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EBI DbfetchID D85236; SV 1; linear; genomic DNA; STD; PRO; 5251 BP. XX AC D85236; XX DT 31-MAY-1996 (Rel. 47, Created) DT 15-SEP-2007 (Rel. 93, Last updated, Version 8) XX DE Bacillus sp. DNA for endo-1,4-beta-glucanase, complete cds. XX KW endo-1,4-beta-glucanase. XX OS Bacillus sp. OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-5251 RA Imada K.; RT ; RL Submitted (13-MAY-1996) to the EMBL/GenBank/DDBJ databases. RL Kiyohisa Imada, Miyazaki University, Department of Materials Science; RL Gakuen Kibanadai Nishi 1-1, Miyazaki, Miyazaki 889-21, Japan RL (Tel:0985-58-2811, Fax:0985-58-2876) XX RN [2] RP 1748-4558 RA Miyatake M., Imada K.; RT "A gene encoding endo-1,4-beta-glucanase from Bacillus sp. 22-28"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..5251 FT /organism="Bacillus sp." FT /strain="22-28" FT /mol_type="genomic DNA" FT /clone="pMK5 and pM3" FT /db_xref="taxon:1409" FT -35_signal 1813..1818 FT -10_signal 1836..1842 FT RBS 1837..1843 FT CDS 1847..4198 FT /codon_start=1 FT /transl_table=11 FT /product="endo-1,4-beta-glucanase" FT /db_xref="GOA:Q45554" FT /db_xref="HSSP:1G0C" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q45554" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="BAA12744.1" FT /translation="MMLRKKTKQLISSTLILVLLLSLFPTALAAEGNTREDNFDHLLGN FT ENVKRPSEAGALQLKEVDGQMTLVDQHGEKIQLRGMSTHGLQWFPEILNDNAYKALSND FT WDSNMIRLAMYVGENGYATNPELIKQRVIDGIELAIENDMYVIVDWHVHAPGDPRDPVY FT AGAEDFFRDIAALYPNNPHIIYELANEPSSNNNGGAGIPNNEEGWKAVKEYADPIVEML FT RDSGNADDNIIIVGSPNWSQRPDLAADNPINDHHTMYTVHFYTGSHAASTESYPPETPN FT SERGNVMSNTRYALENGVAVFATEWGTSQANGDGGPYFDEADVWIEFLNENNISWANWS FT LTNKNEVSGAFTPFELGKSNATSLDPGPDQVWAPEELSLSGEYVRARIKGAKYEPIDRT FT RYTKVLWDFNDGTKQGFGVNSDSPNKEAIEVENENGTLRISGLNVSNDLSDGNFWANVR FT LSANGWGKSVDILGAEKLTMDVIVDEPTTVAIAAIPQSTKHGWANPERSVKVTEADFVK FT QDDGKYKALLTITGDDAPNLKNIGKDDENNNMNNIILFVGTEAADVIYLDNIKVTGKIV FT EIPVVHSPKGDAALPSNFEYGTRQGWDWAGESGVKTALTIEEANGSQALSWEFGYPEVK FT PSDNWASAPRLDFHKDNLVRGENDYVAFDFYIDPARATEGAMNINLVFQPPANGYWVQA FT PKTFTINFEELEEANQVNGLYHYEVKINVRDIANIQDDTVLRNMILIFADVQSDFAGRV FT FVDNVRFEASLQSRLSQLSQLTQHRLSLSR" FT sig_peptide 1847..1936 FT repeat_region 4433..4448 FT /rpt_type=INVERTED FT repeat_region 4453..4468 FT /rpt_type=INVERTED XX SQ Sequence 5251 BP; 1745 A; 798 C; 1205 G; 1503 T; 0 other; aagctttctt gattgtgacg ttcgctgccc aacattttag tcttggacaa actatgttta 60 ttgggctctg tctaattatc ggcaacttga aatctttatt tttaaatttc agtggtggta 120 aaggtgtcgc aacaagttta ggtatttttc tagcaaccga accgattgct ttattatttc 180 taggaatctt atggatctta ggcttattat ttaagaaata cattttatgg tcaggcattg 240 gtgcgatttt tttaattcct tatcaatatt ctggatacgg cgaaggtttc gctgtagtct 300 ctgctttttt tattatttta tttatgctct taaaacataa tgaaaatata aaatgggctc 360 gagtggagca agatgcttct tagaggtatt tgaatgtagt catgactgat tggaaacaat 420 agataatggt aaactgtaag aagaggctgg gacataacta aagtgtttta acgagcatag 480 gaacgatgca gaacttttcc ggagcgactg cacgctgtaa ccatcttttc gttcgaattc 540 tcgttattaa atactctttt gtccaagcct cttcttactt aatcttacat aaggtaaagg 600 aagagaaaat gaacaattat aagctcgtta ttcaatatga tggtggtcgg tacaagggct 660 ggcagcgttt agggaatggt gaaaatacga tacaaggtaa gattgaatcg gtcttatcag 720 agatggttgg aagcaagatt gaaataattg gttctagccg aacggatgcg ggcgtacatg 780 ccttagctca agtagcaaat gttaagttaa gtgataattt tacagttaaa gaggttaaat 840 gattatctga atcgttattt acctcatgac ataagtgtga ctgaggttac gcttgttcca 900 gatcgtttcc attcaaggta taacgctaaa gataaaactt atctatataa aattggaatg 960 aggaatatac ccatccgttt atgcgcaagt atagcacgca tgttgagaag aaattaaaca 1020 ttgagcaaat gaaacaagca agtcagtttt tcgttggtga acatgatttt acggcttttt 1080 ccaatgcaaa gtctaaaaaa aagacaaaca caagaacaat tcactctatt atttttgatg 1140 aaagagatgg gttcattgac attagagtta gtggcgacgg ctttctttat aatatggtta 1200 gaaaaatggt tggcacttta atagaggttg gacttggtga caagcaagct gaccaggtcc 1260 aagcgatatt agaatcgaaa gatagaagcc aagccggttt gccgacgcaa caggcttgta 1320 cttagaggaa atttcttttt aagttgttag agataaactc agaccttgtt ggaattaaaa 1380 cgaggtctga gtttttttaa tcagacgata gcatgaaacg caactgaata caaaaagtat 1440 gaggaatttg aactacagaa gatctctttt tataattatt aatacccgga acgaaaatac 1500 tatttcgaaa gcggtttaca caaaaaaccg gaggttatgg cgtttttaga taattggaag 1560 aaaaaaacaa ctctagtaat ctaaattgaa catgctataa tgagtttgtt agcagcaata 1620 tcggtcttat ttacttacta ataatgtaag cgtttaacct aagagtagac gcttatatcc 1680 gaaggaggta gattgagtca agtaggaata atgatgatgc agtgtacgag ctttaaagaa 1740 tcaggagagc agttcggtta ctcccgtatt tttttggaat gttttcgaaa gcactttcgg 1800 tattttagtt atttgactca attaagaacg ataattagga ggtaatatga tgttaagaaa 1860 gaaaacaaag cagttgattt cttccactct tattttagtt ttacttctat ctttatttcc 1920 aacagctctt gcagcagaag gaaatacgcg cgaagacaat tttgatcatt tattaggtaa 1980 tgaaaatgtt aaacgccctt cagaggccgg tgcgttacaa ctaaaagaag ttgatggaca 2040 aatgacattg gtagatcaac atggagaaaa gattcaatta cgcgggatga gtactcatgg 2100 attacaatgg tttcctgaga tcttaaatga taacgcatac aaagctcttt ctaacgattg 2160 ggattccaat atgattcgtc ttgctatgta tgtaggtgaa aatgggtacg ctaccaatcc 2220 tgagttaatc aaacaaagag tgattgatgg aattgagtta gcgattgaaa atgacatgta 2280 tgttattgtt gactggcatg ttcatgcgcc aggtgatcct agggatcctg tttatgcagg 2340 tgctgaagat ttctttagag atattgcagc attgtatcct aataatccac acattattta 2400 tgagttagcg aatgagccga gtagtaataa taatggtgga gcagggattc cgaataacga 2460 agaaggttgg aaagcggtaa aagaatatgc tgatccaatt gtagaaatgt tacgcgatag 2520 tgggaacgca gatgacaaca tcatcattgt gggtagtcca aactggagtc agcgtccgga 2580 cttagcagct gataatccaa ttaatgatca ccatacgatg tatactgttc acttctacac 2640 tggttcacat gctgcttcaa ctgagagcta tccgcctgaa actcctaact ctgaaagagg 2700 aaacgtaatg agtaacactc gttatgcgtt agaaaacgga gtagcggtat ttgcgacaga 2760 gtggggaaca agtcaagcaa atggagatgg tggtccttat tttgatgaag cggatgtatg 2820 gattgagttt ttaaatgaaa acaacattag ttgggctaac tggtctttaa cgaataaaaa 2880 tgaagtgtct ggtgcattta caccatttga attaggaaaa tcaaatgcaa caagtcttga 2940 cccaggtcca gaccaggtat gggcaccaga agagttaagt ctttctggag aatatgtacg 3000 tgctcgtatt aaaggtgcga aatatgagcc gattgaccgt actagatata caaaagttct 3060 atgggatttt aatgatggaa cgaagcaagg gtttggagtg aactcagatt ctccgaataa 3120 agaggctatt gaggttgaga atgaaaatgg cactttgaga atctcaggtt taaatgtaag 3180 taatgatctt tctgatggca acttctgggc taatgttcgt ctttctgcca atggttgggg 3240 gaagagtgtc gatattttag gtgctgaaaa actaactatg gatgttattg tggatgaacc 3300 aacgacagta gcgattgctg caattccaca aagcacaaag catggttggg caaatccaga 3360 acgttcggta aaagtgacag aagctgactt tgttaagcaa gatgacggga aatataaagc 3420 ccttttaacg attacagggg atgatgctcc gaatctaaag aacattggaa aagatgatga 3480 aaataacaac atgaacaaca ttattctttt cgtaggtact gaagcagctg acgtgattta 3540 tctggataac attaaagtaa ctggtaaaat tgttgaaatt ccagtagttc actctccaaa 3600 aggcgatgct gctcttcctt ctaattttga atacggtaca cgtcaaggtt gggactgggc 3660 tggagagtct ggagtcaaaa cggccttaac aattgaagaa gcaaacgggt cgcaagcttt 3720 atcatgggaa tttgggtatc cagaagtaaa acctagtgat aactgggctt ctgctccacg 3780 tttagatttc cacaaagata acctagttcg tggtgaaaat gattatgtag cgtttgactt 3840 ctacattgat ccagctcgtg caactgaggg agcgatgaat attaacttag tattccagcc 3900 acctgctaat ggatactggg tccaagcgcc aaaaacattt acaattaact ttgaagagct 3960 tgaagaagca aatcaagtaa atggtttata ccattatgaa gtgaaaatta acgtaagaga 4020 cattgccaac attcaagatg atacggtcct acgtaatatg atactcattt ttgcagatgt 4080 acaaagtgat tttgcgggaa gagtatttgt ggataacgtt cgttttgagg cttcactaca 4140 gagccggttg agccagttga gccagttgac ccagcaccgg ttgagcctga gccggtagat 4200 cctggtgaag aaactcctcc tgtagatgag aaggaagcgg cgaaagaaga aagagaagct 4260 gcaaaagctg aaagagaagc agctagagaa gcagccaaag aggaaagaga agaagcaaga 4320 gaggctgcaa aagaagaaag agaagcagca aaggctgaaa gagaagcggc tagagaagcg 4380 taaagctgaa agagaagcaa agaaagaagc aaagaaaaaa taagagaatc ttgtaagaac 4440 tcattggctt aggctaatga gttcttactt tttaatcgag acaaatgaat aattatagcg 4500 tgaactataa agaaataaga tatatttacg ataatagtaa tgtatactaa gtttctgatt 4560 ggaactctag cacgaagatg ttagtagttt aagaggagga cgtgtaatga caactgagat 4620 acttaataga agaaagacag atcaagaaat actgcaagct ttggtagata gtgctcctta 4680 ttatcaagcc ggatctaaag atgatttagt catctccatc tgtgatagag agaagtggct 4740 gttgcaatta aatcatccta aattatcatt agatgtcaaa gcgggagacc ctgtgtctaa 4800 ggacgatcca gtgattcaag cagcattgaa tggacaagaa ggagcgggga ggcctccagt 4860 tgagttatac ggcgttcact tttttgggaa gactacgcct atttttaacg accaaggtaa 4920 ggtaatcggg gcaatgggca tgcttatggg attgaaacga ttttagaaat cgaaaaaagt 4980 attgatcagt taaatgacat tgctgttgaa gtgagagaag tcttgagtgg aattgttgag 5040 ttagctgata agcttaagca aacgaatagc ctactgtatg aggcttctaa taaagcgaga 5100 gagaactctg aagaaataca aaagatattg gatattatta atgatatttc aagacaatct 5160 aatattcttg gtttgaatgc taatattgaa gcgtcacgag ccggaaaagc agggcttggt 5220 tttaatgtgg tagcaaatga aattcgaatt c 5251 // |
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