 |
|
EBI DbfetchID D25413; SV 1; linear; genomic DNA; STD; PRO; 2678 BP. XX AC D25413; XX DT 19-APR-1994 (Rel. 39, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 6) XX DE Thermus thermophilus proC gene for pyrroline-5-carboxylate reductase, DE complete cds. XX KW . XX OS Thermus thermophilus OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus. XX RN [1] RP 1-2678 RA Hoshino T.; RT ; RL Submitted (22-NOV-1993) to the EMBL/GenBank/DDBJ databases. RL Takayuki Hoshino, University of Tsukuba, Institute of Applied Biochemistry; RL Tennohdai 1-1-1, Tsukuba, Ibaraki 305, Japan (Tel:81-298-53-6782, RL Fax:81-298-53-4605) XX RN [2] RP 1-2678 RA Hoshino T., Kosuge T., Hidaka Y., Tabata K., Nakahara T.; RT "Molecular cloning and sequence analysis of the proC gene encoding RT .DELTA.1-pyrroline-5-carboxylate reductase from an extremely thermophilic RT eubacterium Thermus thermophilus"; RL Cell. Mol. Biol. Res. 199:410-417(1994). XX DR StrainInfo; 125622; HB27. XX FH Key Location/Qualifiers FH FT source 1..2678 FT /organism="Thermus thermophilus" FT /strain="HB27" FT /mol_type="genomic DNA" FT /note="clones pUC-pro4+ and pUC-pro4,6+" FT /note="cloning vector: pUC19" FT /db_xref="taxon:274" FT CDS <1..496 FT /codon_start=2 FT /transl_table=11 FT /note="ORF (truncated)" FT /db_xref="GOA:Q56431" FT /db_xref="InterPro:IPR006027" FT /db_xref="UniProtKB/TrEMBL:Q56431" FT /protein_id="BAA05000.1" FT /translation="ILRLGALEWLEGKPDHARVSPWVEEAKRRYPGLAGLVNAVLRRLA FT PREAPECVRLSLPDWLCEAWRGFFGDVAFAEGFNEPAPLFVTAYREVDLRPGPVPGSYL FT WEGPKTDFPALGLQPENPASLFAAKLLEARPGERVLDLCGGAGLKAFYLAAQGARWSPT FT T" FT RBS 941..948 FT CDS 960..1745 FT /codon_start=1 FT /transl_table=11 FT /gene="proC" FT /product="pyrroline-5-carboxylate reductase" FT /EC_number="1.5.1.2" FT /db_xref="GOA:P54893" FT /db_xref="InterPro:IPR000304" FT /db_xref="InterPro:IPR004455" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P54893" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="BAA05001.1" FT /translation="MRLAFVGLGKMGRSILKGALERGFLRPEEVGVLGRTPERSRELAE FT PFGVRPLTRADLGMAERVLIAVQPRDFPALAPEIAHHRLGYISIMAGISTSVLARRLDN FT RRVVRAMPNLAVVIGESSTALTALKEAREAEDLAFARALFATVGDVYEIPEHLFDAFTG FT MSASAPAYLAVVAEALADAGVKMGMPRALALRLAADALAATGELLKGRHPAQVKDEVAS FT PGGTTIHGLHALEARAVRAAFYEAVEAATRRGHELGESE" FT RBS 1734..1738 FT CDS 1742..2377 FT /codon_start=1 FT /transl_table=11 FT /note="ORF" FT /db_xref="UniProtKB/TrEMBL:Q56432" FT /protein_id="BAA05002.1" FT /translation="MRPPHRLAPAWGPRQKAWVGILGGRRANKAGKKGMGVVARLLGLG FT LLLGLAWAQILPPEGGLYVYSDGTVQALEAVEGGYRLAYRRDGKVFREDRLRFGAEGIY FT LEGVALPKGFFPFAPPLLLYPKRLVLGASWSGNARFQEQRVALAVRVEGVEGVRVPAGR FT FNAYRLRVAFTTERGGADVKLLYLVPGLGVVAFQAGEGLVGLVRFSAP" FT repeat_region 1746..1781 FT /rpt_type=INVERTED FT /rpt_unit_range=1771..1781 FT /rpt_unit_range=1746..1756 FT RBS 1787..1790 FT RBS 1838..1841 FT CDS complement(2364..>2678) FT /codon_start=1 FT /transl_table=11 FT /note="ORF (truncated)" FT /db_xref="UniProtKB/TrEMBL:Q56433" FT /protein_id="BAA05003.1" FT /translation="RMAVGVFRGGRASTDLAMRLTPFLSGQRALRLEDLRSVPGLERAR FT YDLELLLTRHAKRAGWPVVYVPMEGVSQVMKEEKRGVLAGLGHRLRMYWEILRYRLRAR FT " XX SQ Sequence 2678 BP; 347 A; 961 C; 974 G; 396 T; 0 other; gatcctccgc ctgggggccc tggagtggct tgagggcaag ccggaccacg cccgggtgag 60 cccctgggtg gaggaggcga agcgccgcta cccgggcctc gctgggctgg tgaacgccgt 120 tttgcggcgc cttgccccta gggaggcccc ggagtgcgtg cgcctttccc tccccgactg 180 gctctgcgag gcctggcggg gcttcttcgg ggacgtggcc ttcgccgagg gcttcaacga 240 gcccgccccc ctcttcgtca ccgcctaccg ggaggtggac ctgaggcccg gccccgtccc 300 cgggagctac ctttgggaag gccccaagac cgactttccc gccctcggcc tccagccgga 360 aaaccccgcc tccctcttcg cggccaagct cctcgaggcc cgccctgggg agcgggtgct 420 ggacctttgc ggaggggccg ggctcaaggc cttctacctc gccgcccagg gggcgaggtg 480 gtctcctacg acctaaaccc caggcggcag gaggcggggg cgaggacggc gcggcggctt 540 ggcctttggg tccactaccg cacccaggac ctgacccggc cggtgcccga gcgggcgaag 600 aaggtcctcc tggacgcccc ctgcaccggg accggcacct tccgcgccca ccccgagctc 660 cgctaccgcc taagccccga ggaccctgcc cgcatggccg ccctccagct ccagctcctg 720 gagacggcgg cccaggccac ggaggaaggg ggggttctcg tctacagcgt ctgcaccctg 780 acggaggagg agggggaagg ggtggtgcgg gccttcctgg cgcgccaccc tgagttccgc 840 cccgagcccg tccaaccccc cttccccgtc ctcgcccggg ggcttggggt ctacgtggac 900 ccgaggggag ggctggacgg cttctactac gccaagctgc ggaaggtaaa ctctcaggca 960 tgaggctggc cttcgtgggt ctcggcaaga tgggccggag catcctcaag ggggctctgg 1020 agcggggctt cttgcgcccg gaggaggtgg gggtcttggg gcggacgccc gagcggagcc 1080 gggagctcgc cgagcccttc ggcgtccgcc ccttgacgcg ggcggacctg ggcatggccg 1140 agcgcgtcct gatcgcggtc cagccccggg actttcccgc cctggccccg gagatcgccc 1200 accaccgcct gggctacatc tccatcatgg cggggatctc cacctcggtc ctcgcccgca 1260 ggctggacaa ccgccgcgtg gtccgggcca tgcccaacct ggcggtggtc atcggggaga 1320 gctccaccgc cctcaccgcc ctaaaggagg ccagggaggc ggaagacctc gccttcgccc 1380 gggccctttt cgccacggtg ggggacgtgt acgagatccc cgagcacctc tttgacgcct 1440 tcaccggcat gtccgcctcc gcccccgcct acttggcggt ggtggccgag gccttggcgg 1500 acgcgggggt gaagatgggc atgccccggg ccctggccct ccgcctcgcc gccgacgccc 1560 tggcggccac gggggagctt ctcaagggca ggcaccccgc ccaggtcaag gacgaggtgg 1620 cgagcccggg gggaaccacc atccacggcc tccacgccct cgaggcccgg gcggtgcggg 1680 cggcctttta cgaggcggtg gaggcggcca cccgtagggg gcacgagctc ggcgagtcgg 1740 agtgaggcca ccccatcggc ttgcgcccgc atgggggccc cggcagaagg cttgggtggg 1800 gattttgggc ggcaggaggg cgaacaaagc gggaaagaag ggtatggggg tggtggcgcg 1860 gcttctcggc cttggcctcc tcctcggcct ggcctgggcc cagatcctcc cccctgaggg 1920 ggggctttac gtctacagcg acggcacggt ccaggcgctc gaggcggtgg aaggagggta 1980 ccgcctcgcc tacaggcggg acgggaaggt cttccgcgag gaccggctcc gcttcggcgc 2040 agagggaatc tacttggaag gggtcgcgct ccccaagggg ttcttcccct tcgccccgcc 2100 cttgctcctc tacccgaaga ggcttgtcct gggcgcttcc tggtcgggga acgcccgttt 2160 ccaggagcag cgggtggccc tggcggtccg ggtggagggg gtggaggggg ttagggtgcc 2220 cgcggggcgc ttcaacgcct accgcctgcg ggtggccttc accacggagc ggggcggggc 2280 cgacgtgaag ctcctctacc tcgtccccgg cctcggcgtg gtggccttcc aggcggggga 2340 gggcctcgtg ggcctcgtcc gcttcagcgc gccctgagcc ggtagcgcag gatctcccag 2400 tacatgcgga gccggtggcc gagcccggcg agaacccccc gcttctcctc cttcatcacc 2460 tggctcaccc cctccatggg cacgtacacc acgggccagc ccgcccgctt ggcgtgccgg 2520 gtgaggagga gctccaggtc gtagcgggcc ctttccaggc cgggcacgct cctcaggtcc 2580 tccaggcgga gggccctctg gcccgagaga aagggggtga ggcgcatggc caggtccgtg 2640 gaggcccgcc cccctcggaa gacccccacg gccatccg 2678 // |
|