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EBI DbfetchID D83245; SV 1; linear; genomic DNA; STD; PRO; 7218 BP. XX AC D83245; XX DT 26-MAR-1997 (Rel. 51, Created) DT 05-OCT-2006 (Rel. 89, Last updated, Version 7) XX DE Sulfolobus acidocaldarius DNA for maltooligosyltrehalose trehalohydrolase, DE maltooligosyltrehalose synthase, complete cds. XX KW glycogen debranching enzyme; maltooligosyltrehalose synthase; KW maltooligosyltrehalose trehalohydrolase; treX; treY; treZ. XX OS Sulfolobus acidocaldarius OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; OC Sulfolobus. XX RN [1] RP 1-7218 RA Maruta K.; RT ; RL Submitted (29-JAN-1996) to the EMBL/GenBank/DDBJ databases. RL Kazuhiko Maruta, Hayashibara Biochemical Laboratories, Inc., Amase RL Institute; Amase-minami-machi 7-7, Okayama, Okayama 700, Japan RL (E-mail:amaseh@po.harenet.or.jp, Tel:086-222-7776(ex.323), RL Fax:086-231-6738) XX RN [2] RP 1-7218 RA Maruta K., Mitsuzumi H., Nakada T., Kubota M., Chaen H., Fukuda S., RA Sugimoto T., Kurimoto M.; RT "Cloning and sequencing of a cluster of trehalose biosynthesis genes from RT thermophilic archaebacterium Sulfolobus acidocaldarius"; RL Unpublished. XX RN [3] RX DOI; 10.1016/S0304-4165(96)00082-7 RX PUBMED; 8980629. RA Maruta K., Mitsuzumi H., Nakada T., Kubota M., Chaen H., Fukuda S., RA Sugimoto T., Kurimoto M.; RT "Cloning and sequencing of a cluster of genes encoding novel enzymes of RT trehalose biosynthesis from thermophilic archaebacterium Sulfolobus RT acidocaldarius"; RL Biochim. Biophys. Acta 1291(3):177-181(1996). XX FH Key Location/Qualifiers FH FT source 1..7218 FT /organism="Sulfolobus acidocaldarius" FT /strain="ATCC33909" FT /mol_type="genomic DNA" FT /db_xref="taxon:2285" FT CDS 398..2068 FT /transl_table=11 FT /gene="treZ" FT /product="maltooligosyltrehalose trehalohydrolase" FT /db_xref="GOA:Q53641" FT /db_xref="HSSP:1EH9" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q53641" FT /protein_id="BAA11863.1" FT /translation="MFSFGGNIEKNKGIFKLWAPYVNSVKLKLSKKLIPMEKNDEGFFE FT VEIDDIEENLTYSYIIEDKREIPDPASRYQPLGVHDKSQLIRTDYQILDLGKVKIEDLI FT IYELHVGTFSQEGNFKGVIEKLDYLKDLGITGIELMPVAQFPGNRDWGYDGVFLYAVQN FT TYGGPWELAKLVNEAHKRGIAVILDVVYNHIGPEGNYLLGLGPYFSDRYKTPWGLTFNF FT DDRGCDQVRKFILENVEYWFKTFKIDGLRLDAVHAIFDNSPKHILQEIAEKAHQLGKFV FT IAESDLNDPKIVKDDCGYKIDAQWVDDFHHAVHAFITKEKDYYYQDFGRIEDIEKTFKD FT VFVYDGKYSRYRGRTHGAPVGDLPPRKFVVFIQNHDQVGNRGNGERLSILTDKTTYLMA FT ATLYILSPYIPLIFMGEEYYETNPFFFFSDFSDPVLIKGVREGRLKENNQMIDPQSEEA FT FLKSKLSWKIDEEVLDYYKQLINIRKRYNNCKRVKEVRREGNCITLIMEKIGIIASFDD FT IVINSKITGNLLIGIGFPKKLKKDELIKVNRGVGVYQLE" FT CDS 2065..4206 FT /transl_table=11 FT /gene="treX" FT /product="glycogen debranching enzyme" FT /db_xref="GOA:O05152" FT /db_xref="HSSP:1BF2" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:O05152" FT /protein_id="BAA11864.1" FT /translation="MKDRPLKPGEPYPLGATWIEEEDGVNFVLFSENATKVELLTYSQT FT RQDEPKEIIELRQRTGDLWHVFVPGLRPGQLYGYRVYGPYKPEEGLRFNPNKVLIDPYA FT KAINGLLLWDDSVFGYKIGDQNQDLSFDERKDDKFIPKGVIINPYFDWEDEHFFFRRKI FT PFKDSIIYETHIKGITKLRQDLPENVRGTFLGLASDTMIDYLKDLGITTVEIMPIQQFV FT DERFIVDKGLKNYWGYNPINYFSPECRYSSSGCLGNQVIEFKKLVNSLHNAGLEVIIDV FT VYNHTAEGNHLGPTLSFKGIDNSSYYMLDPKNKRYYIDFTGTGNTLNLSHPRVLQLVLD FT SLRYWVLEMHVDGFRFDLASALARQLYSVNMLSTFFVAIQQDPILSQVKLIAEPWDVGP FT GGYQVGNFPYLWAEWNGKYRDTIRRFWRGDPVPYEELANRLLGSPDLYAGSNKTPFASI FT NYITSHDGFTLQDLVSYNQKHNEANKLNNEDGMNENYSWNCGVEGETNDSNILYCREKQ FT RRNFVITLFVSQGIPMILGGDEIGRTQKGNNNAFCQDNETSWYDWNLDENRVRFHDFVR FT RLTNFYKAHPIFRRARYFQGKKLHGSPLKDVTWLKPDGNEVDDSVWKSPTNHIIYILEG FT SAIDEINYNGERIADDTFLIILNGASTNLKIKVPHGKWELVLHPYPHEPSNDKKIIENN FT KEVEIDGKTALIYRRIEFQ" FT CDS 4203..6365 FT /transl_table=11 FT /gene="treY" FT /product="maltooligosyltrehalose synthase" FT /db_xref="GOA:Q53688" FT /db_xref="InterPro:IPR013781" FT /db_xref="PDB:1IV8" FT /db_xref="UniProtKB/TrEMBL:Q53688" FT /protein_id="BAA11865.1" FT /translation="MISATYRLQLNKNFNFGDVIDNLWYFKDLGVSHLYLSPVLMASPG FT SNHGYDVIDHSRINDELGGEKEYRRLIETAHTIGLGIIQDIVPNHMAVNSLNWRLMDVL FT KMGKKSKYYTYFDFFPEDDKIRLPILGEDLDTVISKGLLKIVKDGDEYFLEYFKWKLPL FT TEVGNDIYDTLQKQNYTLMSWKNPPSYRRFFDVNTLIGVNVEKDHVFQESHSKILDLDV FT DGYRIDHIDGLYDPEKYINDLRSIIKNKIIIVEKILGFQEELKLNSDGTTGYDFLNYSN FT LLFNFNQEIMDSIYENFTAEKISISESIKKIKAQIIDELFSYEVKRLASQLGISYDILR FT DYLSCIDVYRTYANQIVKECDKTNEIEEATKRNPEAYTKLQQYMPAVYAKAYEDTFLFR FT YNRLISINEVGSDLRYYKISPDQFHVFNQKRRGKITLNATSTHDTKFSEDVRMKISVLS FT EFPEEWKNKVEEWHSIINPKVSRNDEYRYYQVLVGSFYEGFSNDFKERIKQHMIKSVRE FT AKINTSWRNQNKEYENRVMELVEETFTNKDFIKSFMKFESKIRRIGMIKSLSLVALKIM FT SAGIPDFYQGTEIWRYLLTDPDNRVPVDFKKLHEILEKSKKFEKNMLESMDDGRIKMYL FT TYKLLSLRKQLAEDFLKGEYKGLDLEEGLCGFIRFNKILVIIKTKGSVNYKLKLEEGAI FT YTDVLTGEEIKKEVQINELPRILVRM" XX SQ Sequence 7218 BP; 2680 A; 970 C; 1422 G; 2146 T; 0 other; ttggcccctg ggaccagggt tcgagtccct gcccggctac ctttgaaagg ttagggggat 60 acaccctaat accccacttc tatcttacaa tttcaggtaa gtctttacta ggtcaactaa 120 agcaccaacg taagtctcct tcgtcttacc accttgactc ttcttgataa agtaaacata 180 atatcatcca tagacttacc ttattcttat attaccatat gattttatta tttgggattt 240 ctattagata agtcccactc atagaacaaa tgatggtttt aacttatata ctaaatactc 300 taataactca acaataataa gaatttaatc agttctgata agtattttca ctcgaaaaca 360 tttaaatata ttaagacata atttctattt aaacagcatg ttttcgttcg gtggaaatat 420 tgaaaaaaat aaaggtatct ttaagttatg ggcaccttat gttaatagtg ttaagctgaa 480 gttaagcaaa aaacttattc caatggaaaa aaacgatgag ggatttttcg aagtagaaat 540 agacgatatc gaggaaaatt taacctattc ttatattata gaagataaga gagagatacc 600 tgatcccgca tcacgatatc aacctttagg agttcatgac aaatcacaac ttataagaac 660 agattatcag attcttgacc ttggaaaagt aaaaatagaa gatctaataa tatatgaact 720 ccacgttggt actttttccc aagaaggaaa tttcaaagga gtaatagaaa agttagatta 780 cctcaaggat ctaggaatca caggaattga actgatgcct gtggcacaat ttccagggaa 840 tagagattgg ggatacgatg gtgtttttct atacgcagtt caaaatactt atggcggacc 900 atgggaattg gctaagctag taaacgaggc acataaaagg ggaatagccg taattttgga 960 tgttgtatat aatcatatag gtcctgaggg aaattacctt ttaggattag gtccttattt 1020 ttcagacaga tataaaactc catggggatt aacatttaat tttgatgata ggggatgtga 1080 tcaagttaga aaattcattt tagaaaatgt cgagtattgg tttaagacct ttaaaatcga 1140 tggtctgaga ctggatgcag ttcatgcaat ttttgataat tcgcctaagc atatcctcca 1200 agagatagct gaaaaagccc atcaattagg aaaatttgtt attgctgaaa gtgatttaaa 1260 tgatccaaaa atagtaaaag atgattgtgg atataaaata gatgctcaat gggttgacga 1320 tttccaccac gcagttcatg cattcataac caaagaaaaa gattattatt accaggattt 1380 tggaaggata gaagatatag agaaaacttt taaagatgtt tttgtttatg atggaaagta 1440 ttctagatac agaggaagaa ctcatggtgc tcctgtaggt gatcttccac cacgtaaatt 1500 tgtagtcttc atacaaaatc acgatcaagt aggaaataga ggaaatgggg aaagactttc 1560 catattaacc gataaaacga cataccttat ggcagccaca ctatatatac tctcaccgta 1620 tataccgcta atatttatgg gcgaggaata ttatgagacg aatccttttt tcttcttctc 1680 tgatttctca gatcccgtat taattaaggg tgttagagaa ggtagactaa aggaaaataa 1740 tcaaatgata gatccacaat ctgaggaagc gttcttaaag agtaaacttt catggaaaat 1800 tgatgaggaa gttttagatt attataaaca actgataaat atcagaaaga gatataataa 1860 ttgtaaaagg gtaaaggaag ttaggagaga agggaactgt attactttga tcatggaaaa 1920 aataggaata attgcatcgt ttgatgatat tgtaattaat tctaaaatta caggtaattt 1980 acttataggc ataggatttc cgaaaaaatt gaaaaaagat gaattaatta aggttaacag 2040 aggtgttggg gtatatcaat tagaatgaaa gatcgaccat taaagcctgg tgaaccttat 2100 cctttagggg caacttggat agaggaagaa gatggagtta attttgtact attctctgag 2160 aacgccacaa aagtagaact gttaacgtac tctcagacta gacaagatga gccaaaggaa 2220 ataatagaac ttagacagag aaccggagat ctctggcatg tttttgtacc tggtttaaga 2280 ccaggtcagt tgtatgggta cagggtgtat ggtccatata aaccagagga agggttaagg 2340 tttaatccta ataaagtact gatagatcct tatgcaaaag ctataaacgg attattacta 2400 tgggatgatt cggtctttgg atataaaatt ggagatcaga accaggatct cagtttcgat 2460 gagagaaaag acgataaatt tatacctaaa ggggtcataa taaatcctta ttttgattgg 2520 gaggacgagc atttcttctt tagaagaaag atacctttta aggatagtat aatttatgag 2580 acacatataa aaggaataac taaattaagg caagatttac cggagaacgt tagaggcact 2640 tttttgggtt tagcatcaga tactatgatt gattacctaa aagatttagg aattacaacc 2700 gttgagataa tgcctattca gcaatttgta gatgagagat tcattgtcga taaagggtta 2760 aagaactact ggggttacaa tccgataaat tatttctctc ctgaatgtag atactcaagc 2820 tcaggctgtt taggaaatca ggtcattgaa tttaagaagt tggtaaattc tttgcataac 2880 gctgggttag aggtaataat agacgttgta tataaccata cagcagaagg aaaccatctt 2940 ggacctactt tgagtttcaa aggaatagat aatagttcgt attacatgct ggaccctaaa 3000 aataaacgtt attatattga cttcacagga acagggaata cgcttaattt gtctcatcca 3060 agagttttgc aattggtttt ggatagctta cgctactggg ttctcgagat gcatgtagat 3120 ggtttcagat ttgatctcgc atctgcccta gcaagacaac tatatagcgt gaatatgtta 3180 tcgactttct ttgttgctat acaacaggat cctatcttat cacaagttaa gctgatagca 3240 gagccgtggg atgtaggacc tggcggttat caagtaggta attttccata tctatgggct 3300 gaatggaatg gtaaatatag ggatacaata agaagattct ggagagggga tccagtacct 3360 tatgaggagt tagcaaacag gttattaggg tctcctgacc tttatgcagg tagtaataaa 3420 actccttttg ccagtataaa ttacattact tctcacgacg gctttacatt acaagattta 3480 gtaagttata atcagaagca taatgaggct aataaactga acaatgagga cggaatgaat 3540 gaaaattata gctggaattg tggagtagaa ggagaaacta acgattctaa tattctttat 3600 tgtagagaaa aacaaagaag aaattttgta ataacattat ttgttagcca aggtatacca 3660 atgatcttag ggggagacga aataggaaga acacaaaaag gcaacaataa tgctttttgt 3720 caggataatg agacaagttg gtatgattgg aaccttgatg aaaatcgtgt aaggtttcat 3780 gattttgtga ggagacttac caatttttat aaagctcatc cgatatttag gagggctaga 3840 tattttcagg gtaagaagtt acacggttcc ccattaaagg atgtgacgtg gctaaaacct 3900 gacggcaatg aagttgatga ttcagtgtgg aaatctccaa caaatcatat tatttatata 3960 ttagagggaa gtgctatcga tgaaataaat tataatggag aaaggatagc tgacgacact 4020 tttctaatta ttttgaatgg agcaagtact aatcttaaga taaaagtacc tcatggaaaa 4080 tgggagttag tgttacatcc ttatccacat gagccatcta acgataaaaa gataatagaa 4140 aacaacaaag aagtagaaat agatggaaag actgcactaa tttacaggag gatagagttc 4200 cagtgatatc agcaacctac agattacagt taaataagaa ttttaatttt ggtgacgtaa 4260 tcgataacct atggtatttt aaggatttag gagtttccca tctctacctc tctcctgtct 4320 taatggcttc gccaggaagt aaccatgggt acgatgtaat agatcattca aggataaacg 4380 atgaacttgg aggagagaaa gaatacagga gattaataga gacagctcat actattggat 4440 taggtattat acaggacata gtaccaaatc acatggctgt aaattctcta aattggcgac 4500 taatggatgt attaaaaatg ggtaaaaaga gtaaatatta tacgtacttt gactttttcc 4560 cagaagatga taagatacga ttacccatat taggagaaga tttagataca gtgataagta 4620 aaggtttatt aaagatagta aaagatggag atgaatattt cctagaatat ttcaaatgga 4680 aacttcctct aacagaggtt ggaaatgata tatacgacac tttacaaaaa cagaattata 4740 ccctaatgtc ttggaaaaat cctcctagct atagacgatt cttcgatgtt aatactttaa 4800 taggagtaaa tgtcgaaaaa gatcacgtat ttcaagagtc ccattcaaag atcttagatt 4860 tagatgttga tggctataga attgatcata ttgatggatt atatgatcct gagaaatata 4920 ttaatgacct gaggtcaata attaaaaata aaataattat tgtagaaaaa attctgggat 4980 ttcaggagga attaaaatta aattcagatg gaactacagg atatgacttc ttaaattact 5040 ccaacttact gtttaatttt aatcaagaga taatggacag tatatatgag aatttcacag 5100 cggagaaaat atctataagt gaaagtataa agaaaataaa agcgcaaata attgatgagc 5160 tatttagtta tgaagttaaa agattagcat cacaactagg aattagctac gatatattga 5220 gagattacct ttcttgtata gatgtgtaca gaacttatgc taatcagatt gtaaaagagt 5280 gtgataagac caatgagata gaggaagcaa ccaaaagaaa tccagaggct tatactaaat 5340 tacaacaata tatgccagca gtatacgcta aagcttatga agatactttc ctctttagat 5400 acaatagatt aatatccata aatgaggttg gaagcgattt acgatattat aagatatcgc 5460 ctgatcagtt tcatgtattt aatcaaaaac gaagaggaaa aatcacacta aatgccacta 5520 gcacacatga tactaagttt agtgaagatg taaggatgaa aataagtgta ttaagtgaat 5580 ttcctgaaga atggaaaaat aaggtcgagg aatggcatag tatcataaat ccaaaggtat 5640 caagaaatga tgaatataga tattatcagg ttttagtggg aagtttttat gagggattct 5700 ctaatgattt taaggagaga ataaagcaac atatgataaa aagtgtcaga gaagctaaga 5760 taaatacctc atggagaaat caaaataaag aatatgaaaa tagagtaatg gaattagtgg 5820 aagaaacttt taccaataag gatttcatta aaagtttcat gaaatttgaa agtaagataa 5880 gaaggatagg gatgattaag agcttatcct tggtcgcatt aaaaattatg tcagccggta 5940 tacctgattt ttatcaggga acagaaatat ggcgatattt acttacagat ccagataaca 6000 gagtcccagt ggattttaag aaattacacg aaatattaga aaaatccaaa aaatttgaaa 6060 aaaatatgtt agagtctatg gacgatggaa gaattaagat gtatttaaca tataagcttt 6120 tatccctaag aaaacagttg gctgaggatt ttttaaaggg cgagtataag ggattagatc 6180 tagaagaagg actatgtggg tttattaggt ttaacaaaat tttggtaata ataaaaacca 6240 agggaagtgt taattacaaa ctgaaacttg aagagggagc aatttacaca gatgtattga 6300 caggagaaga aattaaaaaa gaggtacaga ttaatgagct acctaggata ctagttagaa 6360 tgtaagttat aataatccga tttttatgtg acaagattta cgcttacgaa aaggactgtt 6420 aaatcaactt ttatgtgaat tatgaaacgt aaattataag tttcctgagg ataaacatat 6480 atatctctat ctctcattga tatcacatga gtattagatt aaggggaagt aattcttacg 6540 gacattcagg ctggtttaca gtatactgta gaatatgtaa taggaaaata agaataggaa 6600 cggacttagt ctacaaatgc cctaaatgtg aaaagaagta taacgcattc ttctgtgaag 6660 cagatgctag gggattaaag aaaaagtgcc catactgtgg tactgaactt gtcagtgcaa 6720 tttaagactc aaatagaagg taaaaatatt tttatactga ataatgagtt gttttacgct 6780 gatacggata tagttattcg aaatcaagat tttattaaga aactcacctt tacacaatat 6840 aataagattg cctatattga catggacata gaaacgacag aatttaagat attaagatta 6900 gtagtgtgta aaactagaat aaatatttat gtttgcaacg taattggtaa attgaaagaa 6960 actaattttg aaaatctaga ggagatttat ggtaaaatat tagaggagat tgataaagtt 7020 ataagataac ctttactttt cagcttagat gatacctcat ttattaactc cgcaagtttt 7080 ttttgcatta gtagaggaaa atctgacatg tatttccagt ttcctcaact gattaaaaat 7140 tgtaaaaacg actatcaaag aatcatcaga aggagaacat actaaagaac cacctgttct 7200 caccacacta caaagatc 7218 // |
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