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EBI DbfetchID D28503; SV 1; linear; genomic DNA; STD; PRO; 5979 BP. XX AC D28503; XX DT 16-MAR-1994 (Rel. 38, Created) DT 12-MAY-2009 (Rel. 100, Last updated, Version 11) XX DE Clostridium josui hemA, hemC, hemD and hemB genes, partial and complete DE cds. XX KW . XX OS Clostridium josui OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-5979 RA Fujino E.; RT ; RL Submitted (08-FEB-1994) to the EMBL/GenBank/DDBJ databases. RL Contact:Emi Fujino Faculty of Bioresources, Mie University; 1515 Kamihama, RL Tsu, Mie 514, Japan XX RN [2] RP 1-5979 RX PUBMED; 7665501. RA Fujino E., Fujino T., Karita S., Ohmiya K.; RT "Cloning and sequencing of some genes responsible for porphyrin RT biosynthesis from the anaerobic bacterium Clostridium josui"; RL J. Bacteriol. 177(17):5169-5175(1995). XX FH Key Location/Qualifiers FH FT source 1..5979 FT /organism="Clostridium josui" FT /strain="FERM P-9684" FT /mol_type="genomic DNA" FT /db_xref="taxon:1499" FT CDS 1379..2926 FT /codon_start=1 FT /transl_table=11 FT /gene="hemA" FT /product="NAC(P)H-dependent glutamyl-tRNA reductase" FT /EC_number="6.1.1.17" FT /note="porphyrin biosynthesis" FT /db_xref="GOA:Q59292" FT /db_xref="HSSP:1GPJ" FT /db_xref="InterPro:IPR006367" FT /db_xref="UniProtKB/Swiss-Prot:Q59292" FT /protein_id="BAA05860.1" FT /translation="MQLGRHNSGSIKKRLEMYILSIISASLDYKSAAIDIRERFSYTST FT RIREILRRIKAADGVSGAVLLCTCNRTELYISGDNIENMNPALLLCQLSGEEDHKSLMT FT LFSIRHDSEAIFHLMEVACGLQSMVLFEDRVITQVKNAAAISREEKTIDSTLETLFRLC FT ITAAKKAKTEIKVKAVPTSAAERAITELSKKYCFTDKRILVIGNGEIGRLCCKKLIELG FT AEITITLRKYKHGEIIIPVGCNTIPYDEREEVLPLSDVVISATTSPHFTITYDMIEKLE FT RKPEIFVDLALPRDIESSISNFTGVELYNLDRFYTDYSVLNQKEVSKIREIINHFILQF FT EKWKDYREEAAFTKIPDLHNDTLYGRFPLFIDLSGKKVLVVGGGEIATRRVKTLLRFGA FT DIYLVAPHLTSELQEMLNCKLINYREGYYESQDIQNMFLVIAATNDRETNHKVYLDAKE FT KGIQMSIADCREECSFYFPAIFEFDGIVGGLVSQNGDNHSLVKSVAEQIRKIGQATD" FT CDS 2953..3840 FT /codon_start=1 FT /transl_table=11 FT /gene="hemC" FT /product="porphobilinogen deaminase" FT /EC_number="2.5.1.61" FT /note="porphyrin biosynthesis" FT /db_xref="GOA:Q59293" FT /db_xref="HSSP:1PDA" FT /db_xref="InterPro:IPR000860" FT /db_xref="UniProtKB/Swiss-Prot:Q59293" FT /protein_id="BAA05861.1" FT /translation="MVFDMKKIRIGSRDSKLAIIQSELIMSAIRKYDPDIELELITMKT FT TGDKILDKTLDKIEGKGLFVKELDNALYNNEVDITVHSYKDMPLEENPELPVVALSKRE FT DPRDAFILPQNGENGEPIGSSSLRRQLQLKELFPGCKTAPIRGNVQTRLKKLDSGEFSA FT IVLAAAGIKRLGLESRIGRYFSVDEILPAASQGIIAVQGRVGENFDFLKLFHSEESLCI FT SLAERTFVREMNGGCSTPIAAYATIQGSEIILKGLYCNETTGELRKECVSGNRNNPVEL FT GYELVKKMKSSKSI" FT CDS 3857..5371 FT /codon_start=1 FT /transl_table=11 FT /gene="hemD" FT /product="uroporphyrinogen-III synthase" FT /EC_number="4.2.1.75" FT /note="porphyrin biosynthesis" FT /db_xref="GOA:Q59294" FT /db_xref="HSSP:1CBF" FT /db_xref="InterPro:IPR003043" FT /db_xref="UniProtKB/Swiss-Prot:Q59294" FT /protein_id="BAA05862.1" FT /translation="MEHGFVALVGAGPGDKGLITIRGAELLSQADVVVYDRLVSQEIIK FT MIPTTAEKIDVGKENKFHPVKQEEINHILLEKSLEGKKVIRLKGGDPFVFGRGGEELEL FT LYENNIPFEVVPGVTSAVAALCYGGIPATHRDFCSSLHIITGHAREGGQLSIPFHELKE FT LNGTIVFLMGDSSLSYLMNGLINAGMEKDMPAAIVENGTRPNQRKLVATVGTLEQKALE FT MEIKSPAIIAVGKVCSLSEKFSWFMKKPLFGTKILVTRPKESSGTLVEKLRQLGAEPVE FT YPCIEVVPIPQNEKLYHACENIREYGWILLTSKNGIQIFFDYLNSKGLDARVLANTKIG FT TVGSQTAKALKEVGLISDFTPEIFDGRHLALGIAERVGENEKVLICDAAIASDDIVNIL FT RSNNIKFDRVPLYNTNYINENSNKVKKSIVHGELKYITFTSASTVEGFIASMKDIPLES FT LTAVCIGNKTAEAAKKYNLRYVVAEKSTIDSMIDKLLEIGGGNIYD" FT CDS 5364..>5979 FT /codon_start=1 FT /transl_table=11 FT /gene="hemB" FT /product="delta-aminolevulinic acid dehydratase" FT /EC_number="4.2.1.24" FT /note="porphyrin biosynthesis" FT /db_xref="GOA:Q59295" FT /db_xref="HSSP:1L6S" FT /db_xref="InterPro:IPR001731" FT /db_xref="UniProtKB/Swiss-Prot:Q59295" FT /protein_id="BAA05863.1" FT /translation="MIKRPRRLRTNEVLRKAVRETRLSTDSLIWPLFIVEGKNIKKEIS FT SLPGQYHFSPDMVGKAIEAALKADVKSVLLFGLPKHKDEKGSEAYNENGVLQQGIREIK FT QRYPQMQVITDICMCEYTSHGHCGILEGERVDNDRTLPYLEKIALSHVMAGADMIAPSD FT MMDGRIYALRSTLDKNGFTDIPIMSYAVKYASSFYGPFREAA" XX SQ Sequence 5979 BP; 2008 A; 961 C; 1394 G; 1616 T; 0 other; gatcaggctt ccattgaagc atggtactct gaattaaacc gtaacgataa gggaatagaa 60 gatactacta tatggcaaaa tatagaaata ccttcctatt gggaagacga gggcttgggc 120 aactttaacg gtgttgtctg gttcagaaag gaaattcata ttccccacac gcttgcaggt 180 ttacctgcaa gacttgtact aggaaacatt gttgatgaag acactaccta tatcaatggt 240 gtagaggtag gaacaacgcc aaatcagtat atctctagaa aatatagtgt tcaggagggc 300 ttactcaagg aaggtaaaaa tacaatcctg ctcagagtaa taaatatctc aggtaaagga 360 ggattttaca aaggaaagcc ctatcaactg gaagttggag atagtattat tgatttatct 420 ggtgaatggc agtatgtgat tggagcaaaa agcggcccta tgcctgggcc ggcctttgtt 480 cagtggcgtc ccttaggatt gtacaacgga atgattgctc ctgttacaag ttatgcgata 540 aaaggcttta tatggtatca aggggaagct aataccaaga atcctgtagg gtatgaaaat 600 ctcctaaagg cattgatttc agactggaga cagaaatggg gaatgggaaa tctgccgttt 660 ttgtatgttc aattgccgaa tttcatggag gcctcagaaa tacctgtgga aagcaagtgg 720 gcagagttga gggaagccca gcgaaagaca ctttccgtac cgaatacagg aatgattgtt 780 acaattgacc ttggagaatg gaatgatatt catccatcaa ataaaaagga tgtagggttt 840 cgcttggcac ttgcagccat gaaaaccgta tatggagaca aaaccattac tgcatacgga 900 ccaatgtata tattatcctt tactgatact ggaagtgggt tgattgttaa cagtggagaa 960 cggccgggag cttttgctat atcagggcca gatagaatat ttgttcgtgc tgatacagaa 1020 cttataggaa atgatgtggc agtctggagt gaaaagatag ctcacccggc ttacgttagg 1080 tatgcatggg ccgataatcc acagatgcaa acctttataa ccgtgagggg ttacctgctt 1140 cgccttttac aacggaatag cctttattga ctgttatata tcgaaattat aaataaaacc 1200 tttattgcta aaactttgaa ggttttattt ttttatcaac tattattaac tttgaaggtt 1260 cattataatg taacttgaaa agtaatgagc agtttacagt aaatttatgg gcagtagatg 1320 taggctgcgg tataatgaat attgatatga ttaaaaagga aagtttgtag tttcctaaat 1380 gcagctggga cggcataaca gcggcagtat caaaaaaaga ttggagatgt atattttgag 1440 tattatatca gcaagtttag actataaatc agctgccatt gatatcaggg aaagattcag 1500 ctatacatcc acaagaatca gagaaatact tagaaggata aaagcggctg acggtgtttc 1560 aggagctgta ctcctgtgta cctgcaacag aaccgaactt tatatttcgg gagataatat 1620 tgaaaatatg aatcctgccc tgcttttgtg ccagttgtca ggtgaggaag accataaatc 1680 cttaatgact ttatttagta taagacatga ttcagaggca atattccatc tgatggaagt 1740 agcttgcggg cttcagtcta tggtactttt tgaggatcga gtaataacac aggtaaaaaa 1800 tgcggctgct atttcccgag aggagaaaac cattgattca acattggaga cactgtttag 1860 actgtgtatt acagctgcca agaaagccaa aaccgaaatt aaggtaaagg cagtccctac 1920 ttcggcagca gaaagagcaa taacggaatt atcaaaaaag tattgtttta ctgataaaag 1980 aattcttgta atcggtaatg gcgaaatagg acggctgtgt tgcaaaaaac ttattgaact 2040 gggagcagaa ataacaatca cactcagaaa gtataaacat ggagaaataa ttattcctgt 2100 aggctgcaat acaattccct atgacgaaag agaagaggtt cttcctcttt cagatgtggt 2160 tataagtgca actacaagtc ctcacttcac tataacgtat gatatgattg aaaaacttga 2220 aagaaagcct gagatatttg ttgatttggc actgcccaga gatattgagt cgagcatttc 2280 aaattttacg ggagtagaat tatataattt ggacaggttc tacactgact attcagtact 2340 taatcaaaag gaagtaagta aaataagaga gataattaat cactttattt tacagtttga 2400 aaaatggaaa gattatcgtg aagaagcagc atttacaaaa attcccgatt tacataatga 2460 tactctatat ggaagatttc ctttgtttat tgacttatca gggaaaaaag ttctcgttgt 2520 gggaggcgga gagattgcaa cccgaagagt aaaaacacta ctgagattcg gggcagatat 2580 ttatctggta gctccacatc ttacatcgga gcttcaagaa atgttgaatt gtaagctgat 2640 taattacaga gaaggatatt atgaatcaca ggatattcaa aatatgtttc tggtgattgc 2700 tgctacaaat gatagagaga caaaccataa ggtatatctg gacgccaagg aaaagggcat 2760 acaaatgagt atagcggatt gcagagaaga gtgtagcttt tactttcctg caatattcga 2820 atttgatggt atagtcggtg ggctggtttc ccaaaatgga gataatcaca gtcttgtaaa 2880 atctgttgct gaacagattc ggaaaattgg acaggctaca gattgagaat tttatcattt 2940 gaaggaaagg ctatggtatt tgacatgaaa aaaatcagaa taggcagcag ggacagcaag 3000 cttgcaatta tacaatcaga gttaataatg tctgcaatca gaaaatatga ccctgacata 3060 gagctggaat tgattacaat gaaaactaca ggagataaga ttttggacaa aactcttgac 3120 aaaatagaag gaaagggact tttcgtcaag gaactggata atgcattata caataatgag 3180 gtggatataa ccgtacatag ttataaggat atgcctttgg aagaaaatcc ggagctgcct 3240 gttgtagccc tgtcaaagcg tgaagaccct agagatgcct ttattttgcc tcaaaatggt 3300 gaaaatggtg aaccaatagg aagttcaagt ctgagaagac agctccagct gaaagaatta 3360 tttccgggtt gtaagactgc tcctatccgg ggaaatgtac aaacccgact taaaaaactt 3420 gacagcggtg agttttcggc catagtactt gctgctgctg gaattaaaag gcttggtctt 3480 gaaagtcgta tcggcagata tttttcagtt gatgaaattc ttcctgcggc aagtcagggc 3540 attatagcgg tacagggcag agtcggagag aactttgatt ttctaaagct gtttcatagt 3600 gaggaatccc tttgtatttc actggcagaa agaactttcg tacgagaaat gaatgggggc 3660 tgcagcacac caatagcggc ttatgcaacc attcagggaa gtgaaataat tctaaagggc 3720 ttatactgca atgaaaccac aggagagtta cgaaaggaat gtgtttcggg aaatagaaat 3780 aatcctgtgg aattaggcta tgaactggta aaaaagatga aaagcagcaa gagtatttaa 3840 gcatggagga aaggttatgg agcatggatt tgtggcctta gttggtgcgg gaccgggaga 3900 taaggggctt attacaataa ggggagcaga actgctctca caggctgatg tggtagttta 3960 tgacagactg gtatcccaag aaattattaa aatgattcca actacggcag aaaagataga 4020 tgtgggaaaa gagaataaat tccaccctgt taaacaggaa gaaattaacc atatactcct 4080 agaaaagtca ttagaaggaa agaaggttat aaggcttaaa ggaggagatc ctttcgtatt 4140 tggcagggga ggagaagaac ttgagctgct gtatgaaaac aatattccct ttgaggtcgt 4200 tcccggagta acttcggcag tggcagcatt atgctacggg ggaataccgg ctacccacag 4260 ggatttttgc tcttcgctgc atattattac cggacatgct agagagggag gacagctttc 4320 aatcccattt catgagttaa aggaactaaa tggaaccatt gtttttctta tgggagattc 4380 ttcactatca tatttgatga atgggcttat aaatgcgggg atggaaaagg acatgcctgc 4440 tgccatagta gagaacggaa caaggccaaa ccagagaaaa ctggtagcca cagtgggtac 4500 actggagcaa aaggctttgg agatggaaat aaaatctcct gccattattg ctgtaggtaa 4560 ggtctgttct ctatctgaaa agttcagttg gtttatgaaa aagcctcttt tcggtacaaa 4620 aatactagtt acaagaccca aagaatcttc tggcacactt gtagaaaagc ttcgccaact 4680 gggtgcggag cctgtagagt atccctgtat agaggtagta cctataccac aaaatgaaaa 4740 gctctaccat gcatgtgaaa atatcaggga atatggctgg attttgctta ccagtaaaaa 4800 cggtatacag atattttttg attacttaaa ttccaaagga ttagatgcaa gagttcttgc 4860 aaatacaaaa atcggcacgg taggaagtca gacagcgaaa gctttaaaag aagtgggact 4920 gatttctgat ttcacccctg aaatctttga tggcaggcat cttgcactgg gaattgcaga 4980 gcgtgttggg gaaaatgaaa aggttctaat ttgtgatgct gcaatagcaa gtgacgatat 5040 tgttaacatt ttgagaagta ataatattaa atttgataga gttccattgt acaatacgaa 5100 ttatataaat gaaaacagta ataaggtcaa aaaatcgatt gtccatggtg aactcaagta 5160 cataaccttt acaagtgcat ccacagttga aggatttata gcatctatga aagacattcc 5220 tttggaaagt ctgacggctg tctgcattgg aaacaagaca gcggaagctg ctaaaaagta 5280 taacctgaga tatgtagtag ctgaaaagtc aacaattgat tccatgatag ataagctatt 5340 agaaatagga ggcggcaata tttatgatta agagaccaag aagattgaga accaatgaag 5400 tgctgcgaaa agcagtcagg gaaaccagac tatcaacgga ttcacttatt tggcctcttt 5460 ttatagtaga gggtaaaaat ataaaaaagg aaatcagctc ccttcccgga cagtatcatt 5520 ttagtccgga tatggttggg aaagcaattg aagctgcatt aaaagcagat gtaaaatccg 5580 tattgctatt tggacttcct aagcataagg atgaaaaagg ctctgaggca tacaatgaaa 5640 acggagtatt gcagcaggga atacgtgaaa taaaacagag gtatccgcag atgcaggtaa 5700 taacagatat ttgcatgtgc gaatacacat ctcacggcca ttgcggaatt ttggagggag 5760 aaagggttga taatgacaga acccttccct atctggaaaa aatagctttg tcccatgtaa 5820 tggcaggagc ggacatgatt gctccgtcgg atatgatgga tggtaggata tatgctctta 5880 ggtcaactct ggacaaaaac gggtttacag acattcctat tatgtcatat gccgtaaaat 5940 atgcatcgtc attttatgga ccattccgtg aggctgcag 5979 // |
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