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EBI DbfetchID D12706; SV 1; linear; genomic DNA; STD; PRO; 2416 BP. XX AC D12706; XX DT 14-APR-1993 (Rel. 35, Created) DT 26-FEB-2008 (Rel. 94, Last updated, Version 6) XX DE Pseudomonas aeruginosa genes for negative and positive regulators of pyocin DE genes, complete cds. XX KW . XX OS Pseudomonas aeruginosa OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 1-2416 RA Shinomiya T.; RT ; RL Submitted (24-JUL-1992) to the EMBL/GenBank/DDBJ databases. RL Contact:Tomoyuki Shinomiya Mitsubishi Kasei Institute of Life Sciences, RL Molecular Biology; 11, Minamiooya, Machida, Tokyo 194, Japan XX RN [2] RP 1-2416 RX PUBMED; 8444788. RA Matsui H., Sano Y., Ishihara H., Shinomiya T.; RT "Regulation of pyocin genes in Pseudomonas aeruginosa by positive (prtN) RT and negative (prtR) regulatory genes"; RL J. Bacteriol. 175(5):1257-1263(1993). XX FH Key Location/Qualifiers FH FT source 1..2416 FT /organism="Pseudomonas aeruginosa" FT /strain="PAO" FT /isolate="PAO1" FT /mol_type="genomic DNA" FT /db_xref="taxon:287" FT CDS 53..823 FT /codon_start=1 FT /transl_table=11 FT /gene="prtR" FT /product="negative regulator of pyocin genes" FT /function="a plausible DNA binding protein with FT helix-turn-herix motif" FT /db_xref="GOA:Q06553" FT /db_xref="HSSP:3CRO" FT /db_xref="InterPro:IPR019759" FT /db_xref="UniProtKB/Swiss-Prot:Q06553" FT /protein_id="BAA02198.1" FT /translation="MDKSTQIPPDSFAARLKQAMAMRNLKQETLAEAAGVSQNTIHKLT FT SGKAQSTRKLIEIAAALGVSPVWLQTGEGAPAARSAVSVADGSPLVLEPLHPWDSDTPL FT DEDEVELPLYKEVEMSAGAGRTAVREIEGRKLRFSYATLRASGVDPSAAICAQLTGNSM FT EPLIMDGSTIGVDTATTHITDGEIYALEHDGMLRVKFVYRLPGGGIRLRSFNREEYPDE FT EYSPEDMRSRQISMIGWVFWWSTVRHRRGPSLVR" FT repeat_region 846..859 FT /rpt_type=DIRECT FT repeat_region 871..884 FT /rpt_type=DIRECT FT RBS 910..915 FT CDS 923..1237 FT /codon_start=1 FT /transl_table=11 FT /gene="prtN" FT /product="positive regulator of pyocin genes" FT /db_xref="GOA:Q06552" FT /db_xref="UniProtKB/Swiss-Prot:Q06552" FT /protein_id="BAA02199.1" FT /translation="MQPSIAPSTPIPRQETVELVYRIFGDVLVPLEQVRERWFRNLNKE FT NFSKALACGRIALPVTTLDDSHKAMQFVALDHLAAYVDQRANQAGSARRAQPGADSIAS FT " FT stem_loop 1499..1536 FT CDS complement(1556..>2416) FT /codon_start=1 FT /transl_table=11 FT /gene="trpE" FT /product="anthranilate synthetase" FT /note="3' harf of trpE" FT /db_xref="GOA:P20580" FT /db_xref="HSSP:1K0G" FT /db_xref="InterPro:IPR015890" FT /db_xref="UniProtKB/Swiss-Prot:P20580" FT /protein_id="BAA02200.1" FT /translation="LEAAQGREPAFRASFTREDYENAVGRIKDYILAGDCMQVVPSQRM FT SIEFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVEDGLVTVRPIAGTR FT PRGINEEADLALEQDLLSDAKEIAEHLMLIDLGRNDVGRVSDIGAVKVTEKMVIERYSN FT VMHIVSNVTGQLREGLSAMDALRAILPAGTLSGAPKIRAMEIIDELEPVKRGVYGGAVG FT YLAWNGNMDTAIAIRTAVIKNGELHVQAGGGIVADSVPALEWEETINKRRAMFRAVALA FT EQSVE" XX SQ Sequence 2416 BP; 453 A; 817 C; 726 G; 420 T; 0 other; gtcaacacct aggctcttta cagaaaatcc atcggtctgt agattgccga gcatggacaa 60 gagcacccag atcccgcccg acagcttcgc cgctcgcctc aagcaggcca tggcgatgcg 120 caacctgaag caggaaaccc tcgccgaagc ggcaggggtt tcgcagaaca ccattcacaa 180 gctgacctcg ggcaaggccc agagcacccg caagctgatc gagatcgcgg cggccctggg 240 cgtctcgccg gtctggctgc agaccggcga aggcgctcca gccgcgcgca gtgccgtgtc 300 cgtggccgat ggcagcccat tggtgctgga accgctgcat ccgtgggaca gcgacacacc 360 gctggacgaa gacgaagtgg aactgccgct gtacaaggaa gtggagatgt ccgccggcgc 420 cggacgcact gcggtgcgcg agatagaggg gcgcaagctg cgtttttcct acgccacgct 480 gcgtgcctcg ggcgtcgatc cgtcggcggc gatctgcgcc caactcaccg gcaacagcat 540 ggaaccgctg atcatggatg gctccaccat cggcgtggac accgccacca cccatatcac 600 cgatggcgag atctacgccc tcgaacatga cggcatgctg cgggtgaagt tcgtctatcg 660 cctgcccggc ggcggcattc gcctgcgcag cttcaaccgc gaggaatatc cggacgagga 720 gtactcgccg gaggacatgc gcagccgcca gatcagcatg atcggttggg tcttctggtg 780 gtccaccgta cgccaccggc gcggcccgtc cctggtgcgg tgagcgcctg gcgctcgccc 840 gctctacaac ttcagctatt gaacaagaaa acaacctgag ctatatttcc ctcaacgacg 900 tgtagccgca ggagggaata ccatgcagcc atccatcgcc cctagcactc cgattccacg 960 ccaggaaacc gtggaattgg tctaccgcat cttcggcgac gtcctggtgc cgctggagca 1020 ggttcgcgaa cgctggtttc gcaacctgaa caaggaaaac ttcagcaagg ccctggcatg 1080 cgggcggatc gccctgccgg tgacaaccct cgacgatagt cacaaggcca tgcagttcgt 1140 ggccctcgat cacctcgccg cctacgtcga ccagcgcgcg aaccaagccg gaagcgcccg 1200 tcgagcacag ccgggcgctg acagcatcgc atcctgagcg gcccgccgtt gccagcatcg 1260 tagggagcga caccatgctc cgcctggcgg atatcgcgtg gtgatgtccc ccctacgaaa 1320 gcgccttctg cgtgggcaag taatgccgaa ggcgtcatcc gccaaccgga catatcgatc 1380 acttcacctt gaggcgggtt cggcgtcctg ctccctggcg gatatcgcgc cgcgatattc 1440 gccctacgaa agcggtgccg cgtagggcga agccaatgcg tgggtccacg ccgggcacaa 1500 aaaaggcgga tgacgcaaac gccatccgcc ttcttttctt ccgcctcgcg ccgtcttatt 1560 cgacgctctg ctcggccagc gccacggcgc ggaacatcgc ccggcgcttg ttgatggttt 1620 cttcccactc cagcgcgggc accgagtcgg caacgatacc gccgccggcc tgcacgtgga 1680 gttcaccgtt cttgatcacc gcggtgcgga tggcaatggc ggtgtccatg ttgccgttcc 1740 atgccaggta gccgaccgcg ccgccgtaga ctccacgctt gaccggctcc agctcgtcga 1800 tgatctccat ggcgcggatc ttcggcgcgc cggatagagt gcccgccggc agaatcgccc 1860 gcagcgcgtc catcgcgctg agcccctcgc gcaattgccc ggtgacgttg gacacgatgt 1920 gcatgacgtt ggagtaacgt tcgatcacca ttttttcggt gaccttcacc gcgccgatat 1980 cggacacccg ccccacgtcg ttgcgcccca ggtcgatcag catcaggtgc tcggcgatct 2040 ccttggcgtc cgacagcaga tcctgctcca gtgccaggtc ggcctcttcg ttgatcccgc 2100 gcggacgggt accggcgatc gggcgcaccg tcaccaggcc atcctcgacc cgtaccagca 2160 cctccggcga gctgcccacg acatggaagt cgccgaagtt gaagaagtac atgtagggcg 2220 tcggattgaa acagcgcagc gcgcggtaca ggtcgatggg cgccgccttg aattcgatgg 2280 acatgcgctg cgacggcacc acctgcatgc agtcgccggc caggatgtag tccttgatcc 2340 ttcctaccgc gttttcatag tcctcgcggg tgaagctggc acgaaacgcc ggctcacgac 2400 cctgggccgc ctcgag 2416 // |
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