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EBI DbfetchID D37964; SV 1; linear; genomic DNA; STD; PRO; 1769 BP. XX AC D37964; XX DT 18-AUG-1994 (Rel. 40, Created) DT 01-FEB-2000 (Rel. 62, Last updated, Version 7) XX DE Bacillus subtilis gene for beta-mannanase, complete cds. XX KW beta-mannanase; beta-mannosidase. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-1769 RA Arai M.; RT ; RL Submitted (15-AUG-1994) to the EMBL/GenBank/DDBJ databases. RL Motoo Arai, Osaka Prefecture University, Department of Applied Biological RL Chemistry; Gakuen-cho 1-1, Sakai, Osaka 599, JAPAN RL (E-mail:motoo@main.biochem.osakafu-u.ac.jp, Tel:0722-52-1161(ex.2476), RL Fax:0722-52-0341) XX RN [2] RP 1-1769 RX DOI; 10.1016/0304-4165(95)00011-Y RX PUBMED; 7727534. RA Mendoza N.S., Arai M., Sugimoto K., Ueda M., Kawaguchi T., Joson L.M.; RT "Cloning and sequencing of beta-mannanase gene from Bacillus subtilis RT NM-39"; RL Biochim. Biophys. Acta 1243(3):552-554(1995). XX FH Key Location/Qualifiers FH FT source 1..1769 FT /organism="Bacillus subtilis" FT /isolate="NM-39" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT -35_signal 322..327 FT -10_signal 347..352 FT RBS 479..484 FT CDS 493..1575 FT /codon_start=1 FT /transl_table=11 FT /product="beta-mannanase" FT /EC_number="3.2.1.25" FT /db_xref="GOA:P55278" FT /db_xref="InterPro:IPR016714" FT /db_xref="UniProtKB/Swiss-Prot:P55278" FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="BAA07178.1" FT /translation="MLKKLAVCLSIVLLLLGAASPISAHTVYPVNPNAQQTTKDIMNWL FT AHLPNRSENRVMSGAFGGYSDVTFSMTEENRLKNATGQSPAIYGCDYGRGWLETADITD FT TIDYCCNSSLISYWKSGGLPQVSLHLANPAFPSGNYKTAISNSQYKNILDPSTVEGKRL FT EALLSKIADGLTQLKNQGVTVLFRPLHEMNGEWFWWGLTGYNQKDNERISLYKELYKKI FT YRYMTETRGLDNLLWVYSPDANRDFKTDFYPGSSYVDITGLDAYFTDPYAISGYDEMLS FT LKKPFAFAETGPSGNIGSFDYAAFINAIRQKYPQTTYFLTWDEQLSPAANQGAQSLYQN FT SWTLNKGEIWNGGSLTPIAE" FT sig_peptide 493..564 FT mat_peptide 565..1572 FT /product="beta-mannanase" FT /EC_number="3.2.1.25" FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT terminator 1583..1605 XX SQ Sequence 1769 BP; 483 A; 425 C; 412 G; 449 T; 0 other; gcatgcaagc ttgcatgctt acctgccagt ttgtattttt tcagtgaggc gatcagtttt 60 tcttccatac ccggatatgt ttcaggcgcc tttgtctcaa tataatagtt tgcatgtctg 120 ccgaaccgtt ttaacacgtc atcaagtgtg ggaacgcgaa gtccggcgta gtgcggtttt 180 gctttttccg gataggcctt attgaaccaa gaaccggcgt cgagcttttg aatctcggct 240 aatgtatgat ccttcaccca gccttgtccg tttgtggtgc ggttcagctt ttcatcatgc 300 atgacgatca gcttgccgtc ttttgtcatt tgtaaatcca gctcaatata gtcagccttc 360 attttttgcg cagtttcata agaaaggatc actttatcgt gccggcggat tccggagatt 420 ttgttataga aggagattgt gaggttatcg tttcacatcc ttcatcatct taaaaatcag 480 gaggtctttt atatgcttaa aaagttagca gtctgcctgt ctatcgtttt attactctta 540 ggagccgcca gtccgatatc ggctcacacc gtttatcccg tcaacccaaa tgcccagcag 600 acgacaaaag atatcatgaa ctggctggcc cacctgccca accgttcaga aaacagggtc 660 atgtccggag cgttcggcgg gtacagcgat gtcacttttt caatgacaga ggaaaaccgc 720 ttgaaaaacg cgacgggaca gtctcccgcc atctacggct gtgactatgg gagaggatgg 780 ctggaaacag cggatatcac cgatactatc gattactgct gcaacagcag cttaatctca 840 tactggaaaa gcggcggcct ccctcaggtc agcctgcatc tcgcaaatcc ggcctttcca 900 tccggaaact ataaaacggc catctcaaac agccagtaca aaaacatcct tgacccttca 960 actgtggaag gaaaacggct tgaggcgctg ctcagcaaaa ttgccgacgg ccttactcag 1020 ctgaaaaatc aaggcgtcac cgttctgttc agaccgctgc atgaaatgaa cggcgagtgg 1080 ttctggtggg ggctgacagg ctacaaccaa aaagacaatg agagaatctc gctgtacaaa 1140 gagctttaca agaaaatata ccgctatatg acagaaacaa gaggattgga taaccttttg 1200 tgggtgtatt cgccggatgc caacagagac tttaaaacag acttctaccc aggctcatct 1260 tatgtagata ttaccggtct ggacgcttac ttcactgatc cgtatgcgat atcaggctat 1320 gatgaaatgc tgtctctgaa aaaaccgttt gcctttgccg aaaccggtcc gtccggcaat 1380 atcggaagct ttgattatgc tgcttttatt aatgcgatca ggcaaaaata ccctcagacc 1440 acgtactttt tgacatggga tgaacaatta agtccggcgg ccaatcaagg cgcgcaaagc 1500 ctttatcaaa acagctggac gctgaacaag ggcgaaatat ggaacggcgg gtccttgacg 1560 ccgatcgcgg aataaccgct tcttttgaaa atcggtttta aaaaacggct ctgctgggca 1620 tattgttacc gttcaaaaag gagggttcaa caatggaaaa taacaaaaac agcgaagaaa 1680 cgctttccag taagacgacc ggtaaggcga gtcagaagac acgctgacaa accggcgggc 1740 catccggtca ctgacaacca gaatgtcag 1769 // |
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