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EBI DbfetchID D83704; SV 1; linear; genomic DNA; STD; PRO; 6465 BP. XX AC D83704; XX DT 08-NOV-1996 (Rel. 49, Created) DT 07-NOV-2007 (Rel. 93, Last updated, Version 5) XX DE Clostridium thermocellum celJ, celK genes for endoglucanase J, DE endoglucanase K, complete and partial cds. XX KW . XX OS Clostridium thermocellum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-6465 RA Sakka K.; RT ; RL Submitted (25-FEB-1996) to the EMBL/GenBank/DDBJ databases. RL Kazuo Sakka, Mie University, Laboratory of Applied Microbiology; 1515 RL Kamihamacho, Tsu, Mie 514-8507, Japan (E-mail:sakka@bio.mie-u.ac.jp, RL Tel:81-592-31-9621, Fax:81-592-31-9634) XX RN [2] RX PUBMED; 8824619. RA Ahsan M.M., Kimura T., Karita S., Sakka K., Ohmiya K.; RT "Cloning, DNA sequencing, and expression of the gene encoding Clostridium RT thermocellum cellulase CelJ, the largest catalytic component of the RT cellulosome"; RL J. Bacteriol. 178(19):5732-5740(1996). XX FH Key Location/Qualifiers FH FT source 1..6465 FT /organism="Clostridium thermocellum" FT /strain="F1" FT /mol_type="genomic DNA" FT /db_xref="taxon:1515" FT CDS 595..5400 FT /transl_table=11 FT /gene="celJ" FT /product="endoglucanase J" FT /db_xref="GOA:P71140" FT /db_xref="InterPro:IPR000601" FT /db_xref="InterPro:IPR001701" FT /db_xref="InterPro:IPR004197" FT /db_xref="InterPro:IPR008928" FT /db_xref="InterPro:IPR008979" FT /db_xref="InterPro:IPR012341" FT /db_xref="InterPro:IPR014756" FT /db_xref="InterPro:IPR016134" FT /db_xref="InterPro:IPR017853" FT /db_xref="InterPro:IPR018242" FT /db_xref="PDB:1WMX" FT /db_xref="PDB:2C24" FT /db_xref="PDB:2C4X" FT /db_xref="PDB:2E0P" FT /db_xref="PDB:2E4T" FT /db_xref="PDB:2EEX" FT /db_xref="PDB:2EJ1" FT /db_xref="PDB:2EO7" FT /db_xref="PDB:2EQD" FT /db_xref="UniProtKB/TrEMBL:P71140" FT /protein_id="BAA12070.1" FT /translation="MAKRRLSLLLVLAIMFTMVVPQISASAETVAPEGYRKLLDVQIFK FT DSPVVGWSGSGMGELETIGDTLPVDTTVTYNGLPTLRLNVQTTVQSGWWISLLTLRGWN FT THDLSQYVENGYLEFDIKGKEGGEDFVIGFRDKVYERVYGLEIDVTTVISNYVTVTTDW FT QHVKIPLRDLMKINNGFDPSSVTCLVFSKRYADPFTVWFSDIKITSEDNEKSAPAIKVN FT QLGFIPEAEKYALVTGFAEELAVSEGDEFAVINAADNSVAYTGKLTLVTEYEPLDSGEK FT ILKADFSDLTVPGKYYISIEGLDNSPKFEIGEGIYGPLVVDAARYFYYQRQGIELEEPY FT AQGYPRKDVTPQDAYAVFASGKKDPIDITKGWYDAGDFGKYVNAGATGVSDLFWAYEMF FT PSQFVDGQFNIPESGNGVPDILDEARWELEWMLKMQDKESGGFYPRVQSDNDENIKSRI FT IRDQNGCTTDDTACAAGILAHAYLIYKDIDPDFAQECLDAAINAWKFLEKNPENIVSPP FT GPYNVYDDSGDRLWAAASLYRATGEEVYHTYFKQNYKSFAQKFESPTAYAHTWGDMWLT FT AFLSYLKAENKDQEVVDWIDTEFGIWLENILTRYENNPWKNAIVPGNYFWGINMQVMNV FT PMDAIIGSQLLGKYSDRIEKLGFGSLNWLLGTNPLRFSFVSGYGEDSVKGVFSNIYNTD FT GKQGIPKGYMPGGPNAYEGAGLSRFAAKCYTRSTGDWVANEHTVYWNSALVFMAAFANQ FT GSEVNPGPAPEPGVTPNPTEPAKVVDIRIDTSAERKPISPYIYGSNQELDATVTAKRFG FT GNRTTGYNWENNFSNAGSDWLHYSDTYLLEDGGVPKGEWSTPASVVTTFHDKALSKNVP FT YTLITLQAAGYVSADGNGPVSQEETAPSSRWKEVKFEKGAPFSLTPDTEDDYVYMDEFV FT NYLVNKYGNASTPTGIKGYSIDNEPALWSHTHPRIHPDNVTAKELIEKSVALSKAVKKV FT DPYAEIFGPALYGFAAYETLQSAPDWGTEGEGYRWFIDYYLDKMKKASDEEGKRLLDVL FT DVHWYPEARGGGERICFGADPRNIETNKARLQAPRTLWDPTYIEDSWIGQWKKDFLPIL FT PNLLDSIEKYYPGTKLAITEYDYGGGNHITGGIAQADVLGIFGKYGVYLATFWGDASNN FT YTEAGINLYTNYDGKGGKFGDTSVKCETSDIEVSSAYASIVGEDDSKLHIILLNKNYDQ FT PTTFNFSIDSSKNYTIGNVWAFDRGSSNITQRTPIVNIKDNTFTYTVPALTACHIVLEA FT AEPVVYGDLNNDSKVNAVDIMMLKRYILGIIDNINLTAADIYFDGVVNSSDYNIMKRYL FT LKAIEDIPYVPENQAPKAIFTFSPEDPVTDENVVFNASNSIDEDGTIAYYAWDFGDGYE FT GTSTTPTITYKYKNPGTYKVKLIVTDNQGASSSFTATIKVTSATGDNSKFNFEDGTLGG FT FTTSGTNATGVVVNTTEKAFKGERGLKWTVTSEGEGTAELKLDGGTIVVPGTTMTFRIW FT IPSGAPIAAIQPYIMPHTPDWSEVLWNSTWKGYTMVKTDDWNEITLTLPEDVDPTWPQQ FT MGIQVQTIDEGEFTIYVDAIDW" FT terminator 5453..5491 FT CDS 5963..>6465 FT /transl_table=11 FT /gene="celK" FT /product="endoglucanase K" FT /db_xref="GOA:P71141" FT /db_xref="HSSP:1G87" FT /db_xref="InterPro:IPR001701" FT /db_xref="InterPro:IPR008928" FT /db_xref="InterPro:IPR012341" FT /db_xref="UniProtKB/TrEMBL:P71141" FT /protein_id="BAA20861.1" FT /translation="MVKKTLCFVLTLAMLTAFILPQGIVSAAGSYNYAEALQKAIYFYE FT CQQAGPLPEWNRVEWRGDATMNDEVLGGWYDAGDHVKFNLPMAYSAAMLGWALYEYGDD FT IEASGQRLHLERNLAFALDYLVACDRGDSVVYQIGDGAADHKWWGSAEVIEKEMTRPYF FT VGKDP" XX SQ Sequence 6465 BP; 2044 A; 1182 C; 1455 G; 1784 T; 0 other; tttataaata tttataaaaa acatgtaaga ttgataaatg aaggcttttt atattatttt 60 ttgtaattat tgcattttgc ccccttaaaa aattttaaaa ttcgaaatta atttttgcgt 120 atggattgtg caataaaaat atgataatat ttaggtgaat ataaaaagat tgtattaatc 180 aattgcttac aggtataaaa attaagaaaa ttgcgcactg tgttggcgta accttaatca 240 acaattggaa atggggtttg ggaagtatac ttggaagcaa ctggttatta ttgaaattta 300 cagacgatgt gaaagactgt gaacaaacca atcaatgccc aaattatact ttgcagcaaa 360 atcatccttg catatccctt cagatttaaa agccgcgaca attagcgttt ccgatgaagc 420 tcatttatgt gaacctcgat tccttcctat aaaatttact ctattttcat ttcaattttt 480 ccagtttgca tgtggaatta tttcccgaag ctccgtattt ctgttgttga tggtttgtat 540 gtcaacatat gcgtgcattt taatataaaa tgaattgtta aaggagtgtg aaatatggca 600 aagagaagat tatcgctact tttggtactt gccataatgt ttacgatggt cgttccacag 660 atatctgcaa gtgccgaaac agttgctcct gaaggctaca ggaagctttt ggatgtacaa 720 attttcaagg attcgcctgt agtcggatgg tcaggaagcg gtatgggcga gcttgaaact 780 atcggcgata cccttccggt tgataccaca gttacatata acggtttgcc gactttaaga 840 ctgaatgtcc agacaaccgt tcagtcagga tggtggattt ctcttcttac attaagagga 900 tggaacaccc atgacctttc ccagtatgtc gaaaacggtt atcttgagtt tgacatcaag 960 ggtaaggaag gcggagaaga ctttgttatt ggtttcaggg acaaggttta tgaacgcgtt 1020 tacggacttg aaattgatgt taccacagta atatcaaatt atgtaacggt aactacggac 1080 tggcagcatg ttaagattcc tttgagagac ctgatgaaga ttaataacgg atttgatcct 1140 tcatcagtta catgcctggt gttctcaaaa agatatgcag atccgtttac agtatggttc 1200 agtgatataa agattacatc agaagacaat gaaaagtccg ctcctgcaat caaggtaaac 1260 cagcttggct ttattcctga agctgaaaaa tacgctttgg ttacaggttt tgcagaagag 1320 ctcgcagtat cggaaggtga cgaatttgcc gttataaatg ctgcggacaa ttctgttgct 1380 tataccggaa aattaactct tgtaacagaa tatgaacctc ttgattccgg agaaaaaata 1440 cttaaggcag atttcagcga cttgactgta cctggcaaat actacattag tattgaaggt 1500 cttgacaatt cacccaagtt tgaaatcggt gaaggtattt acggtccact ggttgttgac 1560 gctgcaagat atttctatta tcagcgtcag ggtatagaac ttgaagagcc ttatgcgcag 1620 ggatatcccc gcaaggacgt tactcctcag gacgcatatg ctgtatttgc atccggaaag 1680 aaggatccga ttgacataac aaagggttgg tatgacgcag gagacttcgg taagtatgta 1740 aatgccggag caaccggtgt ttccgatttg ttctgggcat atgaaatgtt cccttcccag 1800 tttgttgacg gtcagttcaa tattcctgaa agcggaaacg gtgtaccgga catccttgac 1860 gaagctcgct gggagcttga atggatgctg aaaatgcagg acaaagaaag cggaggattc 1920 tatcccagag ttcaatctga caatgacgaa aacataaaat caagaataat cagggatcag 1980 aacggctgta ccactgatga tactgcatgt gccgccggaa tacttgctca tgcatacttg 2040 atttacaagg atattgaccc tgattttgca caagagtgcc tggatgcggc aataaatgca 2100 tggaaattcc ttgaaaagaa tcctgaaaac attgtttcac ctccgggtcc atacaacgta 2160 tatgacgaca gcggagacag actctgggct gcagcttcgc tgtacagagc taccggtgaa 2220 gaggtttatc atacatactt taaacaaaac tacaaatctt ttgcacaaaa gttcgaaagc 2280 ccgactgcat atgctcatac atggggtgat atgtggctta cggcattcct ttcgtatttg 2340 aaagctgaaa acaaggatca ggaagttgta gactggattg atacagagtt tggaatctgg 2400 cttgaaaaca tactcacaag atatgagaac aatccatgga agaatgcaat tgttcccgga 2460 aactacttct ggggaatcaa catgcaggtt atgaatgttc cgatggatgc tatcataggt 2520 tcacagcttc ttggaaaata cagtgacaga atagaaaaat taggttttgg ttcacttaac 2580 tggctgcttg gtacaaatcc gcttcgcttc agctttgtat caggatatgg agaggattct 2640 gtaaaaggag tattcagcaa tatttacaat acggacggca agcagggaat tccgaaagga 2700 tacatgcctg gtggaccaaa tgcttatgaa ggtgcaggcc tgtcaaggtt tgcagcaaaa 2760 tgctacacca gaagtaccgg tgactgggta gccaacgaac atacagtata ttggaactca 2820 gctttggtat ttatggctgc ttttgcaaac cagggttcag aggttaatcc gggacctgcg 2880 ccggaaccgg gagtaactcc gaatcctaca gaacctgcaa aagtggttga catcaggata 2940 gatacttctg ctgaaagaaa gccaatcagc ccgtatatat acggaagcaa tcaggaactt 3000 gatgcaacag ttactgcaaa gaggttcggc ggaaacagaa ctacaggata caactgggaa 3060 aacaacttct caaatgcagg aagtgactgg ctgcattaca gtgatacata ccttttggag 3120 gacggcggag ttcctaaggg agagtggagt acacctgctt ctgtagttac cacgttccat 3180 gacaaggcac ttagcaaaaa tgttccttac acacttatca ctcttcaggc agcaggttat 3240 gtttccgcag acggaaacgg accggtttcc caggaagaaa ctgcaccgtc ttcaagatgg 3300 aaggaagtta agtttgaaaa gggagcacct ttctcactta caccggacac agaagatgat 3360 tatgtttaca tggatgagtt tgtaaactat cttgtaaaca aatacggaaa tgcatccaca 3420 cctacaggaa taaagggtta ttcaatagat aacgagccgg cattgtggag tcatactcat 3480 ccgagaattc atccggacaa tgtaactgcc aaagagctta ttgaaaaatc tgtagctctt 3540 tccaaggcgg ttaaaaaggt agatccatat gcagaaatat tcggacctgc tttgtacgga 3600 tttgccgcat atgagacact tcagtcagct cctgactggg gaactgaagg agaaggatac 3660 aggtggttta tagattatta cctcgataag atgaaaaagg cttctgatga agaaggaaag 3720 agacttttgg acgtacttga cgtacactgg tatccggaag ccaggggcgg cggtgaaaga 3780 atatgctttg gagccgatcc aagaaatatt gagacaaaca aagcaagatt gcaggcgccc 3840 agaacattgt gggatcctac atatattgaa gacagctgga taggacaatg gaagaaggat 3900 ttcctcccga tattacctaa tcttttggat tccattgaaa aatattatcc gggaacgaag 3960 cttgctataa ctgaatatga ctatggcgga ggaaatcata ttacaggcgg tattgctcaa 4020 gccgatgttc ttggtatatt cggtaaatac ggtgtttacc ttgcaacatt ctggggagat 4080 gcaagcaata actatactga ggccggtata aacctttata ccaactacga cggcaaaggc 4140 ggcaaatttg gagatacatc cgtaaaatgt gaaacgtccg acatagaagt aagctctgct 4200 tatgcatcca ttgtcggtga agatgacagc aaactccata tcattctttt gaacaagaac 4260 tatgaccagc cgacgacatt caatttctca attgacagca gcaagaacta cacaatagga 4320 aatgtatggg catttgacag aggaagctcc aatattactc aaagaactcc tatagtgaac 4380 ataaaggaca ataccttcac atatacagta ccggctttga cagcgtgcca tattgtgctt 4440 gaagctgcgg agcccgtagt gtacggagac ttgaacaatg actctaaagt aaacgcagta 4500 gacattatga tgctcaaacg atatattctc ggaataatag ataatataaa tctgacagca 4560 gctgacattt attttgacgg tgttgtaaat tcaagtgact ataatataat gaagagatat 4620 ttgttaaagg caatagaaga tattccttat gttccggaaa accaggcacc taaagcaata 4680 tttactttct cgcccgaaga cccggttact gacgagaatg tagtgttcaa tgcatcaaat 4740 tcaatagatg aagacggaac aattgcctat tatgcatggg atttcggtga cggatatgaa 4800 ggaacttcaa caacaccgac tattacctat aagtataaaa accccggaac atacaaagta 4860 aaactgattg ttacagacaa ccagggggct tcaagttcgt ttacagctac cataaaagta 4920 acctcagcta ccggggacaa ttccaaattc aactttgaag acggcacgct gggaggattt 4980 acaacatccg gaacaaatgc tacgggtgtt gttgtgaaca ctactgaaaa agcattcaaa 5040 ggcgaaagag gtcttaaatg gactgtaaca agcgaaggag aaggaactgc agaattgaaa 5100 cttgacggag gtactattgt agttcccggt accactatga cgtttagaat ctggatacct 5160 tccggtgcgc ctattgctgc catccagccg tatattatgc ctcatacacc tgattggtcg 5220 gaagtcctct ggaattcgac atggaaagga tacaccatgg tgaagaccga tgactggaat 5280 gaaattaccc tgacactgcc ggaagacgtg gatccgactt ggccgcagca gatgggtata 5340 caggtacaga ccatagatga aggtgaattc actatctatg tagatgctat tgactggtaa 5400 gaaatttaat tggtcaaaca aaaatcaaat cagttgcaag ttataaggcc gggcaaaaaa 5460 ggaggaacct ttgcggtttc tccttttttg cttttaaaat ttttttatcg agattagttc 5520 aaaaaacaaa aacgggaaac aggatttgac ccacttaaaa atgtgtatgt gcacggattc 5580 ctatttggag ggaagcagaa attcataaaa actttaaaaa tcttattaag tttggctgtg 5640 taaagagaga tggagttatg atataataat atttgaataa tattatttat atcattaaac 5700 agtttaaata aataagcact atgcaaaaca aaacgccaat cagtttccat ggtggtttcc 5760 gctttctata cagcaaatct atttacacat attctccatt ttgattgaag caagttttgt 5820 agttaaatgc tatgggaaag ttataatttt tatataactt tgttattgat tttttatatt 5880 tttcccttat ggaggtgatt ttgtaaaata caaacaataa atataatcaa atatattatt 5940 aaattatttt gaaggaggaa tcatggtgaa aaagacatta tgctttgtac tgactttggc 6000 tatgctgacg gcatttattc ttcctcaggg gattgtgtcc gcagcaggaa gctataacta 6060 tgcggaagca cttcagaaag ccatttactt ttatgagtgt cagcaggccg gccctctacc 6120 tgaatggaac cgcgttgagt ggcgtggcga cgcaacaatg aatgatgagg tacttggtgg 6180 atggtatgac gcaggtgacc atgtcaagtt taatctgcct atggcgtatt cggcggcaat 6240 gcttggctgg gctctttatg agtatggcga tgacattgag gcatcggggc agagacttca 6300 tcttgaaagg aaccttgcct ttgcccttga ctatcttgtt gcctgcgaca gaggtgacag 6360 tgtcgtttat cagataggtg acggtgccgc tgaccataaa tggtggggtt ctgcggaagt 6420 tattgaaaaa gaaatgacaa gaccttactt tgtaggaaag gatcc 6465 // |
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