 |
|
EBI DbfetchID D32121; SV 1; linear; genomic DNA; STD; PRO; 2160 BP. XX AC D32121; XX DT 15-JUL-1994 (Rel. 40, Created) DT 28-MAY-1999 (Rel. 59, Last updated, Version 4) XX DE Pseudomonas marginalis gene for pectin lyase, complete cds. XX KW pectin lyase. XX OS Pseudomonas marginalis OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 1-2160 RA Nikaidou N.; RT ; RL Submitted (07-JUL-1994) to the EMBL/GenBank/DDBJ databases. RL Naoki Nikaidou, Niigata University, Department of Appl. Biol. Chem.; 8050 RL Ikarashi-2, Niigata, Niigata 950-21, Japan (Tel:025-262-6645, RL Fax:025-263-1659) XX RN [2] RX DOI; 10.1016/S0006-291X(05)80105-6 RX PUBMED; 1731776. RA Nikaidou N., Kamio Y., Izaki K.; RT "Molecular cloning and nucleotide sequence of a pectin lyase gene from RT Pseudomonas marginalis N6301"; RL Biochem. Biophys. Res. Commun. 182(1):14-19(1992). XX FH Key Location/Qualifiers FH FT source 1..2160 FT /organism="Pseudomonas marginalis" FT /strain="N6301" FT /mol_type="genomic DNA" FT /clone="pPNL6301" FT /db_xref="taxon:298" FT -35_signal 347..352 FT -10_signal 370..375 FT /inference="non-experimental evidence, no additional FT details recorded" FT protein_bind 372..389 FT /standard_name="LexA binding site" FT /function="repressor of SOS response" FT /bound_moiety="LexA" FT /inference="non-experimental evidence, no additional FT details recorded" FT -35_signal 457..462 FT -10_signal 479..484 FT RBS 506..511 FT CDS 515..1453 FT /codon_start=1 FT /transl_table=11 FT /standard_name="pectinase" FT /product="pectin lyase" FT /EC_number="4.2.2.10" FT /db_xref="GOA:P27027" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/Swiss-Prot:P27027" FT /protein_id="BAA06847.1" FT /translation="MSYPESKLTGLTGFALAAKVTGGWAGPVVSITNLDQLKANIGTVT FT PQVLVINSNISASSLTKVNMGANKTLIGSFQNRTLENIHLRATAQSQNIILQNLIFKHS FT ANIKANDDIQVYLNYGSKYWIDHCSFVGHSWSTTDGSEDKLLYIGEKADYATISNCFFG FT SHKYGLIFGHPADDNNAAFNGYPRLTLCHNRFDNMEVRAPGLMRYGYFHVYNNYINKFH FT LGFTLAQNANILSESNYFGEGSQNNGMLDDKGSGTFTDTNSVPPITNQKSPKAQWTATS FT NYAYTLKTAAQAKDFTQKNAGAQAAALVFGS" FT attenuator 1493..1525 XX SQ Sequence 2160 BP; 528 A; 569 C; 565 G; 498 T; 0 other; aattcggcaa cggcgtcagc tgcttcaccc gcgacggcaa catcgcccgc gagttcgcac 60 gccgcatcca agtaggcatg gtcggcatca acgtaccgat cccggtgccg atggcctggc 120 acgggtttgg cggctggaag aagagcctgt ttggcgacat gcatgcctac ggcaccgagg 180 gtgtgcgctt ctacaccaag cagaagtcga tcatgcagcg ctggtcggag agcatcgagc 240 agggcgcgga gtttgcgatg cctgtgtcca gataacgctt gaaaggacac cgtaggacaa 300 gtgtgggagg gagcaagctc cctcccacat tttttttgcg taaaggttga cgatccagtg 360 aatgccgaat actgtatata aacacagtgt ttaggcgctg accaaccaag ctctggcagc 420 ctcagccacg ccaaacaccc tgtgtaagtt aatggattaa cttaatgctc aggctcgtta 480 taccgagccg tttttataga gagagaggga actcatgtcg tacccagaaa gcaaactcac 540 cggtttgacc ggtttcgccc tcgcggcaaa agtgaccggc ggctgggccg gccccgtggt 600 cagcatcact aacctggatc aactcaaggc caatatcggc accgtcaccc cgcaggtgct 660 ggtgatcaac agtaatatct cggcgtcgag cctgaccaaa gtcaacatgg gcgccaacaa 720 gacgctgatc ggttcgttcc agaatcggac gttggagaat attcacctgc gtgcaaccgc 780 gcagtcgcag aacatcattc tgcagaatct tatcttcaag cattcagcga atatcaaggc 840 caatgacgat attcaggtct acctgaacta cggcagcaag tattggattg accattgctc 900 ttttgtcggg cacagctggt cgaccactga cggcagtgaa gacaaattgc tctatatcgg 960 tgaaaaagcc gattatgcca ccatcagcaa ctgcttcttt ggcagtcaca agtacggcct 1020 gatcttcggt catccggcgg atgataataa cgcggcgttc aacggctacc cgcgcctgac 1080 actgtgccat aaccgcttcg acaatatgga agtgcgtgcg cctggcttga tgcgctacgg 1140 ttattttcat gtgtataaca actatatcaa taagtttcac ctcggtttca cccttgcgca 1200 gaatgccaat atcctttccg agagcaacta ttttggtgaa ggcagccaaa acaatggcat 1260 gttggatgat aaaggcagcg ggacgtttac tgatacaaac agtgtgccgc cgatcaccaa 1320 ccagaagtcg ccaaaagcac agtggacggc aacttccaat tacgcctata ccttgaagac 1380 ggcggcgcag gccaaggact ttacacagaa gaatgccggg gctcaggctg cggcgttggt 1440 gtttggtagt tgaatagcta gctttctact tggctgtcat tattgcaggt gccactgtat 1500 ttatatcaag gatataaata cagtgatggc ctttgtaatg taaacaattc ttcatgttga 1560 gataagcggt gcccatgtta aaccttgggg tgcacttgca gatccaacta tcttgtgctc 1620 tttcggtcag tatcgagttg ctggctgtca ggtgctattt aatcttcgct ttttatttaa 1680 gcgtcagcaa aaggacatca tcatgaaaca tgcgaatcat actcccactc gctggacacg 1740 ggccgatgcg ctgaaagtta atgagaatga ccctacgacg acacagccgt tagtgagtcc 1800 tgactttcct gtcatgagtg acacggtctt tatttgggac accatgccat tgcgtgagct 1860 ggatggcacg gtggtatctg tggatggctg gtcagtgatc ttcaccctga ccgccgaccg 1920 tcgcccccat gatccacaat tcatcaacgc cgacggccgt tacgacatca agcgcgattg 1980 ggaagatcgc catggccatg cgcggatctg ctactggtac tcgcgcaccg gcaaagactg 2040 gatcttcggc ggccgggtca tggccgaagg ggtttcaccg accacgcgtg agtgggccgg 2100 cacgccgatc ctgctcaata acaacggtga tatcgacctg tattacacct gcgtaacccc 2160 // |
|