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EBI DbfetchID D83793; SV 1; linear; genomic DNA; STD; PRO; 2179 BP. XX AC D83793; XX DT 10-JUL-1997 (Rel. 52, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Pyrococcus sp. DNA for alpha-amylase, complete cds. XX KW alpha-amylase; ApkA. XX OS Pyrococcus sp. OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Pyrococcus. XX RN [1] RP 1-2179 RA Tachibana Y., Mendez L., Takagi M., Imanaka T.; RT ; RL Submitted (05-MAR-1996) to the EMBL/GenBank/DDBJ databases. RL Yoshihisa Tachibana, Osaka University, Faculty of Engineering, RL Biotechnology; 2-1, Yamadaoka, Suita, Osaka 565, Japan (Tel:06-879-7442, RL Fax:06-879-7448) XX RN [2] RA Tachibana Y., Mendez L., Fujiwara S., Takagi M., Imanaka T.; RT "Cloning and expression of the alpha-amylase gene from the RT hyperthermophilic archaeon Pyrococcus sp. KOD1, and characterization of the RT enzyme"; RL J. Ferment. Bioeng. 82:224-232(1996). XX FH Key Location/Qualifiers FH FT source 1..2179 FT /organism="Pyrococcus sp." FT /strain="KOD1" FT /mol_type="genomic DNA" FT /db_xref="taxon:33866" FT CDS 452..1837 FT /codon_start=1 FT /transl_table=11 FT /gene="ApkA" FT /product="alpha-amylase" FT /db_xref="GOA:O33476" FT /db_xref="HSSP:1MXG" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:O33476" FT /protein_id="BAA21130.1" FT /translation="MKKFVALLITMFFVVSMAAVAQPASAAKYSELEEGGVIMQAFYWD FT VPAGGIWWDTIRSKIPEWYEAGISAIWIPPASKGMGGAYSMGYDPYDFFDLGEYNQKGT FT VETRFGSKQELINMINTAHAYGIKVIADIVINHRAGGDLEWNPFVGDYTWTDFSKVASG FT KYTANYLDFHPNEVKCCDEGTFGGFPDIAHEKEWDQHWLWASDESYAAYLRSIGVDAWR FT FDYVKGYGAWVVKDWLNWWGGWAVGEYWDTNVDALLNWAYSSGAKVFDFPLYYKMDEAF FT DNTNIPALVDALQNGGTVVSRDPFKAVTFVANHDTDIIWNKYPAYAFILTYEGQPVIFY FT RDYEEWLNKDKLNNLIWIHDHLAGGSTSIVYYDSDELIFVRNGDSKRPGLITYINLGSS FT KVGRWVYVPKFAGACIHEYTGNLGGWVDKYVESSGWVYLEAPAYDPASGQYGYTVWSYC FT GVG" FT sig_peptide 452..529 FT /product="alpha-amylase signal peptide" FT mat_peptide 530..1834 FT /product="alpha-amylase" XX SQ Sequence 2179 BP; 548 A; 589 C; 552 G; 490 T; 0 other; ctgcaggtcg aggctgaaag aatagttatg atactcgaaa agagtggctt ccagataaag 60 gacagggaag ccctcaaggc ccttataaag gaagtcctca atacaatcga actccgctct 120 cagccagtca taaagagaat ttcagatgcc aacattgaac tgacggagat agagctgttc 180 cacctcctca acatgctcgt cttcttccag agctgcgagc tctgcgatca cgcgaaaaga 240 gttaaagacc tcatctcgtt ctgatgcatc cattcttgtt aatttctatt ttggactttc 300 aagttgttgg caactccaac gtaacataaa gacacaaaac caatattgag cattgtgcat 360 agtaatgttc tccaatgttc ttaaatggca aaacttaaat atttcaccag cagtgataca 420 tagagacacc gctgaacaca gaggtggttc catgaagaag tttgtcgccc tgctcataac 480 catgtttttc gtagtgagca tggctgccgt tgcacagcca gctagcgccg caaagtattc 540 cgaactcgaa gaaggcggcg ttataatgca ggccttctac tgggacgttc cagcgggagg 600 aatctggtgg gatacaatca gaagcaagat accggagtgg tacgaggctg gaatctccgc 660 catctggatt ccgccagcca gcaagggcat gggaggagct tattcaatgg gctacgaccc 720 atacgacttc ttcgacctcg gcgagtacaa ccagaaggga acagttgaaa ctcgctttgg 780 ctcaaagcag gagcttatca acatgataaa caccgcccat gcctacggca taaaggtcat 840 agctgatatc gtcataaacc accgcgcggg cggagacctc gagtggaacc cgttcgttgg 900 ggactacacc tggacggact tctcgaaggt ggcctcgggc aaatataccg ccaactacct 960 cgacttccac cccaacgagg tcaagtgctg tgacgagggc acatttggag gtttcccaga 1020 catagcccac gagaaggagt gggaccagca ctggctctgg gcgagcgacg agagctacgc 1080 cgcctacctc aggagcatcg gcgttgatgc ctggcgtttc gactacgtaa agggctacgg 1140 agcgtgggtc gtcaaggact ggctcaactg gtggggcggc tgggccgtcg gtgagtactg 1200 ggacacgaac gttgatgcac tcctcaattg ggcatactcg agcggcgcca aggtcttcga 1260 cttcccgctc tactacaaaa tggacgaggc ctttgacaac accaacatcc cggccttggt 1320 tgatgccctc cagaacgggg gaaccgtcgt ctctcgcgac ccgttcaagg ccgtaacctt 1380 tgtagcaaac cacgacaccg atataatctg gaacaagtac cctgcttatg ctttcatcct 1440 cacctacgag ggccagcccg tcatattcta ccgcgactac gaggagtggc tcaacaagga 1500 caagcttaac aacctaatct ggatacacga ccacctcgcg ggtggaagca cgagcatagt 1560 ctactacgac agcgacgagc tgatcttcgt gaggaacggc gactccaaga ggccgggact 1620 gataacgtac atcaacctcg gctctagcaa ggtcggaagg tgggtgtacg tgccgaagtt 1680 cgcgggcgcg tgcatccacg agtacaccgg caacctcgga ggctgggtag acaagtacgt 1740 cgagtcgagc ggctgggtct atctcgaagc tccagcttac gaccccgcca gcgggcagta 1800 cggctacacc gtctggagct actgcggggt tggatgatag cccccctttt cattcctttt 1860 ggatttcata gacctcatcc gttagataat ttcctgtaac tctctcgacc ggaatctcaa 1920 ccgtcccatt tcctgtttca attcttacca gcacatcgta gagcctcccc ctcacttcga 1980 cggtctcgtt cacgcaggtg gtctcattcg ctgtcctgaa cttgaagatt agtattgcat 2040 tcctctctag gctgtaacct atgtaaatgg cattgagaca gccggtcaag gttatgcgtc 2100 tcgttaaggc atcatcaaga gttttgtagc agttctctct cctgaaaaca aaagtgtcat 2160 tctggctctc cccctgcag 2179 // |
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