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EBI DbfetchID D13229; SV 1; linear; genomic DNA; STD; PRO; 6947 BP. XX AC D13229; XX DT 17-FEB-1993 (Rel. 35, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Pseudomonas putida plasmid NMH102-2 PHT1-5 genes, complete cds. XX KW PHT plasmid; phthalate degradation. XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OG Plasmid NMH102-2 XX RN [1] RP 1-6947 RA Oshima Y.; RT ; RL Submitted (17-SEP-1992) to the EMBL/GenBank/DDBJ databases. RL Yasuji Oshima, Osaka University, Department of Biotechnology; 2-1 RL Yamadaoka, Suita, Osaka 565, Japan (Tel:06-877-5111, Fax:06-876-2250) XX RN [2] RP 1-6947 RA Nomura Y., Nakagawa M., Ogawa N., Harashima S., Oshima Y.; RT "Genes in PHT plasmid encoding the initial degradation pathway of phthalate RT in Pseudomonas putida"; RL J. Ferment. Bioeng. 74:333-344(1992). XX FH Key Location/Qualifiers FH FT source 1..6947 FT /organism="Pseudomonas putida" FT /plasmid="NMH102-2" FT /mol_type="genomic DNA" FT /db_xref="taxon:303" FT CDS 715..2070 FT /codon_start=1 FT /transl_table=11 FT /gene="PHT1" FT /db_xref="GOA:Q05181" FT /db_xref="InterPro:IPR011701" FT /db_xref="UniProtKB/Swiss-Prot:Q05181" FT /protein_id="BAA02509.1" FT /translation="MTTTAATDAVPHLLQRSHERIEKVYRKVTLRLMTFIFVAWVLNYL FT DRVNISFAQVYLKHDLGMSDADLRTRRKLVFHRLHRIGNTQYAYLQKIGARLTITRIMV FT LWGLISASMAFMTTPTEFYIARALLGAAEAGFWPGIILYLTYWYPGARRARITSRFLLA FT IAAAGIIGGPLSGWILTHFVDVMGMKNWQWMFILEGLPAAVMGVMAYFYLVDKPEQAKW FT LDDEEKSIILDALAADRAGKKPVTDKRHAVLAALKDPRVYVLAAGWATVPLCGTILNYW FT TPTIIRNTGIQDVLHVGLLSTVPYIVGAIAMILIARSSDIRLERRKHFFFSIAFGALGA FT CLLPHVVDSAIISITCLAMIAVSYFGAAAIIWSIPPAYLNDESAAGGISAISSLGQIGA FT FCAPIGLGWINTVTGSLAIGLTIIGALVLAGGMAVLIAVPANALSEKPLTDE" FT CDS 2183..3157 FT /codon_start=1 FT /transl_table=11 FT /gene="PHT2" FT /db_xref="GOA:Q05182" FT /db_xref="HSSP:2PIA" FT /db_xref="InterPro:IPR000951" FT /db_xref="UniProtKB/Swiss-Prot:Q05182" FT /protein_id="BAA02510.1" FT /translation="MSSSEQLDDGFTGLKVIAKTEIAQGIFRFELAHPQGMLLPAFTAG FT AHLRVRVPNGSIRNYSLSNDPQERERYVIAVKRDANGRGGSVSMADDIEAGDLLPVATP FT QNEFELIENARQFIFVAGGIGITPILSMMRHLKASTDLPFKLYYCTRNPELTAFRDELL FT GAEFANTVVIHHDFGNRADAYDFWPVFDKPSSGTHVYCCGPRPLMDSVLDMTGHWPPGS FT IHFESFGVDQSRFAENRPFSVTLGRSGIDLEIPVDRSILEVLRDNGIRAPSSCESGTCG FT SCRTRLIEGDVEHRDMVLREDEQHDQIMICVSRARNDVLVLDL" FT CDS 3204..4523 FT /codon_start=1 FT /transl_table=11 FT /gene="PHT3" FT /db_xref="GOA:Q05183" FT /db_xref="InterPro:IPR015881" FT /db_xref="UniProtKB/Swiss-Prot:Q05183" FT /protein_id="BAA02511.1" FT /translation="MLTPEENLLLCRVEGDAPMGQMMRRHWTPVCLLEEVSEPDGTPVR FT ARLFGEDLVVFRDTDGRVGVMDEYCPHRRVSLIYGRNENSGLRCLYHGWKMDVDGNVVE FT MVSEPAASNMCQKVKHTAYKTREWGGFVWAYMGPQDAIPEFVPPAWAPHEHVRVSIAKA FT IIPCNWAQILEGAIDSAHSSSLHSSDFVPARVGGAEATSKNWLRPSTDKAPRMQVERTS FT YGFRYAALRRPIQNAATSEYVRSTVFVAPATALIPPNNLYNVANINVPIDDTHTAFYFM FT AWGNPDNTPETETWRKFLGQQVGIDLDDSYRPLRNDGNRFFQDREAMKNGNFTGIKGFP FT NQDIAMWVTMGPIADRSDERLGASDLAVVEFRRVMLDALAAFQAGESAIGTGEKAIPSR FT ICSFQAIVSKDIDWRDYQARYVWALDDANIVAEPDYEVHT" FT CDS 4520..5752 FT /codon_start=1 FT /transl_table=11 FT /gene="PHT4" FT /db_xref="GOA:Q05184" FT /db_xref="InterPro:IPR004104" FT /db_xref="UniProtKB/Swiss-Prot:Q05184" FT /protein_id="BAA02512.1" FT /translation="MMASHAESTARLRLGVVGLGRAFTLMLPTFLADRRVQLVGACDPR FT EQARRQFERDFDAPAYETIEDLAADSNVDALYIASPHQFHAEHTRIAAANRKHVLVEKP FT MALSLDECDRMIADCAEAGVKLIVGHCHSFDTPYLRTRELIGSGEFGAVKMIQALNYTD FT YLQRPRRPEELSTAEGGGAVFSQAAHQVDVVRLLAGSRATRVRAAVGNWDPARPTEGAY FT TATLWFENGAFASITYNGYGHFDSDEWMDWVGEMGKPKNPEAYGGARRLLQQVQTADEE FT ARLKAEGTYGGTRYVSPSPTDDATAFQHFGPIIVSCEGGDLRPMADAVMIYRPHSRDRM FT MLERPTVPRSEVIDELYLAQFYGVTPLHDGEWARDTLEICLAMLRSSEEQRDITLGVNV FT EDTQWRENPSS" FT CDS 5728..6504 FT /codon_start=1 FT /transl_table=11 FT /gene="PHT5" FT /db_xref="GOA:Q05185" FT /db_xref="UniProtKB/Swiss-Prot:Q05185" FT /protein_id="BAA02513.1" FT /translation="MAREPIIMNKLNLSIAVGNYVRIRPLVDGEVQIDGVDPIFMLQDP FT EEIFFRAFRHADYDICELSLSSYSVKTAAGTSPYIAVPVFPSRAFRHTSIYIRNDRGIE FT SAADLKGKRIGVPEYQLTANVWVRLFLEEDHGLKASDVTWVRGGYEETGRLEKIVLKLP FT ADVIVENAPETETLSGMLASGELDAVIGPRAPSCFTQGHPKVSYLYRDPQGAASDWYRA FT LSYSRSCTCWGSGARWPSSTLGYPGPLPKHSRSPSP" XX SQ Sequence 6947 BP; 1472 A; 1981 C; 1941 G; 1553 T; 0 other; gaattcgctt ctgcgtttgc gagattctct atcaccgtca atagaacgtg tcgtgctgtt 60 tcgacgtctt gctcctcgag ccccggccac actcacctcg ttgacttcca gcgccagagg 120 aatcaacaca tccttcagtc gccgtccttc atcggtgagg aagacgtgaa catttttctt 180 gttatccggc atctgctgcc ggacgacgta gcccagtttt ttccatcgcc ttcaccgcag 240 cgaaagtcgt aggttccatc agtccagcct gagtgctgag ttgtttttgg gctaatccgt 300 ctgtctccca gaggatccgc aagacgccca atgaccaaag gatacgtcgt gttcggtcaa 360 ccgcatctgc agtgctctga ccagcgcgcg agttgcatcc ttgaccaggt gggccagacg 420 gtcatcaggt acaacatcgt gccaatgcct gagtatttcc tgggtttcag gttgtttgtg 480 gcctaaaggc tttcgtgcag tcatatcaag gtccgatctc atcacctgcg taagcgatac 540 taagcataac gcgttgcctc aacacatgcg ctagatcaaa aaatacttag agatcgttct 600 ttttttgatt gcgagagaag aaaattcgcc atattctctt agagacctaa taaaaaagca 660 caccaagccc ccccatggct ttggtcgtgc tacacaaagt ccgaggcgtg cacaatgaca 720 acaacagcag ctacagatgc cgtgccccat cttctccagc gcagccatga gcgcatcgag 780 aaggtctata gaaaagtcac actacgtttg atgaccttca tcttcgtggc ctgggttctc 840 aattatctgg atcgcgtgaa catcagcttc gcgcaggttt acctgaagca cgatttaggc 900 atgagcgatg ctgacctacg gactcggcgt aagcttgttt ttcatcggct acatcgcatt 960 ggaaataccc agtacgctta cctgcaaaag attggtgccc ggttgacgat cacccggatc 1020 atggtgctgt ggggcttgat ctcagcgtcc atggcgttca tgacaacgcc cactgagttc 1080 tacattgccc gcgccctgtt gggcgctgcc gaagctggct tctggcctgg aatcattctt 1140 tatctgacct attggtatcc aggtgccagg cgtgctcgca tcacctcgcg cttcttgttg 1200 gccatcgccg cggcgggaat catcggcggt cccctgtccg gttggatact cacgcatttc 1260 gtcgacgtca tgggaatgaa aaactggcag tggatgttca tcctggaagg tcttcccgct 1320 gccgtaatgg gtgtgatggc ctatttctat ctggtggaca aacccgagca ggccaagtgg 1380 ctcgacgacg aggaaaaatc cattattctc gacgcgctgg ccgctgaccg cgcaggcaag 1440 aagcccgtta ccgataagcg tcatgctgtg ctggctgcgc tcaaggatcc gcgcgtatac 1500 gtccttgctg ctggttgggc caccgtgccg ctctgtggca cgatcctcaa ttactggacg 1560 cctaccatca tccgcaacac cggcatccag gatgtattgc atgtcggatt gctttccacc 1620 gtgccataca tcgtgggggc gatcgccatg attctcattg cgcgcagctc cgacattcgt 1680 ctggagcgtc gcaagcactt ctttttcagt atcgccttcg gtgccttggg cgcctgcctg 1740 ctgcctcacg ttgtggactc ggccattatt tcaattactt gcctggcaat gattgcagtg 1800 tcgtacttcg gtgccgcagc gatcatctgg tcgattcctc ctgcttatct taatgatgag 1860 tccgccgcag gggggatttc ggcgatcagt tctcttggtc agatcggagc gttctgtgcg 1920 cccatcggac tgggctggat caacacagtc accggcagcc tcgcaatcgg actgacgata 1980 atcggggccc tggtcctggc gggtggtatg gcggttctga ttgcggtgcc agctaacgcc 2040 ttgagcgaaa agccacttac cgacgaatag gatcgagcag tctgattggc cgaatcacgc 2100 gcccaaaaaa tgttagatat ctatgcaact ctcggtgcgc gggcgtgttc agcccgcgac 2160 cggatgtaat gaggaaacag cgatgagtag ttccgaacag ctcgatgatg ggttcactgg 2220 cctcaaggtg atcgccaaaa ccgaaattgc ccaaggcatt ttccgcttcg aattggccca 2280 cccgcagggg atgcttctgc ccgcctttac ggccggagcg cacctcaggg taagggtgcc 2340 caacggaagt atccgcaatt actcattgag caacgacccg caagagcgtg agcgttatgt 2400 cattgctgtc aagcgtgacg ccaacggacg aggcggttcg gtgagcatgg ccgatgatat 2460 cgaggcaggc gacttgctac cggtggcaac tccgcaaaat gagttcgaac tgatcgagaa 2520 tgcccgacag ttcatcttcg tcgccggagg catcggtatt accccgatcc tgtcgatgat 2580 gcgtcacctg aaggccagca ccgacctccc cttcaaactt tactactgca cccgtaatcc 2640 ggaactcacc gccttcaggg atgagctgct gggggccgaa tttgccaaca cggtagtcat 2700 tcaccacgac ttcggcaatc gggcagacgc ttacgatttc tggcctgttt tcgacaaacc 2760 gagcagcggt acacatgtgt actgctgtgg cccccggcca ttgatggaca gcgtgctgga 2820 catgacgggg cactggccac ccggctccat acattttgaa agcttcggcg tcgaccagag 2880 tcgcttcgcc gaaaaccggc ctttttcggt gacccttggc cggagcggaa tcgacctcga 2940 aattcccgtc gaccgctcga ttctggaggt tctgcgtgac aacggtatcc gggcgccaag 3000 ttcttgtgag agcggcacct gcggctcttg ccgtacccgt ctgatcgaag gtgacgtcga 3060 gcatcgcgac atggtgctgc gtgaggacga acagcacgat cagatcatga tttgcgtttc 3120 ccgagccaga aatgacgtgc tggtactcga cctctaggcg agaccttttt tacaaacgat 3180 tagttgaacg ctggagatat tacatgttga ccccagaaga gaacctgttg ctctgccgcg 3240 tcgaaggcga cgcaccgatg ggccagatga tgcgccgtca ctggacgcca gtgtgtttgc 3300 tcgaagaagt cagcgagccc gacggcacgc ctgtcagggc ccgtctgttc ggcgaggacc 3360 tggtcgtgtt tcgcgacacc gatggtcgcg tgggagttat ggacgagtat tgcccgcatc 3420 gccgcgtgtc actgatctac ggccgcaatg aaaactcggg attacgctgc ctgtatcacg 3480 gctggaagat ggacgtggac ggtaatgtgg tggaaatggt ttccgagcct gccgccagca 3540 acatgtgtca gaaggtcaag cacacggcct acaaaacccg cgagtggggc ggtttcgtct 3600 gggcctacat ggggccacag gacgcaattc ccgaatttgt gccgccggcc tgggcacccc 3660 atgagcatgt tcgagtgagc atcgccaagg caatcattcc ctgcaactgg gcgcagatcc 3720 tggaaggcgc catcgactcg gcacacagct caagcctgca ctcatccgac ttcgttccag 3780 cccgggtcgg gggggcagaa gcaacctcga aaaactggct aagaccgtcc acggacaaag 3840 cgccgcgcat gcaagtcgag cgtaccagct atggcttccg ctatgccgct ctgcgccgtc 3900 ctatccagaa tgccgccacc agcgagtatg tacgctctac ggtgttcgta gccccggcca 3960 ccgcactgat cccaccgaat aatttgtaca acgtcgcgaa tatcaacgta cccatcgacg 4020 acacccacac tgccttctat ttcatggcct ggggaaatcc cgataacact ccggaaacag 4080 agacctggcg aaagtttctc ggccagcaag tcggcatcga tctggacgac agttaccgac 4140 cgctacgcaa cgacgggaac cgttttttcc aggaccgtga agccatgaaa aacggcaact 4200 ttacgggcat caagggtttc cccaatcagg atatcgccat gtgggtgacc atggggccca 4260 tcgccgatcg ctccgatgag cgcttgggcg ccagtgactt ggcggtggtg gagttccgcc 4320 gggtcatgct ggatgccttg gcagcctttc aggcgggtga atccgctatt ggcacaggtg 4380 agaaagcgat cccttccaga atctgctcgt tccaggcgat cgtgtccaag gatatcgact 4440 ggcgtgatta tcaagctcgt tacgtatggg ccctggatga tgccaacata gtcgcagagc 4500 ctgattacga ggttcatacg tgatggccag ccatgctgaa tccacggctc ggctacgtct 4560 gggggtggtc ggtctgggcc gggcattcac cctcatgctg cctaccttcc tggccgatcg 4620 ccgcgtccag ttggtgggcg cctgtgaccc tcgtgaacaa gctcgcaggc aattcgaacg 4680 ggatttcgat gcgcctgcat acgagaccat cgaagacctg gcagccgaca gcaatgtcga 4740 cgccttatac atcgccagcc cacatcagtt tcacgccgaa cacacccgta tagctgcggc 4800 taaccgcaag cacgtgctgg tcgaaaaacc catggcgttg tcgttggacg agtgcgaccg 4860 gatgatcgcc gattgcgccg aggcgggtgt gaagttgatc gttgggcatt gccacagctt 4920 cgataccccg tacctgcgta cccgggagct gattggtagt ggcgagttcg gcgcggtcaa 4980 gatgattcaa gcgttgaact acaccgacta cctgcagcgt cctcgccgcc cggaagaact 5040 ctcgaccgct gaaggcggcg gagcagtatt cagccaggcc gcccaccaag tagacgtggt 5100 ccgtttgctg gctggcagtc gcgcgacccg tgtccgcgcg gcagtgggta actgggaccc 5160 cgcccggcct actgaaggcg cctacacagc cactttatgg ttcgagaatg gggcattcgc 5220 ctccattacc tacaacggct acggacactt cgactccgac gagtggatgg actgggttgg 5280 agaaatgggc aagcccaaaa accccgaagc ttacggcggc gcacgtcggt tattgcagca 5340 ggtgcaaacg gcggatgagg aggcgcggct caaggctgag ggcacttatg gcggcacacg 5400 ctacgtcagc ccctccccta ccgacgacgc gacggccttc cagcatttcg gcccgattat 5460 tgtgtcgtgc gaaggtggcg accttaggcc aatggcggat gccgtcatga tttaccggcc 5520 gcatagtcgt gatcgaatga tgcttgagcg gccaacggtg ccgcgttccg aagtcataga 5580 cgaactgtat ctagcgcagt tctacggggt cactccgttg catgacggcg agtgggctcg 5640 tgacacgttg gagatctgtc tcgccatgtt gcgatccagc gaagaacaac gcgacatcac 5700 actgggtgtg aatgttgaag atactcaatg gcgcgagaac ccatcatcat gaacaagttg 5760 aacttatcta ttgctgtggg caactacgta cgtatccgac cattggtgga cggcgaagta 5820 cagatcgatg gggtcgaccc gatctttatg ctgcaagatc ccgaagagat cttctttcgc 5880 gcatttcgcc atgctgacta cgacatctgc gagttatcgt tgagcagcta cagcgttaaa 5940 accgcggctg gcaccagtcc ctatattgct gtccccgtct tcccgtcccg cgcgtttcga 6000 cacacctcga tctatatccg caatgatcgc ggaatcgagt ccgcggcaga cctcaagggc 6060 aaacgtatcg gtgtgccgga gtaccagttg acagcgaacg tatgggttcg actgttcctg 6120 gaagaagacc atggtttgaa agcgtctgac gtcacctggg tccgtggcgg gtatgaagag 6180 accgggcgtc tggagaaaat tgttctgaag ctgccggcgg atgtaatcgt cgagaacgca 6240 ccggagactg aaacgctttc tggcatgctc gcctctggtg agctggatgc cgtgatcggt 6300 ccacgtgcac cttcctgttt cacccagggc catcctaagg tcagctatct ctatcgtgat 6360 cctcagggag cggcctcgga ctggtatcgc gcactcagct attcccgatc atgcacgtgc 6420 tggggatccg gcgcacgttg gccgagcagc acccttggtt acccggggcc gttgccaaag 6480 cattcgagaa gtccaagtcc gtagcgctgc acaaattgag cgacacctcc gccaccaagg 6540 taacgttacc gtttatcgag gatcagttaa gagcggcgcg ccagttgctc ggtgaagact 6600 tctggtccta tggtttcgaa gctaaccgcc acgtactcag tcgcttcctg gaacgccacc 6660 acgccgaagg cctgtcgtca cgcttgcttc agcctgaaga gctgttccat ccagcttcgt 6720 tggagagttt caagatttaa cgaaagtcct ggcgaaggtt tacccctcga acaaacgtta 6780 tagggcattt cgtgtactta gcatggtaat aaattttgtg gctgctccta tcgtgcggtt 6840 agatggcggc cacaactttt ttgcacgctg cgtatgttgg cgatcgccat gctcgaaaac 6900 gcacgtatat ataaggccag gtaaaaacat acgtgcatgc ggaattc 6947 // |
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