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EBI DbfetchID D17399; SV 1; linear; genomic DNA; STD; PRO; 2211 BP. XX AC D17399; XX DT 29-SEP-1994 (Rel. 41, Created) DT 12-MAY-2009 (Rel. 100, Last updated, Version 15) XX DE Pseudoalteromonas piscicida cht60 gene for chitobiase 60 precursor, DE complete cds. XX KW . XX OS Pseudoalteromonas piscicida OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; OC Pseudoalteromonadaceae; Pseudoalteromonas. XX RN [1] RP 1-2211 RA Tsujibo H., Fujimoto K., Tanno H., Miyamoto K., Imada C., Okami Y., RA Inamori Y.; RT ; RL Submitted (11-AUG-1993) to the EMBL/GenBank/DDBJ databases. RL Contact:Hiroshi Tsujibo Osaka University of Pharmaceutical Sciences, RL Department of Microbiology; 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, RL Japan XX RN [2] RP 1-2211 RX DOI; 10.1016/0378-1119(94)90843-5 RX PUBMED; 8063094. RA Tsujibo H., Fujimoto K., Tanno H., Miyamoto K., Imada C., Okami Y., RA Inamori Y.; RT "Gene sequence, purification and characterization of RT N-acetyl-beta-glucosaminidase from a marine bacterium, Alteromonas sp. RT strain O-7"; RL Gene 146(1):111-115(1994). XX FH Key Location/Qualifiers FH FT source 1..2211 FT /organism="Pseudoalteromonas piscicida" FT /strain="O-7" FT /mol_type="genomic DNA" FT /clone="pCHT982" FT /note="originally submitted as 'Alteromonas sp. O-7'" FT /db_xref="taxon:43662" FT -35_signal 330..335 FT -10_signal 357..362 FT RBS 379..383 FT /note="putative" FT CDS 395..2191 FT /codon_start=1 FT /transl_table=11 FT /gene="cht60" FT /product="chitobiase 60 precursor" FT /EC_number="3.2.1.52" FT /db_xref="GOA:P48823" FT /db_xref="InterPro:IPR001764" FT /db_xref="UniProtKB/Swiss-Prot:P48823" FT /protein_id="BAA04223.1" FT /translation="MSFITSAHATAAQVPLTTSQMLGQKLMLDFRYYCGESKKPSGDCR FT AAMTTLPPELSELISRYDIGGAILFAENVQNTAQIISLTNALQSAAQQSKSQLPLFIAI FT DQEGGRVARINREQATSFTGNMSIGATYPKQGDIYATKVASAIGKELNSLGINVNFAPT FT VDVNSNPNNPVINVRSFSENPTVVTKLGLAQVKAFEAAGVLSALKHFPGHGDTHVDSHT FT GLPRVDHDRDKINQQDLLPFAEIIKASPPGMIMTAHIQYPALDNSKVVNSQGESMIRPA FT TMSYQIMTQLLRHELGYQGVTVTDALDMAGISDFFNPVDATIETFNAGVDIALMPIAIR FT NRADIKRFEQYMAQLADALETNKLNQEQLSSSMARIAKLKTKLPQSSASLAIANSTLGN FT PSHRRLEAELALAAITEVKNDGVLPLRDNAQVVHLIMPDRQKCFALEQALQTYSKNSLT FT LSCTSLQAYDPDIAHDAIKQADMIIAAHASPPQSAVEIGGMDDVKKLREHGVARNVQPA FT ALKALLQYGQQQGKKQLFISLRAPYEISTFGPLSNAVLASYAYNVDVNHDKKVAGPAYT FT ALAKVILGIAKAEGSLPVTVNH" FT sig_peptide 395..427 FT mat_peptide 428..2188 FT /gene="cht60" FT /product="chitobiase 60" FT /function="most active towards PNP-beta-GlcNac and FT diacetylchitobiose" FT /EC_number="3.2.1.52" FT /note="N-acetyl-beta-glucosaminidase" XX SQ Sequence 2211 BP; 680 A; 516 C; 486 G; 529 T; 0 other; gaattcggtt cattgtcgct cgacttgccg cctagtaatc taggcaaaag ttgagcaaca 60 atgaacaaag cgcactcaga tgaacccaaa gggcagcgct tgattagcat tttctctgtg 120 ttgccccaca aggatgtggg gtaaggtgac tttgcaagag cacaaagtct ttatcgccag 180 acttacatag aatgactatg ctacgctggc tccaccttgt ataaatacca atcaaactgc 240 tgcaaaaaca aacttgaaag atcaacaggc cctaatagaa agcactttgt agtcgcttct 300 aaatcaatgt taaagtgtag gcaattttat tcagcgttta ttattgattg tgcgattatt 360 atctctgctc actactttag gaactatcac attcatgagt ttcatcactt cagctcacgc 420 aacagcagca caagtgccac tgaccacttc gcaaatgctt ggacaaaagc ttatgctcga 480 tttccgctat tactgcggtg aaagcaaaaa gccaagtgga gattgtagag cagcgatgac 540 aactttaccg cctgagctgt ccgaattgat tagtagatac gacatcggcg gcgctatttt 600 gttcgccgaa aatgtgcaaa acaccgcgca aatcatcagc ctaaccaatg ctttgcaaag 660 tgcggcacaa caaagtaaaa gtcaacttcc tttatttatt gccatcgatc aagagggtgg 720 ccgtgttgcg cgaataaacc gtgaacaagc aacgtcgttt accggcaata tgagtattgg 780 agcaacctat ccaaaacagg gcgatattta cgcaaccaaa gtggcaagcg cgatcggaaa 840 ggagctaaat agcttaggga tcaacgtaaa cttcgctcca acggtggatg tgaacagcaa 900 cccgaacaat ccggtgatca atgtgcggtc attctcggaa aatccaacag tggtcaccaa 960 attaggtctt gctcaggtaa aagcatttga ggccgcaggt gtactttctg cgctaaagca 1020 ttttcctggc cacggtgata cacatgttga tagccacacg ggactacctc gtgttgatca 1080 cgaccgcgac aaaattaacc agcaagattt attacccttt gccgagataa tcaaagcatc 1140 gcctcctggc atgataatga ctgcccatat tcaatatccg gcacttgata acagtaaagt 1200 cgttaactct caaggtgaga gcatgataag gcctgcgact atgtcttatc agatcatgac 1260 acaactacta agacacgagt tgggatacca aggcgtgacc gtcaccgatg cgctagatat 1320 ggctgggatc agcgactttt ttaatccagt cgatgcaaca attgaaacgt ttaatgctgg 1380 cgtggatatc gcgctaatgc ctattgctat tcgcaatcgt gcagacatca aacgctttga 1440 gcaatatatg gctcagctag ctgacgcgct tgagaccaat aaactaaacc aagaacaact 1500 tagcagttct atggcgcgta tcgccaagct caagacaaag ctgccgcaat cgagcgcttc 1560 tttggccatt gccaattcaa cgctgggcaa tccaagccat cgtcgtttag aagcagagct 1620 tgcactagca gctatcaccg aagtaaaaaa cgatggtgta ttaccgctca gagataacgc 1680 acaagttgtt caccttatca tgcctgatag gcaaaaatgc tttgcgcttg aacaagcatt 1740 acaaacatac agcaaaaact cgctgacact ttcttgtaca agcctacaag cctacgaccc 1800 ggacattgca catgatgcaa ttaaacaggc cgacatgata atcgccgcac atgcatcacc 1860 accacaaagt gcagtagaaa ttggcggcat ggacgatgtg aaaaagttac gagagcatgg 1920 ggtggcgcgt aatgtacaac ccgcagcgct gaaagcatta ctgcaatacg gtcagcaaca 1980 gggtaaaaag cagctattta tcagcctaag agcaccatac gaaattagta cctttggtcc 2040 tcttagtaac gcagtattag caagttatgc ttataatgta gacgtaaatc atgataaaaa 2100 agtagctggg cctgcctaca cggcgctggc caaagtgatt ttgggaatag cgaaagcgga 2160 aggttcactg cctgttacag taaaccacta attggaggaa tctatggatc c 2211 // |
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