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EBI DbfetchID D87672; SV 1; linear; genomic DNA; STD; PRO; 5248 BP. XX AC D87672; XX DT 15-JAN-1998 (Rel. 54, Created) DT 15-JAN-1998 (Rel. 54, Last updated, Version 1) XX DE Bacillus circulans gene for cycloinulo-oligosaccharide fructanotransferase, DE complete cds. XX KW cycloinulo-oligosaccharide fructanotransferase. XX OS Bacillus circulans OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-5248 RA Yamada K.; RT ; RL Submitted (03-SEP-1996) to the EMBL/GenBank/DDBJ databases. RL Kazunori Yamada, Mitsubishi Chemical Corporation, Yokohama Research Center, RL Molecular Medicine Laboratory; 1000, Kamoshida-cho, Aoba-ku, Yokohama, RL Kanagawa 227, Japan (E-mail:subtilis@rc.m-kagaku.co.jp, RL Tel:+81-45-963-3455, Fax:+81-45-963-3992) XX RN [2] RA Yamada K., Mitsui K., Kushibe S.; RT "Cloning of the cycloinulo-oligosaccharide fructanotransferase (CFTase) RT gene of Bacillus circulans MCI2554 and extracellular secresion of RT recombinant CFTase in Escherichia coli."; RL Unpublished. XX RN [3] RX PUBMED; 9406417. RA Kanai T., Ueki N., Kawaguchi T., Teranishi Y., Atomi H., Tomorbaatar C., RA Ueda M., Tanaka A.; RT "Recombinant thermostable cycloinulo-oligosaccharide fructanotransferase RT produced by Saccharomyces cerevisiae"; RL Appl. Environ. Microbiol. 63(12):4956-4960(1997). XX FH Key Location/Qualifiers FH FT source 1..5248 FT /organism="Bacillus circulans" FT /strain="MCI2554" FT /mol_type="genomic DNA" FT /db_xref="taxon:1397" FT CDS 468..4979 FT /codon_start=1 FT /transl_table=11 FT /product="cycloinulo-oligosaccharide fructanotransferase" FT /note="CFTase" FT /db_xref="GOA:O52973" FT /db_xref="InterPro:IPR013320" FT /db_xref="UniProtKB/TrEMBL:O52973" FT /protein_id="BAA24360.1" FT /translation="MREVKRGKKSGFAALAIWSLFFQLIAPGISAAAEPGADIEDALIS FT EAESEIPVTDAVYQIENPGFETGDLTGWSVAEGGAFGPDSVSDETVWWAERIPYDQEGA FT YHLNGWKYPESETGVLRSSTFELGGSGWISFKLGGAKDPDKAFINIVEADTGQVVARYG FT NSAFTDVGFPDPAQGMRLANMEQYKADLSGHIGKKLYIEIVDHATSDWGLIFADAFFMY FT HESEPAEGIVATDIKPDFERYQIENPSFETGDLTGWTVIEGDAFGPNSVSDETVWWAER FT IPYDQEGAYHLNGWKYPESETGVLRSSTFELGGSGWITFKLGGGKHTDQVYVSVIEAET FT GNLIARYGNSAFTDVGFPDPAQGMRLANMEQYKADLSKHIGKKLYLEIVDHGVSDWGLV FT FADAFRTFHEIVPEDGVVAENIIPAEIANRGFETGNLDGWTVEGDAFHVTDEAHAAKEG FT NFYALSSTEGQGSITSNTFTLQGAGIINFTVLDILNPEGAYVALYDASSNTVIKITGNI FT GANEQISWKVQEHYNKKLYVKVVDQSGDAGIAVDGFQARTEGTVVHLGLDEGAGKKALE FT EVRNVEHDVNYVFNDARYMAPKDPRWTPRGVKGGALLFDGYSNYIEIDADETVPVSDAL FT TIEAWVAPRSYEWGDGNKLSAIVNQSDQDKAEGFALGMYRHGTWSLQAGIGGRWIQAWV FT NDHPLEKYKWNYVAATFDKEDGMMRLYLNGEEVASQATPAGVPISPSGESLIVGKNNKP FT VELAGLFSFNMFSGLIDEVKVHNRALTNGEILAEYESAKALHGGVVPEIPNADIDEDPS FT VFDGDQHRPQYHAIPPQNWMNEAHAPIYYNGKYHLFYQHNPQGPYWHQIHWGHWVSDDM FT VHWENVRPALAPEAGSLDPDGAWSGSAAYDRNGNPVLFYTAGNDSESPNQRTGLATPAD FT LSDPYLEQWVKYPEPVTEQNGIGIHNEFRDPFVWYDEETDKWYQLVTSGLPDFSSGTAL FT VYVSDDMYNWEYKGPLYVSDRSLYPELGTVWELPVLLPLGTDSTGKKKHIFIINPHEKP FT EHVPPAKDVQRDVEVYYWIGTWDRDNFRFIPDREAPSKMDVGDGYLTAESGLVTPDGRT FT VVFSMVQNVRTPQAEYQSGWAHNLALPVALSLDEHDELRIEPIQELQSLRGNKLADISD FT KNLESANQLIKDIKGDMLEIVMEIDPGEAQKFGLKVRRSENGQEETLIYYDKSDSTFNV FT DRTKSSIDPDVRVDGIQGGYVNLDGENLKLHIFLDRSVVEAFANYKKKLTTRVYVGRYD FT SLGLQIWADGDITVKSMEVWAMNALTGEPAAPVYVPENWDNSVYKDITELPNHDFAMGD FT LTGWIAEGDAFQNIHVTDAKFFWDTIYFNPSQKIPGGYHLWGFNEQAGGDSLTGTLRSQ FT NFVLGGNGRIDFLMSGGRDIDRLYVALVRASDGKELFKETATNYEEYQRKIWDASDYIG FT EELYIKVVDQSTGGFGHLNVDDFNVPVQVMRTDRSNRSNNRS" FT sig_peptide 468..563 FT terminator 4999..5027 XX SQ Sequence 5248 BP; 1370 A; 1211 C; 1504 G; 1163 T; 0 other; gtcgaccgga cgaaaacgac gctccatccg ggagaaaagt gcggagggat tcaaggcggc 60 aagctggagc tgttaggaga gaatttgaag cttcatattt atttggaccg ctccatgatc 120 gaagcttatg caaacggatt aaaaagcttg acgacccggg cgtatccgag ccgcagcgat 180 gctttggggc ttgagctttg ggcggatggg gatacggccg ttaagtcgat ggatatttgg 240 gatatgcagt ccatttggta gcgcgagcgc ttaggcgctc cttaccgcat ggatcatgaa 300 acaatcgatc aagtgacttt acatttctgt gaattggaaa cgtttcatat ttccctctta 360 acctcttgat tattcatcct tgtaagggga gcatttcgaa atagcgaaat tcgaaatcgg 420 gcaacgatag gttggcatga ataagagacg atgaaggagg cccttcaatg agggaagtaa 480 aaagaggaaa aaaatccggg ttcgccgcgt tggcgatctg gagtctgttc tttcagctaa 540 tcgctcccgg aatctcggcc gcagccgaac ccggagccga tattgaggat gcgctcattt 600 ctgaggctga atcggaaata ccggttacgg acgcggtcta tcaaatcgaa aatccgggat 660 ttgaaaccgg ggacttgacg ggatggtccg ttgccgaagg cggagcattc ggtccggaca 720 gcgtatcgga cgaaacggtc tggtgggcgg aacgaattcc gtacgaccag gaaggcgctt 780 atcatctaaa cggctggaag tatcccgaat cggaaacagg cgtgcttcgt tccagtacgt 840 tcgagctcgg cggcagcggc tggatcagct tcaagcttgg cggcgcaaaa gatccggaca 900 aagcatttat aaatatcgtg gaagcggata cgggacaagt cgttgcgaga tacggcaaca 960 gcgccttcac cgacgtcggc tttcccgatc cggcgcaagg catgcggctt gccaatatgg 1020 agcaatataa ggccgatctt tccgggcata tcggcaaaaa attgtatata gagatcgttg 1080 accacgcgac ctcggattgg ggactgattt ttgcggacgc attcttcatg taccatgaat 1140 ccgagccggc cgaaggcata gttgcaacgg atatcaagcc ggatttcgaa cgctatcaaa 1200 tcgaaaaccc aagcttcgaa accggggatt tgacgggatg gaccgtgatc gaaggtgacg 1260 cattcggtcc gaacagcgta tcggacgaaa cggtctggtg ggcggaacga attccgtacg 1320 accaggaagg cgcttatcat ctaaacggtt ggaagtatcc cgaatcggag acaggcgtgc 1380 ttcgttccag tacgttcgag ctgggcggca gcggttggat tacgttcaag ctgggagggg 1440 gcaagcatac ggaccaagtg tatgtgagcg tcatcgaagc ggaaacggga aacttgatcg 1500 cgcgatacgg caacagcgcc ttcaccgacg tcggcttccc cgatccggcg caaggcatgc 1560 ggcttgccaa tatggagcaa tataaagcgg atctgtccaa gcatatcggc aaaaaactgt 1620 atctcgaaat cgttgaccac ggcgtatcgg actggggact ggtctttgcg gacgccttcc 1680 gcacctttca cgagatcgtg ccggaggatg gggtcgttgc cgagaatata atcccggcgg 1740 agatcgcgaa ccgcggcttt gaaaccggca atctcgacgg ctggacggtt gagggagacg 1800 cttttcacgt gaccgatgaa gctcacgccg ccaaagaagg gaacttctac gccttatcct 1860 ccacggaagg acagggctcc atcacctcca atacgttcac gcttcaagga gcgggaatca 1920 ttaacttcac cgttctggac atcctcaatc cggaaggcgc ctatgtcgcg ctttacgatg 1980 cgagcagcaa tacagtgatt aaaataaccg ggaatatcgg cgctaacgag caaatttcct 2040 ggaaggtgca ggagcactac aacaagaagc tttatgttaa ggtcgttgac caatccggtg 2100 acgccggcat tgccgttgac ggtttccaag cgcgcacgga gggtaccgtt gtccatctgg 2160 gcctcgatga aggcgccggc aagaaagcgt tggaagaagt acgaaatgtt gagcatgatg 2220 tcaactatgt ttttaacgat gcaagataca tggcgccgaa ggatccgagg tggaccccgc 2280 gcggagtaaa aggcggagcc ttgttgttcg acgggtattc gaattatatc gagatcgatg 2340 cagacgagac cgttccggta agcgacgcct taacgatcga agcatgggtt gcgccgcgca 2400 gctatgaatg gggagacggc aataagctgt ctgcaatcgt gaaccaatcc gatcaagata 2460 aagcggaagg cttcgccctc ggcatgtacc ggcacggtac atggtcgttg caggccggca 2520 ttggcggacg gtggattcag gcttgggtaa acgatcatcc gttggaaaaa tacaagtgga 2580 attatgtggc tgctactttc gataaagaag acggaatgat gagattatat ttaaacggcg 2640 aggaagtggc ttcccaagcg acgcctgccg gcgtgccgat ctcgccgtcg ggagaaagct 2700 tgattgtcgg taaaaataat aagcctgtag agctcgcagg gttgttctcc ttcaatatgt 2760 tcagcggact tatagatgaa gtgaaggtgc ataaccgagc ccttacaaac ggggagatac 2820 ttgccgaata tgagagcgcg aaggcgcttc atggcggtgt cgttccggaa attccgaacg 2880 cggacatcga tgaagaccct agcgtgttcg acggagacca gcaccgcccc caatatcacg 2940 cgatacctcc gcagaactgg atgaatgaag cccatgcgcc gatttattat aacggcaaat 3000 accatttatt ttatcaacat aacccgcaag gaccctattg gcatcaaatc cattgggggc 3060 attgggtgag cgacgatatg gtgcattggg aaaatgtaag gcctgctctt gcgcccgaag 3120 cgggctcgct tgatccggac ggcgcatggt cggggagcgc agcctatgac cgcaacggca 3180 atcccgttct gttctatacc gccggcaacg actccgagtc gccgaaccaa agaacggggc 3240 ttgcaacgcc ggccgatttg tccgatcctt atttggagca atgggtcaaa tatccggaac 3300 cggtaacgga gcagaacggg atcggcatcc acaacgagtt tcgcgatccg ttcgtatggt 3360 acgacgaaga gacggataag tggtatcagt tagtgacttc aggccttccg gatttcagca 3420 gcggcacggc tttggtgtat gtatccgacg atatgtacaa ttgggagtat aagggccctt 3480 tatacgtgag tgacagaagt ctttatccgg agttgggcac ggtttgggaa ctgccggtat 3540 tattgccttt aggcacggat agtacgggga agaaaaaaca tatttttatc atcaatcctc 3600 acgaaaagcc ggagcatgtt cccccggcga aagatgtgca aagagatgtt gaggtttatt 3660 actggatcgg cacttgggac cgggataact tcaggtttat tcccgatcgg gaggcgcctt 3720 ccaaaatgga cgtaggcgac ggctatttaa ctgctgagag cggtctggtc acgcctgacg 3780 gacgaactgt cgttttctcc atggtgcaaa acgtaaggac gccgcaagcc gaatatcaat 3840 ccggatgggc gcataatttg gctttgccgg ttgccttaag cctggatgag catgacgaat 3900 tgcgtatcga gccgattcaa gaattgcaga gtctccgggg aaataagctg gcggatattt 3960 cggacaaaaa tttggagagc gcaaatcaac taatcaagga tatcaaaggc gatatgctgg 4020 agattgtgat ggaaatcgat ccgggcgaag cgcagaaatt cggcctcaag gtgaggcgtt 4080 ccgagaacgg ccaggaggag acgctcatct actatgacaa gtcggatagt acctttaacg 4140 tggaccgtac caaaagcagc atcgatccgg acgtccgggt ggacggcatt caaggcggat 4200 acgtcaacct tgacggagag aacttgaagc ttcatatttt cctcgaccgc tcggtcgtcg 4260 aagcgtttgc caattacaag aaaaagctga caacccgcgt ctacgtaggc agatacgact 4320 ccttaggctt gcagatctgg gctgacggcg atattacggt caagtcgatg gaagtatggg 4380 cgatgaatgc gttaacgggc gaaccggctg ctccggttta tgtgcccgaa aattgggaca 4440 attccgtgta caaggatatt acggaactgc ctaaccatga ttttgccatg ggcgacttaa 4500 cagggtggat tgcggaagga gacgccttcc agaatattca tgtgaccgac gccaagtttt 4560 tctgggacac catttatttc aacccgtcgc aaaaaatacc tggcggctat catctgtggg 4620 gattcaatga gcaagccggc ggcgacagtt taacgggaac gctaagatcg caaaatttcg 4680 tccttggcgg gaacggcagg atcgatttcc ttatgagcgg cggccgcgat attgaccgtc 4740 tgtatgttgc gctcgttcgg gcgtcggacg gcaaagagtt atttaaagag acagcgacca 4800 attatgaaga atatcaacgg aaaatttggg acgcttcgga ttatatcggc gaggagcttt 4860 acattaaggt agtggaccaa tccacaggcg gttttggaca tcttaacgtc gatgatttca 4920 atgtgccggt acaagtgatg agaacagatc gttccaaccg ttcgaacaat cgctcataat 4980 tccctcggag ccatacgtaa aaggaaccgc cgaccggcgg ttcctttctt tctatttcac 5040 caattgacat atcgtaaatt atcgatataa ttacaggtat ggatcctatt gaagtgttta 5100 aagcgttgtc gaacgagtcg agactgcaga tcttgcaatg gcttaaggag ccggaaaaac 5160 attttgtccc ccacgaaggg attgatatga gaaaaatcgg ggtatgcgtc agtcaagtga 5220 ccgataagct gaacatgacg cagtcgac 5248 // |
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