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EBI DbfetchID D90043; SV 1; linear; genomic DNA; STD; PRO; 2834 BP. XX AC D90043; XX DT 27-JUL-1990 (Rel. 24, Created) DT 07-DEC-2007 (Rel. 94, Last updated, Version 5) XX DE Bacillus megaterium gene for glucose dehydrogenase and ORFs. XX KW . XX OS Bacillus megaterium OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2834 RA Mitamura T., Ebora R.V., Nakai T., Makino Y., Negoro S., Urabe I., RA Okada H.; RT "Structure of isozyme genes of glucose dehydrogenase from Bacillus RT megaterium IAM1030"; RL J. Ferment. Bioeng. 70:363-369(1990). XX DR StrainInfo; 373762; IAM 1030. XX CC These data kindly submitted in computer readable form by: CC Toshihide Mitamura CC Department of Fermentation Technology CC Osaka University CC 2-1 Yamada-oka CC Suita, Osaka 565 CC Japan XX FH Key Location/Qualifiers FH FT source 1..2834 FT /organism="Bacillus megaterium" FT /strain="IAM 1030" FT /mol_type="genomic DNA" FT /db_xref="taxon:1404" FT -35_signal 89..94 FT /note="put. -35 region for ORF1" FT -10_signal 116..121 FT /note="put. -10 region for ORF1" FT RBS 167..172 FT /note="SD sequence for ORF2" FT CDS 181..867 FT /codon_start=1 FT /transl_table=11 FT /note="ORF1" FT /db_xref="GOA:P39486" FT /db_xref="InterPro:IPR001789" FT /db_xref="InterPro:IPR011006" FT /db_xref="UniProtKB/Swiss-Prot:P39486" FT /protein_id="BAA14097.1" FT /translation="MNKKAWTVLLIEDDPMVQEVNRQFIEQVEGFTVIAAASNGLEGVQ FT LIKQHQPDLTIIDMYMPSQDGLTTLQQIRANGYKTDVIAVTAASDIETVRKVLQYGAVD FT YIMKPFKFERMKQALEQYRSFQVKISQKEHITQSELDSMLFQQFEEKADLLPKGLNAVT FT LRRIQQYLSEQNHPISAEEVADGVGIARVTARRYLEFLEQENELKLSVEYGRVGRPINR FT YMLKIN" FT terminator 893..928 FT /note="termination signal" FT -35_signal 972..977 FT /note="put. -35 region for ORF2" FT -10_signal 997..1003 FT /note="put. -10 region for ORF2" FT RBS 1070..1076 FT /note="SD sequence for ORF2" FT CDS 1086..1946 FT /codon_start=1 FT /transl_table=11 FT /note="ORF2" FT /db_xref="GOA:P40419" FT /db_xref="InterPro:IPR004673" FT /db_xref="InterPro:IPR010651" FT /db_xref="UniProtKB/Swiss-Prot:P40419" FT /protein_id="BAA14098.1" FT /translation="MDIFLAVLPAIFWGSIVLFNVKLGGGPYSQTLGTTLGALIFSIGI FT YIFVHPTFTPLIFGVGVVSGLFWAVGQSNQLKSIDLIGVSKTMPISTGLQLVSTSLFGV FT IVFHEWSTKTSIILGVLALIFIIVGIVLASLQSKEEKEAEEGKGNFKKGIVILLISTVG FT YLVYVVVARLFNVDGWSALLPQAIGMVIGGVLLTFKHKPFNKYAIRNIIPGLIWAAGNM FT FLFISQPKVGVATSFSLSQMGIVISTLGGIIILGEKKTKRQLVGIIIGIILIIIAGVML FT GLAKS" FT RBS 1949..1954 FT /note="SD sequence for glucose dehydrogenase gene" FT CDS 1964..2749 FT /codon_start=1 FT /transl_table=11 FT /product="glucose dehydrogenase" FT /EC_number="1.1.1.47" FT /db_xref="GOA:P39482" FT /db_xref="InterPro:IPR002198" FT /db_xref="InterPro:IPR002347" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P39482" FT /protein_id="BAA14099.1" FT /translation="MYKDLEGKVVVITGSSTGLGKAMAIRFATEKAKVVVNYRSKEEEA FT NSVLEEIKKVGGEAIAVKGDVTVESDVINLVQSSIKEFGKLDVMINNAGMENPVSSHEM FT SLSDWNKVIDTNLTGAFLGSREAIKYFVENDIKGTVINMSSVHEKIPWPLFVHYAASKG FT GMKLMTETLALEYAPKGIRVNNIGPGAINTPINAEKFADPEQRADVESMIPMGYIGEPE FT EIAAVAAWLASSEASYVTGITLFADGGMTQYPSFQAGRG" FT terminator 2751..2783 FT /note="termination signal for glucose dehydrogenase gene" XX SQ Sequence 2834 BP; 896 A; 442 C; 644 G; 852 T; 0 other; gatcaggtag cgagaatctt tgatgaaggt ttttcaacca aagcaaagga aaatagagga 60 attggtttgc atttagtaaa acaaattgtt gaaaaaggaa acggtcagat cgaagtagag 120 tcagaattag atgttggaac gacttttatc attacattct ttttataggg ggagtgggaa 180 atgaataaaa aagcatggac cgtgcttctc atagaagacg atcctatggt acaagaagtg 240 aaccgccaat ttattgaaca agttgaaggg ttcactgtta tcgctgcagc ttcgaatggt 300 ttagaggggg tacagctcat taaacagcat cagcctgatt taacgattat tgatatgtat 360 atgcctagtc aagatggctt aaccacctta cagcaaattc gagcaaatgg ctataaaaca 420 gacgtgatag cagttacggc tgcaagtgat attgaaaccg tacgcaaagt tcttcaatat 480 ggcgctgtgg attatattat gaaaccgttc aagtttgaac gaatgaagca agcgcttgag 540 cagtatcgtt cgtttcaagt taaaataagt caaaaagaac atattactca gtctgaatta 600 gattctatgc tgtttcagca attcgaagaa aaagccgatt tgcttcccaa ggggctaaat 660 gcggttacgt taaggaggat acaacaatat ctttccgaac aaaatcatcc aatttctgct 720 gaagaagtgg cggacggcgt aggaattgcg cgtgttacag caagaaggta tttagagttt 780 ttagaacagg aaaacgagct gaaattatca gttgaatacg gcagagtggg gagacctatt 840 aatcgctata tgttaaaaat aaattaaatc atacagaaca gcttttattt ggaaaagctg 900 tttttttgcg ttagaaagta tatctttttc tctcctagaa caaattaagg tatacagttt 960 tcgctaccca aagaatattt cgtgcggtca ttaatccata aaatgtccct gaaaaggatt 1020 aatggcggaa aaattgggga atatgcactt tgacatttaa ttttaacaca ggaaggtttt 1080 gaaacatgga catattttta gccgtcttac cagccatatt ttggggaagc attgtgcttt 1140 ttaatgtgaa actaggcgga ggaccttata gtcaaacgct tggaaccaca ttgggagctt 1200 taattttctc catcggtatt tatatttttg tacaccctac gtttacacct ttaatctttg 1260 gggttggagt tgtttcgggg ctattttggg cagttggaca aagtaatcag ctgaaaagta 1320 ttgatttaat tggagtttct aaaacgatgc ctatttcaac ggggcttcag ttagtttcca 1380 cttcattatt tggagtaatt gtgtttcacg agtggtctac aaaaacttca atcattcttg 1440 gtgtgctcgc tcttatcttt attattgtag ggattgtttt agcatcactt caaagcaaag 1500 aagagaaaga ggctgaagaa ggaaaaggaa acttcaaaaa aggaattgtt attttattaa 1560 tttcaaccgt tggttattta gtttatgttg tagtagcccg tctatttaat gtagacggat 1620 ggtcggcttt attacctcaa gcaattggta tggttattgg aggagtattg ctgacgttca 1680 agcataagcc atttaataaa tatgcaattc gcaacattat cccaggtctt atttgggccg 1740 ctggtaatat gtttttattc atctcacaac ctaaagtagg cgtagcgaca agcttttcgc 1800 tttctcaaat gggaatcgtc atttcaacat taggcgggat cattatttta ggtgagaaga 1860 aaacgaagcg tcagttagtt gggattatta ttgggattat actgatcatc atagcaggag 1920 tcatgttagg gctcgccaaa agctaactag gaggttatta acaatgtata aagatttaga 1980 agggaaagta gttgtcataa caggttcatc taccggttta ggaaaagcaa tggcgattcg 2040 ttttgcgaca gaaaaagcta aagtagttgt gaattatcgt tctaaagaag aagaagctaa 2100 cagcgtttta gaagaaatta aaaaagtcgg cggagaggca attgccgtta aaggtgacgt 2160 aacagttgag tctgacgtga tcaatttagt tcaatcttct attaaagaat ttggaaagtt 2220 agacgttatg attaataacg caggaatgga aaatccggtt tcatctcatg aaatgtcttt 2280 aagcgattgg aataaagtaa ttgatacgaa cttaacggga gcatttttag gcagccgtga 2340 agcgattaaa tattttgtgg aaaatgatat taagggaaca gttattaaca tgtcgagtgt 2400 tcacgagaaa attccttggc cattatttgt tcattacgca gcaagtaaag gcggaatgaa 2460 gctcatgacc gaaacacttg cattagaata cgctccaaaa ggtattcgtg taaataacat 2520 tggaccggga gcgattaata caccgattaa cgctgagaaa tttgctgatc ctgagcagcg 2580 tgcggatgta gaaagcatga ttccaatggg atacattgga gagccggaag aaattgcagc 2640 ggttgctgca tggctagctt cttcagaggc aagttatgta acagggatta cgctctttgc 2700 tgacggcggt atgacccagt acccatcatt ccaagcagga cgcggataag aaaaaacgca 2760 ctctataata gagtgcgttt tttagtttcc ctgagctttt ttttggttct taggagctga 2820 ctggtgttga attc 2834 // |
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