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EBI DbfetchID D37969; SV 1; linear; genomic DNA; STD; PRO; 3630 BP. XX AC D37969; XX DT 29-JUN-1995 (Rel. 44, Created) DT 11-AUG-2007 (Rel. 92, Last updated, Version 9) XX DE Pimelobacter simplex ksdR, ksdD, ksdI genes for hypothetic regulatory DE protein, 3-ketosteroid-1-dehydrogenase, 3-ketosteroid-5-isomerase, complete DE cds. XX KW . XX OS Pimelobacter simplex OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Propionibacterineae; Nocardioidaceae; Pimelobacter. XX RN [1] RP 1-3630 RA Murooka Y.; RT ; RL Submitted (15-AUG-1994) to the EMBL/GenBank/DDBJ databases. RL Yoshikatsu Murooka, Faculty of Engineering, Hiroshima University, RL Department of Fermentation Technology; 1-4-1 Kagamiyama, Higashi-Hiroshima, RL Hiroshima 724, Japan (E-mail:murooka@ue.ipc.hiroshima-u.ac.jp, RL Tel:81-6-879-7416, Fax:81-6-879-7418) XX RN [2] RX DOI; 10.1111/j.1365-2958.1995.tb02359.x RX PUBMED; 7596291. RA Molnar I., Choi K., Yamashita M., Murooka Y.; RT "Molecular cloning, expression in Streptomyces lividans, and analysis of a RT gene cluster from Arthrobacter simplex encoding 3-ketosteroid-delta RT 1-dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical RT regulatory protein"; RL Mol. Microbiol. 15(5):895-905(1995). XX FH Key Location/Qualifiers FH FT source 1..3630 FT /organism="Pimelobacter simplex" FT /strain="IFO 12096" FT /mol_type="genomic DNA" FT /note="synonym: Arthrobacter simplex" FT /db_xref="taxon:2045" FT RBS 481..485 FT CDS 493..1311 FT /codon_start=1 FT /transl_table=11 FT /gene="ksdR" FT /product="hypothetic regulatory protein" FT /db_xref="GOA:P70766" FT /db_xref="InterPro:IPR005471" FT /db_xref="UniProtKB/TrEMBL:P70766" FT /protein_id="BAA07185.1" FT /translation="MTVTALPTTTPAGSGAPALDPDDRRTPLGVVGRVTRILNAFSESP FT DRLMLEDVMALTGLPRSTAFRILGQLIDEGWVEHDTRGYRLGPHAPTLTGRPGEHQEVR FT VAASPYLNELHALTGAVAHLSVLEGDRVHYLDKIGGSAARAVPSRVGARLLASDTVSGR FT ALLACRSPEYVDDVLGPRLPAPRLALLHRDLAAARQRRGVVHAPADPTTGIASIAAPVL FT GPHGAVAAISLALPGELPPARLAPLLLNQAHRIAGVLFPQRRLHGRSWLR" FT RBS 1423..1429 FT CDS 1435..2982 FT /codon_start=1 FT /transl_table=11 FT /gene="ksdD" FT /product="3-ketosteroid-1-dehydrogenase" FT /EC_number="1.3.99.4" FT /db_xref="GOA:P77815" FT /db_xref="HSSP:1D4D" FT /db_xref="InterPro:IPR005829" FT /db_xref="UniProtKB/TrEMBL:P77815" FT /protein_id="BAA07186.1" FT /translation="MDWAEEYDVLVAGSGAGGMAGTYTAAREGLSVCLVEAGDKFGGTT FT AYSGGGGAWFPANPVLLRAGTDDTIEDALEYYRAVVGDRTPADLQETYVRGGAGLVAYL FT EEDDHFSFESYPWPDYFGDAPKARRDGQRHIIPTPLPVPSAPELREVVRGPLDNDRLGT FT PQPDDLFIGGRALVARFLTALATYPHATLVRETALAELVVEDGVVVGAIVETDGVRRAI FT RARRGVLLAAGGFEANDELRQKYGVPGVARDTMGPPTNVGAAHQAAIAVGADTDLMGEA FT WWSPGLTHPDGRSAFALWFTGGIFVDGAGRRFVNESAPYDRLGRAVIDHLTEGGVTPRY FT WMVYDHKEGSIPPVRATNVSMVDEEQYVAAGLWHTADTLPELAALIGVPADALVATVAR FT FNELVADGYDADFGRGGEAYDRFFSGGEPPLVSIDEGPFHAAAFGISDLGTKGGLRTDT FT SARVLTADGTPIGGLYAAGNTMAAPSGTTYPGGGNPIGTSMLFSHLAVRHMGTEDAR" FT CDS 2979..3350 FT /codon_start=1 FT /transl_table=11 FT /gene="ksdI" FT /product="3-ketosteroid-5-isomerase" FT /EC_number="5.3.3.1" FT /db_xref="GOA:P77816" FT /db_xref="HSSP:1OPY" FT /db_xref="InterPro:IPR002075" FT /db_xref="UniProtKB/Swiss-Prot:P77816" FT /protein_id="BAA07187.1" FT /translation="MSAEVKAAVARYLDAVAGGSPAAIAALYAPDATLEDPVGADLVRG FT RAAIEEFYGALAGAKVSTELLAVRAVAGHAAFSFRVTTDAGDQQYVVEPIDVMTFDADG FT QITSMRAFWAPGDMVVTPA" XX SQ Sequence 3630 BP; 475 A; 1436 C; 1243 G; 476 T; 0 other; ctgcaggagc tcggcctggt cgagcgggcc gcggacacct tcgaccggcg caccacgctg 60 gtccgctgct cgcgccgcgg cgtcgcccag gtacgccggc tcgcggccgc ccagcgcgcc 120 gacctagccg ccgcgctcgg tccggtcgac ccggccgacc gggaccgctg gacggtgctc 180 gtggagcgct acgtgcgggc tctcgaggcc cgcgggctca tctccgagct gtgactcgcc 240 ggtaagttca gagaacatta tgtgcaaacg gtccagtaaa actagccgtt cggcaagtag 300 attggtgacc catcgcattc tgtgtttccg caggtcagag gcacagtttc ggaggtgacc 360 gcagtcccgg tgaccgggag tgccgattca cggcggaaac ctcaccgaaa aatatgtgcg 420 ttcgatccac ttgatttgcc ctgtgtcagt gctcacactc gacgggaggc cgcactcccg 480 aggagcaccc gcatgaccgt caccgcactg cccacgacca cgcccgccgg ctccggcgca 540 cccgccctgg accccgacga ccgccgcacg cccctgggcg tcgtgggccg ggtgacccgg 600 atcctcaacg ccttcagcga gtcccccgac cgcctcatgc tcgaggacgt gatggcgctg 660 accggcctgc cccggtcgac cgccttccgg atcctcggcc agctcatcga cgaggggtgg 720 gtcgagcacg acacccgcgg ctaccggctc gggccgcacg cgcccacgct caccggccgg 780 cccggcgagc accaggaggt gcgggtcgcc gcgtcgccgt acctcaacga gctgcacgcc 840 ctcaccggcg cggtcgccca cctctcggtg ctcgagggcg accgggtcca ctacctcgac 900 aagatcggcg gctccgcggc tcgcgccgtc ccctcgcggg tcggcgcccg gctgctcgcc 960 tccgacaccg tcagcggccg cgcgctgctc gcctgccgct cccccgagta cgtcgacgac 1020 gtcctcggcc cgcggctgcc cgcgccccgg ctcgccctgc tccaccgcga cctcgccgcc 1080 gcccgccagc gccgcggcgt cgtgcacgcc ccggccgacc cgaccaccgg catcgcctcg 1140 atcgccgcac ccgtcctcgg cccgcacgga gccgtcgccg cgatctcgct ggccctgccc 1200 ggcgagctgc cgcccgcccg gctcgcaccc ctgctgctca accaggccca ccggatcgcc 1260 ggcgtcctgt tcccccagcg ccgcctgcac ggacgatcct ggctgcgctg atcccgcccc 1320 cgcccggaga ctcccgcagg acgggagaac ccaccggggc acccggggcc gctgcctagc 1380 gtcgccgcca cgacgccgga ggtcggcgtc ggtcaacccg gcgagaggat cacgatggac 1440 tgggcagagg agtacgacgt actggtggcg ggctccggcg ccggcggcat ggccgggacc 1500 tacaccgcgg cccgcgaggg gctcagcgtg tgcctggtcg aggccgggga caagttcggc 1560 gggacgaccg cctactccgg cggcggtggg gcctggttcc ccgcgaaccc ggtgctgctg 1620 cgggcgggca ccgacgacac gatcgaggac gctctcgagt actaccgagc ggtcgtcggc 1680 gaccgcaccc ccgcggacct gcaggagacc tacgtccgcg gcggcgccgg cctggtcgcc 1740 tacctcgagg aggacgacca cttctccttc gagtcctacc cgtggccgga ctacttcggc 1800 gacgccccca aggcccgtcg cgacggccag cggcacatca tcccgacgcc gctgccggtg 1860 ccctccgcac ccgagctgcg cgaggtggtc cgcgggccgc tcgacaacga ccggctcggc 1920 acgccgcagc ccgacgacct gttcatcggc ggacgggcgc tcgtcgcccg cttcctgacc 1980 gcgctcgcga cctaccccca cgccacgctc gtgcgcgaga ccgcactggc cgagctcgtc 2040 gtcgaggacg gcgtcgtggt cggcgcgatc gtcgagaccg acggcgtccg ccgcgcgatc 2100 cgggcccgcc gcggcgtcct cctggccgcg ggcggcttcg aggccaatga cgagctccgc 2160 cagaagtacg gcgtccccgg cgtcgcgcgc gacacgatgg gcccgccgac caacgtcggc 2220 gccgcgcacc aggccgcgat cgcggtcggc gccgacaccg acctgatggg cgaggcctgg 2280 tggtcccccg ggctgaccca ccccgacgga cgatcggcgt tcgcgctctg gttcaccggc 2340 ggcatcttcg tcgacggcgc cggccggcgc ttcgtcaacg agtcggcgcc gtacgaccgg 2400 ctcggccgcg ccgtcatcga ccacctcacc gagggcggcg tcaccccgcg gtactggatg 2460 gtctacgacc acaaggaggg ctcgatcccc ccggtgcgcg ccaccaacgt ctcgatggtc 2520 gacgaggagc agtacgtcgc cgcgggcctg tggcacaccg ccgacacgct gcccgagctg 2580 gccgcgctga tcggcgtccc cgccgacgcg ctggtcgcca cggtcgcgcg cttcaacgag 2640 ctcgtcgccg acgggtacga cgcggacttc ggccgcggcg gcgaggccta cgaccggttc 2700 ttctccggcg gcgagccgcc gctggtgagc atcgacgagg ggccgttcca cgcggccgcc 2760 ttcggcatct ccgacctcgg caccaagggc gggctgcgca ccgacacgtc cgcgcgcgtg 2820 ctgaccgcgg acggcacgcc gatcgggggc ctctacgcag ccggcaatac gatggcggcg 2880 ccgagcggca ccacctaccc gggcggtggc aacccgatcg ggacaagcat gctcttcagc 2940 cacctcgccg tgcggcacat gggcaccgag gacgcgcgat gagcgccgag gtgaaggccg 3000 ccgtggcgcg ctacctcgat gctgtcgccg gcggctcgcc ggccgcgatc gccgcgctct 3060 acgcccccga cgccacgctc gaggaccccg tcggcgccga cctcgtccgc ggccgcgcgg 3120 cgatcgaaga gttctacggc gccctcgccg gcgcgaaggt cagcaccgag ctgctcgccg 3180 tccgcgccgt cgcgggccac gccgcgttct cgttccgggt caccaccgac gccggcgacc 3240 agcagtacgt cgtcgagccg atcgacgtga tgacgttcga cgcggacggc cagatcacgt 3300 ccatgcgggc gttctgggcg cccggggaca tggtcgtcac gccggcctga cggtcccgct 3360 gtaacacgct gtccaccgcg cttcccggcg gttgtcgacg cgctctcggc gtgtcgcacg 3420 gcgtgtcgcg ccgtggacag cgtgttacag cggcgggggc cgtcaggcgg tggccgcgtg 3480 ggtggcgacg atgtggccga agaccagacc ctggccgatg gtcgcgccgg cccccgggta 3540 gctgcgcccg aagacgttgc ccgcggtgtt gccgatcgcg tagagcccct cgatcgggct 3600 gccgtcggcg cgcagcggac ggccgagctc 3630 // |
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