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EBI DbfetchID D31821; SV 1; linear; genomic DNA; STD; PRO; 2749 BP. XX AC D31821; XX DT 27-JUL-1994 (Rel. 40, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 5) XX DE Streptomyces thermotolerans carB, acyB1 or carE, and acyB2 genes for DE carbomycin resistance detarminant, 4'-mycarosyl isovaleryl-CoA transferase DE and regulatory protein, partial and complete cds. XX KW 4'-mycarosyl isovaleryl-CoA transferase; carbomycin resistance determinant; KW regulatory protein. XX OS Streptomyces thermotolerans OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptomycineae; Streptomycetaceae; Streptomyces. XX RN [1] RP 1-2749 RA Arisawa A.; RT ; RL Submitted (10-JUN-1994) to the EMBL/GenBank/DDBJ databases. RL Akira Arisawa, Mercian Corporation Central Laboratories; 4-9-1 Johnan, RL Fujisawa-shi, Kanagawa 251, Japan RL (E-mail:mercian2@city.fujisawa.kanagawa.jp, Tel:0466-35-1511, RL Fax:0466-35-1530) XX RN [2] RX DOI; 10.1016/0378-1119(87)90094-1 RX PUBMED; 3036668. RA Epp J.K., Burgett S.G., Schoner B.E.; RT "Cloning and nucleotide sequence of a carbomycin-resistance gene from RT Streptomyces thermotolerans"; RL Gene 53(1):73-83(1987). XX RN [3] RX DOI; 10.1016/0378-1119(89)90421-6 RX PUBMED; 2628170. RA Epp J.K., Huber M.L., Turner J.R., Goodson T., Schoner B.E.; RT "Production of a hybrid macrolide antibiotic in Streptomyces ambofaciens RT and Streptomyces lividans by introduction of a cloned carbomycin RT biosynthetic gene from Streptomyces thermotolerans"; RL Gene 85(2):293-301(1989). XX RN [4] RP 1-2749 RX PUBMED; 7764361. RA Arisawa A., Kawamura N., Tsunekawa H., Okamura K., Tone H., Okamoto R.; RT "Cloning and nucleotide sequences of two genes involved in the RT 4''-O-acylation of macrolide antibiotics from Streptomyces thermotolerans"; RL Biosci. Biotechnol. Biochem. 57(12):2020-2025(1993). XX FH Key Location/Qualifiers FH FT source 1..2749 FT /organism="Streptomyces thermotolerans" FT /strain="TH475" FT /mol_type="genomic DNA" FT /clone="pMAB60" FT /db_xref="taxon:80858" FT CDS <1..34 FT /codon_start=2 FT /transl_table=11 FT /gene="carB" FT /product="carbomycin resistance determinant" FT /db_xref="UniProtKB/TrEMBL:P74843" FT /citation=[2] FT /protein_id="BAA06606.1" FT /translation="ITLFEELHGR" FT unsure 39..40 FT /replace="caggcctg" FT /citation=[3] FT unsure 43..44 FT /replace="gggcgcgccc" FT /citation=[3] FT unsure 56 FT /replace="g" FT /citation=[3] FT CDS complement(79..1245) FT /codon_start=1 FT /transl_table=11 FT /gene="acyB1" FT /gene_synonym="carE" FT /product="4'-mycarosyl isovaleryl-CoA transferase" FT /function="4'-O-acylation of macrolide antibiotics" FT /db_xref="GOA:P21542" FT /db_xref="InterPro:IPR002656" FT /db_xref="UniProtKB/Swiss-Prot:P21542" FT /citation=[3] FT /citation=[4] FT /protein_id="BAA06607.1" FT /translation="MPLPKHLPALGGMRFISALLVFTSHISTQPFFKNTEINSALQFPL FT NRLGPLTVSFFFMLSGFVLTWAGLPDKSKVNFWRRRTVRAYSLHLPVLLVTLLIVLALN FT EPNMGRSVWDGLLTNLLLIQAWFPDHHEYGSMNPVAWSLSCELFFYAMFPFLFAFFTKV FT RTDRLWRWAAAVSVAAVSIPLVALLLPASPPLPWDPDMPQLRWWFIYMFPPVRLLEFVL FT GMLMAQIVIRGRWRGPRPLACVALFSAVFAVTFAVPNHYDPGALTVPVIALLLASVAVG FT DVRGVRSWLGTRTMVLLGELTFAFYLVHYLIIQYGHRFAGGKQGYYRQWDTPAAVGLTL FT LAFTLALGLSAFLHFFVEKPVMRTLGRPRRSPDAGSTPRSEPAPSGTP" FT RBS complement(1251..1255) FT -10_signal complement(1281..1287) FT unsure 1304 FT /replace="" FT /citation=[3] FT -35_signal complement(1306..1311) FT -35_signal 1475..1480 FT -10_signal 1493..1498 FT RBS 1559..1562 FT CDS 1569..2732 FT /codon_start=1 FT /transl_table=11 FT /gene="acyB2" FT /product="regulatory protein" FT /function="activation of acyB1 expression" FT /db_xref="UniProtKB/TrEMBL:Q56076" FT /citation=[4] FT /protein_id="BAA32409.1" FT /translation="MHSIPCGSKPSASMWDTGVHDDFDTHISETCSELFSSLRRADQRK FT RGEQYVRGLLTAQGRKTARNLAAFVGEGAADQNLHHFVAGSTWDWRSVRAALARYADQT FT VRGDAWVIRPMVVYKAGGRSVGVGRRFVPDLGRVVSCQQSYGLWLASDAMSAPVNWHLT FT LGGGPGDRHDRQLSAYGEEEKLVDLVAELTRSNRVLARPVVMDARIATLPRLVRALSAA FT DQSFLLRVSGDLPLALAGSRGQLDRRAQVWPAQHLMEQLKRLRRPVEWQGSISFVAPCN FT VVLTDQLPQRTLLLFGVWRANRRRPADLWLTDLTSWNRGALLRLAMLTCRVDADFARVS FT LGVGIRDFEGRSFQGWHRHVTLASIAHALRLSCTDTARTPTAPALSR" XX SQ Sequence 2749 BP; 476 A; 899 C; 970 G; 404 T; 0 other; gatcacactc ttcgaggaac tccacgggcg ctgacgcacg cggcgagggc gcgccccgca 60 ccggtcgtcc cgcgtcggct acggagtgcc ggacggggcg ggttcggacc tgggtgtcga 120 gccggcgtcc ggggaccgcc gcggccgtcc cagggttcgc atgaccggct tctccacgaa 180 gaagtgcagg aacgccgaca gccccagcgc cagcgtgaag gcgagcaggg tcagcccgac 240 ggcggccggt gtgtcccact gccggtaata gccctgcttc ccgccggcga agcggtgccc 300 gtactggatg atcaggtagt gcacgaggta gaaggcgaag gtgagttccc ccagcagcac 360 catcgtcctg gtccccagcc aggagcggac gccgcgcaca tcaccgacgg ccaccgaggc 420 gagcagcagc gcgatcaccg ggacggtcaa cgcgccgggg tcgtagtggt tcggcaccgc 480 gaacgtcacc gcgaacaccg ctgagaacag cgcgacgcag gccaggggac gcgggcccct 540 ccagcgtccc cggatcacga tctgggccat gagcatcccg agcacgaact ccagcagccg 600 caccggcggg aacatgtaga tgaaccacca ccgcagctgc ggcatgtccg ggtcccacgg 660 caggggcggg ctggccggca gcagcagtgc gaccaggggg atggagacgg cggccacgga 720 caccgcggcg gcccaccgcc agagccggtc cgtacggacc ttggtgaaga aggcgaagag 780 gaacgggaac atggcgtaga agaacagctc gcaggagagc gaccacgcca ccgggttcat 840 gctgccgtac tcgtggtggt cggggaacca tgcctggatc agcagcaggt tcgtgagcag 900 tccgtcccac accgatcggc ccatgttggg ctcgttgagg gccagcacga tcagcagcgt 960 caccagcagc acgggcaggt gcagcgagta cgcgcggacc gtgcgccgcc gccagaagtt 1020 caccttggac ttgtcgggca gacccgccca ggtgaggacg aaaccgctga gcatgaagaa 1080 gaacgagacc gtcagcgggc ccagccggtt cagcgggaac tgcagcgcgg aattgatctc 1140 ggtgttcttg aagaacggct gtgtcgatat atgggaggtg aataccagta gagcggagat 1200 gaaacgcatc ccgccgagcg cgggaagatg tttcggcagg ggcatgggtg acctcgcggc 1260 ggacgggtgg gcgggagcag atgtctaagg caccgcgcgg ccgccgggca aggcgtatct 1320 gacaaagagt gtgagccgcc gcgcgacagt ggtgtgttcc caccgctgca cggccggcga 1380 cggtggtttc gtcaccgggc gacgcggatt taatcatccg gaaacacgga cgacggttct 1440 tcgcggcggt gtgacgcgag gtgtgcggga agaattgtgt ggcctcggca cctggattta 1500 cggaacgaat taactgaggc tggccagcag gtcttttcca gtggatcaag cggcttgtga 1560 gggtcatagt gcacagtatt ccgtgtggct caaaaccgtc ggcctccatg tgggacaccg 1620 gtgtccatga cgacttcgat acgcacatct ccgagacctg ctcggagctg ttcagttcgc 1680 tgcgccgggc cgaccagcgc aagaggggcg aacagtacgt ccgtggtctg ctcaccgctc 1740 agggacgcaa gaccgcccgc aacctcgccg cgttcgtcgg tgaaggcgcc gcggaccaga 1800 acctccacca cttcgtggcc ggatccacct gggactggcg ctccgtgcgt gccgcgctgg 1860 cccgctacgc cgaccagacg gtacgcgggg acgcgtgggt gatccgcccc atggtggtct 1920 acaaggcagg gggacggtcg gtcggcgtgg gccggcgctt cgtacccgac ctggggcggg 1980 tggtcagctg tcagcagagc tacgggctct ggctcgcctc cgacgccatg tcggcaccgg 2040 tgaactggca tctgacgctg ggcggcggac cgggcgaccg tcacgatcgg cagctgagcg 2100 cgtacggcga ggaggagaag ctcgtcgacc tggtggcgga actcacgcgc tcgaaccggg 2160 tgttggcgcg tccggtggtg atggacgcgc ggatcgccac gctgccccgg ctggttcggg 2220 cgctgtccgc ggctgatcaa tcgtttctct taagggtgag cggcgatctt ccgctcgccc 2280 tcgccggcag ccggggccaa ctggacaggc gcgcgcaggt ctggcccgcc cagcacctca 2340 tggaacagct caagcggctc aggcgccctg tggagtggca gggctccatc agcttcgtcg 2400 ccccgtgcaa cgtggtgctg accgatcagc tgccgcagcg cacgctcctg ctgttcgggg 2460 tgtggcgcgc caaccgcagg cgacccgcgg acctgtggct caccgacctc acgtcctgga 2520 accgcggcgc actgctgcgg ctggccatgc tgacctgccg cgtggacgcc gacttcgccc 2580 gcgtcagcct gggcgtcggc atccgcgact tcgagggccg ctccttccag ggctggcacc 2640 gtcacgtgac actggcctcg atagcccacg ccctacgcct ttcctgcacc gacaccgccc 2700 gcacccccac ggccccggcc ctgtcccgct gacggtgagg tcacggggc 2749 // |
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