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EBI DbfetchID D14071; SV 1; linear; genomic DNA; STD; PRO; 3410 BP. XX AC D14071; XX DT 16-FEB-1994 (Rel. 38, Created) DT 12-MAY-2009 (Rel. 100, Last updated, Version 9) XX DE Methylobacillus glycogenes hom, thrC, thyA genes for homoserine DE dehydrogenase, threonine synthase, thymidylate synthase, complete and DE partial cds. XX KW . XX OS Methylobacillus glycogenes OC Bacteria; Proteobacteria; Betaproteobacteria; Methylophilales; OC Methylophilaceae; Methylobacillus. XX RN [1] RP 1-3410 RA Motoyama H.; RT ; RL Submitted (18-JAN-1993) to the EMBL/GenBank/DDBJ databases. RL Contact:Hiroaki Motoyama Kyowa Hakko Kogyo Co., Ltd., Tokyo Research RL Laboratories; 3-6-6 Asahimachi, Machida, Tokyo 194, Japan XX RN [2] RP 1-3410 RX PUBMED; 8117070. RA Motoyama H., Maki K., Anazawa H., Ishino S., Teshiba S.; RT "Cloning and nucleotide sequences of the homoserine dehydrogenase genes RT (hom) and the threonine synthase genes (thrC) of the gram-negative obligate RT methylotroph Methylobacillus glycogenes"; RL Appl. Environ. Microbiol. 60(1):111-119(1994). XX FH Key Location/Qualifiers FH FT source 1..3410 FT /organism="Methylobacillus glycogenes" FT /strain="ATCC 21371" FT /mol_type="genomic DNA" FT /clone_lib="DH5alpha" FT /note="clones pTK-1 and pTTS-1" FT /db_xref="taxon:406" FT promoter 399..405 FT CDS 491..1729 FT /codon_start=1 FT /transl_table=11 FT /gene="hom" FT /product="homoserine dehydrogenase" FT /EC_number="1.1.1.3" FT /db_xref="GOA:P37144" FT /db_xref="InterPro:IPR019811" FT /db_xref="UniProtKB/Swiss-Prot:P37144" FT /protein_id="BAA40415.1" FT /translation="MKPINVGLLGIGTVGGGTYTVLTRNQEEIARRAGRPIAITRVADR FT NLELARQVTGGKIDVTDDAFAIVSDPAIDIVVELIGGYTVARELVLKAIENGKHVVTAN FT KALIACMAMKFLPLRRKKASSSLLKLPLLVVSPLFKAVREGLAANRIEWIAGIINGTTN FT FILSEMREKGLAFADVLKEAQRLGYAEADPTFDVEGIDAAHKLMILAAMLWLFVHSLCR FT GITKLDAVDITKRTDKGVELRVHPTLIPEKRLICQCEWRNECCAGQGRCCWPTLYYGAG FT AGAEPTASAVADLVDGTDRGISCPHLAFQPDRLVDLPILPIGEISSAYYLRLRAVDKPG FT VLADVTRILGDRQISIDAMIQKEPQEGEDQADIIILTHVTVEKNMDDAIAAIEALPAIS FT GKVTRLRMEELSR" FT CDS 1779..3206 FT /codon_start=1 FT /transl_table=11 FT /gene="thrC" FT /product="threonine synthase" FT /EC_number="4.2.3.1" FT /db_xref="GOA:P37145" FT /db_xref="HSSP:1KL7" FT /db_xref="InterPro:IPR004450" FT /db_xref="UniProtKB/Swiss-Prot:P37145" FT /protein_id="BAA40416.1" FT /translation="MKYISTRGQSPALSFSEILLGGLAPDGGLYLPEQYPQFSADALSA FT MRGMNYRDLAFTILSRLIDDIPADDLRIIVDKTYRADVYAYARPGQDAEDITPTYKLED FT DLYLLSLSNGPTLAFKDMAMQLLGNLFEYVLAQKGETTNILGATSGDTGSAAEYAMRGK FT QGVKVFMLSPHQKMSRFQTAQMFSLQDDNIFNIAVKGVFDDCQDIVKAVSNDHAFKAKN FT KIGAVNSINWARVAAQVVYYFKGYFAVTADNAQQVSFAVPSGNFGNVCAGHIARMMGLP FT IAKLVVATNENDVLDEFFKTGVYRPRGSANTYHTSSPSMDISKASNFERFVFDLVGRDA FT AKVRELWGKVDAGGSFDLNDGGWFAKVADYGFVSGSSNHANRMQTIKATHERYGVTIDT FT HTADGLKVALEHREAGTPMLVLETALPAKFEDAIVEALGHKPERPHSLEGLESLPQRFE FT VMEADAAVIKQFIVEHI" FT CDS 3219..>3410 FT /codon_start=1 FT /transl_table=11 FT /gene="thyA" FT /product="thymidylate synthase" FT /EC_number="2.1.1.45" FT /db_xref="GOA:Q59525" FT /db_xref="HSSP:1TYS" FT /db_xref="InterPro:IPR000398" FT /db_xref="UniProtKB/TrEMBL:Q59525" FT /protein_id="BAA03157.1" FT /translation="MKVYHDLMRHVLEHGHKKEDRTGTGTLSVFGYQMRFDLAEGFPLL FT TTKKVHLKSIIHELLWFLQ" XX SQ Sequence 3410 BP; 755 A; 924 C; 958 G; 773 T; 0 other; cccgggcaat tggctatcca gaccgcgctt ggtggttacc agagcatcga tgacctggtg 60 gcaccgactg ggcgcttgtg caagcagcgc gaccttgcct acaagatgtt gacagatatt 120 ccaggggtta gctgtgtcaa gcccaaggct ggcctgtact tgttcccgcg ccttgacccc 180 aagatctacc cgattgagga cgaccagcag ttcatcctgg atttgttgct ggaagaaaag 240 tattgctggt gcaggctcag gtttaattgg catgctccga ccacttccgc gtggtgttcc 300 tgccgaatgt ggatgatctc accgaggcat tcacccggat tgcccgcttc ctggaaaatt 360 atcgcaagca gcacagcacg cttacggtgg tggatggcaa agcggcaaaa gaaatacaaa 420 caaagccaca caatgcaact gcatgatatg gctggtctat aagattgaaa agcaagaaac 480 tggaaactga atgaaaccca tcaatgttgg cctgctcggc atcggtactg tcggtggcgg 540 cacctatact gttttaaccc gtaaccagga agaaatcgca cgccgtgctg gacgcccaat 600 cgccattacc cgtgttgccg atcgtaatct ggagctggct cgccaggtga ctggtggaaa 660 aattgatgtc accgatgatg cttttgccat cgtgtctgat ccggcaattg atattgttgt 720 tgaactgatc ggtggctaca ccgtggcgcg tgaactggtg ctgaaggcca ttgagaatgg 780 caagcacgtg gtcacggcca ataaggcctt gattgcctgc atggcaatga aatttttgcc 840 gctgcgcaga aaaaaggcgt catcgtcgct tttgaagctg ccgttgctgg tggtatcccc 900 attattcaag gccgtacgtg aaggcctggc ggccaatcgt attgagtgga ttgctggcat 960 catcaatggc acgaccaatt tcattctctc ggaaatgcgt gaaaagggtc tggcgtttgc 1020 tgatgtgctt aaggaagctc aacgcctggg ttatgccgag gcagacccga ctttcgatgt 1080 cgaaggcatt gatgctgcgc acaagctcat gatccttgct gcgatgcttt ggctattcgt 1140 gcacagctta tgtcgaggca tcacgaagct ggatgccgtg gatatcacca agcgtaccga 1200 taagggcgtg gagttgcgtg tgcacccaac cttgatcccg gaaaagcgct tgatttgcca 1260 atgtgaatgg cgcaatgaat gctgtgctgg tcaagggcga tgctgttggc ctaccttgta 1320 ttatggtgcc ggtgctggtg cagaacctac cgctagtgcg gttgccgact tggtcgatgg 1380 taccgaccgt ggcatcagct gtccacacct ggctttccag ccagaccgct tggtagactt 1440 gcccatcctg cctatcggcg agattagcag tgcctattac ctgcgcctgc gtgcagtgga 1500 taagccaggc gtgctcgctg acgtgacccg tatcctcggt gaccgtcaga tttcgatcga 1560 tgcgatgatc cagaaagagc cacaagaggg cgaagaccag gccgacatca tcattctcac 1620 ccacgtcaca gttgagaaaa acatggatga tgccatcgcc gccattgagg cactacctgc 1680 catttccggc aaggtcacgc gtttgcgcat ggaagaacta agccgataac ttgagaaaag 1740 ggttgcaggc aggcctattg cctcctgcat cagttgcaat gaaatacatt tccacccgcg 1800 ggcaatcgcc tgcactgtct ttctctgaaa ttctgcttgg cggcttggcg cctgatggcg 1860 gcttgtattt gcccgagcaa tacccgcagt ttagcgctga cgcactgagc gcgatgcgcg 1920 gcatgaatta ccgcgacctg gcgttcacca tcctctcgcg tctgatcgac gatattcccg 1980 ctgacgacct gcgcatcatc gtcgacaaga cctatcgcgc ggatgtatat gcctatgctc 2040 gcccgggcca ggatgccgaa gacattacgc cgacctataa gctggaggac gacctctacc 2100 tgctttccct gtccaatggc ccaaccctgg cgttcaagga tatggcgatg cagttattgg 2160 gcaacctgtt cgaatacgtg ttggcgcaaa agggcgagac gactaatatt ctcggcgcga 2220 cctccggcga taccggttct gcggcggaat acgccatgcg cggcaagcag ggcgtcaagg 2280 tgttcatgct ctcgccgcac cagaagatga gccgtttcca gaccgcacag atgttcagcc 2340 tgcaagacga caatatcttc aatatcgcgg tcaagggcgt gtttgacgac tgccaggaca 2400 tcgtcaaggc cgtgtcgaac gaccatgctt tcaaggccaa gaataagatc ggtgccgtga 2460 attccatcaa ctgggcacgc gtggctgctc aggtagtgta ttacttcaag ggttatttcg 2520 ccgtcactgc ggataatgcg cagcaggtca gctttgccgt gccttccggc aacttcggca 2580 atgtctgtgc cgggcatatc gcccgcatga tgggcttgcc gattgccaag ctggtggtag 2640 cgaccaacga aaacgacgtg ctggatgagt tcttcaaaac tggcgtctac cgtccgcgcg 2700 gctccgccaa tacctaccat acttccagcc cctccatgga tatttccaag gcctccaatt 2760 tcgagcgttt cgtcttcgac ctagtgggcc gtgacgccgc caaggtgcgc gagctatggg 2820 gcaaggtgga tgcgggcggc agtttcgacc ttaacgacgg tggctggttt gccaaggtag 2880 cggattacgg cttcgtttcc ggcagcagca accatgccaa ccgcatgcag accatcaagg 2940 cgacgcatga gcgctacggt gtcaccattg atacccacac cgcggacggt ctcaaggtgg 3000 cgctggaaca ccgcgaggca ggtaccccga tgctggtgct ggaaaccgca ttgccggcca 3060 aattcgagga cgcgattgtc gaggcgttag ggcataaacc cgagcgtccg cacagcctgg 3120 aaggcctgga atccctgcct cagcgctttg aggtgatgga ggccgatgca gccgtcatca 3180 aacagttcat tgttgagcat atttgatcgg gagatgtcat gaaggtctat cacgacttga 3240 tgcgccacgt actggagcac ggccacaaga aggaagaccg taccggtacc ggcaccttat 3300 cagtgtttgg ctaccagatg cgttttgact tggccgaggg cttcccgcta cttaccacca 3360 agaaggtgca cctgaaatcc atcatccatg agttgctttg gttcctgcag 3410 // |
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