Version 6) XX DE Streptococcus sobrinus gtf-I gene for glucosyltransferase, complete cds. XX KW mutan. XX OS Streptococcus sobrinus OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus. XX RN [1] RP 1-5100 RX PUBMED; 1704006. RA Abo H., Matsumura T., Kodama T., Ohta H., Fukui K., Kato K., Kagawa H.; RT "Peptide sequences for sucrose splitting and glucan binding within RT Streptococcus sobrinus glucosyltransferase (water-insoluble glucan RT synthetase)"; RL J. Bacteriol. 173(3):989-996(1991). XX CC These data kindly submitted in computer readable form by: CC Hirokazu Abo CC Department of Microbiology CC Okayama University Dental School CC 2-5-1 Shikata-cho CC Okayama 700 CC Japan XX FH Key Location/Qualifiers FH FT source 1..5100 FT /organism="Streptococcus sobrinus" FT /strain="6715" FT /mol_type="genomic DNA" FT /db_xref="taxon:1310" FT -35_signal 109..114 FT -10_signal 132..137 FT RBS 166..172 FT CDS 181..4959 FT /codon_start=1 FT /transl_table=11 FT /gene="gtf-I" FT /product="glucosyltransferase precursor" FT /db_xref="GOA:P27470" FT /db_xref="HSSP:1GVM" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P27470" FT /protein_id="BAA14241.1" FT /translation="MEKNVRFKMHKVKKRWVTLSVASATMLASALGASVASADTDTASD FT DSNQTVVTGDQTTNNQATDQTSIAATATSEQSASTDAATDQASAAEQTQGTTASTDTAA FT QTTTNANEAKWVPTENENQGFTDEMLAEAKNVATAESDSIPSDLAKMSNVKQVDGKYYY FT YDQDGNVKKNFAVSVGDKIYYFDETGAYKDTSKVDADKSSSAVSQNATIFAANNRAYST FT SAKNFEAVDNYLTADSWYRPKSILKDGKTWTESGKDDFRPLLMAWWPDTETKRNYVNYM FT NKVVGIDKTYTAETSQADLTAAAELVQARIEQKITSENNTKWLREAISAFVKTQPQWNG FT ESEKPYDDHLQNGALLFDNQTDLTPDTQSNYRLLNRTPTNQTGSLDSRFTYNPNDPLGG FT YDFLLANDVDNSNPVVQAEQLNWLHYLLNFGSIYANDADANFDSIRVDAEDNVDADQLQ FT ISSDYLKAAYGIDKNNKNANNHVSIVEAWSDNDTPYLHDDGDNLMNMDNKFRLSMLWSL FT AKPTDVRSGLNPLIHNSLVDREVDDREVETVPSYSFARAHDSEVQDIIRDIIKAEINPN FT SFGYSFTQEEIDQAFKIYNEDLKKSDKKYTHYNVPLSYTLLLTNKGSIPRVYYGDMFTD FT DGQYMANKTVNYDAIESLLKARMKYVAGGQAMQNYQIGNGEILTSVRYGKGALKQSDKG FT DATTRTSGVGVVMGNQPNFSLDGKVVALNMGAAHANQEYRALMVSTKDGVATYATDADA FT SKAGLVKRTDENGYLYFLNDDLKGVANPQVSGFLQVWVPVGAADDQDIRVAASDTASTD FT GKSLHQDAAMDSRVMFEGFSNFQSFATKEEEYTNVVIANNVDKFVSWGITDFEMAPQYV FT SSTDGQFQDSVIQNGYAFTDRYDLGMSKANKYGTADQLVKAIKALHAKGLKVMADWVPD FT QMYTFPKQEVVTVTRTDKFGKPIAGSQINHSLYVTDTKSSGDDYQAKYGGAFLDELKEK FT YPELFTKKQMSTGQAIDPSVKIKQWSAKYFNGSNILGRGADYVLSDQVSNKYFNVASDT FT LFLPSSLLGKVVESGIRYDGKGYIYNSSATGDQVKASFITEAGNLYYFGKDGYMVTGAQ FT TINGANYFFLENGTALRNTIYTDAQGNSHYYANDGKRYENENGYQQFGNDWRYFKDGNM FT AVGLTTVDGNVQYFDKDGVQAKDKIIVTRDGKVRYFDQHNGNAVTNTFIADKTGHWYYL FT GKDGVAVTGAQTVGKQKLYFEANGQQVKGDFVTSDEGKLYFYDVDSGDMWTDTFIEDKA FT GNWFYLGKDGAAVTGAQTIRGQKLYFKANGQQVKGDIVKGTDGKIRYYDAKSGEQVFNK FT TVKAADGKTYVIGNDGVAVDPSVVKGQTFKDASGALRFYNLKGQLVTGSGWYETANHDW FT VYIQSGKALTGEQTINGQHLYFKKDGHQVKGQLVTGTDGKVRYYDANSGDQAFNKSVTV FT NGKTYYFGNDGTAQTAGNPKGQTFKDGSDIRFYSMEGQLVTGSGWYSNAQGQWLYVKNG FT KVLTGLQTVGSQRVYFDENGIQAKGKAVRTSDGKIRYFDENSGSMITNQWKEVNGRYYY FT FGNDGARIYRGWN" FT sig_peptide 181..294 FT /note="glucosyltransferase signal peptide" FT mat_peptide 295..4956 FT /product="glucosyltransferase" FT terminator 4990..5016 FT /note="put. transcriptional termination sequence" FT terminator 5042..5059 FT /note="put. transcriptional termination sequence" XX SQ Sequence 5100 BP; 1575 A; 1051 C; 1111 G; 1363 T; 0 other; taagatcagt cctcaacaga aattgcctta tttaaaaaga aattaagaaa aaaacgttta 60 atataatgtt aataactaga aataaattgc aaattgtggt aaaattaatt gatggttaga 120 ataatttaac tcgtaaaaaa gttaactgtc gttatttttc aaattaggag gactccattc 180 atggagaaga atgtacgttt taagatgcat aaggtgaaaa agagatgggt aaccctctct 240 gtcgcatctg ccaccatgtt ggcatcagcc cttggtgctt cagtagctag tgcggataca 300 gacactgcta gtgatgatag caaccaaacc gtggtaactg gtgaccagac tactaacaat 360 caagccactg accagacttc tattgcagca acagctacat cagaacagtc tgcttcaact 420 gatgcagcaa cagatcaagc atcagcagca gagcaaactc aaggaacaac agctagcaca 480 gacacggcag ctcaaacaac cacaaatgct aatgaagcta agtgggttcc gactgaaaat 540 gagaaccaag gttttacaga tgagatgtta gcagaagcca agaatgtggc tactgctgaa 600 tctgattcaa ttccatcaga cttggctaaa atgtcaaatg ttaagcaggt tgacggtaaa 660 tattattact acgaccaaga tggcaatgtt aagaaaaact ttgctgtcag cgttggtgat 720 aagatttatt actttgatga aactggcgct tacaaggaca ctagcaaggt tgatgccgac 780 aagtccagtt cagctgtaag tcaaaatgca acaatatttg cagctaataa ccgtgcctac 840 agcacctcag ctaaaaattt tgaagccgtt gataactacc tgacagctga ctcttggtat 900 cgtccaaaat caatcctgaa agacggaaaa acttggacag aatctggcaa agatgacttc 960 cgcccgcttc tcatggcttg gtggcctgat accgaaacca aacgtaacta cgttaattac 1020 atgaacaagg ttgttggtat tgataagacc tataccgctg aaaccagcca agctgattta 1080 acggcagcag cagaattggt tcaagctcgt attgaacaaa aaattacaag tgaaaataac 1140 actaagtggc tccgtgaggc gatttctgcc tttgtgaaaa ctcagccgca atggaatggt 1200 gaaagcgaaa agccttacga tgatcacttg caaaatggtg ctcttctctt tgacaatcaa 1260 actgatttaa caccagatac gcaatcgaac tatcgtttgc tcaatcgcac accaacaaac 1320 caaactggtt ccttggattc tcgtttcacc tataacccaa atgacccact gggcggctat 1380 gatttccttt tagccaacga tgttgataat tccaatccag tcgtgcaagc ggaacaactc 1440 aactggctgc actacctgct caactttggc tctatctatg ccaatgatgc agatgccaat 1500 tttgactcaa tccgtgtaga tgcggaagat aatgttgatg ctgaccaact gcaaatctct 1560 agtgattacc ttaaggcagc ttacggtatc gataaaaaca acaaaaatgc aaataaccac 1620 gtttctatcg tagaagcatg gagcgacaac gatacccctt atctccatga tgatggcgac 1680 aacctcatga acatggacaa caagttccgt ttgtccatgc tttggtcttt agctaagcca 1740 accgatgttc gttctggttt gaatcctttg atccacaaca gtctggttga ccgtgaagtg 1800 gatgaccgtg aagttgaaac cgttccaagt tacagctttg ctcgggctca tgatagtgaa 1860 gttcaggata tcattcgtga tattattaag gctgagatta atccaaattc atttggttat 1920 tcattcaccc aagaagaaat tgatcaagct ttcaagattt acaacgaaga tctcaagaag 1980 tctgataaaa aatacactca ctacaatgtg ccgctttctt ataccttgct tctgactaac 2040 aagggttcga ttcctcgcgt ctattatgga gatatgttca ccgatgatgg tcaatacatg 2100 gccaacaaga ctgtgaacta cgatgctatc gaatctctgc tgaaagcccg tatgaagtac 2160 gttgctggtg gtcaggctat gcagaattac caaatcggta atggcgaaat cttgacttct 2220 gtccgttatg gtaagggtgc ccttaaacaa agcgataagg gtgatgcgac aactcgtacg 2280 tcaggtgtcg gcgttgttat gggaaaccaa cccaacttta gcttggatgg aaaggttgta 2340 gccctcaaca tgggtgctgc ccacgctaac caagaatacc gtgctcttat ggtatcaact 2400 aaagacggtg ttgcaaccta tgctacagat gctgatgcta gcaaggctgg tctggttaag 2460 cgcacagatg aaaatggtta cctctacttc ttgaacgacg atctcaaggg ggttgctaac 2520 cctcaggttt ctggtttcct tcaagtctgg gtaccagtgg gagcagcaga tgaccaagat 2580 attcgtgtag cagctagcga tacagcaagt accgatggaa aatcactcca tcaagatgct 2640 gccatggact ctcgcgtcat gtttgaaggt ttctctaact tccaatcttt tgcgacaaaa 2700 gaagaagagt ataccaatgt tgtcattgct aacaatgttg ataaatttgt ttcatgggga 2760 atcactgact ttgaaatggc tcctcagtat gtctcatcta ctgacggtca gttccaagat 2820 tctgtcattc aaaatggtta tgcctttacc gaccgttatg acttgggtat gtctaaagca 2880 aacaagtatg gtacagccga ccaattggtt aaggctatca aggctctcca tgctaagggc 2940 ctgaaggtta tggcagactg ggttccagac caaatgtaca ccttccctaa acaagaagtg 3000 gtcactgtta ctcggacaga taagtttggc aaaccaatcg caggaagcca aattaatcac 3060 agtctctacg taacagatac aaagagctct ggtgatgact atcaagctaa atacggcggt 3120 gccttccttg acgaattaaa ggaaaaatat ccagaactct tcaccaagaa gcaaatgtct 3180 actggtcagg cgattgatcc atctgttaag attaaacaat ggtctgctaa gtactttaat 3240 ggaagtaata ttcttggccg gggtgccgat tatgtcctca gcgaccaagt cagcaacaag 3300 tacttcaacg ttgccagcga tacactcttc ttaccaagca gcttactcgg caaggtcgta 3360 gagtctggta ttcgttatga tggtaagggt tatatttata actcaagtgc aactggtgac 3420 caagtcaaag caagcttcat taccgaagca ggcaatctat actacttcgg taaagacggt 3480 tatatggtga ctggcgctca aaccattaat ggtgctaact atttcttcct tgaaaatggt 3540 acggctcttc gcaacactat ttatacagat gctcaaggca atagccatta ctacgcaaat 3600 gacggtaaac gctatgaaaa tgaaaatggt taccaacaat ttggtaatga ctggcgttac 3660 ttcaaggacg gtaacatggc tgttggcttg acaactgttg atggcaatgt tcaatacttt 3720 gataaagatg gtgttcaagc taaggataag attattgtca cccgtgatgg taaggttcgt 3780 tactttgacc aacataatgg aaatgctgta accaatacct tcatcgctga caagactggt 3840 cactggtact atctaggtaa agatggtgtc gctgttaccg gtgctcaaac cgttgggaaa 3900 caaaaacttt actttgaagc aaacggtcaa caagttaagg gtgacttcgt aacttctgac 3960 gaaggtaaac tttacttcta cgatgtcgat tcaggtgaca tgtggactga taccttcatt 4020 gaagataagg caggcaattg gttctacctt ggtaaagatg gtgcagctgt gactggtgct 4080 caaactattc gtggccaaaa actttacttc aaggctaacg gccaacaagt caagggagat 4140 atcgtcaagg gtactgatgg taagatccgt tactacgacg ctaaatctgg tgaacaagtc 4200 ttcaacaaga ctgttaaggc cgctgatggc aagacctatg ttatcggaaa tgatggtgtt 4260 gcagttgatc caagcgttgt caaaggacaa accttcaagg atgcttcagg tgctcttcgt 4320 ttctataacc tcaaaggaca actggtaaca ggcagcggtt ggtatgaaac tgcaaatcac 4380 gattgggttt atatccaatc tggtaaagcc ttgactgggg aacagaccat caatggtcaa 4440 catctttact tcaagaaaga tggacatcaa gtcaaaggac aactggtaac aggaactgat 4500 ggtaaggttc gctattatga tgcaaattca ggcgaccaag ccttcaacaa gtctgtaaca 4560 gttaacggta agacttacta cttcggtaat gatggcactg ctcaaacagc gggaaaccct 4620 aagggacaaa ccttcaaaga tggttcagat atccgctttt acagcatgga aggccaatta 4680 gtgactggca gtggttggta ctcaaacgca caaggtcagt ggctttatgt caaaaatggt 4740 aaagtcttga caggcctgca aacagttggt agccaacgtg tttactttga cgaaaatggt 4800 attcaagcta aaggtaaagc agtaaggact tccgacggta agatacgcta cttcgatgaa 4860 aattcaggta gcatgattac caaccaatgg aaagaggtta acggtcgata ttattacttc 4920 ggtaatgatg gcgcacgtat ctaccgtggc tggaactaat atttgagatt ttattttctc 4980 aatacagaga agacttcctc ggactgagga agtctttttt ggtttagcgc tttaagtcca 5040 tttttcttag attagaaaag accacccgtg aaattttccc gagtggtttt gtctatttga 5100 // |
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