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EBI DbfetchID D83791; SV 1; linear; genomic DNA; STD; PRO; 1380 BP. XX AC D83791; XX DT 23-JAN-1997 (Rel. 50, Created) DT 15-SEP-2007 (Rel. 93, Last updated, Version 8) XX DE Bacillus subtilis DNA for pectin lyase, complete cds. XX KW pectin lyase; ppr; protopectinase. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-1380 RA Sakamoto T., Sakai T.; RT ; RL Submitted (05-MAR-1996) to the EMBL/GenBank/DDBJ databases. RL Tatsuji Sakamoto, Osaka Prefecture University, College of Agriculture; 1-1 RL Gakuen-cho, Sakai, Osaka 593, Japan RL (E-mail:sakamoto@biochem.osakafu-u.ac.jp, Tel:81-722-52-1161, RL Fax:81-722-52-0341) XX RN [2] RX DOI; 10.1016/S0014-5793(96)01257-4 RX PUBMED; 8977121. RA Sakamoto T., Kawasaki H., Sakai T.; RT "Molecular cloning and nucleotide sequence of the gene encoding RT phosphate-inducible pectin lyase of Bacillus subtilis"; RL FEBS Lett. 398(2-3):269-273(1996). XX DR StrainInfo; 51936; IFO 3134. XX FH Key Location/Qualifiers FH FT source 1..1380 FT /organism="Bacillus subtilis" FT /strain="IFO 3134" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT -35_signal 48..53 FT -10_signal 74..79 FT repeat_region 114..173 FT repeat_region 114..126 FT repeat_region 160..173 FT /rpt_type=INVERTED FT RBS 165..173 FT /note="putative" FT CDS 182..1219 FT /codon_start=1 FT /transl_table=11 FT /gene="ppr" FT /product="pectin lyase" FT /EC_number="4.2.2.10" FT /db_xref="GOA:P94449" FT /db_xref="InterPro:IPR002022" FT /db_xref="InterPro:IPR011050" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/Swiss-Prot:P94449" FT /protein_id="BAA12119.1" FT /translation="MKRFCLWFAVFSLLLVLLPGKAFGAVDFPNTSTNGLLGFAGNAKN FT EKGISKASTTGGKNGQIVYIQSVNDLKTHLGGSTPKILVLQNDISASSKTTVTIGSNKT FT LVGSYAKKTLKNIYLTTSSASGNVIFQNLTFEHSPQINGNNDIQLYLDSGINYWIDHVT FT FSGHSYSASGSDLDKLLYVGKSADYITISNSKFANHKYGLILGYPDDSQHQYDGYPHMT FT IANNYFENLYVRGPGLMRYGYFHVKNNYSNNFNQAITIATKAKIYSEYNYFGKGSEKGG FT ILDDKGTGYFKDTGSYPSLNKQTSPLTSWNPGSNYSYRVQTPQYTKDFVTKYAGSQSTT FT LVFGY" FT repeat_region 1221..1260 FT repeat_region 1221..1234 FT repeat_region 1247..1260 XX SQ Sequence 1380 BP; 451 A; 277 C; 242 G; 410 T; 0 other; ctgcagtcaa aggtgcatca atcctttctt ctgttttaat tgttagtgtg acttttagcg 60 caatctgtat ttttaaaata acacggcatt aattaggaag atagaaccta tcattccttt 120 ccatttgctg taataataat cttcacgagg aaatgattaa aattgaaagg aagcatatga 180 tatgaaacga ttttgtttat ggttcgccgt gttcagtttg ttattggttc tcttgcctgg 240 taaagcgttt ggagccgtcg attttccaaa tacctcaaca aatggccttt taggatttgc 300 cggaaatgct aaaaatgaaa aaggcatttc taaagcgagt acaacgggtg gaaaaaacgg 360 ccagattgtt tacattcaaa gcgtaaatga tttaaaaacc catctaggag gatcaactcc 420 gaaaatttta gtgcttcaaa atgatatttc cgcatcttcg aaaacaacag ttaccattgg 480 ctcaaataaa acactcgtag gctcttacgc gaaaaaaacg ttaaaaaata tttatttaac 540 cacttcatct gcctctggga acgtcatttt tcaaaatctg acttttgaac acagtccgca 600 gattaacgga aataatgata tccagcttta tttagattcc ggcatcaact actggattga 660 ccacgtcaca ttctccggcc acagctatag tgcaagcggt agtgatttgg acaaactcct 720 gtatgtcgga aaatcagcag attatatcac catcagcaac tcaaaattcg cgaatcataa 780 atacggtttg attttaggat acccagatga ttcacagcat caatatgacg gctatcccca 840 catgacaatc gccaacaatt attttgagaa cttgtatgta agaggcccgg gtctcatgag 900 atacggatat tttcatgtta aaaacaatta cagcaacaac tttaaccaag cgattacgat 960 tgccacaaaa gcaaaaatat attctgaata taattacttt ggaaaaggca gtgaaaaagg 1020 cggaatcctt gatgacaagg gtactggtta tttcaaggat acaggcagct atccttctct 1080 aaataagcaa acctctccgc taacttcctg gaatcccgga tctaattaca gctaccgtgt 1140 ccaaacgccg cagtatacaa aagattttgt cacgaaatat gcgggatcac aaagtacaac 1200 tctggtattt ggctactgac acaaaagagg cttgcgtaaa aaacgccaag cctcttttgt 1260 acttataggc tattttaagc gatcttgtta cactccatca aatctaggat tggtcaaact 1320 attcttacca aaagcgtatt ttctttttca caataacttc ttaatcggct ttcttattat 1380 // |
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