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EBI DbfetchID D78258; SV 1; linear; genomic DNA; STD; PRO; 6142 BP. XX AC D78258; XX DT 09-NOV-1996 (Rel. 49, Created) DT 04-JUN-1999 (Rel. 59, Last updated, Version 3) XX DE Bacillus sp. DNA for alkaline amylopullulanase, complete cds. XX KW alkaline amylopullulanase. XX OS Bacillus sp. OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-6142 RA Hatada Y.; RT ; RL Submitted (07-NOV-1995) to the EMBL/GenBank/DDBJ databases. RL Yuji Hatada, Kao Corporation, Tochigi Research Laboratories; 2606 Akabane, RL Ichikai, Haga-gun, Tochigi 321-3497, Japan (Tel:0285-68-7400, RL Fax:0285-68-7403) XX RN [2] RP 1-6142 RA Hatada Y.; RT ; RL Unpublished. XX RN [3] RX DOI; 10.1074/jbc.271.39.24075 RX PUBMED; 8798645. RA Hatada Y., Igarashi K., Ozaki K., Ara K., Hitomi J., Kobayashi T., RA Kawai S., Watabe T., Ito S.; RT "Amino acid sequence and molecular structure of an alkaline RT amylopullulanase from Bacillus that hydrolyzes alpha-1,4 and alpha-1,6 RT linkages in polysaccharides at different active sites"; RL J. Biol. Chem. 271(39):24075-24083(1996). XX FH Key Location/Qualifiers FH FT source 1..6142 FT /organism="Bacillus sp." FT /strain="KSM-1378" FT /mol_type="genomic DNA" FT /db_xref="taxon:1409" FT CDS 145..5961 FT /codon_start=1 FT /transl_table=11 FT /product="alkaline amylopullulanase" FT /db_xref="GOA:P70983" FT /db_xref="InterPro:IPR019948" FT /db_xref="UniProtKB/TrEMBL:P70983" FT /protein_id="BAA11332.1" FT /translation="MKKRFQRGMAGLLSILLIVSMFAGYLPARAAAETGDKRIEFSYER FT PDGNYEGWNLWVWGTGVKDDQIDFTEFKEGKAYADIAVSDNADKVGFIIRKGDWEEKDF FT DGDRSITINKIDNITKVHVTSQQEKFGQIPDGSPPVVADGNADFFFRDKELYAAGEMDK FT VEKVELSILGEKYEMNGEPEKERFTYTLSDLPTGEHEYTYLVTVDGQTEEVTDPYNTVD FT GRSVVEYVTSDVQVSASFIPAKVDYNQNAVVKVDIESETETKIREMSINLSEIGGKEKA FT TIDPALNELTVAVKQGVTAGVKNLPITAIDEFGNRHEGSATLEVQARTITGEKADFDWD FT QSVVYFMLTDRFFDGDSSNNDPHGIGYDTSKSGTYQGGDFKGITQRLDYLDELGINTIW FT ISPVVDNIKFDVRHSEGPDTPYYAYHGYWADNFGELNPHFGSMADFHEMIDAAHERGIK FT IMVDVVLNHTGYGLKPGDSSSVANFPTDEDRARFDGMLRDGGSGEVRGELAGLPDFLTE FT NPDVREQVVQWQTDWIEKSRTAKGNTIDYFRVDTVKHVEDTTWMAFKNALTKAMPEHKL FT IGEAWGANVNDDLGYLNSGMMDSLLDFDFKNYARDFANGQLDAVQQKLEARNSKLNNTA FT TLGQFLGSHDEDRFYEVVEGDLGKYQVAASLQLTAKGQPVIYYGEELGLPGKNDYPYYT FT NRQNMPWDDVDGNEILEHYQKLLAFRNDNPNTFAKGDRKKVAGSDSEGYLLFSRTYGEN FT SVYVGLNTEAAAKDVTLNFGSSEAVVTDRYSGQEYQANEEGQVTFSIPAMEDGGTVLLE FT VENGAVPPVEEEPTEPGEIEENTLRIHYQRTDNSYENLGLWLWGDVAAPSENWPSGGTP FT FQAGNVTDYGAYVDVELAEDAQNIGFLVLNTTNGDKDGGDKAVELFSPDLNEIWIKQGS FT DEVFLYEPVDLPANTVRIHYERTNADYEGWGLWNWEDVESPSDGWPNGAADAAGIGKYG FT AYYDIKLKEDANKIGFLFVNKQSGGQTGDMTFDMLKQYNQLFVKEGEDKVYTNPYGTVP FT LALVSGEVLSDKLISLTFTRTEGLDLEELKEQLEIKDVDGNDVSFTDVTIEGEKTVHVH FT GEFDLEKIPFSVTYLDRTISVKSGWKLIDEMYAYDGKLGAELHEDGTATLKVWSPKADN FT VSVVLYDKVDQNEVVDTIEMVKGDRGVWSVKLTKDNTGLDSLKGYYYHYEITHGDVTNL FT ALDPYAKSMAAWNNEAGDKVGKAAIVDIGSIGPELDYADIPGFEKREDTIIYEVHVRDF FT TSDPNIGEDLKAQFGTFASFVEKLDYIQELGVTHIQLLPVMSYYFSNEFESGERMLEYA FT STGTNYNWGYDPHNYFSLSGMYSENPEDPELRIKEFKNLINEIHKRDMGVVLDVVFNHT FT AQVHIFEDLVPNYYHFMDADGTPRTSFGGGRLGTTHEMSRRVLVDSIKHWVDEYKVDGF FT RFDMMGDHDAESIQIAFDEAKKLNPNIVMIGEGWVTFAGDEGEPVQAADQQWMQYTEAV FT GSFSDEFRNELKSGFGSEGQPRFITGGAVNVQQIFDNIKAQPHNFMADQPGDVVQYIEA FT HDNLTLYDVIAQSIKKDPEIAENDLEIHKRIRVGNAMVLTSQGTAFLHAGQEFGRTKQW FT RAPATEAPYKSTYMTDADGNPFVYPYFIHDSYDSSDIINRFDWEKATDAEKYPVNNVTR FT DYTAGLIELRRSSDAFRLGSRELVDSNVTMVDAPEIKEQDLVVAYRSVSTAGVEYYTFV FT NADTSSRTLTLGQDLTEGVVVVDAEEANVAGVAEPAGFELTAEGITLEPLTTVVVRVGE FT QEGTDPGDGDGDGNTPPPGDGDGDGNTPPPGDGDGDGNTPPPGNGNGNNPGTPPGKGGE FT NPGKGKNDKTPPGKGGDNPGKGNKLPLTATGTLNYILFGAIMLVLGTLLYLGVRRKAGL FT KEKTL" XX SQ Sequence 6142 BP; 1781 A; 1224 C; 1646 G; 1491 T; 0 other; tctagatgtg caattttgcg caaacgattt cacatttaca taaacaatct tggcatcaat 60 taaattattt attgtgcaac tttgtgcaaa cgcttccaca ttttagcaag aaatgcaaat 120 cattgtatgg aaaggggcag ggatatgaag aaaaggtttc aaaggggtat ggctggttta 180 ctttctattt tacttattgt ttccatgttt gcaggctatc taccggcaag agcagcggcc 240 gaaacgggag acaagcggat agaattcagt tatgaacggc cagatggaaa ttatgaaggc 300 tggaatttat gggtctgggg aactggtgtg aaggatgacc agatagactt tacagaattc 360 aaggaaggca aggcatatgc cgacatagca gtaagcgata atgcggataa agtcggtttc 420 attatccgta aaggggattg ggaagaaaag gactttgatg gggacaggtc gattacgatc 480 aataagatcg ataacatcac caaagtgcat gtaacaagcc agcaggaaaa attcgggcaa 540 attcctgacg gcagcccacc tgttgttgcg gacgggaatg ctgacttctt tttccgtgat 600 aaagaactgt acgcagcagg agaaatggat aaggttgaga aagtcgaact gtccatttta 660 ggcgaaaaat acgagatgaa tggtgagccg gaaaaggagc gttttacata tacattaagc 720 gatcttccta caggcgagca tgaatatact tatttggtga cagtggatgg acagacagag 780 gaagttaccg atccatataa cacggtggat ggaaggtctg ttgtggagta tgtgacatcc 840 gatgtgcaag tatcggcttc atttataccc gcaaaggttg attataacca gaacgctgtg 900 gtgaaggtag acatcgaatc agaaacggag acaaaaatcc gtgagatgtc tatcaatctt 960 tcagaaatcg gcggcaaaga gaaagcaacc attgatcctg cgctgaatga attgacagtt 1020 gcggtcaagc aaggtgtgac ggcaggtgtg aaaaacttgc ctatcactgc gattgatgaa 1080 ttcggaaatc gccatgaggg atctgctacc ttagaagttc aggcgcgtac tattacaggt 1140 gaaaaagcag atttcgactg ggatcagtct gtggtttatt ttatgctgac agatcgattc 1200 tttgatgggg attcatcgaa caatgaccct catggtattg gctatgacac aagcaagtct 1260 ggtacatacc aaggcggaga ttttaagggg atcacgcaaa ggcttgatta cttggacgag 1320 cttggaatca atacgatctg gatcagtccg gttgtcgata atatcaaatt tgatgttcga 1380 cacagtgaag gacctgatac accatattat gcttaccacg gctattgggc ggataatttc 1440 ggggaattga acccgcattt cggttccatg gcggatttcc atgaaatgat tgatgcggca 1500 catgaacgcg gcattaaaat catggttgat gtggtgttga atcacactgg ttatggattg 1560 aaaccaggtg acagcagcag tgtggcgaac ttcccgacag atgaggaccg agctcgcttt 1620 gacggaatgc ttcgtgatgg cggatctggt gaagttcgag gcgagcttgc tggccttcca 1680 gattttctga cggaaaaccc ggatgtccgt gaacaggtgg tgcaatggca gacggactgg 1740 atcgaaaagt ccaggacggc aaagggcaac accatcgatt atttccgtgt cgacaccgtc 1800 aagcatgtgg aagacaccac ttggatggcg tttaaaaatg ctttgacaaa agcgatgccg 1860 gaacacaagc tgattgggga agcatgggga gcaaatgtca atgacgacct aggttatctg 1920 aacagcggaa tgatggattc tttactggat tttgatttca aaaattatgc ccgtgacttt 1980 gcaaacggac agctggatgc ggttcagcaa aaacttgagg cgcgtaacag caagttgaac 2040 aatactgcaa cacttggtca atttttagga agccatgacg aagaccgctt ctatgaggtg 2100 gtggaaggag accttggcaa gtatcaagtt gctgcatccc ttcaactgac ggcaaagggt 2160 cagcctgtta tctattacgg agaagagctg ggcttgcctg gtaagaacga ttatccgtat 2220 tatacgaacc gccagaacat gccttgggat gatgtggatg gtaatgaaat tctagagcat 2280 tatcaaaaat tactggcatt ccgtaatgat aatccgaaca catttgctaa aggagaccgc 2340 aaaaaggtag cgggatctga cagtgaagga tatcttttat tttcacggac gtacggggaa 2400 aattccgttt atgtaggttt gaatacggaa gctgctgcga aagacgtaac cttgaacttc 2460 ggttcttcag aagcagtggt gacggaccgc tattccggtc aggagtacca agcaaatgaa 2520 gaaggccaag tgacgttctc tattccggcg atggaagacg ggggaacggt cctgcttgaa 2580 gtggaaaatg gagcagtgcc acctgtggag gaagaaccaa ctgagccagg tgaaatcgaa 2640 gaaaacacgc ttcggattca ctaccagcgc acagacaaca gctacgaaaa ccttggtcta 2700 tggttatggg gagacgtcgc ggcaccatct gaaaactggc catcaggcgg cacaccgttc 2760 caagcaggta atgtaacaga ctatggtgca tatgtcgatg tggaattggc agaagatgct 2820 caaaatattg gattccttgt tttgaacacc acaaacggtg acaaggacgg cggcgacaaa 2880 gcagtagaat tgttcagtcc ggatttaaat gagatttgga tcaaacaagg ctctgatgaa 2940 gtatttttat atgaaccggt ggaccttccg gcaaatacgg tccgcattca ttatgaaaga 3000 accaatgccg actatgaagg ctgggggtta tggaactggg aggatgtcga gtccccatct 3060 gacgggtggc cgaacggtgc cgcagatgct gcaggtatcg gtaaatacgg tgcttactac 3120 gacatcaagc tgaaagaaga tgctaataaa attggtttcc tttttgtgaa caaacaatct 3180 ggtggccaaa cgggagatat gacgtttgat atgctgaaac aatacaacca actttttgta 3240 aaagagggcg aggacaaggt ctacaccaat ccttacggga ccgtgccatt ggcgcttgtg 3300 tctggagagg tattgtcaga caagttgatc agtcttactt ttaccaggac agaaggattg 3360 gatttggagg aattgaaaga acagctagaa atcaaggatg tggacgggaa cgatgtttcg 3420 tttacagatg tgacaattga aggcgagaaa acggtccatg tccacggcga gtttgacttg 3480 gagaaaatcc cgttctctgt gacctatctg gaccgcacca tttctgtaaa atcaggctgg 3540 aaactgatcg acgaaatgta tgcctatgat ggaaagcttg gggcagaatt gcatgaagac 3600 gggacggcta ctttgaaagt atggtcgcca aaagcggaca atgtgtctgt tgtactttat 3660 gacaaagttg accagaacga ggttgtagac accattgaaa tggtaaaagg ggaccgcggt 3720 gtctggtctg taaagctaac taaggataat acaggccttg atagtttgaa aggttactat 3780 taccactatg aaatcacgca tggtgacgta acgaatcttg ctctagatcc gtatgccaaa 3840 tcaatggcgg cgtggaataa cgaagcgggg gacaaggtag gaaaagcggc gatcgtggac 3900 atcggctcca ttgggcctga gcttgattat gccgacatcc ctggctttga aaagcgcgaa 3960 gacaccatca tctacgaggt gcatgtacgt gacttcactt ccgacccgaa tatcggtgag 4020 gacctgaagg cacagttcgg tacatttgct tctttcgtgg aaaagctgga ttacattcaa 4080 gagttaggtg tcactcacat tcaattgttg cctgtgatga gctattattt cagcaatgaa 4140 tttgagtctg gggagcgcat gctggagtat gcttcaacgg ggacgaatta caattggggc 4200 tatgacccgc acaattactt ctccttatcc ggcatgtact ccgaaaaccc tgaggacccg 4260 gaactgagaa tcaaagaatt caagaatctg atcaacgaga ttcataagcg cgacatgggt 4320 gtggtacttg atgtggtgtt taaccacacc gcacaggttc acattttcga ggaccttgta 4380 ccaaactact atcacttcat ggatgcggac ggaaccccaa gaactagctt tggcggtgga 4440 cgtcttggaa cgacacatga aatgtcccgc cgtgtgctcg tagattccat caagcattgg 4500 gtggatgaat ataaggtgga cggattccgt tttgacatga tgggtgacca tgatgcagag 4560 agtattcaga ttgcttttga cgaagccaaa aaattgaacc cgaatatcgt catgatcggg 4620 gaaggctggg taacatttgc tggtgacgag ggcgagccgg tccaggcggc cgatcaacaa 4680 tggatgcaat ataccgaagc agtgggtagc ttctcggatg aattccgcaa cgagctgaaa 4740 tccggtttcg gaagcgaagg acagccacgt ttcatcacag gtggcgcggt caatgtgcaa 4800 caaattttcg ataacatcaa agcacagcct cataacttta tggccgatca accaggcgat 4860 gtggtccaat acatcgaggc ccatgacaac ctgacgttat acgatgtcat cgcacaatct 4920 atcaaaaaag atccggaaat cgcggaaaac gatttagaga ttcataagcg tattcgcgtg 4980 ggtaatgcca tggtcttgac gtctcaaggt acggcattct tacacgcagg acaggaattt 5040 ggtcgtacaa agcaatggag agcacctgca acggaggcac cgtacaagtc tacgtatatg 5100 acagatgctg atggcaatcc gttcgtgtat ccatatttca tccacgattc ctatgattcc 5160 tcggatatca tcaatcgttt tgattgggaa aaagcgacag atgccgagaa ataccctgtc 5220 aacaatgtga cacgtgacta cacggcaggc ttgatcgagc tgcgtcgttc atctgatgct 5280 ttccgtttag gttctcgtga attggtcgat tccaatgtga caatggttga tgccccggaa 5340 atcaaggagc aggatctcgt tgttgcctac cgcagtgttt cgactgccgg tgtggagtat 5400 tacacattcg tgaatgcgga cacttccagt agaacattga ccttagggca ggatttgaca 5460 gagggcgtag tggtggtcga tgcagaagag gctaatgtag ccggtgtagc tgagcctgct 5520 ggtttcgaat tgacggcaga aggcatcaca cttgagccat tgactacggt tgtcgtccgt 5580 gtaggcgagc aggaagggac agacccgggt gatggggacg gcgatggcaa tacgccgcca 5640 ccaggcgacg gcgatggcga tggaaacacg ccaccaccag gggatgggga tggcgatgga 5700 aacacgcctc ctccaggcaa cggtaatggc aataatccag gaacaccacc aggaaagggt 5760 ggagaaaacc ctggtaaagg caaaaacgac aaaacaccgc ctggcaaagg tggggacaat 5820 ccaggtaagg ggaacaagct accacttacc gcaaccggaa cacttaatta catcctgttt 5880 ggtgcaataa tgttggttct tgggacgctg ctgtatctag gggtcagaag aaaagcagga 5940 ttgaaagaaa aaaccttata aaaacaacgg aaaagtgtgg caggggaata tcccgccaca 6000 ctttttcgtt attataaggc attatttgct tgtagattaa ggattcgcta taggttattt 6060 tgtgtaacgt acattacttt tccgttgggc catatttatt ttccataccg ctcatttttc 6120 ttttccattg ggaccacatt ta 6142 // |
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