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EBI DbfetchID D17388; SV 1; linear; genomic DNA; STD; PRO; 4988 BP. XX AC D17388; XX DT 07-SEP-1993 (Rel. 36, Created) DT 21-JAN-1999 (Rel. 58, Last updated, Version 5) XX DE Haemophilus gallinarum genes for HgaI methylase 1, HgaI methylase 2, and DE HgaI endonuclease. XX KW HgaI endonuclease; HgaI methylase 1; HgaI methylase 2. XX OS Avibacterium paragallinarum OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Avibacterium. XX RN [1] RP 1-4988 RA Sugisaki H.; RT ; RL Submitted (09-AUG-1993) to the EMBL/GenBank/DDBJ databases. RL Hiroyuki Sugisaki, Institute for Chemical Research, Kyoto University; Uji, RL Kyoto 611, Japan (Tel:0774-33-0883(ex.2195), Fax:0774-33-1247) XX RN [2] RX PUBMED; 1856224. RA Sugisaki H., Yamamoto K., Takanami M.; RT "The HgaI restriction-modification system contains two cytosine methylase RT genes responsible for modification of different DNA strands"; RL J. Biol. Chem. 266(21):13952-13957(1991). XX RN [3] RP 1-4988 RA Sugisaki H.; RT "Nucleotide sequence of the gene of HgaI restriction endonuclease"; RL Bull. Inst. Chem. Res., Kyoto Univ. 71:338-342(1993). XX FH Key Location/Qualifiers FH FT source 1..4988 FT /organism="Avibacterium paragallinarum" FT /strain="NCTC3438" FT /mol_type="genomic DNA" FT /db_xref="taxon:728" FT CDS 207..1304 FT /codon_start=1 FT /transl_table=11 FT /product="HgaI methylase 1" FT /db_xref="GOA:P25282" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P25282" FT /protein_id="BAA04206.1" FT /translation="MINIDRLFMKKNIMGLSLFSSAGIGEYFLSRVGIDIIVANELIKK FT RADLYQKIYPNHKMVIGDIRDQRIFNKVLNIALTNQVDFLIASPPCQGMSVAGKNRDVS FT NMANDNRNYLIMYVIAMIKKLKPAYILIENVPFLLKLELYIDNKLTPIKNILEDEFGSE FT YHIHFDILDAADYGTPQRRKRAIIRLNKKGTIWNLPLKQNIVSVEQAIGNLPSIESGKH FT SGLKWHYGRGHTEQQIEWMKHTPTGKSAFENLVHYPRKANGEKVKGYHSSYRRIRWDEP FT APTITIRNDAISSQRNVHPGRPLLDGTYSDARVLSVLELMRLTGLPDNWEIPDDTPEIL FT IRQIIGECIPPLLIENITREIFNEN" FT CDS 1294..2370 FT /codon_start=1 FT /transl_table=11 FT /product="HgaI methylase 2" FT /db_xref="GOA:P25283" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P25283" FT /protein_id="BAA04207.1" FT /translation="MKINAMSLFSSAGIGELDLHKGNLNFVVANELLKKRADTYQFFYP FT ETKMFQGDISDEKLKREILLSAQQNNVKFLLATPPCQGLSSVGKNKHQDHFIKDNRNFL FT IFEVFEFIDVLNLDFILIENVPRFIEMYFPYNGQLLLLEEILKIKYASKYQIDIVILNA FT KDYGICQSRPRAIIKMYKYGITWKLPTIQAEISLQRAIGHLPPLEPGEVSSIKWHSAPN FT VKPSIIEAIRHTKPGTSAISNPIFYPKKDNGERIKGFHNTYKRMEWDKPAPARTTYSGS FT ISSHNNIHPGRLQLDGTYSDPRVLSLLETFIVSSIDENIEFPPGSSETYIRTIIGEAIP FT PKLLSAICFPDGENINVK" FT CDS 2345..3811 FT /codon_start=1 FT /transl_table=11 FT /product="HgaI endonuclease" FT /db_xref="GOA:P43418" FT /db_xref="UniProtKB/Swiss-Prot:P43418" FT /protein_id="BAA04208.1" FT /translation="MEKILMLNDDQIWIFKKHTNNIQLLIEVALYLKSNKSSVSKKDKD FT AMYDIFSESELYNPRESLRDKPLDTINHKLDGLSYFMFGYSDRINDENKFIFSPLGNLF FT LKYLHDKDKLSKIFSCMLISMQFPHPYSKPSECFLLYPFRLIFKLLLDKRLQGRLYHYE FT VYKIIIHTISIDEAKYEFLVKSILNSRKKSWNEKLNELSEIQHKVVKSVYEWQYYIVPL FT LGSLHIFKINNGDIEQKLYHPQKDGSKSPPTARKANNGYVEINDNLTNFIDKLLNKYSF FT LDTPILLSDSQRKSNDVTKEIYSFYPELLLAEIGETISFESHILNIPKLITEYSKNPDN FT STSGKFEKILEEAFNLFIDVEAQWLAGAGRTDIECMYLPINEKFSIEAKSTKNKLSMIN FT SGRLKRHRTLISANYTIVITPRYVPSVRYDIEAQDIVLITADTLAEYLYNNIISNNRDI FT SYADIQAIIVANLGKDISTQISNLTLSKFG" XX SQ Sequence 4988 BP; 1837 A; 725 C; 791 G; 1635 T; 0 other; agcttaaatg tatttggatt atccaacatc tatactgtct cttactctct atttaaatat 60 aaataaaatt atgtggtaca ttcaagttat taagtgatat tcatggtaaa taattcgaag 120 aactaaaacg agaaaatttg atcacaaaat aatagacaag attctacaaa taaaattgta 180 tgtaattaga tttaatcatt aatttcatga taaatataga taggttattt atgaaaaaaa 240 atataatggg attatctctt ttttctagtg caggaatagg agaatatttc ttatcgagag 300 ttggtattga tattatagtt gctaacgaat taataaaaaa aagagctgat ttatatcaaa 360 aaatttatcc aaaccataag atggtgatag gggatattag agatcaacgt atttttaata 420 aagtattaaa tattgccctt actaatcaag tagatttttt aattgcatct cccccttgtc 480 aaggtatgag tgtagcagga aaaaatcgag atgttagtaa tatggctaat gataacagaa 540 attatctcat tatgtatgtg atagcgatga taaaaaaact taagccagct tatattttaa 600 tagaaaatgt tccgttttta ttaaaattag agctttatat tgataataaa ttaacaccta 660 ttaagaatat tttagaagat gagtttggaa gcgaatacca tatacatttt gatattctag 720 atgctgcaga ctatgggacg ccacaaagaa gaaaaagggc tattattagg ctaaataaaa 780 aaggaacaat ttggaattta cctttaaaac aaaacatagt aagtgtggag caagcaatcg 840 gcaatttacc ttctattgag tcaggtaaac attcaggttt gaaatggcat tatggcaggg 900 ggcatacaga gcaacaaata gagtggatga agcatactcc tacaggaaaa tcagcatttg 960 agaatttagt tcattatcct agaaaagcaa atggtgaaaa agttaaagga tatcactcta 1020 gttatcgtag aattcgatgg gatgaaccag ctccaacaat aactattcgt aatgatgcaa 1080 ttagctctca acgtaatgtt catccaggta gaccgttatt agatggtaca tattctgatg 1140 ctagagtatt atctgtttta gaattaatga gattaacagg gttacctgat aattgggaaa 1200 tcccagatga tactccagaa atattgatta gacagatcat tggagaatgt atccctccat 1260 tacttataga aaatataact agggaaatat ttaatgaaaa ttaatgcaat gtcgcttttt 1320 tctagtgcag gaattggtga gttagatctc cataaaggaa atctaaattt tgttgttgct 1380 aatgaattac ttaaaaaacg tgcggacact tatcaattct tttatccaga aacaaaaatg 1440 tttcaaggtg atatctcaga cgaaaaatta aaacgtgaga tacttctttc cgctcaacaa 1500 aataatgtaa aatttttatt agcaacacca ccttgtcaag gattaagttc agtaggaaaa 1560 aataaacatc aagatcattt tattaaagat aatcgtaatt ttctaatttt tgaagtattt 1620 gaatttattg atgttcttaa tttggatttt atcctaattg agaatgttcc tagattcatt 1680 gagatgtatt tcccatataa tggacaatta ttacttctag aagaaattct aaaaataaaa 1740 tatgcttcaa aatatcaaat tgatattgtt attttaaatg caaaagatta tggaatttgt 1800 cagtcaagac ctagagctat aataaaaatg tataaatatg gtattacgtg gaaactacct 1860 actatacaag cagaaatttc tttacagcga gcaatagggc atttaccgcc gcttgaacca 1920 ggggaagttt catctatcaa atggcattct gctcctaatg taaaaccatc tatcatagaa 1980 gctatccgtc atacaaaacc aggaacaagt gctattagta atcctatttt ttacccaaag 2040 aaagataatg gggaaagaat taaaggtttt cacaatactt ataaaagaat ggaatgggat 2100 aaaccagctc cagcaagaac tacatatagt ggtagtatta gttcacataa caatattcac 2160 ccaggtagat tacaattaga cggtacttat tctgatccta gagtgttaag tttattggaa 2220 actttcattg tgagttctat cgatgaaaat atagaatttc caccaggctc cagtgagact 2280 tatattagaa ctattattgg tgaggctatt ccacccaaat tactttcagc aatatgtttt 2340 cctgatggag aaaatattaa tgttaaatga tgatcaaatt tggatattta aaaaacatac 2400 taataatatt cagctattaa ttgaagttgc tttgtattta aaaagcaata aatcttctgt 2460 ttcaaaaaaa gataaagatg cgatgtatga tatattcagt gaatctgaat tatataatcc 2520 aagagaatct ttacgtgata aacctttgga cacaattaat cataaattag atgggttatc 2580 ttattttatg tttgggtact cagatagaat aaatgatgaa aataaattta tttttagtcc 2640 attaggaaat cttttcctga aatatctaca tgataaagat aaattaagca aaattttttc 2700 ttgtatgctt attagtatgc agtttcctca tccatatagt aagcctagtg aatgcttttt 2760 actatatcca tttagattaa tttttaaact gcttttagat aaacgcctac aaggtaggct 2820 atatcattat gaagtatata aaattattat tcatacaata tctatagatg aagccaaata 2880 tgaatttcta gtcaagagta tattaaattc tagaaaaaaa tcttggaatg agaaacttaa 2940 tgaattatca gaaatacaac ataaagttgt taaatctgta tatgagtggc aatactacat 3000 tgtgccattg ttaggtagct tacacatttt taaaattaat aatggagata tagagcaaaa 3060 actttatcat cctcaaaaag atggaagtaa atcacctcca acagcaagaa aggcaaataa 3120 tggctatgta gaaattaatg ataatttgac taattttatt gataaattat tgaacaagta 3180 ttctttttta gataccccta tcttattatc tgatagtcaa cgaaaatcaa atgatgtgac 3240 taaggaaatt tattcttttt acccagagct tcttttagca gaaattggcg aaacaatctc 3300 ttttgaatct catattttaa atattcctaa attaatcaca gaatattcca agaatccaga 3360 taatagcaca tctggcaaat ttgagaaaat tcttgaggaa gcttttaatt tattcattga 3420 tgtagaagct caatggttag caggagcagg cagaacagat attgaatgta tgtatttgcc 3480 aataaatgaa aaattttcta ttgaagctaa atcaacaaaa aataaattaa gtatgattaa 3540 ttcaggtaga ttaaaacgcc acagaacttt aattagtgca aattatacta ttgttattac 3600 tccaagatat gtacctagtg tacgttatga tattgaagca caagatatag ttctcataac 3660 agccgataca ttagcagagt atttatacaa taatattatt tctaataatc gagatatttc 3720 ttatgctgat atacaagcta tcattgttgc taatttggga aaagatatta gtactcaaat 3780 ttcaaattta acactttcca aatttggttg atggataaat gaatatgcat aagaacatta 3840 cagaaaaaac tattgtatga tagcaatcac aaaattgtga tctacatacc tcataactta 3900 tacagtttta aaagggttaa atcgccattc ctatattcga gtgggcgatt ttttatgtct 3960 agcgtttagc tttgagtacc ttgtacacac aaggaatact tatggatcat tacttacttt 4020 caagcaaatc taaaacatta tcccttaaac atatcttccg tctaagtgaa gatgaagcct 4080 tccaactttt gaaagcaaac cgttgggaaa atcctgataa aatcctccaa agtagtacgt 4140 ctaaattata gaataagcca cttaagataa tcactcaagg tgtttaaaat atcatcaatt 4200 aaacttgtca aaaaatttct cttgacagaa ctaagacctt tttggcactc gctatattca 4260 aaaatgcaga aaatttcaaa aagcacttga caaaagaaaa cgcttgacaa gcaccctata 4320 aaataatatc tttttgccca ccgagaatag tcccttcatt atgactttgt tttttaagcc 4380 taaaaaggct tggaaatttc agagttttaa tgaagggatt tttgtttcta tggcacaaca 4440 ttttcggtta tcagcgacag cacgccagct atctatggat agcttggtga gcttgtctga 4500 tgacgagatt tttcagctat tgaaacaagc tcgatggggt aatgtagatg aaataaacga 4560 tgttatttgt ccgcattgcc atactcgcca ccacgcctat ttcatttcta ctcgtaaaca 4620 atggcagtgc aaacattgcc aacatcgttt ttctattaca gcaggcacta tttttcaagg 4680 tgcaaaactt tctttgcgta aaattgtatt ggcaattttc tatttttcga ctgaaagcaa 4740 aggattatct gctattacac tatcacacaa gctcaacgtg caatataaaa cagcgtgggt 4800 attgctacat aaatttcgtg aatcgcttga taaagccaaa gatttaacac atttaagtgg 4860 cgaagttcat attgatggcg cttacatgaa caactacatt cgtcctaaaa actttctcca 4920 taaacgtatt gatagatgaa agaaacgtta tcagcgtgca gataaatctt gtgtattggt 4980 attccgtc 4988 // |
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