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EBI DbfetchID D49537; SV 1; linear; genomic DNA; STD; PRO; 5531 BP. XX AC D49537; XX DT 15-MAR-1995 (Rel. 43, Created) DT 19-JUL-2007 (Rel. 92, Last updated, Version 10) XX DE Clostridium perfringens pbg gene for beta-galactosidase, complete cds. XX KW . XX OS Clostridium perfringens OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-5531 RA Shimizu T.; RT ; RL Submitted (09-MAR-1995) to the EMBL/GenBank/DDBJ databases. RL Tohru Shimizu, University of Tsukuba, Institute of Basic Medical Sciences; RL 1-1-1, Tennohdai, Tsukuba, Ibaraki 305, Japan RL (E-mail:tshimizu@md.tsukuba.ac.jp, Tel:81-298-53-3354, Fax:81-298-53-3039) XX RN [2] RP 1-5363 RX PUBMED; 8577281. RA Shimizu T., Kobayashi T., Ba-Thein W., Ohtani K., Hayashi H.; RT "Sequence analysis of flanking regions of the pfoA gene of Clostridium RT perfringens: beta-galactosidase gene (pbg) is located in the 3'-flanking RT region"; RL Microbiol. Immunol. 39(9):677-686(1995). XX DR StrainInfo; 7953; NCTC 8237. XX FH Key Location/Qualifiers FH FT source 1..5531 FT /organism="Clostridium perfringens" FT /strain="NCTC8237" FT /mol_type="genomic DNA" FT /clone="pTSD101, pTSD201, pTSD301, pTSD401" FT /db_xref="taxon:1502" FT CDS <1..32 FT /codon_start=3 FT /transl_table=11 FT /gene="pfoA" FT /product="perfringolysin O" FT /db_xref="GOA:Q0TUS0" FT /db_xref="InterPro:IPR001869" FT /db_xref="UniProtKB/Swiss-Prot:Q0TUS0" FT /protein_id="BAA08481.1" FT /translation="YPGSSITYN" FT RBS 213..219 FT CDS 225..683 FT /codon_start=1 FT /transl_table=11 FT /gene="ORF18" FT /product="hypothetical protein" FT /db_xref="UniProtKB/TrEMBL:Q46250" FT /protein_id="BAA08482.1" FT /translation="MKLAEALILRADLQKELMSLKNRINQNLLVQEGEKPSENPYTLYD FT EILLKVSELEGLILKINKTNSNYITSNNESISDLIVKRDMLGKKISILKEIAEEATVKV FT NRYSQTEIKIFSAMKVDQLQREINKYSKEYRELDTKLQGLNWTIDLME" FT RBS 745..749 FT CDS 755..973 FT /codon_start=1 FT /transl_table=11 FT /gene="ORF9" FT /product="hypothetical protein" FT /db_xref="UniProtKB/TrEMBL:Q46251" FT /protein_id="BAA08483.1" FT /translation="MLVAFIKEVINPNLGKKVIIHVSRSRYLFYNLCSNKLHGYYLRIT FT IYNHMTINNKRGWGKGIQFKEDRVMIH" FT repeat_region 1585..1630 FT /rpt_type=INVERTED FT /rpt_unit_range=1620..1630 FT /rpt_unit_range=1585..1597 FT repeat_region 1600..1630 FT /rpt_type=INVERTED FT /rpt_unit_range=1600..1610 FT /rpt_unit_range=1620..1630 FT repeat_region 1688..1702 FT /rpt_type=INVERTED FT /rpt_unit_range=1688..1693 FT /rpt_unit_range=1697..1702 FT RBS 1726..1733 FT CDS 1742..3229 FT /codon_start=1 FT /transl_table=11 FT /gene="ORF54" FT /product="membrane-spanning transporter protein" FT /db_xref="GOA:Q46252" FT /db_xref="InterPro:IPR002293" FT /db_xref="InterPro:IPR004841" FT /db_xref="UniProtKB/TrEMBL:Q46252" FT /protein_id="BAA08484.1" FT /translation="MSERKRSLSSGALMLMTFTAVFSFGNIIDSSVNIGLATIPSYIFG FT TVFYFLPFALMIGEFASASSDSESGINSWIKKSLGARWAFLGSWSYFFVNLFFFTSLLP FT KILIYASYTFVGRNVFDGKTVLISVISIVLFWAVTIISTKGVSWISKITSISGVARIIL FT GLGFIVLSFGVILFLGKAPAQEFTAETIMPKFNWSYFMVLAWILQAVGGAESIGVYIKD FT VKGGNKTFIRTMVISTAIVGGLYALGAVSVGLVVPSEVLQGNFSNGLFDAFAILGANYG FT VGNIITNIVGFIMMLASLGSLVLWTAAPVKVLFSEIPEGIFGKWIAKTDKKGTPVNALY FT VQAVIVTVLLLVPALGIGSVDSLLEMLINMTASTSLIPVLFFLVGYIVLRAKKDHMERS FT FKVGSKNFGIAIGVLLLALFVFVFVISSIPAPQDFAAYFNGTLAEGATNPVFILLYNVL FT GLVFFLGFAEICWRKYEKKVGKAVANEWDQEEVSEIA" FT repeat_region 3266..3297 FT /rpt_type=INVERTED FT /rpt_unit_range=3266..3279 FT /rpt_unit_range=3284..3297 FT RBS 3415..3418 FT CDS 3427..5457 FT /codon_start=1 FT /transl_table=11 FT /gene="pbg" FT /product="beta-galactosidase" FT /EC_number="3.2.1.23" FT /note="OEF80" FT /db_xref="GOA:Q59312" FT /db_xref="HSSP:1KWG" FT /db_xref="InterPro:IPR003476" FT /db_xref="InterPro:IPR013529" FT /db_xref="InterPro:IPR013738" FT /db_xref="InterPro:IPR013739" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/TrEMBL:Q59312" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="BAA08485.1" FT /translation="MLHGADYNPEQWIDMPNIWGEDVRLMKLSHTNVVAVGIFSWTMLE FT PEEGKFNFEWLDEIMDLMHKNGNYVILATPSGAKPIWMAHKYPETLRVAQNRVRNLYGE FT RHNHCYTSPIYREKIAIIDRLLAERYKDHPALILWHISNEFEGQCYCPLCEEAFRDFLR FT EKYDNDINKLNKAWWTKFWSHTYASFDEIEAPAPHGEPALHGLNLDWMRFVTHQTLDYY FT KHERSILKEITPDIPVTTNFHDYISLFRGIDYWKFAPYLDVVSWDNYPYWHGERTDDHE FT GSRIGFVHDLNRAILNGKPFMMMESSPSSTNWQPVAKLRRPGMHVLSSLQAVAHGSDTV FT QYFQWRKSRGSSEKFHGAVVDHCGHENTRVFRDVTKVGEILSKLDDVIGTSVEPQVAVI FT YDWENYWAINDAQGPRIEQKDYFETCQKHYKAFWDMSIPTDVINMDCDFSKYKVVVAPM FT LYMVRPGVGERLEEFVKNGGTLVTTYWSGIVDENDLCFLGGFPGPLKKVTGIWAEELDA FT LYDEDVNYVSVEEGNSLGMKGEYEARIFCDLIHSEGAEVLATYKTDFYKGMPALTCNNF FT GEGQAYYIAFRNNDEFLSDFYSSLAKKLTLKRAIEIDLPKGINAQVRMDEKNEFVFFMN FT FSSEEKTIDIKDLDLTDMVTGEKVAKEMEIEPYGVRIVRRK" XX SQ Sequence 5531 BP; 2093 A; 651 C; 967 G; 1820 T; 0 other; tataccctgg atctagtatt acttacaatt aaactaaagt aaaaactgat tttagctagt 60 ctaaaatcag ttttttattt catattttag agttataata aagtaaatat tagatttgtt 120 tggacattac tatacataaa tttggtttac atgtattaga ttcatatgaa ttaaaaagtg 180 tttatgaagc cgagtttaat aaaattttta ttggaggaat taaaatgaaa ttagctgagg 240 cattgatttt aagagcagat ttacaaaaag aattaatgag tttaaaaaat agaataaatc 300 aaaacttact agttcaagaa ggtgagaaac cctctgagaa cccatataca ctatatgatg 360 agatactgtt aaaagtaagt gaattagagg ggttaattct aaagattaat aaaactaata 420 gtaattacat aactagtaat aatgaaagta tttctgattt gattgtaaaa agagatatgc 480 ttggaaagaa gataagcata ttaaaagaaa tagctgaaga agctacagta aaagtaaata 540 gatactctca aactgaaata aaaatatttt ctgcaatgaa ggtagatcaa ttacagagag 600 aaataaataa atattctaag gaatatagag aactagacac taaactgcaa ggtttaaact 660 ggacaataga tttaatggaa taaaagcatc taaattataa agttaattat tcctagttta 720 aactagataa tagattaatt aaatagaggt ttaaatgttg gtagctttta ttaaggaggt 780 cataaatcct aacctgggga aaaaggtgat tatacatgta tctaggagca gatacttatt 840 ttataattta tgctcaaaca agttacatgg gtactattta agaattacaa tttacaacca 900 tatgactatt aataataagc gaggatgggg taaggggata caatttaaag aagatagagt 960 tatgattcat taaggaaatt atagctctat tttttaattt taaattacaa agttatttta 1020 attaaagtaa ataaaagcta attaaagtta atacatatct ttttatataa ttctattcta 1080 aaaatttaat tttaacttat tttagcttac taataatttc taaaattaaa tgtagaaaaa 1140 tactttttaa agaattttct tctttagtta tagcaaatac ttaataagta aaaattttag 1200 tgatcgttta atgggctata cttctacata aactcttcaa aaacaattaa ttataaagtt 1260 tcttcaaaat tattttccaa taaaatcaaa aaaatataaa ttataataaa ttattaagga 1320 taataataaa taaaaaacta aatttcaata taattcacag aaaatcaagt ttaaccccta 1380 ataaataata aagagtaaac tttaactaaa aaacttaaag aaataaaaga ggtggttgca 1440 tttgattatt aataagttaa tttatttaga aaataatgat aaaatattaa aaaataacaa 1500 tatttttatg tgatttatta ataaatagag ctttttataa atattatgca taatacttaa 1560 tgttaaaaat attttaacta aggatgattt agtaaaataa tttagtaaaa ttatagtttt 1620 tttactaaat attcaaattt tgtacattaa catttctaca aaaaatgata ttataatgtc 1680 agaaaacgtt tcctaaggaa actttatact taaaatatga aatatgaggg ggatttttaa 1740 gatgagtgag agaaaaaggt cgcttagttc aggggcttta atgcttatga catttacggc 1800 ggtattttca ttcggaaaca taattgatag tagtgtaaat attggactag ctacaatacc 1860 atcatacata tttggtacag tattttactt tttaccattt gctttgatga ttggtgagtt 1920 tgcttcagct agttcagatt ctgaatcagg tataaatagt tggataaaga aatctttagg 1980 agcaagatgg gctttcttag gatcatggtc ttatttcttt gttaacttgt tcttctttac 2040 ttcattatta cctaaaatat taatttatgc atcttatacc tttgttggta gaaacgtatt 2100 tgatggaaaa acagtattaa tttctgttat atcaatagta ttattctggg cagtaactat 2160 aatttctact aagggtgtat cttggatttc aaaaataaca agtatatctg gtgtagctag 2220 aataatttta ggtttaggat tcatagtatt atctttcgga gttattttat tcttaggaaa 2280 agctccagct caagaattca cagcagaaac tattatgcct aagtttaact ggtcatattt 2340 catggtttta gcttggattt tacaagcagt aggtggtgct gaaagtatag gtgtatatat 2400 taaagacgta aagggtggaa ataaaacatt cataagaaca atggtaattt caactgctat 2460 agttggtggt ctatatgcac ttggagctgt ttcagtaggt ttagttgtac cttcagaagt 2520 attacaaggt aatttctcaa atggtttatt tgatgccttt gcaatattag gtgcaaacta 2580 tggagtaggt aacataataa ctaacatagt tggattcata atgatgcttg cttctttagg 2640 ttcattagtt ttatggactg cagcacctgt taaagtatta ttctctgaaa ttcctgaagg 2700 aatctttggt aaatggattg ctaaaacaga taaaaaagga actcctgtaa atgctttata 2760 tgtacaagct gtaatagtaa cagtattact attagtacca gctttaggaa taggttcagt 2820 tgatagctta cttgaaatgt taataaacat gactgcttca acttcattaa ttccagtatt 2880 attcttctta gttggataca ttgtattaag agctaagaaa gaccatatgg aaagatcatt 2940 taaagttgga tctaaaaact ttggaatagc aattggagta ctattacttg ctttattcgt 3000 attcgtattt gtaatatctt caattccagc tccacaagac tttgcagctt actttaatgg 3060 aacattagca gaaggagcaa caaatcctgt atttatactt ttatacaatg tattaggatt 3120 agtattcttc ttaggttttg ctgaaatatg ctggagaaaa tatgaaaaga aagttggaaa 3180 agctgtagct aatgaatggg atcaagaaga ggtttcagaa atagcttaat taaagtaatg 3240 acaaaattat aaaagatatc aattaaggaa taagctataa aaatatagct tattcctata 3300 actataaatt tatttagaaa tacacaattt gctttataga ttaataagag aaaaaaatga 3360 ttaacttggt aagatggagg gattattttg aaaaattatg gaccaataag ttctaaagta 3420 acaaaaatgc ttcatggagc agattacaac ccagagcaat ggattgatat gcctaacata 3480 tggggagaag atgttagatt aatgaagctt tctcatacaa atgttgtagc tgtaggcata 3540 ttctcatgga caatgttaga gccagaggaa ggtaagttta actttgaatg gttagatgaa 3600 atcatggatt taatgcacaa gaatggaaac tatgtaatac ttgctacacc aagtggagct 3660 aagccaatat ggatggctca taagtatcca gaaactttaa gagttgctca aaacagagta 3720 agaaacttat atggagagcg tcataaccat tgctatacat ctcctatata tagagagaaa 3780 attgctataa tagacagatt attagctgaa agatataaag atcacccagc tttaatttta 3840 tggcatattt caaatgagtt tgaaggtcaa tgttactgtc ctttatgtga agaagctttt 3900 agagatttct taagagagaa atatgataat gatataaata aattaaataa agcttggtgg 3960 actaagttct ggagccatac atatgcttcc tttgatgaaa tagaagcgcc agctcctcat 4020 ggagaaccag ctcttcatgg attaaactta gactggatga gatttgttac tcaccaaact 4080 ttagattatt acaagcatga aagaagcata ttaaaggaaa tcacaccaga tattcctgta 4140 actactaact tccatgacta tatttcatta tttagaggaa tagattactg gaagtttgct 4200 ccttacttag atgttgtatc atgggataac tatccttact ggcatggtga aagaacagat 4260 gaccacgaag gaagtagaat tggatttgtt catgacttaa atagagcaat tttaaatggt 4320 aagccattta tgatgatgga aagttctcca agttcaacaa actggcaacc agttgcaaaa 4380 cttagacgtc caggaatgca tgttttatct tctcttcaag ctgtagcaca tggttcagat 4440 acagttcaat acttccaatg gagaaagagt agaggatcat cagaaaagtt ccatggagct 4500 gttgttgatc actgtggaca tgaaaataca agagtattta gagatgtaac taaggttgga 4560 gaaattctat caaaacttga tgatgtaata ggaacttctg tagaaccaca agtagctgtt 4620 atttacgatt gggaaaacta ctgggcaata aatgatgctc aaggtcctag aatagagcaa 4680 aaggattact ttgaaacttg tcaaaagcac tacaaagctt tctgggatat gtcaattcca 4740 acagatgtta taaacatgga ttgcgatttc tcaaaatata aggttgttgt agcaccaatg 4800 ctttatatgg taagaccagg tgttggagaa agattagagg aattcgttaa aaatggagga 4860 actttagtaa caacttactg gagtggtatt gttgatgaaa atgacctttg cttcttagga 4920 ggattccctg gcccattaaa gaaagttaca ggaatctggg cagaagaatt agatgcactt 4980 tatgatgaag atgtaaacta tgtttctgtt gaagaaggca atagcttagg aatgaaaggt 5040 gaatatgagg ctagaatatt ctgtgactta attcactcag aaggagctga agttcttgca 5100 acatacaaaa cagacttcta caaaggaatg ccagctttaa cttgcaacaa ctttggagaa 5160 ggacaagctt actatatagc atttagaaat aatgatgagt tcttatcaga tttctactca 5220 agcttagcta agaagttaac tttaaagaga gctatagaaa ttgacttacc aaagggaatt 5280 aatgctcaag ttcgtatgga tgaaaagaac gagtttgtat tctttatgaa cttctcatca 5340 gaggaaaaaa caatagacat taaagatctt gatttaacag atatggtaac tggtgaaaag 5400 gtagctaagg aaatggaaat agagccttat ggagttagaa tagttagaag aaaataaaat 5460 agatattaat atataaataa gatttgtatt tagtttaata gattaataag aaaaaagtaa 5520 tgctgaatac t 5531 // |
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