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EBI DbfetchID D17366; SV 1; linear; genomic DNA; STD; PRO; 6140 BP. XX AC D17366; XX DT 09-FEB-1995 (Rel. 42, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 6) XX DE Staphylococcus aureus atl gene for autolysin, complete cds and other ORFs. XX KW atl; autolysin; endo-beta-N-acetylglucosaminidase; KW N-acetylmuramoyl-L-alanine amidase. XX OS Staphylococcus aureus OC Bacteria; Firmicutes; Bacillales; Staphylococcus. XX RN [1] RP 1-6140 RA Oshida T.; RT ; RL Submitted (09-AUG-1993) to the EMBL/GenBank/DDBJ databases. RL Tadahiro Oshida, Pharmacological Research Lab. Tanabe Seiyaku Co., LTD.; RL 2-2-50 Kawagishi, Toda, Saitama 335, Japan (Tel:048-433-2743, RL Fax:048-433-2735) XX RN [2] RP 1-6140 RX DOI; 10.1073/pnas.92.1.285 RX PUBMED; 7816834. RA Oshida T., Sugai M., Komatsuzawa H., Hong Y.M., Suginaka H., Tomasz A.; RT "A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine RT amidase domain and an endo-beta-N-acetylglucosaminidase domain: cloning, RT sequence analysis, and characterization"; RL Proc. Natl. Acad. Sci. U.S.A. 92(1):285-289(1995). XX FH Key Location/Qualifiers FH FT source 1..6140 FT /organism="Staphylococcus aureus" FT /strain="RN450" FT /mol_type="genomic DNA" FT /clone="pAT8, pAT40, pAT37" FT /db_xref="taxon:1280" FT CDS <1..641 FT /codon_start=3 FT /transl_table=11 FT /product="ORF1" FT /db_xref="InterPro:IPR004474" FT /db_xref="UniProtKB/Swiss-Prot:P52078" FT /protein_id="BAA04182.1" FT /translation="YQKLNGDEALAVARTRHHDSDLKRGQRQMELIKILFQKAQEVDSI FT DKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIKTAQLKGKDDMLNGIYYYHPSV FT ESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSLIPLTPLDNSLLRKEQNDTTDKD FT KTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQAPQASNNQNGVVN" FT RBS 675..680 FT CDS 689..1159 FT /codon_start=1 FT /transl_table=11 FT /product="ORF2" FT /db_xref="UniProtKB/Swiss-Prot:P0A0Q4" FT /protein_id="BAA04183.1" FT /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHRE FT NYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMKSL FT SIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL" FT terminator 1175..1212 FT -35_signal 1248..1253 FT -10_signal 1271..1276 FT RBS 1303..1309 FT CDS 1314..1748 FT /codon_start=1 FT /transl_table=11 FT /product="ORF3" FT /db_xref="GOA:P0A0M9" FT /db_xref="InterPro:IPR000182" FT /db_xref="UniProtKB/Swiss-Prot:P0A0M9" FT /protein_id="BAA04184.1" FT /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHLI FT GYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVATM FT NAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN" FT terminator 1760..1801 FT -35_signal 1878..1883 FT -35_signal 1896..1901 FT -10_signal 1901..1906 FT -10_signal 1919..1923 FT RBS 1960..1964 FT CDS 1976..5746 FT /codon_start=1 FT /transl_table=11 FT /gene="atl" FT /product="autolysin" FT /note="amidase domain: nt2588..4300, glucosaminidase FT domain: nt4301..5743." FT /db_xref="GOA:Q2FZK7" FT /db_xref="InterPro:IPR002901" FT /db_xref="UniProtKB/Swiss-Prot:Q2FZK7" FT /protein_id="BAA04185.1" FT /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLDS FT NKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVNGD FT TRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKSAAK FT PAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVN FT SSINDYICKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYM FT KNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNN FT YADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQL FT YDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGVAQIKPTNS FT GLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNTA FT KSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYGSV FT NGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTTVYD FT KTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPVNVMQ FT TYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVNGKSGWVS FT KAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRASVYEKTAKN FT GAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVKTTQKYTV FT NKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNRTGWVNAKD FT LTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQPFAVVKEQVI FT NGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQYKPQVQRVPGK FT WTDAKFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKGKGVLENQGAAF FT NKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTKYHNVFGIAAYDN FT DPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRWNPAHPGTHQYATD FT VDWANINAKIIKGYYDKIGEVGKYFDIPQYK" FT terminator 5761..5810 FT terminator 5826..5888 XX SQ Sequence 6140 BP; 2345 A; 1028 C; 1006 G; 1761 T; 0 other; ggtaccaaaa attaaatggt gatgaggctt tagcagttgc tagaactaga catcatgatt 60 cagacttaaa acgtggacaa cgtcaaatgg aattaattaa gattttattc caaaaagcac 120 aggaagttga ttctattgat aaacttgata acgtgattca aattgtaggt aaaaatgcaa 180 agcataattt aactaactct gaaattaaag ctttagcaaa aatgtactta acaaatgatg 240 ttgaaattaa aactgcgcaa ttaaaaggta aagatgatat gttaaatggt atttactatt 300 atcatccaag tgttgaaagc attcaaaaat atgcaaactt acttcgtaaa gacttagaat 360 tatcacctat taatgataaa aatgatttct tagatcaacg tgtcattaat cattatggtt 420 cattaatacc attaacgcct ttagataata gtttattgag aaaagaacaa aatgatacga 480 cagataaaga taagacatct aacgagaaca gtgattcaac taataacagt gattccagca 540 atcaacaaca acctgctaca gatcaaaact caaatcaaaa tcaaggtggc acacaacaag 600 cgccacaagc ttcaaataac caaaatggtg ttgtaaatta atattatatg acgattaaca 660 tttactattt atgaaaggag catcatacat gagtagaaaa acatacgaaa agattgcaaa 720 tataaatggc atgtttaata tgttagaaca acaaatcatt catagccaag atatggctca 780 ttttagaagt gaattttttt acgtcaatca tgagcatcga gaaaactatg aagcactcct 840 aatttattac aaaaatagta tcgacaatcc tattgtagat ggtgcatgtt atattttagc 900 cctacctgaa attttcaata gtgttgatgt tttcgaatca gagttaccat tttcatgggt 960 atatgatgaa aatggcatta ccgaaacaat gaaatcactt agcattccat tacaatattt 1020 agttgcagca gctttagaag taactgatgt gaatatattt aagccttcag gatttacaat 1080 gggaatgaat aattggaata ttgctcaaat gcgaatcttt tggcaatata cagcaattat 1140 tagaaaagaa gcactataac attaataatt aattagctat aaagatgatt cacaacaatc 1200 atctttatag cttttttatg tctaattatt tttgaggaaa ataacaatcg ttattctatg 1260 ttattattag taaattaaat gtaaatgatt ttgttaggat gtggaggaat tttatgtttt 1320 caaaagtaaa caatcaaaag atgttagaag attgcttcta tataagaaag aaagtgtttg 1380 tagaagaaca aggcgtccct gaggaaagtg aaattgatga atatgaatct gaatctattc 1440 acctcattgg atatgataat ggacagccag ttgccactgc tcgaatacgc cctattaatg 1500 aaacaactgt caaaatagaa cgagtagctg tgatgaaatc acatcgtgga caaggaatgg 1560 gtagaatgct tatgcaagct gtagaatcat tagctaaaga tgaaggtttt tacgtagcta 1620 ctatgaatgc ccaatgtcat gctatcccat tttatgaaag tttaaacttt aaaatgagag 1680 gtaatatatt tcttgaggaa ggcatcgagc atattgaaat gacaaaaaag ttaacctcgc 1740 ttaattaaaa aaagttgtat ctattttaga aacatttgta ataaaccaat tcttaaattg 1800 acatcaattc tatttgattt gtcacgtcac cattgagatt caaaattttt ttcatttttt 1860 tacagtgaaa atgtaaatta agagtatatt acaaattggt taaatacgca caggtatata 1920 aaacaggtac tataatgtta gtaataatta ataaatgtta ggagtaataa atagaatggc 1980 gaaaaaattc aattacaaac taccatcaat ggttgcatta acgcttgtag gttcagcagt 2040 cactgcacat caagttcaag cagctgagac gacacaagat caaactacta ataaaaacgt 2100 tttagatagt aataaagtta aagcaactac tgaacaagca aaagctgagg taaaaaatcc 2160 aacgcaaaac atttctggca ctcaagtata tcaagaccct gctattgtcc aaccaaaaac 2220 agcaaataac aaaacaggca atgctcaagt aagtcaaaaa gttgatactg cacaagtaaa 2280 tggtgacact cgtgctaatc aatcagcgac tacaaataat acgcagcctg ttgcaaagtc 2340 aacaagcact acagcaccta aaactaacac taatgttaca aatgctggtt atagtttagt 2400 tgatgatgaa gatgataatt cagaaaatca aattaatcca gaattaatta aatcagctgc 2460 taaacctgca gctcttgaaa cgcaatataa aaccgcagca cctaaagctg caactacatc 2520 agcacctaaa gctaaaactg aagcgacacc taaagtaact acttttagcg cttcagcaca 2580 accaagatca gttgctgcaa caccaaaaac gagtttgcca aaatataaac cacaagtaaa 2640 ctcttcaatt aacgattaca tttgtaaaaa taacttaaaa gcacctaaaa ttgaagaaga 2700 ttatacatct tacttcccta aatacgcata ccgtaacggc gtaggtcgtc ctgaaggtat 2760 cgtagttcat gatacagcta atgatcgttc gacgataaat ggtgaaatta gttatatgaa 2820 aaataactat caaaacgcat tcgtacatgc atttgttgat ggggatcgta taatcgaaac 2880 agcaccaacg gattacttat cttggggtgt cggtgcagtc ggtaacccta gattcatcaa 2940 tgttgaaatc gtacacacac acgactatgc ttcatttgca cgttcaatga ataactatgc 3000 tgactatgca gctacacaat tacaatatta tggtttaaaa ccagacagtg ctgagtatga 3060 tggaaatggt acagtatgga ctcactacgc tgtaagtaaa tatttaggtg gtactgacca 3120 tgccgatcca catggatatt taagaagtca taattatagt tatgatcaat tatatgactt 3180 aattaatgaa aaatatttaa taaaaatggg taaagtggcg ccatggggta cgcaatctac 3240 aactacccct actacaccat caaaaccaac aacaccgtcg aaaccatcaa ctggtaaatt 3300 aacagttgct gcaaacaatg gtgtcgcaca aatcaaacca acaaatagtg gtttatatac 3360 tactgtatac gacaaaactg gtaaagcaac taatgaagtt caaaaaacat ttgctgtatc 3420 taaaacagct acattaggta atcaaaaatt ctatcttgtt caagattaca attctggtaa 3480 taaatttggt tgggttaaag aaggcgatgt ggtttacaac acagctaaat cacctgtaaa 3540 tgtaaatcaa tcatattcaa tcaaacctgg tacgaaactt tatacagtac cttggggtac 3600 atctaaacaa gttgctggta gtgtgtctgg ctctggaaac caaacattta aggcttcaaa 3660 gcaacaacaa attgataaat caatttattt atatggctct gtgaatggta aatctggttg 3720 ggtaagtaaa gcatatttag ttgatactgc taaacctacg cctacaccaa cacctaagcc 3780 atcaacacct acaacaaata ataaattaac agtttcatca ttaaacggtg ttgctcaaat 3840 taatgctaaa aacaatggct tattcactac agtttatgac aaaactggta agccaacgaa 3900 agaagttcaa aaaacatttg ctgtaacaaa agaagcaagt ttaggtggaa acaaattcta 3960 cttagttaaa gattacaata gtccaacttt aattggttgg gttaaacaag gtgacgttat 4020 ttataacaat gcaaaatcac ctgtaaatgt aatgcaaaca tatacagtaa aaccaggcac 4080 taaattatat tcagtacctt ggggcactta taaacaagaa gctggtgcag tttctggtac 4140 aggtaaccaa acttttaaag cgactaagca acaacaaatt gataaatcta tctatttatt 4200 tggaactgta aatggtaaat ctggttgggt aagtaaagca tatttagctg tacctgctgc 4260 acctaaaaaa gcagtagcac aaccaaaaac agctgtaaaa gcttatactg ttactaaacc 4320 acaaacgact caaacagtta gcaagattgc tcaagttaaa ccaaacaaca ctggtattcg 4380 tgcttctgtt tatgaaaaaa cagcgaaaaa cggtgcgaaa tatgcagacc gtacgttcta 4440 tgtaacaaaa gagcgtgctc atggtaatga aacgtatgta ttattaaaca atacaagcca 4500 taacatccca ttaggttggt tcaatgtaaa agacttaaat gttcaaaact taggcaaaga 4560 agttaaaacg actcaaaaat atactgttaa taaatcaaat aacggcttat caatggttcc 4620 ttggggtact aaaaaccaag tcattttaac aggcaataac attgctcaag gtacatttaa 4680 tgcaacgaaa caagtatctg taggcaaaga tgtttattta tacggtacta ttaataaccg 4740 cactggttgg gtaaatgcaa aagatttaac tgcaccaact gctgtgaaac caactacatc 4800 agctgccaaa gattataact acacttatgt aattaaaaat ggtaatggtt attactatgt 4860 aacaccaaat tctgatacag ctaaatactc attaaaagca tttaatgaac aaccattcgc 4920 agttgttaaa gaacaagtca ttaatggaca aacttggtac tatggtaaat tatctaacgg 4980 taaattagca tggattaaat caactgattt agctaaagaa ttaattaagt ataatcaaac 5040 aggtatgaca ttaaaccaag ttgctcaaat acaagctggt ttacaatata aaccacaagt 5100 acaacgtgta ccaggtaagt ggacagatgc taaatttaat gatgttaagc atgcaatgga 5160 tacgaagcgt ttagctcaag atccagcatt aaaatatcaa ttcttacgct tagaccaacc 5220 acaaaatatt tctattgata aaattaatca attcttaaaa ggtaaaggtg tattagaaaa 5280 ccaaggtgct gcatttaaca aagctgctca aatgtatggc attaatgaag tttatcttat 5340 ctcacatgcc ctattagaaa caggtaacgg tacttctcaa ttagcgaaag gtgcagatgt 5400 agtgaacaac aaagttgtaa ctaactcaaa cacgaaatac cataacgtat ttggtattgc 5460 tgcatatgat aacgatcctt tacgtgaagg tattaaatat gctaaacaag ctggttggga 5520 cacagtatca aaagcaatcg ttggtggtgc taaattcatc ggcaactcat atgtaaaagc 5580 tggtcaaaat acactttaca aaatgagatg gaatcctgca catccaggaa cacaccaata 5640 tgctacagat gtagattggg ctaacatcaa tgctaaaatc atcaaaggct actatgataa 5700 aattggcgaa gtcggcaaat acttcgacat cccacaatat aaataagcaa catgaacata 5760 ggatcaaaag tcatccccca ctatcaatca tgggggatga cctttgatcc cttttttata 5820 catacacaag caaaaatagc ggtgattgtt taccatcaat tttaacaatc accgctactt 5880 ttgcttgtaa ttcatgattc aatttttgtt gtgtgcacaa cgacactaaa ttatgtgttt 5940 gctattgtcg tgttacaacg atatgcgtcg ttgatttaac ttatcaagta attgatttaa 6000 attgtctaat tcgacttccg ataaacattg acatcttgct tcaatcaatt cgcaacgtgc 6060 attatttatt tgtgaaatta atgtacgtgc ttgatcagtc aaaattaatt ctttacatct 6120 taaatcttcg ctagattgtt 6140 // |
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