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EBI DbfetchID D28121; SV 1; linear; genomic DNA; STD; PRO; 4200 BP. XX AC D28121; XX DT 25-FEB-1994 (Rel. 38, Created) DT 26-FEB-2008 (Rel. 94, Last updated, Version 7) XX DE Geobacillus stearothermophilus genes for endo-1,4-beta-xylanase, xylan DE 1,4-beta-xylosidase, complete cds. XX KW . XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-4200 RA Nanmori T.; RT ; RL Submitted (26-JAN-1994) to the EMBL/GenBank/DDBJ databases. RL Contact:Takashi Nanmori Kobe University, Faculty of Agriculture; 1-1 RL Rokkodai-cho, Nada-ku, Kobe, Hyogo 657, Japan XX RN [2] RX PUBMED; 8074507. RA Baba T., Shinke R., Nanmori T.; RT "Identification and characterization of clustered genes for thermostable RT xylan-degrading enzymes, beta-xylosidase and xylanase, of Bacillus RT stearothermophilus 21"; RL Appl. Environ. Microbiol. 60(7):2252-2258(1994). XX FH Key Location/Qualifiers FH FT source 1..4200 FT /organism="Geobacillus stearothermophilus" FT /isolate="No.21" FT /mol_type="genomic DNA" FT /note="synonym: Bacillus stearothermophilus" FT /db_xref="taxon:1422" FT TATA_signal 765..770 FT RBS 821..826 FT /note="SD-sequence" FT CDS 835..2952 FT /codon_start=1 FT /transl_table=11 FT /product="xylan 1,4-beta-xylosidase" FT /EC_number="3.2.1.37" FT /note="b-xylosidase" FT /note="extracellular enzyme" FT /note="xylanase gene cluster" FT /db_xref="GOA:P45702" FT /db_xref="InterPro:IPR000852" FT /db_xref="UniProtKB/Swiss-Prot:P45702" FT /protein_id="BAA05667.1" FT /translation="MPTNLFFNAHHSPVGAFASFTLGFPGKSGGLDLELARPPRQNVLI FT GVESLHESGLYHVLPFLETAEEDESKRYDIENPDPNPQKPNILIPFAKEEIQREFHVAT FT DTWKAGDLTFTIYSPVKAVPNPETADEEELKLALVPAVIVEMTIDNTNGTRARRAFFGF FT EGTDPYTSMRRIDDTCPQLRGVGQGRILSIVSKDEGVRSALHFSMEDILTAQLEENWTF FT GLGKVGALIVDVPAGEKKTYQFAVCFYRGGYVTAGMDASYFYTRFFQNIEEVGLYALEQ FT AEVLKEQSFRSNKLIEKEWLSDDQTFMMAHAIRSYYGNTQLLEHEGKPIWVVNEGEYRM FT MNTFDLTVDQLFFELKLNPWTVKNVLDLYVERYSYEDRVRFPGEETEYPSGISFTHDMG FT VANTFSRPHYSSYELYGISGCFSHMTHEQLVNWVLCAAVYIEQTKDWAWRDKRLAILEQ FT CLESMVRRDHPDPEQRNGVMGLDSTRTMGGAEITTYDSLDVSLGQARNNLYLAGKCWAA FT YVALEKLFRDVGKEELAALAGEQAEKCAATIVSHVTDDGYIPAIMGEGNDSKIIPAIEG FT LVFPYFTNCHEALDENGRFGAYIQALRNHLQYVLREGICLFPDGGWKISSTSNNSWLSK FT IYLCQFIARHILGWEWDEQGKRADAAHVAWLTHPTLSIWSWSDQIIAGEITGSKYYPRG FT VTSILWLEEGE" FT TATA_signal 2196..2201 FT RBS 2939..2944 FT /note="SD-sequence" FT CDS 2955..3947 FT /codon_start=1 FT /transl_table=11 FT /product="endo-1,4-beta-xylanase" FT /EC_number="3.2.1.8" FT /note="b-xylosidase" FT /note="extracellular enzyme" FT /note="xylanase gene cluster" FT /db_xref="GOA:P45703" FT /db_xref="HSSP:1HIZ" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P45703" FT /protein_id="BAA05668.1" FT /translation="MCSSIPSLREVFANDFRIGAAVNPVTLEAQQSLLIRHVNSLTAEN FT HMKFEHLQPEEGRFTFDIAIKSSTSPFSSHGVRGHTLVWHNQTPSWVFQDSQGHFVGRD FT VLLERMKSHISTVVQRYKGKVYCWDVINEAVADEGSEWLRSSTWRQIIGDDFIQQAFLY FT AHEADPEALLFYNDYNECFPEKREKIYTLVKSLRDKGIPIHGIGMQAHWSLNRPTLDEI FT RAAIERYASLGVILHITELDISMFEFDDHRKDLAAPTNEMVERQAERYEQIFSLFKEYR FT DVIQNVTFWGIADDHTWLDHFPVQGRKNWPLLFDEQHNPKPAFWRVVNI" XX SQ Sequence 4200 BP; 1112 A; 907 C; 1057 G; 1124 T; 0 other; ggatcctttt ttatgcaata ctgatcgcct tagctctttt tcaagtgttg cctcttgtct 60 ggttgctctt tttctcattg aaaaataacc aggaagtatt caatacaccg ttgctttcgc 120 ctccatcacc accgcggtgg aaaattatgt aaaagtttgg gtggaaggca acattgggca 180 gtactttttc aacagtgtct ggattacgac cgtctctgtc attttgactg tattgcttgc 240 tagtttcgcg acctttgcca ttacacgcat gaattggaag tggaaacatg ttgtgcttgg 300 actttttatg gtcggtttaa tgattccgat tcattcgacg ttaatcccat tgttcagctt 360 ttttatgaaa gtgaagctca tcgatcatcc gttatcgatt gtcttaacca atattggatt 420 taacttaccg attacatcat gatcttgctc ggtttttacc agtcgcttcc gcgtgagttg 480 gaagaatcag cggtgatgga tgggtgttcc gttcatcgta tgtttttccg gatcattttg 540 ccgatgacgg caccggtgat cgttacgacg acgattatca acatgattta caactggaac 600 gagttcgttt ttgtgaacac atttattagc tcagatgagt ttaaaacgtt aactgtcggt 660 attcaaaact ttatcggcca gtatacgacc gattggggag cgattggtgc cactttaatg 720 atcagcgttc tgcctatttt gatcgtcttc ttcttcttaa gcgataaaat catggaaggt 780 attactgcag gagcgattaa aggataaatg tagaaaaagg ggagagaagt aaccatgcca 840 accaatctat ttttcaacgc ccaccactca ccggttggag cgtttgccag ctttacatta 900 ggatttccag gaaaaagcgg gggattggat cttgaactcg cccgtccccc gcggcaaaac 960 gtattgattg gtgtcgaatc gttacatgaa tcgggcttat atcatgtcct tccgtttttg 1020 gaaacagccg aagaagatga aagcaaacgg tatgacatcg aaaatcctga cccgaatccg 1080 caaaaaccga acatcttaat cccatttgcc aaagaggaga ttcaacgtga atttcatgtg 1140 gccacagata cgtggaaggc tggagattta acgtttacga tttattctcc tgtaaaagcg 1200 gtgccaaatc cggaaaccgc ggacgaggaa gaactcaagc tggcgttggt tccagctgtc 1260 atcgtggaga tgacgattga taatacaaat ggaacaaggg cccggcgggc gtttttcggg 1320 ttcgaaggca ccgatccgta tacttcaatg cggcggatcg atgacacatg cccgcaactg 1380 cgcggggtcg gtcaagggcg gattttgagc attgtatcca aggacgaggg tgttcgctca 1440 gcgctgcatt ttagcatgga ggatatctta acggcacagc tggaagaaaa ctggacgttt 1500 gggcttggca aagtgggcgc cttaattgtc gatgtgccgg caggcgaaaa gaaaacttat 1560 caatttgcgg tttgttttta ccgaggcggg tacgtgacgg cggggatgga tgcttcctat 1620 ttttataccc gtttctttca aaatattgag gaagtcggcc tttatgccct ggagcaggcg 1680 gaagtattga aggagcaatc gttccgttcc aataagctga ttgaaaaaga atggctgtct 1740 gatgatcaaa catttatgat ggcgcacgcc attcgcagct actacggcaa tacgcagctg 1800 ttagagcacg aggggaaacc gatttgggtg gtcaacgaag gcgagtaccg gatgatgaat 1860 acgtttgatt tgaccgttga ccaactcttt tttgaactga aactgaatcc atggacggtc 1920 aaaaatgttc tcgatttgta cgtcgagcgc tacagttacg aggatcgcgt tcgttttccg 1980 ggagaagaga cagaatatcc gagcggcatc agttttactc acgacatggg agtagccaat 2040 acgttctcgc gcccgcacta ctcgtcatac gagctgtatg gcattagcgg ctgcttctcg 2100 cacatgacgc acgaacagct tgtcaactgg gtgctttgcg cggcggtgta cattgaacaa 2160 acgaaagact gggcatggcg cgacaagcgg cttgctattt tggagcaatg cctagaaagc 2220 atggttcgcc gcgatcatcc cgatccggaa caacgaaatg gcgtaatggg actcgacagc 2280 acccgcacga tgggcggggc ggaaattacg acatatgaca gtttggacgt ttccctcggc 2340 caagcccgca acaatttata tttagcaggt aaatgttggg cggcctatgt agcgcttgaa 2400 aaattgttcc gtgatgtcgg caaggaagaa ttggccgcgc tggcgggaga gcaggcggaa 2460 aaatgcgccg cgacgatcgt cagccatgta accgatgacg ggtatattcc ggcgatcatg 2520 ggagaaggaa acgactcaaa aatcattccg gctattgagg ggcttgtgtt cccttatttc 2580 accaattgcc atgaagcgtt ggacgaaaac ggacgctttg gagcatatat tcaagcgttg 2640 cgcaaccatt tgcaatacgt gttgcgggaa ggaatttgcc tgttcccgga tggaggctgg 2700 aaaatttcct caacgagcaa caactcatgg ctaagtaaaa tttacctatg tcagttcatt 2760 gcccgtcata ttttaggctg ggaatgggat gaacaaggca aacgggccga tgccgcccat 2820 gtcgcttggc tcacccatcc gacattatcc atttggagct ggagtgatca aatcatcgcg 2880 ggtgaaatta ccggcagcaa atattacccg cgcggcgtga caagtatttt gtggcttgag 2940 gagggagaat aacgatgtgc tcatccatcc cgtcccttcg cgaagtgttt gccaatgatt 3000 ttcgcatcgg ggcagcagtc aatccagtga cgctagaagc ccaacaatcg ctgttgatcc 3060 gccatgtaaa cagccttacc gccgaaaacc atatgaagtt tgaacatctt cagccagagg 3120 aggggcggtt tacgtttgac atcgcgatca aatcatcgac ttcgccgttc tcatcacatg 3180 gcgttcgcgg acatacgctc gtgtggcata accaaacccc gagctgggtg tttcaagaca 3240 gccaagggca tttcgtcggc agagatgtgt tgctggaacg gatgaaatct catatctcca 3300 cggttgtaca gcgatacaaa gggaaagtct attgttggga tgtcatcaac gaagcggtcg 3360 ccgatgaggg gagcgaatgg ctgcgctcct caacgtggcg acaaatcatc ggcgatgatt 3420 ttattcagca ggcgtttctt tatgcccatg aagcggaccc agaggcgttg ctgttttaca 3480 acgactataa tgaatgtttt ccggaaaaac gcgagaaaat ttacacacta gtaaaatctt 3540 tgcgtgacaa aggaattccc attcacggca tcggcatgca ggcgcactgg agcctgaacc 3600 gcccgacgct tgatgaaatt cgcgcggcga ttgagcgata tgcgtctctc ggagtcattc 3660 tccatattac cgaacttgat atatcgatgt ttgaatttga cgatcatcga aaggacttgg 3720 ctgctcctac aaacgaaatg gtcgaacggc aggcagagcg gtacgaacaa attttctctc 3780 tattcaagga atatcgtgat gtcattcaaa atgtcacgtt ttggggaatt gccgatgacc 3840 acacgtggct tgaccacttc cctgtgcaag gaagaaaaaa ttggcccctt ttgttcgatg 3900 aacaacacaa ccccaaaccg gctttttggc gggtggtgaa tatctaaatt tctagttgct 3960 ttgaaatcat actatatagt gaagagggga aaaaatagag tttagttctc ccctcttcca 4020 taataataga aaactttcag aaaagtattg aatatgggga ggtttttcgt tataataaaa 4080 acaaaactag tatactagtg ttactagtaa tcaagtatcg cgtaagattg tacgtttgcc 4140 tttttgaact agaagatatt attttcaaac tagtatacta gaatgttaat tttgtgatcc 4200 // |
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