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EBI DbfetchID D13255; SV 1; linear; genomic DNA; STD; PRO; 2065 BP. XX AC D13255; D01187; M74130; S39465; XX DT 25-SEP-1992 (Rel. 33, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 11) XX DE Bacillus sp. KSM-1876 gene for neopullulanase, complete cds. XX KW neopullulanase. XX OS Bacillus sp. KSM-1876 OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2065 RA Igarashi K.; RT ; RL Submitted (27-SEP-1991) to the EMBL/GenBank/DDBJ databases. RL Kazuaki Igarashi, Kao Corporation, Tochigi Research Laboratories; 2606 RL Akabane, Ichikai, Haga-gun, Tochigi 321-3497, Japan (Tel:0285-83-7400, RL Fax:0285-83-7403) XX RN [2] RP 1-2065 RX PUBMED; 1368334. RA Igarashi K., Ara K., Saeki K., Ozaki K., Kawai S., Ito S.; RT "Nucleotide sequence of the gene that encodes a neopullulanase from an RT alkalophilic Bacillus"; RL Biosci. Biotechnol. Biochem. 56(3):514-516(1992). XX FH Key Location/Qualifiers FH FT source 1..2065 FT /organism="Bacillus sp. KSM-1876" FT /strain="KSM-1876" FT /mol_type="genomic DNA" FT /clone="pBH216" FT /db_xref="taxon:72413" FT -35_signal 96..101 FT -10_signal 119..124 FT repeat_region 142..155 FT /rpt_type=INVERTED FT /rpt_unit_range=149..155 FT /rpt_unit_range=142..148 FT misc_feature 162..176 FT /note="possible catabolite-repression operator" FT RBS 219..224 FT CDS 230..1981 FT /codon_start=1 FT /transl_table=11 FT /product="neopullulanase" FT /db_xref="GOA:Q57482" FT /db_xref="HSSP:1J0H" FT /db_xref="InterPro:IPR004185" FT /db_xref="InterPro:IPR006047" FT /db_xref="InterPro:IPR006589" FT /db_xref="InterPro:IPR013780" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR013783" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/TrEMBL:Q57482" FT /protein_id="BAA02521.1" FT /translation="MLREAIYHRPKNNFAYAYDKETIHIRLRAKKGDLTKAYLIFGDPY FT QWTDEGWKNDTKPMVFEGSDELFEYWFVEVKPEFRRLRYGFELHSDDQCLVYGEDGFKE FT KDPKNAHFYFVFLFLNGNDVFTAPEWVKDTIWYQIFPERFPNGNPSLNPEGTLPWGSVE FT PKWDTFFGGDLEGIINNIEYLKELGINGIYLTPIFKARTNHKYDTVDYMEIDPQFGDKQ FT TFKKLIEVCHDNGIRVMLDAVFNHSGFYFEQFQDVLEHQEKSKFRDWFHLREFPVIPLP FT EPNYDTFAFTHQMPKLNTANPEVKEYLLNVGRYWVEEFDIDGWRLDVANEVDHQFWREF FT RNTIKSIKPDVYILGEVWHDSMPWLQGDQFDAVMNYPFTQAVVEFIPKNEIDSKQFEQQ FT LTKLLTSYPQNVNEVAFNLLGSHDTPRILTICEGNKDKLKLLLLLQFSFIGTPCIYYGD FT EIGLTGGNDPGCRKCMVWDESDQDSELLNFFRSLIALRRNYPAFGNKGLVEVMETNRET FT NHIIYAKKSSQETIVIAINNSSEKIKVDLPHEFNDRKSIDIWNNEPFPKMSEVELNPWG FT FAIIKLT" FT repeat_region 2002..2015 FT /rpt_type=INVERTED FT /rpt_unit_range=2002..2008 FT /rpt_unit_range=2009..2015 FT /note="The sequence here and at 142..155 are the same FT palindrome." XX SQ Sequence 2065 BP; 691 A; 285 C; 430 G; 659 T; 0 other; aggccttcca tttgttcact gaacaaggaa aagcctcttt tgtttatgaa taaaaatgaa 60 cctctacccc aattataaca atttacagaa aattgttgaa agtcgcctat caataaatta 120 taatactctt aaccgacaat agttaaccgg ttaacctttt atgtaagcgt tttaatagtg 180 aatcatatgg ggtgtgaaaa gttagataat ggatttatag gggggcttta tgttaagaga 240 agcgatttat cacagaccaa agaataattt tgcctatgcg tatgataaag agacaatcca 300 tattcgtctt agagccaaga aaggtgattt gactaaagcg tacttaatat ttggtgatcc 360 ttatcagtgg acggacgaag ggtggaaaaa cgatacaaaa ccgatggtgt ttgaaggaag 420 cgatgagtta tttgagtatt ggtttgtaga ggttaaacct gagtttagac ggttacgtta 480 tggttttgaa cttcattctg acgatcaatg tcttgtttat ggagaagatg gtttcaaaga 540 aaaagatcct aagaatgcac atttttattt tgttttcctt tttttgaatg gaaatgatgt 600 atttactgca ccggagtggg taaaagatac aatttggtat caaatctttc ctgagagatt 660 tccaaatgga aacccatctt taaatcctga aggtactttg ccatggggaa gcgtggagcc 720 aaagtgggat acttttttcg gtggtgattt ggaagggatt attaataata ttgagtattt 780 gaaggaatta ggtattaatg gaatctattt aacgccgatt ttcaaagcta gaacgaatca 840 taagtatgac acggttgatt atatggagat tgacccgcaa tttggggaca aacaaacttt 900 taagaagttg attgaagttt gtcatgataa tgggatacgg gtaatgcttg acgctgtatt 960 caaccatagt ggtttctatt ttgaacagtt tcaagatgtt ttggaacacc aagaaaaatc 1020 taaatttaga gattggtttc atttgaggga atttcctgtg attcctttac ctgaaccaaa 1080 ttatgataca tttgctttca cacatcagat gcctaagtta aatactgcaa accctgaagt 1140 aaaagaatat ttacttaatg ttggacggta ctgggtagag gaatttgata tagatggatg 1200 gcgtttagat gttgcgaatg aagttgatca tcaattctgg agagagttca ggaatacaat 1260 taaatccatt aaacctgatg tgtatatact tggtgaagtt tggcatgact ccatgccctg 1320 gttacaagga gaccaattcg atgctgttat gaattatcct tttactcaag ctgtagtgga 1380 attcattcca aaaaatgaaa tagattcaaa acaatttgaa cagcagttaa caaaacttct 1440 tacttcatat ccccaaaatg tgaatgaagt tgccttcaac ttattgggga gtcatgatac 1500 tccaaggatc ttgaccattt gtgagggaaa taaagataaa ttgaaactac tcttgctgtt 1560 acaattttct tttataggta caccatgtat atattatggg gatgaaatag gtttaacagg 1620 tggtaacgat ccaggttgtc gaaaatgtat ggtgtgggat gaaagtgatc aggattctga 1680 actgttaaat ttttttagaa gtttaatagc tttacgaaga aactatcctg cttttggtaa 1740 taaagggctg gtagaagtga tggaaacaaa tagagaaaca aatcatatta tttatgcgaa 1800 aaaatcatcc caagaaacta ttgtaatagc cattaataat tcaagtgaaa aaattaaagt 1860 ggacttgcca catgagttta acgatagaaa atcaatagat atttggaaca atgaaccttt 1920 cccaaaaatg tctgaggtgg aattaaaccc atggggattt gctattatta aacttactta 1980 atgttaatcc ttgattggca agttaaccgg ttaaccacta cgtgaaggag gtgataatga 2040 attaattcgc agtgtatatg ttaac 2065 // |
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