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EBI DbfetchID D28467; SV 1; linear; genomic DNA; STD; PRO; 6936 BP. XX AC D28467; XX DT 16-MAR-1994 (Rel. 38, Created) DT 14-AUG-2007 (Rel. 92, Last updated, Version 7) XX DE Bacillus sp. XAL601 gene for alpha-amylase-pullulanase, complete cds. XX KW . XX OS Bacillus sp. XAL601 OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-6936 RA Imanaka T.; RT ; RL Submitted (08-FEB-1994) to the EMBL/GenBank/DDBJ databases. RL Tadayuki Imanaka, Faculty of Engineering, Hiroshima University, Department RL of Fermentation Technology; 1-4-1 Kagamiyama, Higashi-Hiroshima, Hiroshima RL 724, Japan (Tel:81-6-877-5111(ex.4353), Fax:81-6-876-2250) XX RN [2] RX PUBMED; 7986049. RA Lee S., Morikawa M., Takagi M., Imanaka T.; RT "Cloning of the aapT gene and characterization of its product, RT alpha-amylase-pullulanase (AapT), from thermophilic and alkaliphilic RT Bacillus sp. strain XAL601"; RL Appl. Environ. Microbiol. 60(10):3764-3773(1994). XX FH Key Location/Qualifiers FH FT source 1..6936 FT /organism="Bacillus sp. XAL601" FT /strain="XAL601" FT /mol_type="genomic DNA" FT /db_xref="taxon:460016" FT -35_signal 316..321 FT -10_signal 351..356 FT RBS 739..745 FT CDS 751..6849 FT /codon_start=1 FT /transl_table=11 FT /product="alpha-amylase-pullulanase" FT /note="AapT" FT /note="ORF" FT /db_xref="GOA:Q45643" FT /db_xref="HSSP:1JI2" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q45643" FT /protein_id="BAA05832.1" FT /translation="MVKGRWRKYVAYFSIFLLFAQLFSFSAIVRANEPMQSPVVNGNDV FT TFRYVGTGEEQSVLLAGSFNDWQTIELTKESDYIWSVTKTLPDGTYMYKFVVDGNWVAD FT PLNANQADDGHGGKNSVVVVGQPAQQQERVVTLVGNLQKELGHEKEWDPTATATVMKHE FT GNGLYTFTGTLPAGTYEYKIAINGSWDENYGAGGRNGGNISFTLEKETEVTFYYHDSTH FT AIADSTWYTPIPKEKQPRLVGTILPAIGYEKEVDGWTPETSTAFFTDDDFDSVYTFSAR FT VPKGTYEYKVVLGNSWDENYPQDNNAKLNVLEETTITFFFNAKTKELYTDYSPTGSDGN FT VDKDRLKHNTWDSLYRQPFGAVKAGTEVTLRLAAKKGDVTKANVYVKNTTTGTAKLYEM FT SKVGVIGDDEYWEATFTPEEKGLYGYKFIAMDGAAKAEYGEDTQEGQWGRAVDKNAELF FT QLTVYDPNYKTPDWMKQAVVYQIFPDRFFNGNPNNDKVKEYGRGNEPIEYREWSQLPDN FT PRMKDTPGYDGDGFWNNDFFGGDIAGIEQKLDYLQSLGVNTIYLNPIAHAPSSHKYDAQ FT DFKEIDPMFGTPEEFESFVQAIASRGMHLILDGVFNHVSDDSIYFDRYGKYPTVGAYEY FT WSAVYDLMNEKGLSEEQARAQVEQKFKDEGQQFSPYGFHLWFNIENEKVNGVYKYQSWW FT GFDSLPEFKSVTGTKVPYPSELNNEQLANYIFYERDSVAKSWITRGASGWRLDVANEVD FT TEFWREFRKELLQSDYDRGPTLKNGQQPLILGEIWDDASKYFLGDQYDSVMNYRFRGAV FT LDFLRNGKAEEIDARLTAVREDYPEEAFYALMNLIGSHDTARAIFLLGNGTDSYERAEL FT DPNYNEQLGKQRSKVAVIFQMGYPGAPTIYYGDEAGVTGSKDPDNRRTYPWGSEDKELI FT AHYQKVGKIRTKHQDVLAKGTIETVYAKGDVYVFARQYGKDVALIAMNRGDSAQTVQLD FT VSSIIPNGTSFVDELHGEYEVTVSDGKVQLNIPALDGRMLFGKVTVNMPNEVTNVQATA FT GVGQVTLAWEGDASTYRIYQSTLKGAGYELVKETNEKTAVIDNLKNGTAYYFAITAVDE FT NGNESAKVETNRVVPHYPLAEEHVALLSEVNGGVLDLAALITVEAKVQIDNVTKHGLAD FT GLQAVLQVKKPNSDKWEDIQATYDRQDGDANVFRASFTPLQAGTYTYRYGFTTNLGDSW FT VYTEEKTFTLTANEADQQAPAKGIHLLQPDVESGQVNLSWSFIERDDHDAYMVVIERDG FT QVIHTTTTISTSFTDYDVENGKTYTYVVKLYDRAGNVVASKDVQITPDIVMVQVTFKVK FT APSYTPLDTRITIPNSINGWNTGAWEMTRGGAVTPDWEFTTELQEGETITYKYVKGGSW FT DQEGLADHTRDDQTDDDVSYYGYGAIGTELKVTVHNQGNNKMVIQDYILRWIDMPVVVE FT DVTKDGDRVTIKGNAIKDGVLTINKERVTIQDDMSFTYTFTPAANQKEIAIHIEPSERS FT KSDIFKNDGGAIAKNTKDYVLNLDTKQLREGVLQEDTGSGTTPGSGTTPGSGTTPGSGT FT TPGSGTTPGSGTTPGSGTTPGSGTTPGSGTTPGSGTTPGSGTTPGSGTTPEKGAVVLQP FT QVETKEKDGKIVEKVATISSNEVTSIVNSLSNENKQVVVSLGSLAKGVTTKVDVPATLF FT TQAANKQGEATIVSATEQATYKLPAKEVQASLAAIAKTLGATVEQVNISIEMRVKDAPQ FT LRAKALSDAVEFHVVAKANGKEQVIDRFTQYVEREIVLKQAVNASRSIVVRVNDDGSIT FT PVPTTFVGNKAVIKSLTNSTYVVVEGTHTFSDIEKHWAKGYIETLAAKQLVKGMTETAY FT RPNEQMTRAQFAVLLVRALALPHETYDGRFADVKGTEWFNKNGELAAAVKLGIIQGKTA FT NTFAPNEPITRVQAAVMIERALKLSFVGYDEATNDKTKKATDFRDAKQLPTWAKQAIEA FT VYQAGIMQGRDNGSFDPTGHMTRAEMAKVLAEFLGKVKLM" FT terminator 6852..6884 XX SQ Sequence 6936 BP; 2252 A; 1302 C; 1682 G; 1700 T; 0 other; caaatcaatt gatttagggt tatcgaaaac cttccttcca aatatcttct ttaattgact 60 agaaccgttg ggttttaagt caaaccaggc attacttaac aactttttgc tagtcggtgg 120 aacaaagtgc tcaatcgtgc cattatcatt ttgtcgtaac aagtcaatat cttccccatt 180 attttctaca tattcaagat accatttatc aatatcctct tgagttggat tagtaatgcc 240 tttttgtcta agttcatcta acatcatttg atagttttca atagcctgga gactacgttc 300 tttcccccat ctccattgac cttctgtcgg agtaacccca aataattcat atctcatagt 360 tttacggtct gtacctctcc aatacgggat ttgtactttg gattgtgccg tcgaatcaaa 420 tataccgtca aaccttgtct gctttacgaa atcgtgagca tccataccgg caagtattgg 480 atttatcaag tggcggtcgg acggaaatcc tcgaaaaaat ggatcggttt acaccccaag 540 acgtacaaga gaaattactt gtgatggtta aaagtcctga tgggtaggga cggtgctgtc 600 aacatcacaa gcccttccaa catgggggaa gaggaagaac actccgagaa aggaggggga 660 gtaagtgagg ctgtttgcat cccccttact gttatacccc tttgtgcaaa cggtttcact 720 tgaaagaaaa tttaaataaa ggaggagcaa atggtgaagg gaagatggag aaagtatgtc 780 gcttatttct caatcttttt gctttttgca cagttgtttt catttagcgc aatcgttcgc 840 gcaaatgaac caatgcaaag ccctgttgtc aacggaaacg atgtgacgtt tcgctacgtt 900 ggaacgggag aagagcagtc tgttttgcta gccggatcgt ttaatgactg gcaaacaatt 960 gaattgacga aagaaagcga ttatatttgg tcagtaacaa aaacgcttcc agatggaacg 1020 tacatgtaca aatttgttgt agatggaaac tgggtggctg atccgttaaa tgccaatcaa 1080 gctgatgacg ggcatggcgg aaaaaatagc gttgtcgtag ttggtcaacc tgctcagcag 1140 caggagcgtg ttgtgacgct cgttggcaat ttacaaaagg agcttgggca tgaaaaagag 1200 tgggatccga cagcgacagc taccgtcatg aagcatgaag gcaatggatt gtacacattc 1260 acaggaacgc ttccagcagg tacgtatgaa tacaaaatcg ccataaacgg aagctgggat 1320 gaaaactatg gtgcaggtgg acggaacggt ggaaacattt ctttcacgct tgaaaaagag 1380 acagaagtaa cgttctatta ccacgatagt actcatgcga tcgctgattc aacgtggtat 1440 acgccgattc caaaagaaaa acagccgcga ttagtcggaa cgattttgcc tgcgatcggc 1500 tatgaaaaag aagtcgatgg ttggacgcca gagacgtcaa ccgcattttt tacagacgat 1560 gactttgact ccgtttatac atttagcgca cgtgtgccaa aaggaacata tgaatataaa 1620 gtagtgttag ggaatagttg ggatgaaaat tatcctcaag ataataatgc taaattaaac 1680 gtgttagaag aaacgacaat tacgttcttc tttaacgcaa aaacaaagga gttatataca 1740 gactacagtc caacaggttc ggatggcaac gttgacaaag atcgtttaaa gcataatacg 1800 tgggactctt tatatcgtca gccgtttgga gcagtgaaag caggaacgga agtgacgtta 1860 cgtttagctg cgaaaaaagg tgatgtaacg aaagcgaacg tgtatgtgaa gaatacaacg 1920 acaggaacgg cgaagttgta cgagatgagc aaagtaggag tgatcggaga cgatgaatat 1980 tgggaagcga cattcactcc tgaagaaaaa gggttatatg gctataaatt tatcgcgatg 2040 gacggtgcag ctaaagcaga gtatggtgag gatacacaag aaggtcaatg gggacgagcg 2100 gtagataaaa acgcagaatt attccaattg actgtatacg acccaaacta taaaacgcca 2160 gattggatga aacaggcggt tgtatatcaa attttccctg atcgtttctt taatgggaat 2220 ccaaataacg acaaagtgaa agaatatggg cgtggaaatg agccaattga gtatcgggag 2280 tggtcgcaac ttccagataa tccacgtatg aaagatactc ctggatatga tggcgacggg 2340 ttttggaata acgacttttt tggtggagac attgcaggaa ttgaacaaaa gttagattac 2400 ttgcagtctt taggtgtaaa tacaatttac ttaaacccga ttgcgcatgc gccatcaagc 2460 cataagtatg atgcacaaga ttttaaagaa attgatccga tgttcggaac acctgaagag 2520 tttgaatcat ttgtgcaagc gattgcatct cgtggtatgc acttaatttt agacggtgta 2580 tttaatcacg tatcagacga ttccatttat tttgaccgct atggaaaata tccaaccgtc 2640 ggagcatatg aatattggtc ggctgtgtat gatttgatga atgaaaaagg attatcagaa 2700 gaacaagctc gtgctcaagt agaacaaaag tttaaagatg agggacaaca gttcagtcca 2760 tacggatttc atttatggtt caatatcgag aatgaaaaag taaacggtgt gtacaaatat 2820 caatcgtggt ggggatttga tagtttacca gaatttaagt ctgtcactgg aacgaaagta 2880 ccgtatccga gcgaattaaa taatgagcaa ctcgcaaact acatttttta cgagcgagat 2940 tccgttgcta aaagttggat tacgcgcgga gcatccggtt ggcgtttaga tgtagccaat 3000 gaggtggata cggagttttg gcgtgagttt cgtaaagagt tgttgcaaag tgactatgac 3060 cgtgggccaa cgctcaaaaa cggacaacag ccgttaattc tcggtgaaat ttgggatgat 3120 gcatcaaaat acttccttgg tgatcaatac gattcagtca tgaactaccg tttccgcgga 3180 gctgtattag actttttacg aaacggtaaa gcagaggaaa tcgatgcgcg attaacagct 3240 gttcgtgaag attatccgga agaagctttt tatgcgctaa tgaaccttat cggctcacat 3300 gatacggcgc gtgccatctt cttgcttggg aacggaacgg attcatacga acgtgctgaa 3360 cttgatccga actataatga gcaacttggt aaacaacgtt ccaaagtggc tgtaattttc 3420 caaatgggtt atccgggagc accaacgatt tactacggcg atgaagcagg tgtaacaggc 3480 tctaaagatc cagacaatcg tcgtacgtat ccgtggggaa gcgaagacaa ggagcttatt 3540 gcacattatc aaaaagtagg taagattcgc acgaagcatc aagatgtatt agcgaaagga 3600 acaattgaga cagtatatgc gaaaggtgat gtgtacgtct ttgcccgtca gtacggaaaa 3660 gatgttgcgc ttatcgcgat gaaccgtggg gatagcgcgc aaacggtaca gttggatgtt 3720 tcttcaatca ttccgaatgg aacaagcttt gttgatgaac tacatggcga atatgaagtg 3780 acagtatcgg acggaaaggt acagctaaac attcctgccc tcgatggacg catgttgttt 3840 ggaaaagtaa ctgttaatat gccgaatgag gtgacgaacg ttcaagcgac agcaggcgtt 3900 ggacaagtga cgttagcgtg ggaaggcgat gcatcaacgt accgtattta tcaatctaca 3960 ttaaaaggtg caggttatga gcttgtgaaa gaaacgaatg aaaagacagc tgtgatagac 4020 aatttgaaaa acggtaccgc atattacttt gcgattacag ctgttgatga aaacggaaat 4080 gagtcggcta aagtagaaac gaatcgcgtc gtgccacatt atccattagc agaagaacac 4140 gttgctttac tttctgaagt gaacggtgga gtgctcgatt tagctgcttt aattacggtt 4200 gaagcgaaag tgcaaatcga taatgtaacg aaacatgggt tagctgacgg tttacaagcg 4260 gtgttgcaag tgaaaaaacc gaatagcgac aaatgggaag acatacaagc aacgtacgac 4320 cgacaagatg gcgatgcgaa tgtattccgt gcatcgttca ctccgctaca agcaggaacg 4380 tacacgtatc gttatggctt tacaacaaat ctcggtgaca gttgggtgta tacagaagaa 4440 aaaacgttta cactcactgc aaatgaggca gatcaacaag caccagcaaa gggcattcat 4500 ctcttgcaac cagatgtcga atctggtcaa gtaaatttat catggtcgtt tattgaaaga 4560 gacgatcatg atgcttatat ggtagtcatt gagcgcgacg gtcaagtgat tcatacgaca 4620 acaacgatta gcacatcatt tactgattat gatgtcgaaa acggaaaaac atatacgtat 4680 gttgtgaagt tgtatgaccg tgctgggaac gttgtcgcat caaaggacgt gcaaattacg 4740 ccagacatcg tgatggttca agtgacgttt aaagtgaaag cgccaagcta tacaccgtta 4800 gatacgcgca ttacgattcc aaatagtatc aacggttgga atacaggtgc atgggaaatg 4860 acgcgcggtg gagcggtcac acctgattgg gaatttacaa cggagcttca agaaggcgaa 4920 acaattactt ataagtatgt aaagggtggt tcatgggatc aagaagggct agctgatcat 4980 acgcgtgacg atcaaacaga tgatgacgtc agctactacg gttacggtgc gattggtaca 5040 gaattaaaag tgactgtgca caatcaaggt aataataaaa tggtcatcca agactatatt 5100 ttacgttgga ttgacatgcc tgtcgttgtt gaagatgtca ccaaagacgg tgatcgtgtg 5160 acgattaaag gaaatgcgat taaagatggt gtattaacaa ttaataaaga gcgtgtaaca 5220 attcaagacg acatgtcatt tacgtacacg ttcacaccag cagcaaacca aaaagaaata 5280 gcgatccata ttgaaccgtc ggaaagaagc aaatctgata tattcaaaaa tgacggtggt 5340 gcgattgcga aaaatacgaa agattacgta ttaaacctcg acacaaaaca attgcgtgaa 5400 ggggtattac aagaagatac aggcagtggc acaacaccag gtagcggcac aacaccaggc 5460 agtggcacaa caccaggtag tggcacaaca ccgggtagcg gcacaacacc aggtagtggc 5520 acaacaccag gtagtggcac aacaccaggt agcggtacaa caccaggcag tggcacaaca 5580 ccgggtagcg gcacaacacc aggtagtggc acaacaccag gtagtggcac aacaccagaa 5640 aaaggtgcag ttgttttaca accacaagta gagacgaaag aaaaagacgg caaaatcgta 5700 gaaaaagtgg cgactatttc atcaaatgaa gtgacttcta ttgttaactc actttcaaat 5760 gaaaataaac aagttgtcgt ttctctcggt tcgcttgcaa aaggtgtgac tacaaaagta 5820 gatgtaccag ctacattgtt tacacaggcg gcaaataagc aaggagaagc aacgattgtg 5880 agtgcaactg agcaggcgac atacaaattg ccagcaaaag aagtacaagc gtctctggca 5940 gcgattgcaa aaacactcgg tgcaacagtc gaacaagtaa acatctcgat cgaaatgaga 6000 gtaaaagatg ctccacaact acgtgcgaaa gcattatctg atgcagtaga gtttcatgtt 6060 gtggcgaagg caaacggaaa agaacaagtg attgatcgtt ttactcaata tgttgaacga 6120 gaaatcgtat taaaacaagc ggtgaatgca agtcgttcta ttgtggtgcg cgtaaacgat 6180 gacggttcca ttacacctgt accgaccaca tttgttggca acaaagcggt catcaagtca 6240 ttgacgaact cgacatacgt cgtcgttgaa ggaacacata catttagcga catcgagaaa 6300 cattgggcga aaggatatat cgaaacattg gcagctaaac agcttgtgaa aggtatgacg 6360 gaaacagcat accgaccaaa tgagcagatg acacgtgccc agtttgctgt gttgcttgta 6420 cgtgcattag cattgccaca tgaaacatat gacggtcgat ttgccgatgt aaaaggcaca 6480 gaatggttta acaaaaacgg tgaattagcg gctgcagtca aactaggtat cattcaagga 6540 aaaacagcca atacgtttgc gccaaatgag ccaatcactc gcgtgcaagc agctgttatg 6600 atcgagcgag cattgaaact ttcgtttgtt ggctatgatg aggcaacaaa cgacaaaacg 6660 aaaaaggcaa cagatttccg cgatgcaaaa caattgccaa catgggcaaa acaggcgatt 6720 gaagcagtat accaagcagg aattatgcaa ggacgggata atggaagttt tgatcctacg 6780 ggtcatatga cgcgcgccga aatggcgaaa gtattggcgg agtttttagg aaaagtaaaa 6840 ttgatgtaac gaaagggagg cctccgatat ggaagcctcc ctttacttca ccgtcgaaac 6900 ttcaacccca agcggcaagg cagctccgcc tgcatg 6936 // |
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