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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   D26392; SV 1; linear; mRNA; STD; PLN; 1499 BP.
XX
AC   D26392;
XX
DT   04-FEB-1994 (Rel. 38, Created)
DT   29-OCT-2007 (Rel. 93, Last updated, Version 6)
XX
DE   Cucumis sativus mRNA for monodehydroascorbate reductase, complete cds.
XX
KW   .
XX
OS   Cucumis sativus (cucumber)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids;
OC   eurosids I; Cucurbitales; Cucurbitaceae; Cucumis.
XX
RN   [1]
RP   1-1499
RA   Sano S.;
RT   ;
RL   Submitted (04-JAN-1994) to the EMBL/GenBank/DDBJ databases.
RL   Satoshi Sano, Kyoto University, The Research Institute for Food Science;
RL   Uji, Kyoto 611, Japan (Tel:81-774-31-8119, Fax:81-774-33-3004)
XX
RN   [2]
RA   Sano S., Asada K.;
RT   "cDNA cloning of monodehydroascorbate radical reductase from Cucumber: a
RT   high degree of homology in terms of amino acid sequence between this enzyme
RT   and bacterial flavoenzymes";
RL   Plant Cell Physiol. 53:425-437(1994).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1499
FT                   /organism="Cucumis sativus"
FT                   /mol_type="mRNA"
FT                   /dev_stage="seedling"
FT                   /clone_lib="lambda gt22A"
FT                   /clone="pCMR31"
FT                   /db_xref="taxon:3659"
FT   CDS             20..1324
FT                   /codon_start=1
FT                   /product="monodehydroascorbate reductase"
FT                   /EC_number="1.6.5.4"
FT                   /note="MDA reductase"
FT                   /note="NAD(P)H-binding, FAD-binding"
FT                   /note="flavin-containing oxidoreductase"
FT                   /db_xref="GOA:Q42711"
FT                   /db_xref="InterPro:IPR001327"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q42711"
FT                   /protein_id="BAA05408.1"
FT                   /translation="MADETFKYVILGGGVAAGYAAREFVKQGLNPGELAIISKEAVAPY
FT                   ERPALSKAYLFPESPARLPGFHVCVGSGGERLLPDWYKEKGIELILSTEIVEADLPAKR
FT                   LRSAHGKIYNYQTLIIATGSTVIKLSDFGVQGADAKNIFYLREIDDADQLVEAIKAKEN
FT                   GKVVVVGGGYIGLELGAALRINNFDVSMVYPEPWCMPRLFTPEIAAFYEGYYAQKGITI
FT                   IKGTVAVGFTVDTNGEVKEVKLKDGRVLEADIVVVGVGARPLTSLFKGQIVEEKGGIKT
FT                   DEFFKTSVPDVYAVGDVATFPLKLYNELRRVEHVDHSRKSAEQAVKAIKASEEGKAIEE
FT                   YDYLPYFYSRSFDLSWQFYGDNVGDAVLFGDNSPDSATHKFGSYWIKDGKVVGAFLESG
FT                   SPEENKAIAKVARIQPSVESSDLLLKEGISFASKV"
FT   polyA_signal    1471..1476
FT   polyA_site      1499
XX
SQ   Sequence 1499 BP; 436 A; 253 C; 372 G; 438 T; 0 other;
     cggacgcgtg ggtttagcca tggcagatga gaccttcaaa tacgtgattc ttggtggtgg        60
     cgttgccgct ggatatgcag ctagggaatt cgttaaacag ggacttaatc caggagaatt       120
     ggcgattata tccaaggagg cggttgctcc ttatgagcgt ccagctctta gcaaagccta       180
     tctctttccc gagtcccctg ctagattacc agggttccat gtatgtgtag gaagtggagg       240
     ggagaggttg cttcctgatt ggtacaagga aaaagggatc gagttgattc tcagtacaga       300
     aattgttgaa gcagatcttc ctgccaagag actcagaagt gctcatggga aaatttacaa       360
     ttaccaaact ttgattattg ctactggttc tactgttatt aaattgtctg actttggagt       420
     tcaaggagcg gatgccaaaa acattttcta tttgagagaa attgatgatg ctgatcagct       480
     agttgaagca atcaaagcaa aggaaaatgg aaaagtggtt gttgttggag gaggatacat       540
     aggccttgag cttggtgcag cgttgagaat aaacaacttt gatgtcagca tggtttatcc       600
     tgaaccatgg tgcatgccta ggctattcac tccagaaata gctgctttct atgaaggtta       660
     ttatgcacaa aaaggaatca caatcattaa aggaacagtc gctgtaggct tcacagttga       720
     tactaatgga gaggttaagg aagtgaaact taaagatggc agggtcttag aagctgacat       780
     tgttgttgtt ggtgttggtg cacggccact gacaagctta ttcaagggtc aaatcgtaga       840
     agaaaaagga ggcattaaga ctgatgaatt cttcaagacg agtgttcctg atgtatacgc       900
     ggtgggcgat gtggctactt ttcctttgaa gctttacaat gaactgagaa gagttgagca       960
     tgttgaccat tctcgcaaat cagcagagca agctgtgaag gccatcaagg cgagtgaaga      1020
     gggcaaagcc attgaggagt atgactatct tccatacttc tactctcgat cctttgattt      1080
     atcatggcag ttttacgggg ataatgttgg cgacgcagtc ttatttggag acaacagtcc      1140
     tgattcagct actcacaaat ttgggtcata ttggatcaaa gatgggaagg ttgtgggagc      1200
     tttcctagag agtgggagtc ctgaagaaaa caaggccatt gccaaagttg ctaggattca      1260
     accatcagtt gagagctcag atttgctcct taaagaaggc atctcctttg catccaaggt      1320
     ttaaaagtta catttcacca tttactgaat aaattcaagt gcttggactt aatatttgat      1380
     caacttttaa tttcatatat gattacttca tgtatgttat ttattggaaa gttgagctga      1440
     atctgaaaac aagttttatc aagtgtttca aataaagtgt attactttaa gatttcgtg       1499
//
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